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ThesisItem Open Access Controlling seedling height of cowpea (Vigna unguiculata (L.) Walp.) transplants using plant growth regulators(Department of Vegetable Science, College of Agriculture, Vellanikkara, 2022-04-28) Anu Latha Robi; KAU; C NarayanankuttyCowpea (Vigna unguiculata (L.) Walp) is an annual leguminous vegetable crop. It is grown throughout india for its tender green pods used as vegetable. Green cowpea seeds are boiled as a fresh vegetable or may be canned or frozen. The beans are nutritious and provide complementary proteins to cereals diets. Seedlings like cowpea, cucurbits having lanky growth growing in polyhouses is an undesirable quality, especially in plugs intended for mechanized planting and long distance transport. Seed treatment with growth retardants is an effective method for controlling seedling height .However limited studies have been conducted in vegetable crops The present study was undertaken with the objectives of controlling seedling height of vegetable cowpea and to assess the field performance of treated seedlings. The experiment was conducted at the Centre for Hi-Tech Horticulture and Precision farming, Vellanikkara KAU, Thrissur during the year 2020-2021 In seedling stage, highest germination was recorded in control treatment on 5th and 10th days after sowing (89%) and (91%) respectively in Vellayani Jyothika. Minimum germination percentage was recorded in Paclobutrazol @ 200 ppm on 5th and 10th day after sowing (64.3%) and (66.0%) respectively in cowpea genotype Vellayani Jyothika. Significantly lower germination percentage was recorded in treatments receiving Paclobutrazol @ 200 ppm on 5th and 10th day after sowing (70.0%) (72%) in the genotype Kashi Kanchan. In both the genotypes there were no significant difference between the treatments on 15th days after sowing. Paclobutrazol treatment took more number of days for emergence of seedling up to the first node compared to control treatment ie Paclobutrazol @ 200 ppm (8.13) and (8.00) respectively in both the genotypes. Highest shoot length was recorded in control treatment on 5th, 10th and 15 th days after germination in both the genotypes. Significantly lower shoot length was recorded in treatments receiving Paclobutrazol @ 200 ppm irrespective of the number of days after germination. Growth retardants in general reduce root growth, shoot weight and root weight of the seedlings. In both the genotypes (Vellayani Jyothika and Kashi Kanchan) significantly lower root length, shoot weight and root weight was obtained in Paclobutrazol treatments compared to non-treated control. Height of seedling up to the first node was directly linked to shoot length. In both the genotypes of the cowpea significantly lower shoot length was recorded in treatments receiving Paclobutrazol @ 200 ppm (4.09) and (4.03) respectively. Paclobutrazol treated seedlings had dark green leaves when compared to other growth regulator and control treatments on 5th ,10th and 15th days after germination. In both the cowpea genotypes, when compared to other growth retardant and control treatments highest stem girth of the seedlings was recorded in treatments receiving Paclobutrazol @ 200 ppm on 5th (3.19 mm) 10th (3.45mm) and 15th (3.7mm) in Vellayani Jyothika and on 5th (3.3mm ) 10th (3.4mm) and 15th (3.8mm) in Kashi Kanchan. There were no significant difference between the treatments on 5th (2) and 15th days (7-8) after germination in both the genotypes. The total chlorophyll content and carotenoid content was recorded highest in treatments receiving Paclobutrazol treatments on seedling stage. Minimum auxin content and gibberellic acid contents were observed in Paclobutrazol treatments. In the field experiment, in both the cowpea genotypes, plant growth regulator Ethrel recorded maximum plant height where as in Vellayani Jyothika minimum plant height was recorded in Cycocel@ 50 ppm (112.40 cm) .In Kashi Kanchan lowest plant height was recorded in Paclobutrazol @ 200 ppm (33.06 cm) in Kashi Kanchan. In vellayani Jyothika highest internodal length was recorded in Ethrel @100 ppm (3.13 cm) and Ethrel @50 ppm (3.04 cm). Minimum was recorded in Paclobutrazol@ 200 ppm (2.63 cm). In the genotype Kashi Kanchan maximum internodal length was recorded in Ethrel@ 