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Master Degree Theses
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ThesisItem Open Access In Vitro Studies in Turmeric (Curcuma langa L.)(Agricultural Botany Department, N. M. College of Agriculture Gujarat Agricultural University, 2006-10) Shantilal, Patel Dharmendra Kumar; Shah, R. RThe present investigation entitled "In vitro studies in turmeric (Curcuma longa L.)" was carried out at the plant tissue culture laboratory, ASPEE College of Horticulture and Forestry, Navsari Agricultural University, Gujarat during the year 2004- 2005. The results reveled that emerging buds (5-10 mm long) from fully mature rhizomes of Curcuma longa L., stored in gunny bags (at ambient temperature) for 2-3 months to encourage bud development, were suitable as explants for micropropagation. The sterilization treatment involving initial shaking of explants with 5 per cent sodium hypochlorite for 5 minutes followed by dipping in 70 per cent ethanol for 30 seconds and finally treated with 0.1 per cent HgCl2 for 3 minutes resulted in minimum contamination and maximum establ ishment of the explants. Mercuric chloride at 0.1 per cent for 10 minutes reduced the contamination (5%) but increased death of culture (50%). Sodium hypochlorite alone failed to control the contamination. I , • • The treatment, MS medium supplemented with 2.0 mg!1 BAP and I mg!1 NAA was the best for culture establishment from the emerging bud explant while MS basal medium failed to respond . For the proliferation of the regenerated shoots, treatment MS medium containing 4.0 mg!1 BAP and 1.0 mg!1 NAA registered highest number of shoots (3.45) which constantly increased in subsequent subcultures. The same treatment produced maximum shoots (467.79) after four subcultures in serial subculturing of 4 weeks duration. When higher level (6 mg!l) of BAP was used, initially multiplication rate was higher but it was considerably reduced after 2nd subculture. The solidified nutrient medium with pH level 5.8 and 2 per cent "AR" grade sucrose was found to be optimum for maximum establishment, rate of multiplication, growth and vigour of the multiplied shoots. Half strength MS medium supplemented with 0.5 mg!1 IBA recorded cent per cent rooting and highest number of roots (6.00). IBA at 0 .5 and 1.0 mgfl produced long, thick and fibrous roots. On the other hand, 0.1 mgfi IBA produced thin and fibrous roots in both half and full strength MS medium. Group of plants (40-50) in poly trays covered with polythene tent and kept inside net house was found suitable for acclimatization of in vitro plants. The acclimatization of the rooted shoots was best in polythene bags containing Sand: Soil: FYM (I: I: 1 v/ v/v) as potting mixture. It resulted in highest