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Trematodes of Paramphistomatidae Infecting Domestic Ruminants
(Department of Parasitology, College of Veterinary and Animal Sciences, 1987) Tarun Shankar, Nath; KAU; George Varghese, C
The thesis embodied the results of an investigation on the prevalence of amphistome infection and their specific identity in different domestic ruminants (cattle, buffaloes, sheep and goats) of Kerala state. A total of 1490 faecal samples from domestic ruminants were collected from different places of Kerala State during a period from April 1985 to March 1986. These samples were examined by sedimentation technique to detect infection with amphistomes and their prevalence. Viscera of 780 slaughtered/ dead ruminants from different parts of Kerala were examined in addition and the available amphistomes were collected for the study and specific identification. The flukes were studied alive, flattened and stained and in certain cases by microtomy sections. Result of the study indicated that the prevalence of infection was far more in cattle and buffaloes than in sheep and goats. The rate of prevalence in cattle, buffaloes and goats was 20.16%, 28.57% and 3.22% respectively. In slaughtered/dead animals the prevalence was 33.09%, 34.67%, 4.17% and 5.81% respectively in cattle, buffaloes, sheep and goats. The highest prevalence was recorded during the rainy season and lowest in dry/summer season. Prevalence during south west monsoon was 38.08% and 20.73% during North East monsoon. The seasonal prevalence hardly varied between cattle and buffaloes but it was consistently low in sheep and goats. Most of the prevalence in all animals were of mixed origin. A total number of 17 species belonging to eight genera of amphistomes were identified. Cevlonocotyle spinicephalus (Tandon, 1955) was recorded from new hosts i.e, cattle and goats, cotylophoron chauhani from goats, in addition to the already reported hosts, buffaloes and sheep respectively. The following conclusions are drawn on the basis of the results of this study: Amphistomes are most prevalent in cattle and buffaloes. Though the infection with amphistomes exists throughout the year, it is definitely more in monsoon seasons. Ceylonocotyle spinicephalus (Tandon, 1955) occurs in cattle and goats and cotylophoron chauhani (Gupta and Gupta, 1972 ) in goats also.
Open Access
Immunodiagnosis of bovine gastrothylacosis using coproantigens
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 2000) Kandasamy, A; KAU; Devada, K
A study was conducted on the prevalence of paramphistomatidosis in Thrissur from June 1999 to May 2000, feasibility of coproantigen detection by ELlSA and comparison of sensitivity of ELlSA using coproantigens and ELlSA using serum antibodies in diagnosis of gastrothylacosis, caused by Gastrothy/ax crumenifer, in cattle. It was noted from the registers maintained at the University Veterinary Hospitals at Kokkalai and Mannuthy and that at the Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, that out of a total number of 1534 faecal samples from bovines examined, 253 (16.5 per cent) animals were found to be positive for amphistome eggs with the maximum prevalence (23 per cent) in June and July. Generally the infection was prevalent throughout the year. An indirect ELlSA using rabbit hyperimmune serum against somatic antigens of G. crumenifer was performed to detect coproantigens in faecal samples collected from 100 known G. crumenifer infected cattle. Seventy four samples were found to contain detectable levels of coproantigen indicating a sensitivity of 74 per cent. Serum samples collected from the same infected cattle were tested for antibodies to G. crumenifer by an indirect ELlSA using somatic antigens. Fifty one samples were found positive for antibodies indicating a sensitivity of 51 per cent. It was seen that when 43 cattle were positive for both coproantigens and serum antibodies, 18 cattle were negative for both of them. Although 31 cattle which were negative for serum antibodies were found positive for coproantigens, eight cattle negative for coproantigens were found positive for serum antibodies. The results showed that coproantigen detection, which revealed a higher sensitivity than the detection of serum antibodies by ELlSA, is feasible for the diagnosis of gastrothylacosis in bovines.
