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20211
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Subject: Animal Reproduction, Gynaecology and Obstetrics × Author: Das, Prasanta Kumar × Start date: 2010 ×
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    ThesisItemOpen Access
    Effect of cryopreservation on semen biochemical parameters including lipid profile in beetal and Assam hill goat
    (2021-01) Das, Prasanta Kumar; Sinha, Sudip
    A total of 72 ejaculates comprising six ejaculates from each of 12 bucks (six Beetal and six Assam Hill Goat) were used to study the effect of cryopreservation on the physical and biochemical characteristics of semen including sperm ultrastructure. The physical characteristics of fresh semen viz., ejaculate volume, mass activity, initial sperm motility, live sperm, sperm concentration, cold shock resistance index, acrosomal integrity, HOSTreacted sperm and sperm abnormalities were studied by conventional methods. The biochemical characteristics viz., sodium, potassium, calcium, total cholesterol, total lipid, AST, ALT and lipid profile were studied in seminal plasma, and total cholesterol, total lipid and lipid profile were also studied in spermatozoa of fresh and frozen semen. Semen was extended in Optixcell extender and frozen in 0.25 ml straws using liquid nitrogen vapour and stored in liquid nitrogen. Each sample was evaluated on the following day for sperm motility, live sperm, acrosomal integrity and HOST-reacted sperm. The biochemical characteristics studied after freezing in extracellular medium were the same as in case of seminal plasma. Sperm ultrastructure was studied both in fresh and frozenthawed spermatozoa. Physical and biochemical characteristics of semen of Beetal and Assam Hill Goats were within normal range and differed significantly between breed for ejaculate volume (P<0.001), sperm concentration (P<0.001), cold shock resistance (P<0.05), Host-reacted sperm (P<0.01), sodium (P<0.001), potassium (P<0.001) and total lipid (P<0.001). Postthaw sperm parameters viz. sperm motility, live sperm, acrosomal integrity and HOST– reacted sperm, and content of biochemical constituents viz. sodium, potassium, calcium, total cholesterol and total lipid decreased after freezing in semen of both the breeds, while AST and ALT increased. Thirteen and fourteen fatty acids were identified in the seminal plasma of Beetal and Assam Hill Goat bucks, respectively. Pentanedioic acid was identified only in seminal plasma of Assam Hill Goat. In the extracellular medium of frozen Beetal semen, all the fatty acids of seminal plasma of fresh semen were present, however, in Assam Hill Goat bucks both Dodecadienoic acid and Pentanedioic acid which were present in seminal plasma of fresh semen were found to be absent in frozen semen. Breed variation was observed in respect of Pentanedioic acid of fresh semen. Freezing had significant effect in alteration of membrane stabilizing fatty acids viz. Erucic acid, carboceric acid, linoleic acid, tricontanoic acid, arachidic acid, eicosadienoic acid, margaric acid, mentanic acid, tetradecadienoate, tridecanoic acid, heneicosylic acid, cerotic acid and tricosanoic acid. Major ultrastructural changes in spermatozoa after freezing were separating and ruptured plasma membrane, fusion of plasma membrane with outer acrosomal membrane, swelling of acrosome and loss of acrosomal content. Based on the physical and biochemical parameters studied it could be concluded that cryopreservation of goat semen has deleterious effects on fatty acid profile of seminal plasma and sperm plasma membrane, and also on sperm ultrastructure in both Beetal and Assam Hill Goat. The proportion of loss of plasma membrane fatty acids after freezing was lower in Assam Hill Goat as compared to that of Beetal goat. Post-thaw semen quality in Assam Hill Goat was superior to that of Beetal goat.