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Subject: Nematology × Author: Arandhara, Babyshree × Start date: 2020 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    EVALUATION OF DAMAGE CAUSED BY ROOT KNOT NEMATODE AND ITS SURVIVAL IN TUBEROSE DURING STORAGE OF BULB
    (2023) Arandhara, Babyshree; Gogoi, B. B.
    In the present investigation on evaluation of damage caused by root knot nematode and its survival in tuberose during storage of bulbs, it was observed that all growth parameters of tuberose were affected with corresponding increase in inoculum levels. Plant showed gradual increase in number of galls, egg masses and final nematode population in the soil with increase in population levels of root knot nematode from 20 to 10,000 nematode per pot. Reproductive rate was found to be maximum at lowest inoculum and minimum at highest inoculum of 10,000 M. incognita per kg soil. In the investigation on survival of root knot nematode during storage of bulb, the study of stored planting material (bulbs of tuberose) reveled that all the stages of root knot nematode were present within the bulbs. J2 were found within the bulb and also in between the layers of bulbs. J3, J4 and adult females were found in the bulb tissue. Some adults were also found to be aggregate in between the layers of bulb tissue. There was a reduction in nematode population (56.91%) during the storage period of 6 months. Egg masses within the bulb were brownish in colours, protected by gelatinous matrix and the bulb tissue. The egg masses attached to the basal portion of the bulbs were deep orange colour. The change in colour of egg masses attached to the basal portion of the bulbs during the storage period might be due to drying and tanning of the gelatinous matrix may be due to low moisture for an extended period of time. Germination percentage of the stored bulb was recorded to be 70%. There was 30% reduction in germination of stored bulb due to heavy nematode infestation and drying of bulbs in storage. When infected stored bulbs were replanted in pots with sterilized soil, survival and transmission of nematode to the new crop was confirmed by the developed symptoms (gall and egg mass formation) on roots.