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20181
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Subject: Plant Pathology × Author: BARUAH, KOLLOL PRATIM × End date: 2018 ×
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    ThesisItemOpen Access
    BIOENGINEERING MICROBIAL ANTAGONISTS WITH JATROPHA (Jatropha curcas) OIL AND RESULTANT SUPPRESSION OF BACTERIAL WILT (Ralstonia solanacearum) OF TOMATO
    (AAU, Jorhat, 2018) BARUAH, KOLLOL PRATIM; Bora, L. C.
    Tomato (Solanum lycopersicum L.) is one of the most important “protective foods” both because of its special nutritive value and widespread production. Bacterial wilt of tomato caused by Ralstonia solanacearum is one of the major disease causing a loss up to an extent of 100 per cent. The use of bio-control agents against vegetable diseases are reported to be quite effective and inexpensive. The efficiency of bio-control agents could be enhanced to a great extent when integrated with a known amount of phyto-extract. The efficacy of Jatropha oil (Jatropha curcas) was evaluated in the present study for suppression of the bacterial wilt pathogen, R. solanacearum The in-vitro studies revealed that Jatropha oil @ 5000 ppm, 10000 ppm & 50000 ppm could suppress the pathogen up to an extent of 25.3, 64.0 and 66.2 per cent respectively. Bioengineering Jatropha oil with five microbial antagonists, viz., Pseudomonas fluorescens, Bacillus thuringiensis, Beauveria bassiana, Metarhizium anisopliae and Trichoderma viride revealed a positive interaction. Amongst these, T. viride was found most compatible followed by P. fluorescens, B. thuringiensis, B. bassiana and M. anisopliae. The in vivo studies revealed that P. fluorescens along with Jatropha oil could suppress bacterial wilt of tomato significantly (80%) followed by T. viride + Jatropha oil (60%); B. thuringiensis + Jatropha oil (40 %) and B. bassiana + Jatropha oil (40%). Combination of these bio agents and Jatropha oil also showed better yield of R. solanacearum inoculated tomato plants when compared to application of bio agents alone. Highest yield was recorded in the treatment combination of P. fluorescens + Jatropha oil (315.8 g/plant) followed by T. viride + Jatropha oil (230.1 g/plant), B. thuruingiensis + Jatropha oil (228.58 g/plant) and B. bassiana + Jatropha oil. (171.92 g/plant) Similarly, the yield attributing characters of tomato treated with bio agents and Jatropha oil was found better as compared to application of bio agents alone.