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ThesisItem Open Access EVALUATION OF M4 MUTANT LINES IN PIGEON PEA (Cajanus cajan (L.) Millsp.)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-12-27) MISAL SANJAY SHAHAJI; Palshetkar M. G.; Sawardekar S. V.; Mane A. V.; Shinde B. D.The present investigation, entitled "EVALUATION OF M4 MUTANT LINES IN PIGEON PEA (Cajanus cajan (L.) Millsp.)" was conducted at research and education farm, department of Agriculture Botany, College of Agriculture, Dapoli during Kharif 2022. As a result of the current investigation, it is clear that a wide range of variability exists for various traits, along with high heritability and high genetic advance as percentage of the mean for significant yield traits.ThesisItem Open Access GENETIC STUDIES IN RICE (Oryza sativa L.subsp indica) UNDER SUBMERGED CONDITIONS(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-12-27) RANE KUSHALKUMAR KISHOR; Vanave P. B.; Palshetkar M. G.; Mahadik S. G.; Bedse T. J.Field experiment was conducted during Kharif, 2022 at Botany farm, Regional Agricultural Research Station, Karjat, Dist Raigad. The present study was conducted with objectives to assess genetic variability in morpho physiological characters under submerge condition, to study association among different traits and to estimate direct and indirect effects of various grain yield components through path coefficient analysis under submerge condition in thirty-nine genotypes of rice (Oryza sativa L.). Based on the results of the experiment, it is observed that the genotypes showed significant genetic variability for all the characters under submerged condition, most of the characteristics showed high estimates of heritability and genetic advance, demonstrating the dominance of additive gene action. The genotypes Karjat 9, Ratnagiri purple, Ratnagiri 2, Sabita, Sahyadri and Swarna sub 1 were tolerant to submergence at 7-days and 14-days duration. In order to develop submergence tolerant variety, these genotypes may be effectively used in subsequent breeding programmes.ThesisItem Open Access ASSESMENT OF GENETIC VARIABILITY, CHARACTER ASSOCIATION AND DIVERSITY FOR YIELD AND ITS ATTRIBUTING TRAITS IN PIGEON PEA (Cajanus cajan L. Millsp.)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-12-22) BURHADE SANKET TULSIDAS; Palshetkar M. G.; Pethe U. B.; Mahadik S. G.; Rathod R. R.The present investigation, entitled “Assesment of genetic variability, character association and diversity for yield and its attributing traits in Pigeon pea’. (Cajanus cajan L. Millsp.)” was conducted at Research and Education farm, Department of Agriculture Botany, College of Agriculture, Dapoli during Kharif 2022. In general, phenotypic coefficient of variation (PCV) was higher in magnitude over genotypic coefficient of variation (GCV) for all the 14 characters. High phenotypic coefficient of variation was observed for the characters seed yield per plot, percent pod borer damage and plant height (cm). High genotypic coefficient of variation was observed in seed yield per plot, percent pod borer damage and number of pods per plant. Appreciable heritability estimates were observed for all the characters under evaluation. Highest heritability was observed for harvest index, test weight and days to 50 % flowering. ii Genetic advance found to be highest for seed yield per plot followed by percent pod borer damage. The character’s percent pod borer damage, seed yield per plot (g) and seed yield per plant (g) showed comparatively higher estimates of genetic advance as per cent of mean. The phenotypic correlation coefficient was higher in magnitude than their genotypic counter parts for most of characters. Number of pods per plant, Harvest index and days to maturity exhibited highly significant positive correlation with seed yield per plant at both phenotypic and genotypic levels. The character’s test weight and days to 50 % flowering had non-significant positive correlation with seed yield per plant while percent pod borer damage had significant negative correlation with seed yield per plant at both phenotypic and genotypic level. The path coefficient reported that the character’s test weight, days to 50 % flowering, number of branches per plant, number of seeds per pod, number of pods per plant, seed yield per plot, harvest index had positive direct effect on seed yield at both phenotypic and genotypic levels, while days to flower initiation, plant height and protein content had negative direct effect on seed yield per plant at genotypic and phenotypic levels. Forty-one genotypes were grouped into 6 different clusters on the basis of magnitude of D2 values evaluated by Mahanlanobis D2 analysis. Among forty-one genotypes, 27 genotypes were clustered into first (I) cluster, followed by cluster (II) with 6 genotypes, 5 genotypes were clustered in cluster (III), cluster (IV), cluster (V) and cluster (VI) had 1 genotype each. Inter cluster distance i.e., Divergence was highest between clusters III and VI (16.22), while intra cluster distance was maximum for cluster II (7.48). The genotypes LOCAL-7 followed by IPAWD-36 and IPAWD-25 were observed as best performers in the genotypes studied. These had the highest seed yield per plant and also maximum yield attributing characters. All the genotypes mentioned above have the potential to be used as promising genetic material in future breeding programmes.ThesisItem Open Access LINE X TESTER ANALYSIS IN COWPEA (Vigna unguiculata (L.) Walp)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-12-22) CHAUGULE OMKAR KRISHNA; Pethe U. B.; Palshetkar M. G.; More S. S.; Kale S. N.The present investigation entitled "LINE X TESTER ANALYSIS IN COWPEA (Vigna unguiculata (L.) Walp)" was undertaken with a view to study the parental diversity among parents through the study of heterosis, combining ability for yield and yield contributing characters in cowpea (Vigna unguiculata (L.) Walp).ThesisItem Open Access GENETIC VARIABILITY AND DIVERSITY FOR YIELD AND NUTRITIONAL TRAITS OF FINGER MILLET (Eleucine coracana (L.) Gaertn)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2024-01-10) GAIKWAD SANJYOT SHANKAR; Kunkerkar R. L.; Desai S. S.; Dhopavkar R. V.; Kadam J. J.The present experiment entitled "GENETIC VARIABILITY AND DIVERSITY FOR YIELD AND NUTRITIONAL TRAITS OF FINGER MILLET (Eleucine coracana (L.) Gaertn)" was undertaken to assess the genetic variability, genetic diversity correlation and path analysis in thirty-three of finger millet in compact family block design with two replications at Educational and Experimental research farm, Department of Agril. Botany, College of Agriculture, Dapoli, Dist Ratnagiri, Maharashtra during kharif 2022-2023.ThesisItem Open Access GENOMICS INTERVENTION TO IDENTIFY CANDIDATE GENES FOR SALT TOLERANCE IN RICE (Oryza sativa L.)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-08-14) MARASKOLE SHUBHANGI KSHIRSAGAR; Sawardekar S. V.; Singh A. K.; Waghmode B. D.; Mane A. V.; Kasture M. C.Twenty rice genotypes including Salt-tolerant (FL 478) and salt-sensitive (Karjat 4) checks were used for assessment of trait associated with salinity tolerance at 3 different salinity levels of 3 dSm-1, 6 dSm-1 and 9 dSm-1 through high-throughput digital phenomics facility available at ICAR-National Institute of Abiotic Stress Management, Baramati at the seedling stage. The result demonstrated that salt stress severely affects the morpho-physiological performance of the genotype, which is more sensitive to salt than salt-tolerant genotypes. This severe salt stress also affects biochemical parameters in selected twenty genotype with the same trend of genotypes. However, a significant decrease in potassium content and increasing sodium content in the shoots and roots in salt-sensitive genotpyes were observed as compared to salt-tolerant genotypes. It also poses other effects such as reduction in Chlorophyll content in leaves, affecting the Na/K ratio in both shoot and root. The screening based on morpho-physiological and biochemical analyses was used to simplify the complex salinity tolerance picture by categorizing the genotypes according to the level of tolerance exhibited where Kala rata, CST 7-1, SR 3-9, Damodar, CSR 36 and Karjat 184 genotypes performed better as compared to check FL 478 (Salt-tolerant) and Karjat 4 (Salt-sensitive). Several genes respond to severe salinity stress get up-regulated and down- regulated, the products of which are directly or indirectly involved in the protection of salt-tolerant and salt-sensitive germplasm. The present research looked into the differential gene expression response through transcriptome profiling of Saltol, OsNHX1, OsHKT1, OsHKT4, OsMYBS3, OsPDH45, OsCCC1, OsSKC1, OsMPK4, OsKCO1, OsTPC1, OsCDPK7, OsTIP1, OsCLC1 and OsAKT1 genes in selected desirable nine rice genotypes including six tolerant (Kala rata, CST 7-1, Damodar, FL 478, SR 3-9 and CSR 36) and three susceptible (Karjat 184, Karjat 4 and Ratnagiri 6) genotypes. More genes were found to be differentially regulated in tolerant than susceptible genotypes, with a substantial commonality between these genotypes for the traits under salt stress compared to normal conditions. Further 14 candidate genes were identified by RNA-Seq of CST 7-1, CSR 36, SR 3-9 and Karjat 184 genotypes and mapped to the reference genome under control and salt stress condition in comparison with FL 478 (Salt-tolerant) and Karjat 4 (Salt-sensitive) check.ThesisItem Open Access VARIABILITY AND CHARACTER ASSOCIATION STUDIES IN M3 GENERATION OF PIGEON PEA (Cajanus cajan L. MILLSP.)(Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-02-10) RUPANNAVAR, SALONI BABASAHEB; PALSHETKAR, M. G.; Pethe, U.B.; DESHPANDE, R.S.; Sawardekar, S.V.; Kunkerkar, R.L.ThesisItem Open Access GENETIC EVALUATION OF M3 POPULATION OF PIGEON PEA (Cajanus cajan (L.) MILLSPAUGH) THROUGH MOLECULAR MARKERS(Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2023-02-09) Bhosale, Mrunalini Anand; SAWARDEKAR, S. V.; Pethe, U.B.; Mahadik, S.G.; Palshetkar, M.G.ThesisItem Open Access ASSESSMENT OF GENETIC VARIABILITY FOR GRAIN YIELD AND ITS COMPONENTS IN FINE RICE (Oryza sativa L.)(Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 2022-11) CHENDAKE, SHUBHAM ANNASO; KUNKERKAR, R. L.; Desai, S. S.; Chavan, S. S.; Dhopavkar, R.V.Field experiment was conducted during Kharif, 2021 at Education and Research farm, Department of Agricultural Botany, College of Agriculture, Dapoli, Ratnagiri. The current study was conducted with objectives to estimate the genetic variability for yield and yield attributing characters and to estimate the genotypic and phenotypic correlation coefficient of different characters in thirty-two genotypes of rice (Oryza sativa L.). Based on the results of the current investigation, it is observed that the genotypes showed significant genetic variability for all the characters under study linked to the yield, most of the characteristics showed high estimates of heritability and genetic advance, demonstrating the dominance of additive gene action. The genotypes Palghar-1, BARCKKV-13, RP 6334-111-5-2-1, CR 4069-111-1-1-4-5-7, Ratnagiri-6 and SKL-07-11-177-50-65-60-14 were identified as the best for yield and yield attributing characters. In order to create a new high yielding, good quality fine rice variety these genotypes may be effectively used as parents in subsequent breeding programmes.
