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ThesisItem Open Access Variability and D2-Analysis in Ragi (Eleusine coracana gaertn)(Plant Breeding and Genetics Department, N. M. College of Agriculture Gujarat Agriculture University Navsari, 1988-09) Patel, Jagdishchandra S.; Patel, R. HThesisItem Open Access Micropropagation of Papaya (Carica Papaya L.) Var. Honey Dew(Agricultural Botany Department, N. M. College of Agriculture Gujarat Agricultural University, 1997-07) Naik, Udayan I; Shah, R. RThe present investigation was carried out at the Plant Tissue Culture Laboratory attached to the Department of Botany, N.M. College of Agriculture, Gujarat Agricultural University, Navsari Campus, Navsari during 1995-97. It was aimed at to examine various aspects of micropropagation of papaya (Car iea papaya L.) va r. Honey Dew. The shoot tip explants WCrL found to be suitable for micropropagation of papaya. The treatment involving 0.1 % mercuric chloride solution for 5 minutes gave better sterilizatiorl of seedlj.rlg explar,t. Or) the other hand, for better results from mature explants 0.1 % mercuric chloride for 10 minutes found to be necessary. The trea t ment MS medium supplemented with 0.5 mg / l BAP and 0.01 mg/l NAA was ttle best for seedling shoot lip establishment arid proliferation. This treatment registered highest number of shoots (6.08 ~h o ots / explant) a mong all t he treatmerlts tried. In case of mature explants, it was the MS medium supplemented with 0.5 mg / l BAP and 0.1 m':J / l NAA which ga ve highest establishment (60 %) percentage. The same treatment also • gave higher r a te of prolifera tion ( 2 • 5 shoots/explant). Difficulties were encountered with mature explants for establishment due to latex prese nt in the explants. Washing the explants in rurlning water [or 3 hrs was found to be suitable to remove latex from the explants. Inspite of the se, establishment as well as proliferation of mat ure explants was poor as compared to the seedling explants. For serial subculturing of 3 weeks duration each changing the medium in each subc ulture alternately, basal and medium with 0.5 mg / l B~P plus 0.01 mg/l N~~ yielded • maXlmum shoots and longer shoots in all the subcultures. The combination of 1.S per cent fructose and 1.5 per cent glucose was the best carbohydrate sourc e for shoot proliferation. The initial pH 5.5 of the medium was more appropriate pH level as far as proliferation and shoot growth was concerned. The initial pH below 5.0 and above 6.0 adversely affected both prollferatiorl rate as well as growth of the shoots. Half strength MS medium ~upplemented with 0.1 mgll IS;:" and suc rose 3 per cent and sol i dified with 0.8 per cent agar was found to be the best for . -ln vitro rootin9· This treatment recorded 80 \ rootin9 though the number of days for root initiation was more than on the full strength MS medium supplemented with 0.1 mg/l NAA. The rooting was only 60 \ on NAA. t.re"ltment. Moreover, the plantlet.s raised on the medium with NAA. for rooting failed to c:l.cclimatize. Acclimatizing in culture room at 26 ! 20 C • USing glass beaker cover in soil, sand and FYM (1:1:1 v/v) potting mixture was found to be suitable for acclimatiziny pldntlets t;o the natural environment.ThesisItem Open Access Line x tester Analysis in Indian Bean (Lablab purpureus (L) Sweet)(Agricultural Botany Department, N. M. College of Agriculture Gujarat Agricultural University, 2002-10) Sukhbhai, Patel Vinaykumar; Naik, M. R.The present investigation on line x tester analysis was conducted in vegetable type Indian bean during rabi 2002 at College Farm, N.M. College of Agriculture, Gujarat Agricultural University, Navsari. Experimental material,'!omprising of four lines, eight testers and their thirty two hybrids were planted in randomized block design with three replications. Analysis of variance indicated highly significant differences for almost all the characters studied, revealing presence of considerable amount of genetic variability in the material tested. Similarly, hybrids also differed significantly for all the twelve characters. High heterotic effects were observed for pod yield per • plant, average pod weight, pods per plant, pods per raceme, pod length and seeds per pod. Crosses NV-96 x NV-40, NV-96 x NV--- . 