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  • ThesisItemOpen Access
    PHYSIOLOGICAL PERFORMANCE OF RAPESEED (Brassica rapa var. Toria) GENOTYPES UNDER RAINFED CONDITION OF ASSAM
    (2023) CHINTEY, RAJESH; Goswami, Ratna Kinkor
    The present research work entitled “Physiological performance of rapeseed (Brassica rapa, var. Toria) genotypes under rainfed condition of Assam” was carried out at the Instructional-cum-Research (ICR) Farm, Assam Agricultural University, Jorhat-13, Assam during rabi seasons of the years 2021-22 and 2022-23. The main objectives of the study were to find out the suitable superior genotypes based on the physiological efficiency and to identify the parameters which contribute significantly towards yield improvement in rapeseed. The field experiment was laid out in randomized block design with three replications comprising of 22 different genotypes of rapeseed including TS-38 (Check), TS-46, TS-67, TS-36, TS-29, TS-75-1, TS-75-1TL, TS-75-2ME, TS-75-2-MM, TS-76-1, TS-76-2, JT-90-1, Panchali, Bhawani, CG Toria-4, TKM-20-1, TKM-20-2, JT-14-5, PT-2018-09, CG Toria-3, Tapeshwari and PT-303. The crops were grown following the recommended package of practice. All the morpho-physiological, biochemical, quality parameters, yield attributes and yield of the crops were taken following the standard methodologies. The results of the study indicated a significant variation of all the morpho-physiological, phenological, quality parameters, yield attributes and yield among the genotypes. The genotypes, TS-75-2ME, TS-38, PT-303 and TS-36 exhibited morpho-physiological superiority with regard to more number of branches, stem diameter, accumulation of more photosynthates, more AGR, CGR and leaf chlorophyll. The same genotypes also recorded significantly higher yield attributes and seed yield. The correlation studies also showed a significant positive correlation of these parameters with seed yield in the set of the genotypes, indicating clearly that these parameters may be regarded as morpho-physiological indices for improving crop productivity in rapeseed. From the results, the genotypes, TS-75-2ME, TS-38, PT-303 and TS-36 may be regarded as superior genotypes suitable for growing under rainfed condition during rabi season in Assam and North-Eastern states of India and may be used as genetic sources in further yield improvement programme of rapeseed.
  • ThesisItemOpen Access
    STUDY ON DIVERSITY OF RICE IN NAGALAND AND THEIR SUITABILITY TO CHANGING CLIMATE
    (AAU, Jorhat, 2022) Merentoshi; Das, Ranjan
    The present investigation was carried out during the year 2018 to 2020 to evaluate the performance of some rice (Oryza sativa L.) genotypes from Nagaland under different CO2 and temperature regimes viz., Treatment I = Field (control), treatment II = (CO2 (550±20ppm) + Temp. of 40 C > ambient) and Treatment III = CO2 (750±20ppm) + Temp. of 60 C > ambient) under fully automated bioreactors with CO2 and temperature control facilities in Stress Physiology Laboratory, Department of Crop Physiology, Assam Agricultural University, Jorhat-13. In the first experiment, 75 rice genotypes collected from different locations of Nagaland were subjected for initial screening for tolerance against elevated CO2 and temperature out of which 7 genotypes were selected for further study on their response mechanism of tolerance by comparing with the national check variety N22. The results revealed that treatment II had a positive impact on the various morpho-physiological parameter viz., plant height, number of leaves, leaf area index, root volume and length, membrane stability index, total leaf chlorophyll content, leaf nitrate reductase activity, proline in all the genotypes whereas a declining trend indicating the deleterious effect of high temperature, at a higher CO2 and temperature in treatment III was observed in all these parameters. Similarly, significant variation in photosynthesis and its related parameters (viz. stomatal conductance, internal CO2, transpiration) affected the photosynthetic rate and their partitioning to root, shoot and reproductive organ under both the treatments as compared to field condition. These factors ultimately contributed to growth efficiency and yield of the plant. Amongst the treatments, significant increase of H2O2, MDA content was recorded in treatment III as compared to treatment III. Kohima Special and N22 recorded lower H2O2 and MDA when compared to other local genotypes. Hence these two genotypes could maintain their plant water status as evidenced by their higher MSI under both the treatments. The maintenance of higher plant water status in Kohima Special and N22 could be attributed to their higher RLWC and increased compatible solutes viz. proline content and non structural carbohydrate contents. Further, studies in tolerance indices indicated that among the tested local genotypes, Kohima Special and Lisem were tolerant whereas Tatza and Tzumma showed to be most susceptible under high temperature. In the molecular analysis, using SCoT molecular markers out of 77 bands amplified, 55 were found to be polymorphic with 68.99 per cent polymorphism. Only four primers SCoT28 and SCoT34 not found to be polymorphic alleles, while remaining 23 SCoT primers showed both polymorphic alleles. Yield and yield attributing characters are governed by all the morphological, physiological and biochemical processes. Among yield parameters, 1000-grain weight, spikelets per panicle and total grain weight per plant were significantly higher due to elevated CO2 at treatment II since the genotypes could maintain a higher photosynthetic rate and had a superior plant defence mechanisms but were significantly decreased under high temperature at treatment III which was negatively influenced by all the processes resulting in decrease in yield and yield attributing characters in all the genotypes.