200 ppm (3.34 cm) and minimum was recorded in Cycocel @ 100 ppm (2.53cm). Highest number of primary branches was recorded in treatments receiving Paclobutrazol @ 200 ppm which was on par with Paclobutrazol @ 100 ppm compared to control in Vellayani Jyothika. In Kashi Kanchan there were no significant differences found between the treatments. Growth retardants had significant effect on the viz days to first flowering, days to first harvest and crop duration. Early flowering results in early harvesting of pods. Plant growth retardant Cycocel @ 200 ppm (41day) and Cycocel @ 25 ppm (36.80 days) recorded earliest flowering in the field in both the genotypes. In both the genotypes delayed flowering and maximum crop duration was recorded in Paclobutrazol treatments. Pod length and pod girth are related with pod weight. Ethrel treatment had highest pod length and pod girth compared to control .The higher number of flowers, pod girth and pod length contributed to production of more number of pods which in turn resulted in higher yield per plant. In both the cowpea genotypes, highest number of pods per plant and yield per plant was recorded in Ethrel treatments (Ethrel 100 ppm) in Vellayani Jyothika followed by Paclobutrazol treatment (Paclobutrazol 100 ppm) where as in Kashi Kanchan Paclobutrazol 100 ppm was on par with Ethrel 100 ppm. From the above study it could be concluded that, Paclobutrazol 100 ppm seed treatment was effective in controlling seedling height as well to obtain good yield in vegetable cowpea.ThesisItem Open Access Breeding yard long bean(Vigna unguiculata subsp.sesquipedalis (L.) Verdcourt) for anthracnose resistance through conventional and molecular marker analysis(Department of Vegetable Science College of Agriculture ,Vellayani, 2022) Merin Elza George; Sarada,SThe study entitled ―Breeding yard long bean (Vigna unguiculata subsp. sesquipedalis (L.) Verdcourt) for anthracnose resistance through conventional and molecular marker analysis‖ was carried out at the Department of Vegetable Science, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, during 2019-2022. The objective of the study was to identify molecular marker(s) linked with anthracnose disease resistance in the F2 segregating population of yard long bean, using bulk segregant analysis to enable marker assisted selection along with conventional breeding. The study was undertaken in six experiments. In experiment I, 50 genotypes of vegetable cowpea collected from different parts of India, including the released varieties of SAUs and ICAR institutes were screened against the most virulent isolate of Colletotricum gloeosporioides through artificial inoculation. Among the 50 genotypes tested, VU 53, with semi-trailing growth habit was found to be highly resistant with disease severity of 3.67 ± 0.13 %. Three susceptible, high yielding long podded varieties viz., KAU Deepika, Vellayani Jyothika and Githika, selected as female parents were crossed with VU 53, the most resistant genotype as male parent for the development of three hybrids. In experiment II, two crossing blocks were laid to produce the seeds of three hybrids and their F2 progenies. The three hybrids were evaluated along with the parents under replicated trial in the main field for trailing nature, yield and quality in experiment III. Significant variation was noticed on mean performance of yard long bean parents and hybrids for most of the characters studied. Earliest flowering was observed in KAU Deepika x VU 53 (57.33 ± 0.57 days) and delayed flowering in Githika x VU 53 (71.66 ± 1.15 days). Among the hybrids, the highest pod length was recorded in KAU Deepika x VU 53 (24.50 ± 1.00 cm), while the Vellayani Jyothika x VU 53 had the maximum pod girth (2.90 ± 0.10 cm). Maximum pod weight and pods per plant was recorded in KAU Deepika x VU 53 (19.22 ± 0.58 g and 79.33 ± 0.59 respectively) among hybrids. The cross Vellayani Jyothika x VU 53 (16.55 ± 0.20 g) exhibited maximum seed weight. Among the three hybrids, KAU Deepika x VU 53 (1416.00 ± 126.09 g) recorded the highest yield per plant and was on par with Githika x VU 53 (1343.33 ± 75.06 g). Among the hybrids, KAU Deepika x VU 53 (67.85 ± 0.96 days) was significantly superior for days to harvest. The longest duration among hybrids was observed in KAU Deepika x VU 53 (126.56 ± 0.37 days) and the shortest duration was recorded in Vellayani Jyothika x VU 53 (121.31 ± 0.58 days). High heritability and genetic advance was recorded for vine length, days to first flowering, pod length, pod weight, pods per plant, yield per plant, yield per plot, hundred seed weight and keeping quality. Yield per plant had significant positive phenotypic and genotypic correlation with pod weight, followed by pods per plant, length of terminal leaf, seeds per pod, pod length, crop duration, keeping quality and days to harvest. Pods per plant could be considered as the most important yield trait for enhancing the yield in yard long bean because of its high, positive correlation and direct effect with yield per plant along with high heritability and genetic advance. In experiment IV, parents, three hybrids and F2 progenies were evaluated for resistance to natural incidence of C. gloeosporioides in the main field, as unreplicated trial and without any plant protection measures. All the three F1 were found to be field tolerant. Out of 175 F2 plants of the cross KAU Deepika x VU 53, 164 plants were susceptible and 11 were resistant. In the case of Vellayani Jyothika x VU 53, 175 plants were susceptible among the 187 individual F2 progenies observed. Similarly, in the cross Githika x VU 53, 188 plants were susceptible while 12 plants were found to be symptomless. The F2 progenies of the high yielding hybrid KAU Deepika x VU 53 were evaluated for resistance to C. gloeosporioides through artificial inoculation in experiment V. Out of 196 plants, 185 plants were found to be susceptible whereas 11 plants were resistant which could be utilized in marker assisted breeding programme. The segregation for anthracnose resistance in F2 plants was in the ratio 15:1 (185 susceptible plants out of 196), which suggests that the inheritance of anthracnose resistance may be controlled by two recessive genes. Bulked segregant analysis was done using the DNA from the resistant parent, susceptible parent, resistant F2 bulk and susceptible F2 bulk in experiment VI. Bulked segregant analysis with the SSR primer CLM0008 revealed a polymorphic band at ≈ 300 bp which was appeared in the resistant parent and bulk. Bulked segregant analysis with ISSR primer UBC 811 produced polymorphic bands at ≈ 1100 bp, which were present in resistant parent and resistant bulks. The identified markers linked with resistance were validated over the resistant F2 lines obtained.ThesisItem Open Access Standardization of technology for microgreen production(Department of Vegetable Science, College of Agriculture, Vellanikkara, 2021) Arya, K S; KAU; Sangeetha Kutty, MMicrogreens are new class of vegetables that are gaining popularity in the recent years due to their attractive appearance coupled with vivid flavors. They are young immature greens produced from seeds of vegetables or herbs which are harvested at its true leaf stage. Most of the research works in this area are carried out with temperate species of crops whose seeds are very expensive. Hence there is a need to popularize the microgreens of tropical species at an affordable price with minimum inputs, so that it is easily accessible to common people. The study entitled “Standardization of technology for microgreen production” was conducted at the Department of Vegetable Science, College of Agriculture, Vellanikkara during January to October 2021. The study was experimented with wheat, ragi, green gram, horse gram, amaranthus and mustard whose seeds were collected from the local market. It was conducted as four experiments (standardization of seed treatment, standardization of media, standardization of seed density and to assess the nutritional value and yield under different growing conditions). The first experiment was to standardize seed treatment done using two chemicals hydrogen peroxide and vinegar treated at different concentrations. The observations on germination percentage, seedling vigour and yield were recorded. The seed treatment did not show any effect on growth of microgreens when compared to the control on the parameters recorded. The microscopic observation of freshly harvested microgreens for fungal contamination indicated absence of any contamination in the fresh microgreens. The experiment on standardizing growing media