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ThesisItem Open Access EFFECT OF SUPPLEMENTATION OF Aegle marmelos AND Murraya koenigii LEAVES OON OVARIAN FUNCTION AND FERTILITY RESPONSE IN ANESTROUS CATTLE(West Bengal University of Animal & Fishery Sciences, Kolkata, 2022) MANDAL, DURGADAS; Ray, Kalyani; Misra, P.C.; Basu, Siddhartha; Das, Goutam Kumar; Batabyal, Subhasis; Pal, ArunaThe proposed work was carried out at Livestock Farm Complex, Mohanpur, WBUAFS to assess the effect of supplementation of Aegle marmelos (Bel) and Murraya koenigii (curry) leaves supplementation on ovarian function and fertility response in anestrous cattle. In farm trial- Total 16 animals (Sahiwal anestrous cow) were selected and divided randomly into two equal groups (n = 8). The shed dried leaves (200 gm) of A marmelos and M koenigii were fed to cows of group I treatment group for 5 days and Group Il considered as untreated control. In field trial 30 postpartum anoestrous cows were randomly divided into two equal groups, comprising fifteen (15) animals in each group. In treatment group, experiment animals were fed with herbal preparation as described above under farm trial. Blood samples were collected on Day-0, 4,9, on the day of estrus, day 8 after onset of estrus and 21 days after onset of estrus from all the experimental animals of farm trials for determination of serum level of Antioxidants MDA, ASCA, TAC, trace element Zn, Mn, Cu, Se, I, Fe. And progesterone and estradiol hormones. Al was done to the cows exhibited estrus following treatment and perform per - rectal examination for confirmation of pregnancy. All data were analysed statistically. Under farm trial 100% cows from treated group and 37.5% in control group exhibited estrus. The men interval between the first day of treatment and onset of estrus were 19.25 and 61 days in treated and control group respectively. The main duration of estrus were 13.75 and 14.67 hrs in treated and control group respectively. Under field trial 86.7% cows from treated group and 20% in control group exhibited estrus. The men interval between the first day of treatment and onset of estrus were 21.77 and 72 days in treated and control group respectively. The main duration of estrus were 14 and 13.33 hrs in treated and control group respectively. The overall conception rate (CR) was 75% and 12.5 % in treated and control group respectively under farm trial. Whereas in field trial the overall CR was 46.46% and 20% in treated and control group respectively. Ascorbic acid and total anti-oxidants capacity increased whereas Malondialdehyde level was decreased after supplementation of herbs. Copper, iodine, Iron, Zinc and selenium level increased whereas no change in manganese level was found after supplementation with Aegle marmelos and Murraya koenigii than the untreated control cows. The level of progesterone and estrogen increased at certain level. Results of present study suggest that Aegle marmelos and Murraya koenigii combined treatment restored appreciable fertility in terms of occurrence of estrus and pregnancy establishment in anestrous cowsThesisItem Open Access Comparative Clinical Evaluation and Uterine Hemodynamics of Different Treatment Protocols in Canine Pyometra(2022) Sarkar, Pradip; Basu, Siddhartha; Patra, Bijay Kumar; Ray, Kalyani; Batabyal, Subhasis; Ghosh, Prabal Ranjan; Mukherjee, JoydipThe focus of the present research is to highlight findings on effectiveness of three different medical protocols to treat pyometra, comparative study of the uterine hemodynamic and vascularisation analysis through Doppler ultrasound within the groups and assess the serum concentration of CRP in canine pyometra in order to evaluate the possibility of application as a diagnostic and prognostic tool in this syndrome. The present research was undertaken on 64 bitches of different breeds with known breeding history with either open or close type of pyometra. The bitches were randomly divided into four groups consisting three treatment group and one control group consisting of sixteen animals each. The animals of group 1 (n=16) were treated using combination of a progesterone receptor antagonist i.e. Mifepristone @ 3 mg/kg PO BD for consecutive 3 days starting from 0 day and the dopamine agonist prolactin inhibiting