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20171
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Subject: Animal Biotechnology × Author: BASUMATARY, KELESON × Start date: 2010 ×
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    ThesisItemOpen Access
    POLYMORPHISM OF PROLACTIN (PRL) AND PROLACTIN RECEPTOR (PRLR) GENES IN INDIGENOUS DUCKS OF ASSAM
    (Assam Agricultural University, Khanapara,Guwahati, 2017-07) BASUMATARY, KELESON; Das, Bula
    The present study was conducted to investigate the polymorphism of Prolactin (PRL) and Prolactin receptor (PRLR) genes in 101 indigenous ducks of Assam. PCR-RFLP analysis of PRL gene using restriction enzyme DraI revealed three genotypes, arbitrarily designated as AA, AB and BB. Following digestion, the AA genotype yielded two fragments (141 and 316 bp), the AB genotype yielded three fragments (141, 316 and 457 bp), and the BB genotype yielded one fragment (457 bp). The frequencies of A and B alleles were found to be 0.847 and 0.154 respectively and the genotype frequencies for AA, AB and BB genotypes were found to be 0.812, 0.069 and 0.119, respectively. The PCR-RFLP analysis of PRLR gene using restriction enzyme PciI revealed two genotypes, arbitrarily designated as AA and AB. The AA genotype yielded two fragments (108 and 259 bp) and the AB genotype yielded three fragments (108, 259 and 367 bp). The frequencies of A and B alleles were found to be 0.956 and 0.045, respectively and the genotype frequencies for AA and AB genotypes were found to be 0.911 and 0.089, respectively. On the basis of the present study, it was found that the A variant of PRL gene was predominant in the indigenous ducks of Assam with the highest frequency of AA genotype followed by BB genotype (AA>BB>AB). For PRLR gene, the frequency of allele A was higher than that of allele B with a higher frequency of AA genotype (AA>AB). Chi-square (χ2) test revealed that the population under study was not in Hardy-Weinberg Equilibrium for PRL gene, while for PRLR gene, the population was in Hardy-Weinberg equilibrium.