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Subject: Animal Reproduction and Gynecology × Author: HAQUE, AINUL × End date: 2017 × Start date: 2017 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF CAPACITATED AND LIQUID BOAR SEMEN
    (Assam Agricultural University, Khanapara,Guwahati, 2017-07) HAQUE, AINUL; AHMED, K.
    A total of 24 ejaculates, collected twice in a week by simple fist method, six from each of the four trained and healthy crossbred Hampshire boars of one to two years of age maintained at ICAR-All India Coordinated Research Project (AICRP) on pig, College of Veterinary Science, AAU, Khanapara, Guwahati-22, were selected for the present study. The ejaculates were extended with Modena extender (1:3), hold at 22°C for 4 hours and preserved up to 120 hours at 15°C.The semen samples were evaluated at 0 (i.e. immediately after extension), 24, 48, 72, 96 and 120 hours of preservation. In the present study, the overall mean sperm motility showed a decline from 85.42% to 51.04%, live intact acrosome decreased from 95.32% to 76.09%, HOST reacted spermatozoa declined from 73.53% to 46.41%, sperm membrane protein decreased from 13.68 to 6.06 mg/109 spermatozoa, extracellular protein level in extender increased from 1.54 mg/ml to 1.84 mg/ml and sperm cholesterol level declined from 31.84 to 16.22 mg/108 spermatozoa. The overall mean values were found to be differed significantly (P ˂0.001) with increase in hours of preservation in the extender but no significant difference in overall mean extracellular protein level was observed between 24 hours and 48 hours as well as 72 hours and 96 hours of preservation. Sperms were suspended in TALP medium and incubated for 5 hours at 37°C for in vitro capacitation and evaluation was carried out at 0, 1, 2, 3, 4 and 5 hours of incubation. In the present study, the highest hyperactivation motility was observed at 4 hours of incubation from 18.96% at 0 hour to 71.86% at 4 hour, the hyperactivated motility of spermatozoa increased significantly up to 4 hours then it decreased significantly to 54.79% at 5 hours of incubation. The overall mean live intact acrosome declined from 94.76% to 39.06%, HOST reacted spermatozoa decreased from 76.32% to 60.59%, sperm membrane protein decreased from 24.90 to 11.40 mg/109 spermatozoa, extracellular protein in TALP medium increased from 0.49 to 1.07 mg/ml and sperm cholesterol level declined from 31.84 to 8.57 mg/108 spermatozoa. The overall mean values were found to be differed significantly (P ˂0.001) with increase in hours of incubation in the medium but no significant difference in overall mean extracellular protein level was observed from 1 hour to 3 hour of incubation. The aim of the present study was to determine the nature of capacitation-like changes during preservation by studying the morphological and biochemical characteristics, plasma membrane integrity of in-vitro capacitated and preserved boar spermatozoa. In the present study, the changes of the boar spermatozoa at 96 hours of preservation in respect of acrosomal status, plasma membrane integrity, membrane protein and cholesterol levels resembles with the changes of spermatozoa of in vitro capacitated for 3 hours of incubation at 37°C and maximum in vitro capacitation was observed at 4 hours of incubation at 37°C.