  • ThesisItemOpen Access
    PERFORMANCE OF SOME BANANA GERMPLASM UNDER MOISTURE STRESS CONDITION AND ITS AMELIORATION THROUGH CHEMICAL INTERVENTION
    (AAU, Jorhat, 2020-07) Saikia, Amarjit; Kalita, Prakash
    A study was conducted at the Experimental Farm, Department of Horticulture and laboratories of Department of Crop Physiology, Assam Agricultural University, Jorhat during the period 2017-2019 with twenty nine germplasm of banana viz., Athiya (BB), Manohar (ABB), Dwarf Jahaji (AAA), Barjahaji (AAA), Ketchulepa (ABB), Jatikal (ABB), Kachkal (white) (ABB), Kachkal (green) (ABB), Honda (AAB), Gobin Tulchi (AAB), Doodhsagar (AAB), Digjowa (AAB), Chenichampa (AAB), Malbhog (AAB), Amrit Sagar (AAA), Man Jahaji (AAA), Agnisagar (AAA), Bhimkal (BB), Lesari Manohar (ABB), Simolu Manohar (ABB), Suti Jahaji (AAA), Bhat Manohar (ABB), Sahabhal (AAA), Fesa manohar (ABB), Dwarf Cavendish (AAA), Grand Naine (AAA), Bogimonohar (ABB), Odil (AAA) and Thengransu (ABB) to assess their performance under moisture deficit condition that prevails naturally during November to January every year in Assam due to lack of rainfall. The germplasm were also evaluated for any change in their tolerance level towards soil moisture deficit after receiving certain chemicals viz. maltose and trehalose through foliar spray. The experiment was laid out in Randomized Block Design (RBD) with twenty nine germplasm. The number of replications was five. The observations on various parameters were recorded on 3rd, 5th, 7th and 9th MAP in the main crop and one month after application of chemicals in the case of ratoon crop. The mean monthly temperature during the crop growing season ranged from 10.8°C to 29.8°C, the rainfall ranged from 0.0-42.7 mm and the relative humidity ranged between 55-97 per cent. The soil of the experimental field was acidic, well drained and sandy loam in texture with low available N and K and medium available P content. Moisture stress progressively reduced the values of relative leaf water content, leaf area, leaf area index, chlorophyll content (a, b, & total) from 3rd to 9th months after planting; while the contents of proline, total soluble protein, free amino acid and level of lipid peroxidation in leaf tissue increased with increase in the magnitude of moisture deficit during that period. Germplasm viz., Barjahaji, Bhimkal, Athiya etc. recorded higher values for various physiological and biochemical parameters namely relative leaf water content, proline content, total soluble protein content, free amino acid content, leaf area, leaf area index, leaf chlorophyll content, number of functional leaves etc. especially during the period when the soil moisture content was very low. Observed higher efficiency in terms of physiological and biochemical parameters in these germplasm might have contributed immensely 6 towards realisation of better yield attributing characters like fruit length, girth of finger, volume of finger weight of finger, weight of second hand, number of hands per bunch, number of fingers per hand and bunch weight under Assam condition which faces severe shortage in soil moisture (soil moisture content was only 33.70 per cent of the field capacity at 7th MAP) under rainfed situation during the months of winter mostly coinciding the reproductive stage of the crop. The germplasm Barjahaji was found to be the highest yielder followed by the germplasm Bhimkal and Athiya, whereas Gobin Tulchi was found to be the lowest performers. It may be concluded that the germplasm Barjahaji, Bhimkal and Athiya are physiologically more tolerant to moisture shortage. Application of chemials (trehalose and maltose) could positively influenced various adaptive mechanism during water deficit condition. The characters like relative leaf water content, proline content, total soluble protein content, free amino acid content, chlorophyll content, leaf area, leaf area index etc. did show positive and significant correlation with bunch yield per plant. On an average with foliar application of maltose (60 mM), trehalose (60 mM) and maltose (60 mM) + trehalose (60 mM) during December, increased the yield by 1.18, 1.76 and 2.44 per cent, respectively over control (spray with distilled water). The highest benefit: cost ratio in case of dwarf germplasm, medium tall germplasm and tall germplasm were found to be 4.66 (Dwarf Jahaji), 4.39 (Barjahaji) and 4.41 (Athiya) respectively all with distilled water spray.