11, Kapasi x NV-53, NV-96 x ND-2 and NV-96 x NV-53 were most heterotic hybrids for pod yield per plant. Increase in number of pods per plant mainly contributed towards the pod yield.. In some cross combinations average pod weight, pods per raceme, pod length and seeds per pod also contributed towards pod yield pCf plant. Combining ability analysis revealed the importance of both additive and non-additive variances for the expression of characters, however, additive type of gene action was predominant. The estimates of gca effects indicated that, Kapasi was a good general combiner for days to 50 per ce_ flowering, days to first picking and pods per raceme. Females NV-96 and NV-58 for pod yield per plant, average pod weight, seeds per pod and I pod length. The crosses like NY -96 x NY -40 showing high heterosis for yield and related components included at least one parent with high gca effect. On the other hand, significant association was observed between sea effect and hybrid performance for all the characters. In general, bi-parental mating and dialle} selective mating system could be advocated for accumulation of additive gene effect and breaking up undesirable linkages in' order to exploit both additive and non-additive genetic components.ThesisItem Open Access Line x tester Analysis in Sorghum (Sorghum bicolor (L.) Moench)(Agricultural Botany Department, N. M. College of Agriculture Gujarat Agricultural University, 2004-03) Desai, Niravkumar Janakbhai; Desai, N. JA field experiment was conducted during rabi season of 2004 at College Farm, Navsari Agricultural University, Navsari to study the "Response of rabi greengram [Vigna radiata (L.)l to irrigation, phosphorus and bio-fertilizer under South Gujarat condition." The soil of the experimental field was clay in texture having medium to poor drainage, low in total nitrogen (0.043 per cent), medium in phosphorus (32.35 kg ha'l) and fairly rich in potash (352.0 kg ha'l) with 7.8 pH. Eighteen trea~ment combinations consisting of three levels of irrigation viz., 0.3 IW/CPE ratio (Id, 0.5 IW/CPE ratio (h) and 0.7 IW/CPE ratio (13) with 6 cm depth of irrigation at each irrigation, three levels of phosphorus viz.: 20 kg P20 S ha'i (Pd, 40 kg' P20 5 ha'i (P2) and 60 kg P205 ha'i (P3) and two levels of bio-fertilizer namely control (Bo) and PSB inoculation (B 1) were tried in a factorial randomized block design with three replications. Seed and stover yields as well as most of the growth and yield attributes, quality of greengram seed, nutrients (N and P) content and uptake by seed, stover as well as total were significantly influenced by irrigation levels. In most of the character, treatment 0.7 IW/CPE ratio (I,) showed its superiority over treatment 0.3 IW/CPE ratio (Ill. Irrigating the crop at an IW/CPE ratio of 0.7 (I,) recorded significantly the highest seed (1045.6 kg ha· 1 ) and stover (2110.6 kg ha· 1 ) yields of greengram. These were 38.43 and 20.04 per cent higher over 0.3 (Ill and 0.5 IW/CPE (12) ratio, respectively. The consumptive use of water was increased with increase in levels of irrigation (I,) whereas consumptive water use efficiency was recorded higher with 0.3 IW/CPE ratio (Id. The highest net return (Rs.18435 ha· 1 ) with BCR value (5.01) was obtained under treatment 0.7 IW/CPE ratio (13). Among the levels of phosphorus, the highest level of 60 kg P20 S ha· 1 (P 3) out rightly dominated and established its superiority in respect to almost all growth and yield attributes. Application of 60 kg P20 S ha· 1 (P,) produced significantly the highest seed (1028.9 kg ha· 1 ) and stover (\978.5 kg ha· 1 ) yields. The per cent increase in seed yield with 60 kg P20 S ha· 1 (P3) were 17.23 and 34.43 per cent over treatment of 40 kg P20S ha· 1 (P2) and 20 kg P20 S ha· 1 (Pd, respectively. Crop fertilized with 60 kg P20 S ha· 1 (P3) recorded significantly higher protein content in seed as compared to rest of the lower levels. Similarly, 60 kg P20 S ha· 1 (P3) also recorded the highest nutrients (N and P) content and uptake by seed, stover and its total. The highestThesisItem Open Access A Study of Heterosis in Interspecific Crosses of Asiatic Cotton (G. herbaceum L. G. arboreum L.)(Botany Department, N. M. College of Agriculture, 1977-02) Raghnathji, Naik Maheshkumar; Patel, C. P.ThesisItem Open Access Genetic Variability and Correlation Coefficients for Yield and Other Quantitative Characters in Sorghum (sorghum bicolor L. Moench)(Botany Department, N. M. College of Agriculture, 1974-06) Metha, Harilal Dahyabhai; Desai, K. B.ThesisItem Open Access Genetic variability and Correlation Coefficients for Yield and Other Quantitative Characters in Ragi (Eleusine coracana Gaertn)(Botany Department, N. M. College of Agriculture, 1975-08) Agalodiya, Abbasbhai Valibhai; Desai, K. BThesisItem Open Access Study of Correlation Coefficients, variability and heritability in Some Types of Soybeans (Glycine max (L.) Merrill)(Botany Department, N. M. College of Agriculture, 1971-06) Chaudhari, Virsangbhai PathubhaiThesisItem Open Access Genetic variability Correlation and Path Analysis in Rice (Oryza sativa L.)(Agricultural Botany Department, N. M. College of Agriculture Gujarat Agricultural University, 1989-09) Patel, Sunil G.; Prajapati, R.M.