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ThesisItem Open Access Effect of entomopathogenic fungi, Metarhizium anisopliae on growth and yield of french bean(AAU, Jorhat, 2017-07) Kurmi, Himanshu; Das, K.Experiments were carried out during September, 2016 to March, 2017 in the Department of Crop Physiology, Assam Agricultural University, Jorhat-13, to evaluate the effects of different concentrations of fungal isolate of Metarhizium anisopliae on french bean. In one experiment, fungal isolates of M. anisopliae at 105cfu/ml, 106cfu/ml, 107cfu/ml and 108cfu/ml concentrations were tested to determine their effects on germination and subsequent seedling growth of french bean under laboratory condition. It was observed that germination percentage, germination index, vigour index, shoot and root length, fresh and dry weight of shoot and root were affected by M. anisopliae isolates in a concentration dependent manner. Among the applied concentrations, 106cfu/ml and 107cfu/ml concentrations were found to be more stimulatory compared to control. Another experiment was conducted in field condition with these two concentrations (106cfu/ml and 107cfu/ml concentrations) to evaluate their effects on growth and yield of french bean. Three different modes of application, viz. seed treatment (before sowing), foliar application (15 DAS, 30 DAS and 45 DAS) and combination of seed treatment and foliar application were employed. Recorded data revealed that plant growth of french bean in terms of plant height, leaf number, leaf area, root volume, shoot and root dry weights were increased by M. anisopliae. Physiological parameters such as relative leaf water content, total chlorophyll content and leaf N P K content of french bean were also positively influenced by the fungal isolate. However, no significant alteration was recorded in leaf proline content irrespective to mode of application and concentration of fungal isolate. Among the different treatments, 107cfu/ml concentration was found to be more stimulatory when applied as combination of seed treatment and foliar spray. At this treatment combination, pronounced stimulatory effects were recorded on yield and different yield attributing parameters of french bean. From the results of this investigation, it can be concluded that in addition to its well established protective role against insect pest, M. anisopliae also exhibits stimulatory effects on growth, development and yield of the crop.