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20192
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Subject: Veterinary Pharmacology and Toxicology × Author: LALMUANTHANGA, C. × Start date: 2010 ×
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    ThesisItemOpen Access
    THERAPEUTIC EFFICACY AND ROLE OF CYTOKINES ON WOUND HEALING IN RATS BY SELECTED MEDICINAL PLANTS OF MIZORAM
    (College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2019-07) LALMUANTHANGA, C.; ROY, D.C.
    The present study was undertaken to evaluate the potential effect of methanol, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in three different models of wound in rats, incision, burn, diabetic excision wound models and also the polyherbal formulation of the plants at three different combination in diabetic wound model in rats. The cytokine, IL- 6, TNF-α and IL-10, blood biochemical profile, histopathological examination of tissue, antimicrobial effect of different extracts of plant under study and the analysis of the antioxidant property of the plants were undertaken to find the correlation with the wound healing property of plant extracts. The yields of methanol extract of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria were 166.93gm (16.693%), 78.00 (7.80%), 60.00 (6.00%), 117.00 (11.70%) and 50.68 (5.068%) per 1000 gram of dry powder respectively. The preliminary analysis of phytochemical constituent of plant shows that Parkia timoriana extract was found to contain tannins, saponins, flavonoids, terpenoids and alkaloids. Securinega virosa extract was found to contain tannins, flavonoids, terpenoids and alkaloids. Abelmoschus moschatus extract was found to contain tannins, flavonoids, terpenoids; alkaloids and phlobotannins. Scoparia dulcis extract was found to contain tannins, saponins, flavonoids, terpenoids and alkaloids and Blumea lanceolaria extract was found to contain flavonoids, phlobotannins, terpenoids, alkaloids and tannins. All the plant extracts were found to be safe @ 2000 mg/kg after testing the acute oral toxicity in rats and mice. Topical administration of methanol, chloroform and ether extract of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in incision wound model in rats @ 10 and 20 % w/w ointment increased the breaking strength of wound when compared with control and standard treated groups at P<0.001 significant level. An increasing trend in the total protein, albumin and globulin level of wound tissue was observed with some variation between control and the treated groups. The level of AST, ALT and glucose in the treatment groups were significantly lower than the control group at P<0.05 and P<0.00. The percent of wound contraction after topical application of 10 and 20% w/w ointments of methanol, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in burn wound model in rats were significantly higher (P<0.001) on day 4, 8, 12 and 16 as compared to ii control group and standard group; but individual variation for significant different with standard drug treated group occurs amongst the plant extracts. There were remarkable fibroblast collective tissue proliferation, collagenation, mononuclear infiltration, angiogenesis, vascular engorgement and epithelialisation on histopathological examination. In excision diabetic wound model in rats, the methanolic, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria administered @ 250 and 500mg/kg P.O. showed significantly increased (P<0.05; P<0.01 and P<0.001) percent of wound contraction on day 4, 8, 12 and day 16 as compare to the control group and the epithelisation time were significantly reduced. Regenerating skin appendages tissue proliferation, mononuclear infiltration which confirm the healing action which are quite comparable to the standard (Vitamin E) treated group. Biochemical analysis revealed that the blood glucose, protein, globulin and albumin levels in control groups are significantly higher in most of the cases and AST, ALT and creatine kinase are significant reduced at P<0.05; P<0.01 and P<0.001. The cytokine analysis of blood plasma on day 1 and day 9 of post wound creation and after treatment with plant extracts shows that the level of IL-6 and TNF-α were decreasing on day 9 as compared to the previous sampling. The different extracts of plants at two different dosing @ 10 and 20 % w/w ointment showed the significance difference (P<0.05; P<0.01 and P<0.001) between the groups. Contrary to the level of IL-6 and TNF- α, the level of IL-10 was increased doubled fold or more on day 9 in all the treated groups. The topical application of Poly I MZ, Poly II MZ and Poly III MZ @ 20% w/w ointment each on diabetic incision wound showed significantly increased the breaking strength when compared to control group.