Open Access
Studies on certain gastro-intestinal nematodes with special reference to those found in goats
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sathianesan, V; KAU; Kalyanasundaram, R
Life history of Triohuris globuloss has been elucidated for the fist time using kids !q as experimental animals. Both free living and parasitic stages have been described in detail with illustrations. The prepatent period of Triohuric globuloss was found to be 85-89 days. T.globulosa failed to develop in guniea pig on experimental transmission. Haematology and histopathology of trichuriacis in kids experimentally infected with T.globulosa have been studied for the first time and heavy infection with the worm was found to cause anaemia and pathology changes in cacum of the host. Prepatent period of Strongyloides napillosus in kids infected orally with 7000 larva has been determined for the first time to be 6-7 days. Neoasoais vitulorum of calves has been found to be not transferable to oat either transplacentally or orally. In gunia pigs M.Vitulorum failed to attain sexual maturity. Calves could not be infected with infected with M.vitulorum post-nataly. Oesphagostomum colubianum of goats has been found to be not transferable to calves. Prepatent period of O.colusbionum in kids infected experimentally was found to be 40 days. Comparative efficacy of 4 anthelminties viz., Oxibendazole, thiophanate, Albendazole and Parbendazole was increased by conducting treatment trials. Against monospecific infection of Trichuris globulosa in experimentally infected kids the efficacies of Oxibendazile, thiophanate, Albendazole and Parbendazole at the recommended doses of 10mg.50mg, 10mg and 30 mg/kg body weight respectively were found to be 48.6%, 41%,47.3% and 49.3% respectively. Even at double the recommended doses the anthelminations were found to be well tolerated by the kids. Oxibendazole and Albendazole at the rate of 10mg and 10mg/kg body weight respectively were found to be 100% found to be 100 % effective against colubriformise, effective against respectively were Haemonohus contortue, trichostrongylus .Thiopanate at the recommended dosage (59mg/kg body weight) was also 100% effective against all he nematodes mentioned above except Haemonous contortus against which it was only 97% effective at that dosage. Parbendazole at the recommended dosage shedule (30mg/kg body weight) was 100% effective against the nematodes with the exception of .against which only 86.6% efficacy was noticed. Against in nature nematodes in the gastro-intestinal tract Oxibendazole and Albendazole were 100% effective whereas the efficacies of P`arbendazole and thiophanate were 85% and 74% respectively at their recommended doses. In in vitro studies the anthelmintic efficacy of Oxibendasole against Haemonchus contortus was found to be superior to that of the remaining anthelminties. In ovicidal property also, Oxibendazole was better than all the others, since it was ovicidal in 6 hours after administration whereas albendazole, thiophanate and Parbendazole were ovicidal in 8 hours, 9 hour and 11 hours respectively. Albendazole was found to be superior to al the other 3 anthelmintion in their influences on the body weight gain of treated animals closely followed by Oxibendazole. Perbendazole and thiophanate were inferior to others Judging on the basis of over all efficacies Oxibendazole and Albendazole were found to be superior to thiophanate and Parbendasole. Treatment trials with Oxibendazole, Albendazole and thiophanate against gastro-intestinal nematodes of goats have not been attempted earlier than the present study.
Open Access
Studies on the pathogenicity of tetrameres mohtedai and acuaria spiralis of fowl
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1977) Ramaswamy, K; KAU; Sundaram, R K
Controlled experiments were conducted at three levels of monospecific and dispecific infections with two commonly occurring spirurid worms of fowls viz. Tetrameres mohtedai and Acuaria spiralis. The blood studies conducted in monospecific T. mohtedai infection revealed a normocytic normochromic anaemia and eosinophilia during the initial phase and later a macrocytic anaemia, heterophilia and slight monocytosis by 66th day post infection. The infected birds lost considerable amount of their body weight. In layers there was a great delay in sexual maturity and poor feed efficiency as indicated by greatly reduced egg production. The percentage of establishment of worms in these experiments were found to be inversely proportional to the number of juveniles administered. The pathological changes in the affected proventriculi indicated an acute inflammation during the invasive phase of juveniles, followed by a chronic reaction which by 55th day post infection was well established indicating the development of a host – parasite balance towards the later stages of infection. Among the blood changes in monospecific A. spiralis infection, eosinophilia was evident initially followed by a marked leucopenia and development of a macrocytic hypochromic anaemia. Other effects due to parasitism were, delayed maturity, reduced rate of egg production, poor feed efficiency, loss of weight, emaciation and a mortality rate of 25% in chicks. The gross pathological changes in Acuariasis included the appearance of an irregular whitish patch on the serosa and formation of nodular growths in the mucosa which in heavy infections occupy the whole of the mucous membrane. Juveniles of A. spiralis were found to invade the compound glands in very heavy infections. The microscopic changes indicate a severe acute inflammation during the initial stages. Later as the disease became chronic pedunculated fibro – adenamatoid growths were evident on the mucous membrane. In dispecific infections with T. mohtedai and A. spiralis the haematological changes observed were eosinophilia during the early stages followed by a l eucopenia and heterophilia. As the disease progressed a macrocytic hypochromic anaemia developed. There was a great decrease in the live body weight of the infected birds. A mortality of 33.33% were observed in chicks with heavy infection. In layers the sexual maturity was delayed and egg production was greatly suppressed with consequent poor feed utilization. The gross and microscopic changes found in the affected proventriculi indicated a combination of lesions observed under monospecific infections of both the parasites. The results obtained show that the loss due to morbidity (mainly as a result of decreased body weight and reduced rate of egg production) was considerable. These facts indicate that both T. mohtedai and A. spiralis are economically important.
