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ThesisItem Open Access INVESTIGATION ON PARASITES OF DUCK IN UPPER ASSAM WITH SPECIAL REFERENCE TO HAEMOPROTOZOA(College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-781022, 2017-07) Borah, Nanswita; Phukan, Sulekha ChoudhuryA survey was conducted in seven districts of upper Assam for a period of one year from June, 2016 to May, 2017 to study the prevalence of parasites of domestic ducks in upper Assam. Out of a total of 675 numbers of ducks examined, 467 ducks were found positive for different ectoparasites showing the overall prevalence of 69.19 per cent. Ducks from Gaurisagar of Sibsagar district had highest 92.86 per cent and Lahowal of Dibrugarh district had lowest 58.82 per cent prevalence for ectoparasites. Five different lice and two different types mites were recovered from different body parts of duck, of which infestation with Lipeurus caponis (13.33%) was highest followed by Menopon gallinae (12.74%), Menacanthus stramineus (10.67%), Columbicola columbae (8.44%), different feather mites viz. Dubininia melopsittaci, Megninia ginglymura and Bdellorhynchus sp. (5.19%), Goniodes sp. (3.70%) and larva of Trombiculid mite (0.89%), respectively. The three feather mites, larva of the Trombiculid mite and Columbicola columbae were first time reported from Assam in duck. Examination of 724 faecal samples collected from different localities of upper Assam revealed 333 samples to be positive for helminthes with overall prevalence of 45.99 per cent. Out of the positive samples, 314 samples were positive for single and 19 for mixed infection with incidence of 94.29 per cent and 5.71 per cent. Samples from Gaurisagar of Sibsagar district had highest 68.96 per cent of helminthic infection. Helminthic infections of ducks were not recorded from samples collected from Baghchung of Jorhat district and Joysagar of Sigsagar district. Faecal samples collected from ducks reared under free range system had highest prevalence of 57.84 per cent for helminthes followed by semi intensive system of rearing (35.34%) and no helminthic infections were recorded from faecal samples of ducks reared under intensive system. Season wise prevalence of helminths in ducks on the basis of faecal examination revealed highest during pre monsoon (73.56%) and lowest during winter (20.92%). Out of 724 faecal samples examined, 20 samples were positive for Coccidia (Isospora sp.) with the overall prevalence of 2.76 per cent. Post mortem examination of 774 carcasses of ducks from different localities of different districts in upper Assam revealed 518 carcasses to be positive for helminthic infections. The overall prevalence of helminthes was recorded as 66.93 per cent. 232 carcasses were found positive for cestodes (44.79%), 241 for trematodes (46.52%) and 45 for nematodes (8.69%). Carcasses from Demow of Sibsagar district had highest prevalence of 86.05 per cent and lowest from Kakopathar of Tinsukia district with 50.00 per cent prevalence for helminthes. Post mortem examination of ducks reared under free range system revealed highest 78.21 per cent for helminthes followed by semi intensive system of rearing (70.33%). Helminthic infection was not recorded from ducks reared under intensive system. Seasonal prevalence of helminthes on the basis of post mortem examination revealed highest during monsoon (72.28%) and lowest during winter season (51.43%). On the basis of morphological studies, 7 different species of cestodes and 1 larval stage of cestode, 8 different species of trematodes and 5 different species of nematodes were identified and recorded. Highest incidence of cestodes was recorded with Hymenolepis collaris (35.78%) followed by Hymenolepis carioca (35.34%), Fimbriaria fasciolaris (22.41%), Hymenolepis lanceolata (1.74%), Raillietina tetragona (1.29%), Raillietina echinobothridia (1.29%) and Raillietina cesticillus (1.29%) and lowest with plerocercoid (0.86%). Highest incidence of trematodes was recorded with Echinostoma revolutum (24.07%), Hypoderaeum sp. (18.67%), Psilorchis sp. (18.67%), Echinostoma paraulum (16.60%), Paryphostomum sp. (10.79%), Prosthogonimus sp. (8.30%), Tracheophilus cymbius (2.07%) and Psilochasmus sp. (0.83%). Highest incidence of