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Subject: Animal Physiology ×

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Now showing 1 - 9 of 18
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    ThesisItemOpen Access
    EFFECT OF MICRO-NUTRIENTS ON MORPHO-PHYSIOLOGICAL PARAMETERS AND YIELD IN BLACK GRAM (Vigna mungo L.)
    (DEPARTMENT OF PLANT PHYSIOLOGY B. A. COLLEGE OF AGRICULTURE ANAND AGRICULTURAL UNIVERSITY ANAND, 2018) Mahammad Aman Z. Malek; Dr. D. B. Patel
    The present investigation entitled “Effect of micro-nutrients on morpho-physiological parameters and yield in black gram (Vigna mungo L.)” was carried out during 2017 at Department of Plant Physiology, B. A. College of Agriculture, Anand Agricultural University, Anand. The experiment was laid out in completely randomized block design with four replications to known the effect of micronutrients on various morpho-physiological, biochemical, yield and yield components. The treatments consist of control (T1) multi-micronutrient mixture (MN) grade-1 general (foliar spray) (T2), MN mixture grade-2 for Zn deficiency (foliar spray) (T3), MN mixture grade-3 for Fe deficiency (foliar spray) (T4), MN mixture grade-4 for Fe and Zn deficiency (foliar spray) (T5), MN mixture grade-5 general (soil application) (T6) and micronutrient application as per soil test value (STV) (T7).
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    ThesisItemOpen Access
    RELATIONSHIP BETWEEN SOME CLIMATIC ELEMENTS AND PHYSIOLOGICAL RESPONSES IN KANKREJ CALVES
    (AAU, Anand, 1970) Tambat, Bapu Bhikarambhai; Patel, U. G.
    Abstract not Available
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    ThesisItemOpen Access
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    ThesisItemOpen Access
    PHYSIOLOGICAL REACTIONS AND BLOOD CHARACTERS IN SURTI BUFFALO CALVES AS RELATED TO SOME CLIMATIC FACTORS
    (AAU, Anand, 1967) Dubey, Vishnu Kumar; Patel, J. R.
    Abstract not Available
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    ThesisItemOpen Access
    VARIATIONS IN THE GONADOTROPHIC HORMONE LEVEL IN THE ANTERIOR PITUITARY GLAND DURING THE OESTROUS CYCLE IN BUFFALO
    (AAU, Anand, 1970) Agarwal, Shanti Prasad; Buch, N. C.
    Abstract not Available
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    ThesisItemOpen Access
    HAEMATOLOGICAL, BIOCHEMICAL AND ENDOCRINE PARAMETERS AT DIFFERENT AGES AND PHYSIOLOGICAL STAGES IN GIR CATTLE AND JAFFARABADI BUFFALOES
    (AAU, Anand, 2012) JACOB, NINAN; ARYA, J. S.
    The study titled "Haematological, Biochemical and Endocrine parameters at different Ages and Physiological stages in Gir cattle and Jaffarabadi buffaloes" was imdertaken in different age groups and physiological stages in Gir cattle and Jaffarabadi buffaloes with the objective to determine and compare the species differences for (i) haematological parameters viz. TEC, Hb, PCV, TLC, DLC (ii) biochemical parameters viz. plasma glucose, total protein, albumin, total cholesterol, HDL cholesterol, triglycerides, urea, uric acid, creatinine, calcium, phosphorus, magnesium, sodium and potassium (iii) enzymes viz. aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, and lactate dehydrogenase (iv) hormonal levels of triiodothyronine, thyroxine, insulin, growth hormone, Cortisol, estradiol - 17 β, progesterone and testosterone and (v) to observe the milk components viz. percentage of fat, protein and lactose in lactating Gir cows and Jaffarabadi buffaloes and their relationship with the stage of lactation under study. The blood samples were collected from Gir and Jaffarabadi females (n=8 for each sampling stage) at 1 wk, 1, 3, 6, 12, 24 and 36 months age, at 1, 2 and 3 month of lactation and in non-lactating pregnant and non-pregnant animals. In Gir and Jaffarabadi males (n=6 for each sampling' stage) blood samples were collected at 1 wk, 1, 3, 6 and 12 month of age and in bulls. Castrated males were also used for blood sampling in Gir males. A total of 270 blood samples were collected and the analysis was conducted by standard techniques.
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    ThesisItemOpen Access
    DEVELOPMENT OF DIGESTIVE ENZYMES IN PANCREATIC JUICE AND DUODENAL FLUID OF COW CALVES AND BUFFALO CALVES FROM BERTH TO EARLY RUMINANT STAGE
    (AAU, Anand, 1996) Christi, K. S.; VADODARIA, V. P.
