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MOLECULAR EVALUATION OF BULL SEMEN IN RELATION TO CERTAIN FERTILITY MARKERS
(Assam Agricultural University, Khanapara, Guwahati, 2016-07) BORGOHAIN, INDRANI; DUTTA, D. J.
The present study was conducted for molecular evaluation of bull semen and to study its relationship with different semen characteristics. Fresh semen samples were collected from six breeding bulls. A total of six ejaculates were collected from each of six healthy breeding Jersey bulls at 4 days interval. Immediately after collection, the samples were subjected to physio-morphological and biomolecular evaluation. Percentage of Hypo-osmotic Swelling Test (HOST) positive spermatozoa and acrosome-intact sperms increased with an increase in initial sperm motility. Biochemical evaluation indicated that seminal plasma protein, sperm protein and sperm cholesterol concentration increased with higher motility. The protein profiling revealed the highest band intensity of a 25 kDa protein in both seminal plasma and sperm membrane of all the bulls. Whereas, proteins of 104-92 kDa molecular mass were absent in the bulls with initial sperm motility below 80 per cent in both seminal plasma and sperm membrane. Expression of Chaperonin Containing T-complex protein 1, subunit 8 (CCT8) gene was found to be negatively correlated with sperm motility, whereas the expression of Adenylate Kinase 1 (AK1) gene did not show any significant relationship with sperm motility.