  • ThesisItemOpen Access
    TAPPING OF CARBON DIOXIDE IN RICE ECOSYSTEM THROUGH AZOLLA CULTIVATION
    (AAU, Jorhat, 2017-07) Khwairakpam, Rowndel; Das, Ranjan
    The present investigation was carried out in the stress physiology laboratory under fully automated bioreactor where tapping of CO2 and external injection regulation CO2 facility were available. To understand the assimilation and N accumulation in Azolla-Rice ecosystem in response elevated CO2 possible interactions of CO2 and Azolla on the growth and yield of rice investigation was conducted during the kharif season of 2014 and 2015.The studies revealed that there was variable response of photosynthetic parameters which lead to impact on carbon sequestration potential as well as change in O2 evolution under varying levels of CO2 environment under rice-Azolla ecosystem. In case of fluorescence and related parameters, there was deviation from the normal range as the CO2 concentration increased, however these deviations were minimized in presence of Azolla. These were also same in case of ETR, NPQ, PQ, ф PSII etc. Photosynthetic and its related parameters viz. gs, Ci, T, etc. were found to be related to stomatal density and size. These factors ultimately affected plant growth which was translated as yield. Elevated (500 ppm) CO2 with Azolla has a greater potential for C sequestration, while soil organic carbon enhancement helped increase crop productivity and sustainability which was evident from higher yield and yield attributing parameters. Soil microbial population with higher C: N ratio was recorded under elevated CO2 with rice-Azolla ecosystem. There have been increasing concerns regarding the role of soil microbial population in biological stabilization of SOC in agricultural soils because change in the soil microbial population within rhizo-spheres with changes in organic matter input. Significant reduction of NPK was recorded due to elevated CO2, however; reduction rate was variable with Azolla when compared with without Azolla and ambient. Conversion of inorganic nitrogen was significantly reduced in elevated CO2 however there was amelioration affect under 500 ppm CO2 with Azolla due to significant increase in key enzymes such NR, NiR and GS syntheses activity. CO2 enrichment decreased the N concentration in rice without any change in the C content, leading to an increase in C:N ratio However variability among systems also recorded. This study: (1) depicts the changes in microbial population and fungal predominance; (2) infers a biological stabilizing mechanism behind the C sequestration which includes physical-chemical stabilization as the major controlling processes leading to variation of yield. Azolla has enormous potential to sequester of atmospheric CO2 due to its rapid growth in freshwater without the need for a soil-based nitrogen source. Azolla is a novel opportunity to expand and diversify the supply of fertilizers and production of rice fields vis a vis tapping the CO2 and enhancing the O2 evolution system under changed environmental conditions.
  • ThesisItemOpen Access
    PHYSIOLOGICAL CHARACTERIZATION OF SOME WHEAT GENOTYPES FOR HIGHER NITROGEN USE EFFICIENCY AND YIELD POTENTIAL IN ASSAM
    (AAU, Jorhat, 2017-09) Chack, Sonbeer; Bharali, B.