nematodes was recorded with Tetrameres sp. (57.78%), Heterakis gallinarum (17.78%), Heterakis dispar (17.78%), Strongyloides avium (4.44%) and Porrocaecum sp. (2.22%). Hymenolepis carioca, Hymenolepis lanceolata, Raillietina tetragona, Hypoderaeum sp., Echinostoma paraulum, Prosthogonimus sp., Psilochasmus sp. and Heterakis dispar were first time reported from Assam in duck. Seasonal prevalence of helminthes according to breeds of duck revealed highest prevalence of helminthes in monsoon season (82.54%) and lowest in winter (26.67%) from Patihanh, highest in pre monsoon (85.26%) and lowest in monsoon (54.29%) from Khaki Campbell, highest in pre monsoon season (85.37%) and lowest in winter (56.52%) from Chara ducks and prevalence of helminthes in Muscovy ducks during monsoon season was 80 per cent. Overall prevalence of helminthes was recorded highest in adults (76.73%) and lowest in young ducks (54.41%). Seasonal prevalence of helminthes according to age of ducks revealed highest prevalence of helminthic infection during pre monsoon (67.83%) and lowest during winter season (34.88%) in youngs and highest prevalence during post monsoon season (85.29%) and lowest during winter season (61.29%) in adults. Overall prevalence was recorded highest in female (67.44%) and lowest in male (66.41%). Seasonal prevalence of helminthes according to sex revealed that males had highest helminthic infection during monsoon (80.41%) and lowest during winter season (43.14%). Similarly, females had highest during pre monsoon season (73.88%) and lowest during winter season (57.41%). Prevalence of haemoprotozoa in ducks revealed that, out of 300 thin blood smears prepared, only 4 showed the presence of Haemoproteus sp. infection in RBC. The overall prevalence of infection was 1.33 per cent. Haematological changes observed due to helminthes and haemoprotozoan parasites of duck revealed decrease in Haemoglobin (9.38±0.13), PCV (27.98±0.36) and TEC (1.69±0.08) with increase in TLC (43.18±1.76) in infected ducks. On post mortem examination, various pathological lesions were found associated with Hymenolepis sp. and Echinostoma sp. in intestines and Tetrameres sp. in proventricles of ducks. Intestines of duck showed catarrhal enteritis and congestion in the mucosal surface of the small intestine for Hymenolepis sp. and haemorrhages and catarrhal enteritis for Echinostoma sp. Microscopic examination revealed marked thickening of intestinal villi with infiltration of inflammatory cells. Some villi showed haemorrhagic patches, degeneration and necrosis. Desquamated epithelial lining of intestinal villi were found to be deposited in the lumen of the intestine. Cross section of Hymenolepid parasite was found attached to the superficial epithelium surrounded by infiltrating cells. Gross examination of infected proventiculus revealed small blackish elevated spots visible from the luminal surface. Microscopic examination revealed cut sections of female parasite inside the proventricular gland. Glandular epithelium surrounding the parasite exhibited pressure atrophy and the lumen of the gland was found dilated. Tunica muscularis around the glands was found thickened and inter glandular spaces was found enlarged and thickened with proliferation of fibrous connective tissue and was found infiltrated with monocytes and polymorphs. The genera Echinostoma sp. (Identified as Echinostoma revolutum on the basis of morphological characteristics) and Hypoderaeum sp. were identified as Echinostoma friedi and Hypoderaeum conoideum on the basis of ITS2 sequence identity. The present isolate of Echinostoma sp. showed 100 % identity with Echinostoma friedi the isolate of Hypoderaeum sp. showed 99% identity with Hypoderaeum conoideum The Molecular characterization of either Echinostoma friedi or Hypoderaeum conoideum could not be accomplished due to unavailability of more than one ITS2 sequence of these species in NCBI database. Probably it might be the first report of Echinostoma friedi in India. The ITS2 sequence based species identification of Psilorchis sp. could not be accomplished due to absence of Psilorchis ITS2 sequence in NCBI database. This might probably be the first report of ITS2 sequencing of Psilorchis sp. either in India and abroad.