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20161
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Subject: Veterinary Epidemiology and Preventive Medicine × End date: 2016 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    OUTER MEMBRANE VESICLE (OMV) OF Pasteurella multocida AS POTENTIAL IMMUNOGEN FOR PIG
    (Assam Agricultural University, Khanapara, Guwahati, 2016-07) MUKHERJEE, SHYAMANANDA; Mahato, G.
    The present study was undertaken to evaluate the immunogenic potential of Outer Membrane Vesicles (OMVs) and bacterin vaccine and their comparison. Extractions were made from a highly pathogenic pig isolate of Pasteurella multocida of capsular type A. For the study, isolation of P. multocida was attempted from apparently healthy, ailing and dead pigs. Following screening of the 171 field samples collected from various places, 17 (9.94 %) were found to be positive for P. multocida. Fourteen of the 17 isolates subjected to PCR targeting species-specific gene revealed a distinct band of 460 bp, which was a confirmation of the isolates as P. multocida. Among the confirmed P. multocida isolates, nine were identified as capsular type A (1044 bp), while the remaining five were capsular type D (657 bp), based on multiplex cap-PCR results, targeting hyaD-hyaC and dcbF genes, respectively. Both OMVs and bacterin were extracted from the most pathogenic porcine isolate of P. multocida capsular type A selected on the basis of pathogenicity trial in mice. Vaccines were prepared from the OMVs extract and bacterin of P. multocida and immunized to pigs. Following immunization with vaccines prepared from both the extracted OMVs and bacterin in natural host, pig through intramuscular (i/m) route, a gradual increase in the mean serum antibody titre was observed in both the immunized groups. The vaccine prepared from the OMVs extract showed peak antibody titre on 60th day (6.40± 0.19) post-vaccination. On the other hand, the vaccine preparation with the bacterin extract showed peak titre on 28th day (6.10± 0.14) of post-immunization and no significant difference was observed between the vaccine preparations throughout the study period (upto 90 days). Based on the statistical analysis of the mean serum antibody titre at different days post-immunization with the two preparations, no significant difference was observed between the two vaccine preparations of P. multocida. The post vaccinated serum samples collected on different days were subjected for protection test in mice. The result of passive mouse protection test revealed that the 21st and 28th day post vaccination serum sample (OMV vaccine) and 28th day serum sample (bacterin vaccine) produced (100%) protection in mice.