Open Access
Studies on the biology, pathogenicity and treatment of important nematodes of domestic duck
(Department of Parasitology, College of Veterinary and Animal Sciences,Mannuthy, 1977) Chandrasekharan, K; KAU; Kalyanasundaram, R
Studies on the incidence of nematode infections in 340 domestic ducks indicated that 67.94% of birds harboured one or more species of the following nematodes viz., Amidostomum globocaudata skrjabini , capillaria contorta, capillaria , Echinuria uncinate, Epomidiostum uncinatum, Eustrongylides papilosus, Strongyloides avium and tetramers antis. The rate of infection was highest in the case of Epomidiostomum uncinatum (41.86% ) and lowest in the case of strongyloides avium (0.59%). The lifecycles of Echinuria uncinata and Tetramers anatis were worked out in detail. It was established that Tetramers anatis was distinct from Tetramers Fissispina. The complete details of the morphology of juveniles both within the intermediate and final hosts were given. The juveniles of Echinuria uncinata completed the first and second moultings on the third and fifth day respectively and the stage juveiles reached infective stage on the seventh day following infection in Daphnia pulex at a room temperature ranging from 25 to 30C0. In ducklings the juveniles underwent the third and fourth moultings on the third and seventh day respectively and the eggs of the parasite were first found in the droppings on the thirty- third day. The juveniles of Tetramers anatis completed first mopulting on the third day, the second moulting on the fifth day and the third stage encysted in fat bodies of the grasshopper, Spathosternum prasiniferum. The third stage juveniles attained infective stage on the sixth day following infection. Within the ducklings, the parasite underwent the third moulting on the third day. The fourth moulting in respect of males was seen on the nineth day and in the case of females on the tenth day. The prepatent period of the parasite was found to be 24 days. Development of the juveniles was also noticed in five other species of grasshoppers viz., Oxya nitidula, Oedaleus abruptus, Conocephalus maculatus, Ducetia Japonica and Atractomorpha crenulata. No development of Tetrameres anatis, beyound the first stage was observed inDaphnia Pulex and Daphnia Magna on experimental infection. Anaemic changes with significant reduction in weight gain and haematological values were observed in experimental infections of Tetrameres anatis, Amidostomum skrjabini and Epomidiostomum uncinatum. Histopathological changes like haemorrahage, desquamation of cells, catarrbal inflammation and necrosis were seen in experimental tetramerosis. Ulceration and atrophy were also evident in amidostomiasis and epomidiostomiasis. The anthelmintic efficacy of 9 drugs against adult worms of Tetrameres anatis, Amidostomum skrjabini and Epomidiostomum uncinatum under experimental infections were determined. The efficacies against tetramerosis, amidostomiasis and epomidiostomiasis were 79.23%,32.15% and 79.88% with Tetramisole by drochloride at 50 mg/kg body weight; 70.36%, 52.42% and 50.23% with phenothiazine at 500 mg/kg; 26.85%, 74.8% and 65.68% with Morantel tartrate at 40mg/kg; 45.70% 67.29% and 57.84% with Parbendazole at 100 mg/kg; 41,0%’63/93% and 43.13% with Thiabendazole at 200 mg/kg; 59.28%,61.06% and 52.66% with Carbontetrachloride at 2ml/kg; 35.94%, 30,12% and 13.13% with Methyridine at 200 mg/kg; 28.87% 20.17% and 11.32% with Disophenol at 10 mg/kg and 31.02%, 39.04% and 12.66% with Cashewnut shell oil at 10 g/kg body weight respeectively. The drug Rametin at 200 mg/kg was found to be fatal to ducklings. In a cross infection trial delyed development and lesser percentage of establishement of Ascaridia galli of fowl origin were observed in ducklings as compared to chicks.