    Wheat is one of the most important cereal crops globally, and is a staple food for about one-third of the world’s population (Hussain et al., 2002). Nitrogen is the most important yield-limiting nutrient for crop production in the world (Huber and Thompson, 2007). Two field experiments were conducted for two consecutive years near Stress Physiology Laboratory, Department of Crop Physiology, Assam Agricultural University. The main objective of the study was to characterise some wheat genotypes (collected from East Zone of India) physiologically for higher nitrogen use efficiency (NUE) and yield potential in Assam. In the first year experiment (November, 2014-April, 2015): Nitrogen @ 0, 50, 100, 150 kg ha-1 (as Urea) in the form of granules @ 50% of N as basal with the whole recommended doses of P (as Single super phosphate) and K (as Muriate of potash), and the rest 50% Urea were applied at maximum tillering stage. In the second year experiment (November, 2015-April, 2016), Nitrogen @ 0, 500, 1000, 1500 ppm (equivalent to granules in kg ha-1) were applied as foliar spray at the maximum tillering stage (once in a week for two weeks) of the crop. In the first year experiment, the varieties having the higher NUE in leaves were Wheat 231 (11.36%)> HP 1744 (8.58%)> Pusa Gold (8.42%), and higher NUE in seed were Wheat 231 (19.41%)> HP 1744 (10.01%)> Pusa Gold (9.29%)> PBW 343 (8.27%)> HD 2967 (6.08%). The physiological parameters contributing to the higher NUE in the varieties were maximum Leaf area in Wheat 231(16.81, 17.55 cm2 plant-1)> HD 2967 (15.11, 16.98 cm2 plant-1) at maximum tillering and PI stages respectively. The maximum leaf number was observed in wheat 231 (23.27)> HP 1744 (21.53)> PBW 343 (19.43) under the highest dose of N i.e. 150 kg ha-1 at panicle initiation stage. The other physiological parameters viz. root length of Wheat 231 (12.77 cm)>Pusa Gold (12.57 cm), root volume of Wheat 231 (10.31 cc)> PBW 343 (8.43 cc), and shoot dry weight of Wheat 231 (1.66 g)> HP 1744 (1.55 g) were responsive to augment NUE in the wheat varieties. The variety Wheat 231 (36.18, 34.26)> HP 1744 (33.19, 33.60) showed the highest NR activity (nmol NO2- g-1 fw of leaf) at both Maximum tillering and PI stages. Wheat 231 also exhibited higher N contents in leaf (0.41%) at PI stage, in grain (0.61%), and in leaf (0.37%) at harvest. The yield and yield attributes for higher NUE in the variety Wheat 231 were number of seed spike-1 (48.73), spike length (12.72 cm), spike weight (4.01 g), test weight (45.67), economical yield (3.02 t ha-1) and harvest index (47.32%). Nitrogen @ 150 kg ha-1 imparted higher NUE in leaves (12.48%) and grain (15.79%). In this contest, the physiological & biochemical parameters were leaf number (22.45), leaf area (18.30 cm2), root volume (8.41cc), NR activity (34.08 nmol NO2- g-1 fw of leaf) at PI stage, and starch content in leaf (271.05 mg g-1 d.w.) at maximum tillering stage, total N uptake (37.50%) and N content in seed & leaf (0.46%, 0.38%) at harvest as compared to values in controlled one. In the second year experiment, the varieties having the higher NUE in leaves were of Wheat 231 (10.90%)> HP 1744 (8.15%); and in case of seed, it was in Wheat 231 (18.74%) only. The physiological parameters contributing to the higher NUE in the variety Wheat 231 were: maximum leaf area (15.29 cm2 plant-1) at PI stage; root length (14.66 cm) at PI stage, NR activity (37.40 nmol NO2- g-1 fw hr-1) at maximum tillering stage, nitrogen content in leaf (0.456%) at PI stage and starch content in leaf (221.20, 241.22 mg g-1 d.w) at both maximum tillering and PI stages. The yield and yield attributes for higher NUE in the variety Wheat 231 were number of seed spike-1 (46.39), spike length (12.03 cm), test weight (44.00 g), economical yield (2.76 t ha-1) and harvest index (47.07%). The N dose @ 1500 ppm triggered higher NUE in leaves (12.07%) and grains (15.61%) at harvest. In this case, the other physiological & biochemical parameters contributing the higher NUE were leaf area (16.05 cm2), root length (14.39 cm), NR activity (19.08-37.69 nmol NO2- g-1 fw hr-1), N content in seed & leaf (0.493% & 0.394%) at harvest and nitrogen harvest index (0.56%) as compared to values in the controlled one. Overall, in the experiment 1, the variety Wheat 231 possessed the highest score (12) followed by PBW 343 (3)> Pusa Gold (1), and the variety HP 1744 & HD 2967 obtained zero score at harvest. In the second year, too, Wheat 231 (11) scored the highest followed by PBW 343 (3)> Pusa Gold (2)> HP 1744 (1), and the variety HD 2967 obtained zero score at harvest. Thus, it could be concluded that Wheat 231 emerged as the most physiologically efficient variety in the experiments. This variety possesses the adaptive traits, especially the higher NUE, yield and its attributes. Further, N-dose response studies revealed that N @ 150 kg ha-1 (as granule) or N @ 1500 ppm (as foliar spray) in the form of Urea was the most effective in augmenting the NUE and its related physiological indices including economic yield in wheat crop in Assam.
