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Subject: Animal Nutrition × Author: BORA, SUBHALAKSHMI × End date: 2019 × Start date: 2016 × Type: Thesis × Thesis Type: Ph.D ×
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    ThesisItemOpen Access
    EFFECT OF VITAMIN E AND SELENIUM FEED SUPPLEMENTS ON PERFORMANCE, OXIDATIVE STRESS, IMMUNITY AND HEAT SHOCK PROTEIN EXPRESSION IN BROILER CHICKEN
    (College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, 2019-07) BORA, SUBHALAKSHMI; Ahmed, H. F.
    The present study was undertaken to investigate the effect of dietary supplementation of vitamin E and Selenium on the growth performance, oxidative stress, immunity and heat shock protein expression in Broiler chicken. Two hundred and forty (240) day-old commercial Vencobb 400 broiler chicks were randomly divided into four experimental groups viz. T0 (unsupplemented control), T1 (Vitamin E @ 100 mg/kg and Selenium @ 0.2 mg/kg), T2 (Vitamin E @ 125 mg/kg and Selenium @ 0.25 mg/kg) and T3 (Vitamin E @ 150 mg/kg and Selenium @ 0.3 mg/kg) groups of 60 chicks each subdivided into 3 equal replicates. The experimental birds were offered ad libitum quantities of four experimental rations either without supplementation or with supplementation of different levels of vitamin E and selenium from 0 to 7 days, 8 to 21 days, and 22 to 42 days of age. During the experiment, weekly body weight and daily feed intake, total body weight gain, total feed intake, feed conversion ratio (FCR), survivability rate, BPEI, various blood haematological and bio-chemicals constituents, antioxidant enzymes, heat shock protein, immunity, cortisol, carcass characteristics like dressing percentage, cut up parts percentage, relative organ and giblets weight, physicochemical properties of breast meat were studied and recorded. The mean total body weight (g) was 2120.0±33.5, 2292.9±14.7, 2219.0±16.4 and 2179.9±9.08 and mean total weight gain (g) was 2077.7±14.5, 2250.6±14.7, 2176.6±14.3 and 2137.6±9.08 for T0, T1, T2 and T3 groups, respectively. The mean total feed intake (g) was 4062.75±0.08, 4156.53±0.77, 4151.91±0.35 and 4117.85±11.67 and the average feed conversion ratios was 1.92±0.04, 1.81±0.01, 1.88±0.04 and 1.87±0.04 for T0, T1, T2 and T3 groups, respectively. T1 group attained significantly higher (P0.05) with T3 group; but, had a significantly higher (P0.05) in mean total Feed Conversion ratio. BPEI was found to be significantly (P0.05) difference was observed among T0 (110.67±4.67), T2 (118.00±0.58) and T3 (116.33±0.33) groups. Economics of production showed that difference in the total feed cost (Rs.) and the total cost involved per bird (Rs.) were highly significant (P0.05) among the experimental groups. Overall mean haematological values was, for Hb (%) 9.75± 0.50, 11.22± 1.56, 10.56±1.11 and 10.50±1.19; for PCV (%) 25.7±0.60, 28.22±1.67, 27.20±1.01 and 27.23±0.86 and for WBC (thousand mm3) 28.03±2.95, 24.13±0.47, 25.13± 0.75 and 25.14±0.83 for T0, T1, T2 and T3 groups respectively. Hb (%), PCV (%), WBC (thousand mm3) level in T1 treatment group was significantly higher (P0.05) but, were significantly higher (P0.05) but, were significantly higher (P0.05) AST (U/L) and ALT (U/L) level than rest of the groups. The overall mean values of serum superoxide dismutase (SOD, unit/mg protein) was 2.03±0.39, 3.46±0.64, 2.94±0.68 and 2.69±0.45; serum GPx (microgram/mg protein) was 2.37±0.27, 3.61±0.18, 2.99±0.27 and 2.83±0.30 and serum GSH (mg/g protein) was 0.90±0.07, 1.36±0.53, 1.21±0.41and 1.17±0.36, respectively in T0, T1, T2 and T3 groups, respectively. SOD, GPx and GSH levels in T1 group were significantly higher (P0.05); but, were significantly lower (P0.05). The carcass parameters like dressing percentage with giblet in T0, T1, T2, T3 groups was 77.42±0.21, 80.13±0.59, 78.64±0.09 and 78.54±0.21, respectively. The dressing percentage without giblet in T0, T1, T2, and T3 groups was 71.86±0.25, 74.79±0.53, 73.12±0.05 and 72.87±0.24, respectively. Dressing percentages were significantly higher (P0.05). The abdominal fat % in T1 (2.09±0.50) group was significantly lower (P0.05). In breast meat the pH was 5.47±0.19, 6.14±0.09, 5.79±0.06 and 5.74±0.19; WHC(%) was 58.17±1.83, 72.38±1.25, 65.08±1.70 and 67.95±1.45; drip loss (%) was 6.96±0.48, 4.71±0.45, 6.08±0.46 and 5.70±0.39; SFV (Kg/cm2) was 3.80±0.10, 2.77±0.38, 3.23±0.15 and 3.30±0.10 and TBARS (mg MDA/Kg) was 3.30±0.10, 1.18±0.04, 3.23±0.15 and 2.77±0.38 in T0, T1, T2 and T3 groups, respectively. PH and WHC (%) level in T1 group was significantly higher (P0.05) but, were significantly higher (P0.05).