Open Access
Helminth Parasites Of Indian Elephants
(Department of Parasitology, College of Veterinary and Animal Sciences,Mannuthy, 1988) Khomdram Somorendro, KAU; Chandrasekharan, K
The present study was conducted over a period of one year, during which dung samples from 203 Captive Indian elephants from different parts (urban, village and forests) of Kerala of different age and sex were examined. Out of this, 117 elephants were found to be infected with different hel - minth parasites either as pure or mixed infection. The overall prevalence of helminthiasis was 57.6 per cent. Helminthiasis was more during rainy season (70 per cent) than dry or summer season (52.44 per cent). Eleven species of adult nematodes and three species of adult amphistomes and eggs of Bivetellobilharzia nairi and Anoplocephala manubriata were recorded during this study. The nematode parasites were Murshidia murshidia, Murshidia falcifera, Quilonia renniei, Amira pileata, Choniangium epistomum, Equinubria sipunculiformis, Decrusia additictia, Grammocephalus hybridatus, Grammocephalus varedatus, Parabronema indicum, Parabronema smithi and the amphistomes were Pseudodiscus collinsi, Pseudodiscus hawkesii and Gastrodiscus secundus. The more common species were Murshidia murshidia and Murshidia falcifera. Anthelmintic efficacy of albendazole (Albomar) was assessed on the basis of the reduction of eggs in the dung samples. A single oral dosage of 2.5 and 3 mg per kg body weight was found 100 per cent effective against strongyle infection in naturally infected captive Indian elephants.
Open Access
Studies on the common ticks affecting live-stock in Kerala
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Rajamohan, K; KAU; Kalyanasundaram, R
An investigation was conducted for a period of 5 years from 1974 to 1979 in to the incidence of ticks on domestic animals in Kerala, the biology of the most common species, and their susceptibility to four commonly available organo phosphorus compounds.. Nine different species of ticks belonging to 5 genera were collected from cattle, buffaloes, goats and dogs in different localities of the eleven districts of Kerala. The species of ticks identified were boophilus annulatus, rhipicephalus sanguineus, R.haemaphusaloides, R.turanicus, haemaphysalis bispinosa, H. turturis, H.spinigera, Hyalomma anatolicum and Amblyomma integrum. The commonest among them was boophilus annulatus, which was found mainly on cattle. The incidence of this tick was very high in the hilly areas and in the western ghat regions. The other common ticks found on cattle were Rhipicephalus haemaphysaloides and Haemaphysalis bispinosa. Tick infestation was found to be rare in buffaloes and goats and the common species found on them were Haemaphysalis bispinosa and B. annulatus. IN dogs the species recorded in the present study were Rhipicephalus sanguienus and R. turanicus. Variations in incidence was evidenced from the present study. The maximum incidence was found in the months of July, August, September, October and November. The life-cycle of B.annulatus was completed experimentally in a period 44 days. The maximum number of eggs produced by one tick was 1420 and minimum 680. The incubation period was dependent on the room temperature and variation in humidity had little influence. The larvae were found to prefer hairy areas of the body for attachment, and they did not attach at the hairless and exposed areas. The engorged females were found to drop off quickly when green grass was spread on the floor of the experimental animal’s shed. The life-cycle patter could be summarised as: Pre-oviposition : 1 to 6 days Oviposition : 5 to 9 days Incubation period : 16 to 30 days Larval fasting : 2 to 5 days Larval feeding : 4 to 7 days Larval moulting : 8th to 12th day of infection Nymphal feeding : 3 to 6 days Moulting of Nymph : 15 to 19th day of infection Adult feeding : 5 to 10 days Detatchment of female : From 20th day of infection onwards Four organophosphorous compounds viz. Malathion, Fenitrothion, Dichlorvos and Phosalone were tested for their action on ticks. The ovicidal and larvicidal actions and the lethal effects on nymphas and engorged females of B. annulatus, R sanguinius and H. bispinosa were studied in the laboratory. Cythion (malathion 50%) 0.5 to 1%, Sumithion (Fenitrothion 50 %) 0.5 to 1%, Dichlorvos (Nuvan) 0.1 to 0.2%, and Zolone (Phosalone 35 %) 0.2 to 0.3% were found effective at different degrees. The efficacy was directly proportional to the concentration of compound and the duration of treatment. Phosalone showed maximum efficacy followed by Dichlorvos. In field trials, all the four compounds gave satisfactory results. But phosalone showed maximum efficacy and least toxicity to animals. Nuvan was also effective but was the most toxic among the four compounds. Thus the acaricide of choice for routine use in Kerala was determined to be Phosalone (35%) at a concentration of 0.3% to be used as a spray or wash.