was carried out using five media viz, sterile sand, cocopeat, coir mat, tissue paper and newspaper. The observation on yield, seedling height, nutritional value of microgreens and microscopic observation on fungal growth were recorded. Yield was observed to be highest in microgreens grown on cocopeat media and lowest was recorded in microgreen grown on newspaper media and among the crops it was highest for green gram microgreens (1.03 to 1.49 kg/m2). The seedling height showed a slight decrease in ragi and mustard microgreens grown on newspaper and comparing the crops, it was observed to be maximum in green gram microgreens (10.23- 10.54 cm). The nutritional parameters recorded were not showing any difference when sown in different media. The wheat microgreens were observed to rich source of vitamin C, beta carotene and protein, ragi microgreens were showing more calcium and vitamin C and beta carotene content, green gram microgreen were rich in protein, vitamin C and calcium, horse gram microgreens were rich in vitamin C, chlorophyll, beta carotene and crude protein, amaranthus microgreens possess high iron, calcium, beta carotene and vitamin C content and mustard microgreens were rich in protein and beta carotene, iron and vitamin C. Microscopic observation on freshly harvested produce revealed that there is absence of fungal contamination in microgreens. The seed density for microgreen production was standardized in the third experiment. The seeds were sown at three densities viz, low, medium and high density for each crop. The observations recorded included yield, seedling height and microscopic observation on fungal growth. The yield was recorded to be highest when sown at high density for wheat (705 g seeds/m2), ragi (520g seeds/m2) and amaranthus(120g seeds/m2) microgreens and medium density planting yielded highest for green gram (850g seeds/m2), horse gram (617 g seeds/m2) and mustard (440 g seeds/m2) microgreens. The seedling height recorded was observed to be on par except for green gram and horse gram microgreens, where highest seedling height was found in medium density planting. No fungal contamination was observed in microscopic observation of fresh produce. Nutritional value and yield of microgreens grown under two conditions (room condition and rain shelter) were studied in fourth experiment. The parameters recorded were yield, seedling height, nutrient content under two conditions, organoleptic evaluation and shelf life of microgreens. The yield was observed to be significantly high when raised under rain shelter condition than room condition. Among the crops it was highest for green gram microgreens (2.261 kg/m2). The seedling height was observed to reduce under rain shelter condition. Nutritional content was also observed to be high when planted under rain shelter for all parameters viz, iron, calcium, beta carotene, vitamin C, crude protein, crude fibre, chlorophyll, oxalates and nitrates. Organoleptic evaluation revealed highest total mean rank for mustard microgreens (54.56) raised under room condition. The shelf life study revealed that the storage period of microgreens can be extended when it is stored in ziplock PPE bag under low temperature condition. In this study, several aspects of microgreens production viz, seed treatment, growing media, seed density, growth conditions and shelf life were standardized. The results indicate that microgreen cultivation can be recommended both as a commercial and household venture.ThesisItem Open Access Standardisation of operational procedures for pro-tray seedling production of vegetables(Department of Vegetable Science, College of Agriculture, Vellanikkara, 2022) Arya, S; KAU; Dicto Jose, MHealthy seedlings are the most important prerequisite for any vegetable crop to reach its full output potential. As a result, large-scale seedling production is becoming a profession and a commercial activity. Farmers used to generate seedlings at a reasonable cost in nursery beds in the past. However, due to some drawbacks like poor germination, higher pest and disease incidence, poor field establishment etc. in traditional nurseries, many progressive farmers and agriculture entrepreneurs have turned their attention to the commercial production of quality seedlings utilising seedling trays or pro-trays. In