    Healthy 22 calves (10 cow calves; 12 buffalo calves) maintained at Veterinary college, Anand, with an objective of establishing physico-biochemical profile of pancreatic juice and duodenal fluid from birth to fifteen week of age were studied in relation to different physiological factors like age, feeding and diurnal variations. There is no report of research work carried out simultaneously both in cow calves and buffalo calves as well as on panoreatic juice and duodenal fluid at same age, feeding schedule and for diurnal variations in various parameters. The research data were classified and tested with the help of standard statistical procedures. Work on buffalo calves has not been found reported fluid, therefore the present work provides the basic informations for future research endeavor.
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    ThesisItemOpen Access
    BLOOD BIOCHEMICAL PROFILES DURING DIFFERENT STAGES OF GESTATION AND POSTPARTUM IN SHEEP AND GOAT
    (AAU, Anand, 2009) PANDYA, UNNATI D.; ARYA, J. S.
    A study was conducted on nine Patanwadi sheep and fourteen Surti goats at Insrtuctional Farm, Department of Livestock Production and Management, Veterinary College, Anand Agricultural University, Anand with the objectives to know various blood biochemical profiles at different stages of gestation and post partum. The blood was collected from these animals through jugular venipuncture once a day on days 30, 60, 90 and 120 post mating and on the day of parturition (day 0) and days 3, 7 and 15 post partum. The blood samples so collected were analysed for Haemoglobin, Packed Cell Volume, Blood Glucose, Plasma Total Protiens, Cholesterol, Triglycerides, Calcium, Phosphorus, Trace Minerals (Cu, Fe, Zn, Mn & Co), electrolytes (Sodium & Potassium) and Hormones (T3, T4, Insulin, Progesterone, Estrogen and Cortisol) using standard laboratory procedures. Triglycerides were estimated by kit (Crest Biosystems, Goa). Trace elements were estimated by using Atomic Absorption Spectrophotometer (ECIL, AAS4141). Hormones were analysed by using kits of ImmunoTech, France. Haemoglobin and PCV both decreased significantly (P<0.05) as the pregnancy advanced and increased non-significantly during post partum in both sheep and goats. In non-pregnant sheep and goats the values differed nonsignificantly on various day of blood collection. The values also did not differ between the pregnant and non-pregnant sheep and goats. Biochemical parameter such as blood glucose significantly (P<0.05) increased during pregnancy and decreased during post partum in sheep. In non-pregnant sheep and goats the values did not differ significantly. A nonsignificant decrease in glucose was seen in goats during pregnancy and post partum. Plasma total proteins showed a significant (P<0.05) decrease in pregnant and non-pregnant sheep and an increase during post partum. The totals protein non-significantly decreased in pregnant and non-pregnant goats while significantly (P<0.05) increased during post partum. Plasma cholesterol significantly (P<0.05) increased upto parturition and then decreased during post partum in pregnant sheep and goats. A non-significant decrease in plasma cholesterol was shown by non-pregnant sheep and goats at various stages of sample collection. Plasma triglycerides significantly (P<0.05) decreased during various stages of gestafion in sheep. A non-significant decrease in plasma triglycerides was seen in sheep during post partum and in non-pregnant animals as well as in goats during pregnancy and post partum. In biochemical profiles a non-significant difference was shown for all parameters (except pregnant sheep for blood glucose) between pregnant and non-pregnant sheep and goats. Plasma calcium significantly (P<0.05) decreased in pregnant sheep while increased during post partum. A non-significant decrease was shown by non-pregnant sheep and goats and pregnant goats. The plasma calcium increased significantly (P<0.05) during post partum. Plasma inorganic phosphorus significantly (P<0.05) decreased in pregnant and non-pregnant sheep and did not differ during post partum. The plasma phosphorus did not differ in goats during pregnancy and post partum while significantly decreased in non-pregnant goats. A non-significant difference was shown between pregnant and non-pregnant sheep and goats in levels of plasma calcium, inorganic phosphorus and trace minerals. However, plasma copper had a significant (P<0.05) variation due to stage of gestafion in both sheep and goats and zinc and cobalt had a significant (P<0.05) difference in goats only. While during post partum only Fe and Mn had significant (P<0.05) variation in both sheep and goats. : The plasma electrolytes, sodium and potassium increased significantly (P<0.05) during various stages of gestation but sodium decreased and potassium increased significantly (P<0.05) during post partum in sheep and goats. The plasma sodium of non-pregnant sheep and goats differed significantly (P<0.05) whereas