  • ThesisItemOpen Access
    ASSESSMENT OF CROP CONDITION UNDER ABIOTIC STRESS USING REMOTE SENSING TECHNIQUE IN UPLAND RICE CROP
    (AAU, Jorhat, 2017-09) Goswami, Jonali; Das, Ranjan
    The rise of CO2 (600 µmol mol-1 CO2 by the middle of 21st century) will effectively influence the productivity of crop plants. Again nitrogen is a limiting nutrient in most of the agricultural soils. In the present investigation, the responses of local genotypes of rice were studied in Carbondioxide Temperature Gradient Tunnel (CTGT) to simulate elevated CO2 concentration and temperature. Similarly, pot experiment was done at varying levels of nitrogen fertilization. The results revealed that CTGT II had a greater impact on various morpho-physiological parameters and showed a declining trend indicating the deleterious effect of high temperature, at a higher CO2 and temperature in CTGT III, but, some parameters viz. stomatal conductance and transpiration rate were significantly reduced at CTGT II. From the above experiment, it is evident that some degree of tolerance exhibited against high temperature stress which could be attributed to higher accumulation of carbohydrates, proline, adjustment of anatomical features, water status, better defense mechanism particularly in the genotype Inglongkiri followed by Banglami as compared to IET 22238 and Bash under elevated carbondioxide and temperature conditions. Similarly, nitrogenous fertilizer application had a statistically significant effect on yield components of rice genotypes. There was an increase in yield components with increase in each successive level of nitrogen fertilizers. Enhancement of yield components under high nitrogen levels might be attributed to vigorous and healthy growth, higher photosynthetic pigments, improved water relations and the formation of the membrane system of chloroplasts. Among rice genotypes Inglongkiri recorded significantly higher yield components at low nitrogen levels as compared to the Banglami, IET22238 and Bash. Further, it was revealed that hyperspectral remote sensing could effectively be used for detection of varietal performance, nutrient content and other biochemical parameters of the crop. It was observed that few specific bands were sensitive to chlorophyll (704,803 nm), protein (1514nm), nitrogen (2060, 2300nm), Fluorescence (730nm). Apart from specific bands, indices like NDVI, PSSRa, PSRI, FRI were highly correlated to different parameters of the crop. It is observed from regression analysis that yield can be estimated as function of different spectral indices with a R square value more the 0.7 under both the conditions at a statistical significance with 95% confidence level. A GUI has been developed for display and retrieval of information for the target plants which aided in generation of spectral data base to discriminate spectral behavior of commonly grown genotypes of rice in this region.
  • ThesisItemOpen Access
    Carbon sequestration through bamboo plantation
    (AAU, Jorhat, 2015) Choudhury, Hemendra; Kalita, P.