this background, the present study entitled ‘Standardisation of operational procedures for pro-tray seedling production of vegetables’ was undertaken with the objective of standardising best growing media, pre-sowing seed invigoration, and evaluating the effect of biofertilizer on pro-tray seedling production of vegetables. The present study was carried out at the Department of Vegetable Science, College of Agriculture, Vellanikkara as three experiments during 2020-21. All three experiments were conducted in a Completely Randomized Design with three replications. In the first experiment seeds of tomato var. Anagha, chilli var. Anugraha, cucumber var. Heera, and okra var. Salkeerthi collected from the Department of Vegetable Science were sown in seventeen different media that were initially analysed for various physicochemical properties. The growth parameters of the seedlings raised in the poly house were assessed at the stage of commercial sale of each crop i.e., 15 days after sowing (DAS) in cucumber and okra and 30 DAS in chilli and tomato. The results showed that T13 (cocopeat: vermiculite: perlite - 1:1:1) and T17 recorded comparatively minimum bulk density, maximum water holding capacity and porosity which are desirable physical properties for good seedling growth. T17 (coir pith compost: vermiculite: perlite - 3:1:1) was found to be the best soil-less media for all four crops based on growth parameters and cost analysis. Among soil containing media T6 having soil and dried powdered cow dung in equal proportion and T10 having soil, cocopeat and dried cow dung exhibited a higher benefit cost ratio and was found to be on par with T17 for most of the growth parameters. Farmers do not have easy access to vermiculite and perlite, and they are costly. In this context, soil, cocopeat and dried powdered cow dung in equal proportion is found to be an alternate media comprising locally available inputs and affordable by the farmers. The best media from the first experiment was chosen for further trials. The second trial comprised of nine different seed treatments including various concentrations of KNO3 and Pseudomonas. Pre-sowing seed invigoration with 0.5% KNO3 in combination with 1% Pseudomonas was found to be highly effective in improving germination and growth parameters viz., germination percentage, plant height, root length, vigour index Ⅰ & Ⅱ and chlorophyll content of seedlings of tomato, chilli, cucumber and okra. This combination has the dual advantage of providing potassium and nitrogen to the seedlings and imparting resistance from diseases especially damping off. So, these treatments can be effectively utilized for producing healthy and vigorous vegetable seedlings. The third experiment included nine treatments in which different biofertilizers viz., PGPR Mix-1, Pseudomonas fluorescense, Trichoderma asperellum and arbuscular mycorrhizal fungi were inoculated in the media individually and in combination. Evaluation of the effect of biofertilizers showed that higher growth parameters like plant height, root length, leaf area, collar girth, vigour index Ⅰ and vigour index Ⅱ are comparatively higher in the treatment containing PGPR, AMF and along with either Pseudomonas or Trichoderma. In the above three experiments the best treatments were identified by ranking based on an index derived from principal component analysis. The characters viz., number of leaves, leaf area, collar girth, vigour index Ⅰ & Ⅱ and total chlorophyll content was used for performing PCA. The loadings of principal component having Eigen value more than one was taken as weightage. Using weightage, an index was derived and the treatments having higher index was ranked higher. The best media obtained include cocopeat: vermiculite: perlite (3:1:1) and soil: cocopeat: cow dung (1:1:1). A combination of 0.5% KNO3 and 1% Pseudomonas was the best seed invigoration treatment. Among different biofertilizers evaluated the combination of AMF, PGPR Mix-1 and Pseudomonas was found to be the best. Based on the insights of the above three trials, standard operational protocol for seedling production of tomato, chilli, cucumber and okra is generated for use by farmers.ThesisItem Open Access Evaluation of bush dolichos bean [Lablab purpureus (L.) sweet] for yield and quality(Department of