Thumbnail Image

Theses

Browse

20161
Reset filters
Subject: Veterinary Surgery and Radiology × Author: PRADHAN, EKTA × Start date: 2016 ×
Reset filters

Search Results

Now showing 1 - 1 of 1
  • Thumbnail Image
    ThesisItemOpen Access
    COMPARATIVE EVALUATION OF NANOSILVER, UNPASTEURIZED HONEY AND TURMERIC IN THE TREATMENT OF FULL THICKNESS CONTAMINATED CUTANEOUS WOUND IN RABBITS
    (Assam Agricultural University, Khanapara, Guwahati, 2016-07) PRADHAN, EKTA; Sarma, K. K.
    The present study was carried out on experimentally created full-thickness contaminated cutaneous wounds in New Zealand White rabbits with an aim to evaluate the healing efficacy of nanosilver, unpasteurized honey and turmeric. The experiment was carried out on 15 apparently healthy New Zealand White rabbits of either sex, divided into 3 equal groups. Full-thickness cutaneous wounds of 4x4 cm were created on the thoraco-lumbar region on either sides of the vertebral column under general anaesthesia and were left unattended for 48 hours to render it contaminated. Group A, Group B and Group C were subjected to ‘Silverkind Nanofine’ (nanosilver), unpasteurized honey and turmeric paste respectively. The rate of wound healing and macroscopic changes were recorded on the 3rd, 7th, 10th, 15th and 20th days of post wound creation. The bacteriological and biochemical investigation were performed on the 3rd, 5th, 7th and 10th days of post-wound creation and the histopathological investigations on the 3rd, 10th and 28th days of experiment. The rate of wound healing was found to be the fastest in nanosilver treated group, followed by the ones with unpasteurized honey and turmeric till the 28th day of experiment. Various macroscopic changes were observed during the course of treatment viz. peripheral swelling, serous exudation, dry casts over the wound, proliferating pinkish granulation tissue and fast contracting wound edges with re-epithelialization. However, clinically complete wound healing was observed on the 20th day of post-wound creation in Group A. The bacteriological investigation revealed presence of coagulase positive Staphylococcus spp., coagulase negative Staphylococcus spp. and Escherichia coli at the initial stages and eventually became a sterile wound by the 10th day of post-wound creation in both nanosilver based ointment and unpasteurized honey. Higher levels of total protein and carbohydrate were observed in Group B and Group C while the enzymatic investigations revealed decreasing levels of creatine kinase and aspartate aminotransferase which was more significant in Group A (P<0.01). The histopathological studies on the 28th day of post-wound creation revealed clinically complete healing in Group A with regeneration of all the epidermal layers and associated adnexa and better reorganization of the thick collagen bundles. From the above findings, it could be drawn that use of nanosilver based ointment was found to more effective in terms of wound healing of contaminated cutaneous wounds compared to the other two treatment regimes.