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Author: BANIA, BIRAJ KUMAR × End date: 2017 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    TECHNOLOGY VALIDATION OF SEMEN PRESERVATION, SYNCHRONIZATION OF OESTRUS AND ARTIFICIAL INSEMINATION IN PIG
    (Assam Agricultural University, Khanapara,Guwahati, 2017-07) BANIA, BIRAJ KUMAR; Das, K.K.
    A total of 36 ejaculates from six boars (three Hampshire and three Rani) aged one and half to two years and maintained at National Research Centre on Pig, ICAR, Rani, Guwahati, collected twice in a week by gloved hand method, were used to study the efficacy of three extenders viz.: GEPS, Modena and Androheps for preservation of boar semen at liquid state. The ejaculates were split into three parts and extended in GEPS (Glucose-sodium salt of EDTA-Potassium sodium tartrate-Sodium citrate dehydrate), Modena and Androheps extenders for preservation upto 168 hours at 15⁰C and evaluated per cent sperm motility, live sperm and live intact acrosome at 24 hours of interval. The mean sperm motility live sperm and live intact acrosome differed significantly (P<0.01) between extenders and between preservation periods. The interaction between extender and preservation period was found to be significant (P<0.05) only for sperm motility. A total of 90 female pigs (thirty for each extender) were inseminated with a dose of 80 ml (20 ml semen + 60 ml extender) of semen preserved for 72 to 120 hours at 15⁰C with GEPS, Modena and Androheps extenders. The percentage of conception rates were 81.8 in gilts and 84.2 in sows, 64.2 in gilts and 68.76 in sows and 84.62 in gilts and 82.36 in sows for the three extenders respectively. GEPS and Androheps were found to be superior to Modena based on sperm motility, live sperm, live intact acrosome and conception rate (on non return basis). Thirty apparently normal and healthy cyclic Hampshire Crossbred gilts and sows (aged 10 months to 2 years) maintained at villages near to NRC on Pig Rani, Guwahati were used for the synchronization of oestrus by feeding 10 mg progesterone orally daily for 7 days in empty stomach. Animals at synchronized oestrus were artificially inseminated with semen extended in GEPS extender irrespective of preservation periods. The percentage of synchronized oestrus were 57.14 in gilts and 62.50 in sows. The mean interval from treatment to onset of synchronized oestrus was 7.36 ± 0.78 days in gilts and 8.40 ± 0.37 days in sows. The difference having significant (P<0.05).The mean duration of oestrus was 78 ± 2.98 and 85.20 ± 3.32 hours in synchronized gilts and sows respectively, the difference being not significant. Most frequent behavioural signs of synchronized oestrus were nervousness, frequent grunting, seeking male, stance reflex and inappetance; the frequencies were 100.00, 75.00, 75.00, 62.50 and 62.51 per cent respectively in gilts and 100.00, 80.00, 80.00, 70.00 and 60.00 per cent respectively in sows. Swelling of vulva and pinkish vulva were the most common physical signs of synchronized oestrus exhibited and the intensity of signs was more in gilts than in sows. The conception rate in gilts and sows was 87.50 and 80.00 per cent respectively.