drug, i.e., Cabergoline @ 5μg/kg PO OD for 10 consecutive days starting from 0 day along with supportive therapies i.e., Antibiotic like Amoxycillin plus Clavulanic Acid @ 25mg/kg/day for 7 days, Ringer’s lactate for hydration and others used as per symptoms showed. While the bitches in the group II (n=16) received the same as of the first group along with Misoprostol @ 10 μg/kg PO BD for 3 consecutive days starting from day 1. Whereas, the animals of group III (n=16), were treated with progesterone receptor antagonist i.e. Mifepristone @ 3 mg/kg PO BD for 3 consecutive days starting from 0 day and a lower dose of synthetic PGF2α (Cloprostenol) will be given @ 1μg/kg SC OD from 2nd day to 6th day with all supportive therapies as above. The animals in group IV (n=16) were served as control and received Antibiotic and same supportive therapies as were given to other three experimental groups. Blood samples were collected from all the animals at pre and post-treatment for the estimation of haematological, biochemical and immunological parameters. TLC and Neutrophil % declined significantly (P<0.05) in all the treatment group. A significant (P<0.05) decrease in ESR value was also observed in all the treatment groups. C Reactive protein (CRP) decline in all the treatment group and it showed highly significant (P<0.01). Similarly, Uterine lumen diameter, peak systolic value (PSV) and end diastolic value (EDV) of uterine arteries were decreased significantly (P<0.01) at post treatment in all treatment group except control group. Alternatively, after treatment, resistance index (RI) increased in all treatment group significantly (P<0.01) except control animals. The overall recovery rate was highest (87.5%) in animals treated with mifepristone, cabergoline and misoprostol followed by 68.75 percent each in group treated with mifepristone with cabergoline and mifepristone with cloprostenol, whereas, only 25 percent animals recovered from control group. This study indicates these medicinal protocols to treat canine pyometra could be a very good alternative over traditional ovariohysterectomy.ThesisItem Open Access Characterization of Mesenchymal Stem Cells From Canine Ovary and Endometrium and Screening of Suitable Cryopreservation Medium for Storage(West Bengal University of Animal & Fishery Sciences, Kolkata, 2021) Jha, Ajeet Kumar; Ray, KalyaniThesisItem Open Access Comparison of therapeutic efficacy of certaindrugs with special reference to the use of immunomodulators for ameliorating bovine endometritis(Kolkata, 2018) Dash, Somnath; Basu, Prof. (Dr.) Siddhartha; Sahatpire, Prof. (Dr.) Sunil K.; Mishra, Dr. Purna Chandra; Ray, Prof. (Dr.) Kalyani; Batabyal, Prof. (Dr.) Subhasis; Ghosh, Dr. Prabal RanjanThesisItem Open Access Effect of some micro-environmental factors of epididymis in relation to maturation of spermatozoa in Pigs (Sus scrofa)(2016) SOMRAJ, CHATTARAJ; Basu, Prof. (Dr.) Siddhartha; Mohanty, Prof. (Dr.) D. N.; Roy, Dr. (Mrs.) K.; Dutta, Dr. U.; Batabyal, Dr. S.; Ghosh, Dr. P. R.ThesisItem Open Access Cryopreservation of in-situ jamunapari buck cauda epididymal spermatozoa with electrolyte free medium : a novel perspective to save the biodiversity(Kolkata, 2014) Podder, Suman; Datta, Dr. U.; Mohanty, Prof. D.N.; Basu, Prof. S.; Nandi, Dr. P. R.; Ray, Dr. (Mrs.) K.; Ray, Prof. S.K.; Batabayal, Dr. S.ThesisItem Open Access STUDIES ON CRYOPERSERVATION OF SEMEN: EFFECT OF DIFFERENT ADDITIVES ON POST THOWING SEMINAL ATTRIBUTES OF BLACK BENGAL BUCK(CAPRA HIRCUSBENGALENSIS) AND GAROLE RAM(Ovis aries)(Kolkata, 2004) Mukherjee, Krishna Prasad; Basu, Dr. Siddhartha; Bandyopadhyay, Dr. S. K.; Sahoo, Dr. A. K.; Ray, Dr. S. K.; Nandi, Dr. P. R.; Dutta, Dr. U.ThesisItem Open Access Topographical distribution of calcium, cholesterol, estrogen and vitamin D3in the Jamunapari buck (Capra hircus) excurrent duct and their relationship with spermatozoan motility(Kolkata, 2013) Ranjan, Ashish; Dutta, Dr. U.; Bandyopadhyay, Prof. S. K.; Basu, Prof. S.; Ray, Prof. S.; Nandi, Dr. P. R; Ray, Dr. (Mrs.) K.; Batabayal, Dr. S.ThesisItem Open Access DnlCTION OF ISll PRODICII. l. e,u DO DTIMICROIIil HISISTDeE II UTERIIE INFECTI.N.F CAnlE(Kolkata, 2013) SARKAR, BIDHAN; Roy, Dr. Kalyani; Ghosh, Prof. B. B.; Naskar, Dr. S.; Basu, Prof. S.; Dutta, Dr. U.; Nandi, Dr. P. R.