  • Thumbnail Image
    ThesisItemOpen Access
    THERAPEUTIC EFFICACY AND ROLE OF CYTOKINES ON WOUND HEALING IN RATS BY SELECTED MEDICINAL PLANTS OF MIZORAM
    (College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2019-07) LALMUANTHANGA, C.; ROY, D. C.
    The present study was undertaken to evaluate the potential effect of methanol, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in three different models of wound in rats, incision, burn, diabetic excision wound models and also the polyherbal formulation of the plants at three different combination in diabetic wound model in rats. The cytokine, IL- 6, TNF-α and IL-10, blood biochemical profile, histopathological examination of tissue, antimicrobial effect of different extracts of plant under study and the analysis of the antioxidant property of the plants were undertaken to find the correlation with the wound healing property of plant extracts. The yields of methanol extract of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria were 166.93gm (16.693%), 78.00 (7.80%), 60.00 (6.00%), 117.00 (11.70%) and 50.68 (5.068%) per 1000 gram of dry powder respectively. The preliminary analysis of phytochemical constituent of plant shows that Parkia timoriana extract was found to contain tannins, saponins, flavonoids, terpenoids and alkaloids. Securinega virosa extract was found to contain tannins, flavonoids, terpenoids and alkaloids. Abelmoschus moschatus extract was found to contain tannins, flavonoids, terpenoids; alkaloids and phlobotannins. Scoparia dulcis extract was found to contain tannins, saponins, flavonoids, terpenoids and alkaloids and Blumea lanceolaria extract was found to contain flavonoids, phlobotannins, terpenoids, alkaloids and tannins. All the plant extracts were found to be safe @ 2000 mg/kg after testing the acute oral toxicity in rats and mice. Topical administration of methanol, chloroform and ether extract of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in incision wound model in rats @ 10 and 20 % w/w ointment increased the breaking strength of wound when compared with control and standard treated groups at P<0.001 significant level. An increasing trend in the total protein, albumin and globulin level of wound tissue was observed with some variation between control and the treated groups. The level of AST, ALT and glucose in the treatment groups were significantly lower than the control group at P<0.05 and P<0.00. The percent of wound contraction after topical application of 10 and 20% w/w ointments of methanol, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria in burn wound model in rats were significantly higher (P<0.001) on day 4, 8, 12 and 16 as compared to ii control group and standard group; but individual variation for significant different with standard drug treated group occurs amongst the plant extracts. There were remarkable fibroblast collective tissue proliferation, collagenation, mononuclear infiltration, angiogenesis, vascular engorgement and epithelialisation on histopathological examination. In excision diabetic wound model in rats, the methanolic, chloroform and ether extracts of Parkia timoriana, Securinega virosa, Abelmoschus moschatus, Scoparia dulcis and Blumea lanceolaria administered @ 250 and 500mg/kg P.O. showed significantly increased (P<0.05; P<0.01 and P<0.001) percent of wound contraction on day 4, 8, 12 and day 16 as compare to the control group and the epithelisation time were significantly reduced. Regenerating skin appendages tissue proliferation, mononuclear infiltration which confirm the healing action which are quite comparable to the standard (Vitamin E) treated group. Biochemical analysis revealed that the blood glucose, protein, globulin and albumin levels in control groups are significantly higher in most of the cases and AST, ALT and creatine kinase are significant reduced at P<0.05; P<0.01 and P<0.001. The cytokine analysis of blood plasma on day 1 and day 9 of post wound creation and after treatment with plant extracts shows that the level of IL-6 and TNF-α were decreasing on day 9 as compared to the previous sampling. The different extracts of plants at two different dosing @ 10 and 20 % w/w ointment showed the significance difference (P<0.05; P<0.01 and P<0.001) between the groups. Contrary to the level of IL-6 and TNF- α, the level of IL-10 was increased doubled fold or more on day 9 in all the treated groups. The topical application of Poly I MZ, Poly II MZ and Poly III MZ @ 20% w/w ointment each on diabetic incision wound showed significantly increased the breaking strength when compared to control group.