Open Access
Effect of certain biopesticides and irradiation on the developmental stages of myiasis producing flies
(Department of Parasitology, College of Veterinary and Animal Sciences,Mannuthy, 1998) Subramanian, H; KAU; Rajamohanan, K
A study was undertaken on the prevalence of cutaneous myiasis in domestic animals and its control using bio-pesticides and gamma irradiation. The prevalence of cutaneous myiasis in domestic animals was found to be 205 (2.08 per cent) among .the 9861 animals screened. The peak of infestation was noted in the month of January. In host-wise and parasite-wise the highest incidence was noted in cattle (63.41 per cent) and the majority of infestation was produced by Chrysomya bezziana larvae (90.73 per cent). Methoprene at 1 to 50 ppm concentration caused only moderate mortality on larvae but significantly increased the mortality rate on eggs, prolonged the larval phase, increased the formation of larval pupal intermediaries and adult deformities and reduced the adult emergence. Diflubenzuron at 0.5 to 5 ppm caused 55 to 100 per cent larvicidal effect due to lowered chitin content of 18.42 to 52.11 per cent in larval cuticle. Bacillus thuringiensis var israelensis produced only moderate larval mortality at 160 to 800 ppm in myiasis producing flies. Azadirachtin at 10.5 to 15 ppm produced 100 per cent mortality in eggs and larvae. Significant antifeedant, ovipositional deterrent and repellant effects were also produced by Azadirachtin. Among the extracts of Acarus calamus, studied, petroleum ether extract at 2.5 per cent concentration gave 82.5 to 100 per cent mortality of the larvae. Moderate antifeedant, ovipositional deterrent and repellant effects were also noticed. The petroleum ether extract produced 100 per cent sterility at 0.1 to 1 per cent concentration by preventing the development of ovarian follicles. Three day old pupae of myiasis producing flies exposed to r-rays gave excellent sterility effect at 2000 to 4000 rads radiation exposure without any other deleterious effect in the flies. Diflubenzuron at 5 ppm concentration showed the highest larvicidal effect (88.5 per cent) in natural cases of cutaneous myiasis.
Open Access
Trematodes of paramphistomatidae infecting domestic ruminants
(Department of Parasitology, College of Veterinary and Animal Sciences,Mannuthy, 1987) Tarun Shankar Nath, KAU; George Varghese, C
The thesis embodied the results of an investigation on the prevalence of amphistome infection and their specific identity in different domestic ruminants (cattle, buffaloes, sheep and goats) of Kerala state. A total of 1490 faecal samples from domestic ruminants were collected from different places of Kerala State during a period from April 1985 to March 1986. These samples were examined by sedimentation technique to detect infection with amphistomes and their prevalence. Viscera of 780 slaughtered/ dead ruminants from different parts of Kerala were examined in addition and the available amphistomes were collected for the study and specific identification. The flukes were studied alive, flattened and stained and in certain cases by microtomy sections. Result of the study indicated that the prevalence of infection was far more in cattle and buffaloes than in sheep and goats. The rate of prevalence in cattle, buffaloes and goats was 20.16%, 28.57% and 3.22% respectively. In slaughtered/dead animals the prevalence was 33.09%, 34.67%, 4.17% and 5.81% respectively in cattle, buffaloes, sheep and goats. The highest prevalence was recorded during the rainy season and lowest in dry/summer season. Prevalence during south west monsoon was 38.08% and 20.73% during North East monsoon. The seasonal prevalence hardly varied between cattle and buffaloes but it was consistently low in sheep and goats. Most of the prevalence in all animals were of mixed origin. A total number of 17 species belonging to eight genera of amphistomes were identified. Cevlonocotyle spinicephalus (Tandon, 1955) was recorded from new hosts i.e, cattle and goats, cotylophoron chauhani from goats, in addition to the already reported hosts, buffaloes and sheep respectively. The following conclusions are drawn on the basis of the results of this study: Amphistomes are most prevalent in cattle and buffaloes. Though the infection with amphistomes exists throughout the year, it is definitely more in monsoon seasons. Ceylonocotyle spinicephalus (Tandon, 1955) occurs in cattle and goats and cotylophoron chauhani (Gupta and Gupta, 1972 ) in goats also.

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