plasma potassium did not differ. The plasma T3 showed a non-significant difference in both sheep and goats. The plasma T4 differed significantly (P<0.05) in sheep during pregnancy but showed a non-significant difference in pregnant, non-pregnant and post partum sheep and goats. Plasma insulin showed a significant (P<0.05) increase in non-pregnant goats and post partum sheep and goats. Non-pregnant sheep and pregnant sheep and goats showed a non-significant difference. A significant (P<0.05) difference was seen between pregnant and non-pregnant sheep and goats for plasma insulin. A non-significant difference was seen in plasma progesterone in pregnant, non- pregnant and post partum sheep and goats. The difference between pregnant and non-pregnant sheep and goats was significant (P<0.05) for plasma progesterone. Plasma estradiol significantly (P<0.05) increased in sheep during pregnancy and post partum. In nonpregnant sheep and post partum goats the difference was non-siginificant. A significant (P<0.05) difference was seen between pregnant and non-pregnant sheep whereas in goats the levels did not differ for plasma estradiol as well as Cortisol. A non-significant increase in plasma Cortisol was observed in pregnant and non-pregnant sheep and goats. There was a significant (P<0.05) decrease in the plasma Cortisol levels during post partum in sheep and goats. The average birth weight in male lambs was 2.5 ± 0.14 kg and that of female 2.8 kg while in goats the mean birth weight in male kids was 2.3 ± 0.07 kg and females 1.88 ± 0.3 kg. The gestation period for male and female lambs was 147 ± 0.84 and 147 days, respectively while in male and female kids was 145 ± 1.30 and 141 ± 1.08 days, respectively.
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    ThesisItemOpen Access
    STUDY ON BLOOD BIOCHEMISTRY IN RELATION TO MILK PROTEIN WITH SPECIAL REFERENCE TO KAPPA CASEIN IN INDEGENOUS GOAT
    (AAU, Anand, 2010) PATEL, SANJAYKUMAR B.; Pande, A. M.
    Caprine casein genes exiiibit an unusual and extensive polymorphism that affects milk quality and composition. The Kappa casein gene includes 5 exons, 4 of them carrj'ing more than 90 per cent of the information to encode for the mature protein. Caseins comprise about 80 per cent of the total protein content of milk and present polymorphism with changes in the amino acid sequence. Within this abundance of proteins. Kappa casein is noteworthy, since it has been associated with differences in milk yield, composition and processing. The objective of this study was to observe the existence of polymorphism in the Kappa casein gene and to compare the levels of plasma and milk biochemical parameters in lactation stage of Zalawadi goats. For this purpose, blood samples from 50 lactating goats, collected with vacutainer by needle puncture of the jugular vein. Plasma separated from blood and cellular parts (WBCs) was used for genomic DNA extraction. Milk samples were collected in glass test tube (50 ml capacity) v^ith boric acid preservative and milk samples were preserved at -20 °C till laboratory analyses. The milk biochemical profiles were estimated by using the ECOMILK analyzer and milk urea nitrogen and total casein were estimated by Auto method and kjeidahl method, respectively. The blood biochemical profiles were estimated by using the BS 120 CHEMISTRY analyzer. Plasma sodium and potassium were measured by Flame photometer. DNA was extracted from cellular part of blood. Goat Kappa Casein (CSN3) gene (exon 4) was amplified from genomic DNA samples by PCR. The PCRs were carried for the amplification of Kappa casein {CSN3) gene with specific primers (F: - 5' TCC CAA TGT TOT ACT TTC TTA ACA TC 3') and (R: - 5' GCG TTG TCC TCT TTG ATG TCT CCT TAG 3') gene loci. Amplified products were visualized as a single compact band of expected size of 645bp under UV light by gel documentation system. PCR products and restriction enzymes were incubated overnight in water bath at 37 °C for digestion with Haelll and Alw441 RE and at 65 °C for digestion with BseNI RE. Digested products were visualized as per restriction patterns with compact band size under UV light by gel documentation system. There were monomorphic restriction patterns with HaeIII-RFLP digestion, whereas polymorphic restriction patterns shown with BseeNI-RFLP and Alw44I-RFLP. In this study, genotypes AA, AG, GG ware observed with BseNI and CC, CT, TT with Alw44I in these animals except HaeIII RFLP. The frequency of allele A and allele G was 0.87 and 0.13, respectively and the frequency of allele C and allele T was 0.88 and 0.12, respectively. There were non-significant and positive correlations between blood glucose with milk fat, plasma total proteins with milk protein and milk casein and plasma total cholesterol with milk fat. There was non-significant and negative correlation between plasma triglycerides with milk fat. There was highly significant (P<0.01) and positive correlation between blood urea nitrogen and milk urea nitrogen.