    A study was conducted during 2011-13 in i) inside open top chambers (OTC) with four CO2 concentrations (control, 380, 550 and 750 ppm), ii) in the experimental bamboo field of B N College of Agriculture, AAU Biswanth Chariali (OST) and iii) in farmer’s field of Biswanath Chariali (OFT) to assess the carbon sequestration potential of four bamboo species viz. S1: Bambusa tulda, S2: B. nutans, S3: B. balcooa and S4: Dendrocalamus hamiltonii. The result revealed that with the increase in CO2 concentration from 380 ppm to 750 ppm the seedling height, seedling girth, number of shoot/seedling, phyllochron, leaf number and leaf area/seedling, weight of leaf, branch, main stem, rhizome and whole seedling, specific leaf weight, relative leaf water content, stomatal frequency, stomatal index, stomatal pore area, chlorophyll content, chlorophyll stability index, carotenoid content, Rubisco activity, photosynthetic rate, content of starch, reducing and nonreducing sugar total sugar, non structural carbohydrate and soluble protein of bamboo seedlings showed better results in all four bamboo species tested. Being C3 plant the bamboo seedlings increase the rate of photosynthesis at elevated CO2 concentration and thereby register the higher values of growth parameters. At OST and OFT the S4 maintained the highest average culm height, DBH of culm, cum thickness, RLWC, stomatal frequency, stomatal index, percent pore area of leaf, LAI, Rubisco activity, rate of photosynthesis, content of chlorophyll, carotenoid, soluble protein, starch, total sugar in leaf and content of C, N, P, K in leaf branch, culm and in rhizome. In contrast the S1 registered the poorest performances among all four bamboo species. The S4 produced the highest (260.25 ton/ha) total dry biomass with the highest leaf (7.16 ton/ha), branch (24.83 ton/ha), culm (207.64 ton/ha) and rhizome (10.98 ton/ha) biomass while S1 produced the lowest (212.10 ton/ha) total dry biomass with lowest leaf (3.88 ton/ha), branch (20.36 ton/ha), culm (171.84 ton/ha) and rhizome (8.86 ton/ha) biomass. S4 sequestered the highest total carbon (118.72 ton/ha) followed by S3 (110.00 ton/ha) and S1 sequestered the lowest total carbon (93.52 ton/ha) at 5th year of plantation. The S4 also produced the highest average leaf (1.35 kg), sheath (0.90 kg) and branch (0.12 kg) litter per clump. The bamboo plantation also enriched the soil with organic carbon, available N, P, and K up to 50 cm soil depth. The present study therefore, concludes that bamboo being an efficient carbon sequesterer could contribute tremendously to mitigate the climate change, besides providing other economic and social contributions.
  • ThesisItemOpen Access
    Interactive effect of elevated carbondioxide and temperature on physiological characterisation of bhoot jolokia (Capsicum chinense Jacq.)
    (AAU, Jorhat, 2015) Das, Sangita; Das, Ranjan
    A study was conducted during 2012-2014 in Carbondioxide Temperature Gradient Tunnels (CTGTs) and in ambient condition to assess the interaction effect of elevated carbondioxide and temperature in two cultivars of Capsicum chinense Jacq. The treatments consisted of field (ambient CO2 and ambient temperature), CTGT I (380 ppm CO2 and ambient temperature), CTGT II (550 ppm CO2 with ambient temp. + 20 C elevation) and CTGT III (750 ppm CO2 with ambient temp. + 40C elevation). The elevation of temperature as compared to ambient was maintained from flower bud initiation to maturity stage. The results revealed that CTGT II had a greater impact on the various morpho physiological parameters viz. plant height, leaf number, branch number, plant spread, leaf area index, leaf area duration, specific leaf weight, root: shoot ratio, relative leaf water content, water potential, osmotic potential, C: N ratio, photosynthesis and chlorophyll stability index. However, at a higher CO2 and temperature in CTGT III, most of the above parameters showed a declining trend indicating the deleterious effect of high temperature. But, some parameters viz. senescence index, stomatal conductance and transpiration rate were significantly reduced at CTGT II. The phyllochron index was significantly reduced in both the elevated CO2 and temperature treatments. Similarly in biochemical parameters viz. accumulation of sugars, starch, total non structural carbohydrate, proline, antioxidant enzymes, capsaicin content showed a significantly higher value in both CTGTs as compared to ambient condition. A decline in pigments content of leaves such as chlorophyll and carotenoid were observed under both the CTGTs. A better status of membrane was recorded at CTGT II as compared to CTGT III and ambient condition in terms of lower activity of lipoxygenase, MDA (Lipid peroxidation) and H2O2 content and relative stress injury; hence a higher value of membrane stability index was recorded in CTGT-II. An enhancement in flowering was observed in both the elevated CO2 and temperature than ambient condition. Anatomical studies revealed better pollen characters; stem xylem and phloem status in CTGT II when compared to CTGT III. RT PCR data showed up-regulation of some genes viz. photosynthetic genes, antioxidant genes and capsaicin synthase gene under both the CTGTs. Better yield attributing parameters were recorded in CTGT II as compared to CTGT III and other treatments, which could be correlated to a higher percent of fruit dropping in CTGT III. Reduction due to high temperature in terms of above parameters was ameliorated under CTGT II. From the above experiment, it is evident that some degree of tolerance exhibited against high temperature stress which could be attributed to higher accumulation of carbohydrate, proline, adjustment of anatomical features, modification of antioxidant pool, better water status, lower fruit drop percent, in cv. Manipur as compared to cv. Assam under elevated carbondioxide and temperature condition. This indicates the differential responses of genotypes under future climate change conditions.