Vegetable Science, College of Agriculture, Vellayani, 2021) Maneesha, P K; KAU; Sarada, SThe present investigation entitled “Evaluation of bush dolichos bean [Lablab purpureus (L.) Sweet] for yield and quality” was conducted at the Department of Vegetable Science, College of Agriculture, Vellayani, from June 2019- December 2020 to evaluate the adaptability of bush dolichos bean in Kerala based on growth, yield and quality. The experimental material consisted of 25 bush dolichos bean genotypes. The experiment was laid out in RBD with two replications. Analysis of variance revealed significant differences among the twenty five genotypes for all the characters studied. Among the genotypes, HA- 4 recorded the highest plant height of 65.75 cm. The variety HA-3 recorded the highest plant spread of 48.95 cm. The highest number of primary branches plant-1 was recorded by HA-4 (5.70), Arka Jay (5.65) and HA-3 (5.55) were on par with it. HA-4 recorded the highest leaf area of 13.35 cm2. Earliest flowering was observed in the genotype VRBSEM- 15 (22.95 days), while first flower appearance was late in HA- 4 (67.00 days). Fifty percent flowering was earliest in VRBSEM- 15 (25.95 days). The highest number of racemes plant-1 was recorded in HA-4 (6.46) and the longest raceme in the variety Arka Jay (34.35 cm). Arka Sambram was the earliest to harvest (61.30 days) followed by Arka Soumya (61.80 days), which were on par. The highest per centage of pod setting was recorded in HA-4 (14.85) followed by HA-3(14.50), which were on par. Co (Gb) 14 recorded the highest pod length (10.63 cm), pod girth (2.60 cm) and pod weight (6.75 g). Among the twenty five genotypes, nineteen genotypes exhibited light green pod colour, five dark green pod colour and one reddish green pod colour. The highest number of pods plant-1 was found in HA-4 (47.75). The seed colour of eleven genotypes was red, eight pale white, three white, two black and one light brown. The highest number of seeds pod-1 was in Co (Gb) 14 (4.35), which was on par with Arka Jay (4.25) and VRBSEM- 8 (4.25). Co (Gb) 14 recorded the highest weight of 100 seeds (35.45 g). The highest yield of 152.87 was recorded by Co (Gb) 14 which was on par with Arka Jay (148.82g) and HA-4 (148.52g). The highest yield plot-1 of 3.057 kg was recorded by Co (Gb) 14. Two genotypes, viz., Arka Jay (2.976 kg) and HA-4 (2.970 kg) were on par with it. Co (Gb) 14 recorded the highest crude protein and fibre content of 21.40 and 2.050 per cent respectively. The cooking quality of all the twenty five genotypes were found to be good. Incidence of leaf webber and anthracnose disease was noticed. High phenotypic and genotypic coefficients of variation (PCV and GCV) were observed for the characters like days to first flowering, pod setting, pod length, pods plant-1, yield plant-1, yield plot-1 and fibre content. High estimates of heritability coupled with high genetic advance as per cent of mean were recorded for the yield components. Yield plant-1 had a significant positive correlation at genotypic and phenotypic level with raceme length, plant height, pod length, primary branches plant-1, number of racemes plant-1, plant spread, days to first flowering, pod girth and pod weight. Path coefficient analysis revealed that raceme length (0.9760) exerted the highest positive direct effect on yield plant-1 followed by number of racemes plant-1, primary branches plant-1, plant height, pod girth and pod weight. The genotypes were ranked based on selection index score considering the characters viz., plant height, primary branches plant-1, number of racemes plant-1, raceme length, pod girth and pod weight. HA-4 recorded the highest selection index score of 136.72. Based on the mean performance of the genotypes for various characters and selection index score, the top ranking genotypes HA-4, Co (Gb) 14 and HA-3 were found suitable for growing under Kerala conditions.ThesisItem Open Access Evaluation of biostimulants in growbag culture of organic Amaranthus (Amaranthus tricolor L.)(Department of Vegetable Science, College of Agriculture, Vellayani, 2021) Sherinlincy, A; KAU; Refeekher, MThe study entitled “Evaluation of biostimulants in growbag culture of organic Amaranthus (Amaranthus tricolor)’’ was conducted in the Department of Vegetable Science, College of Agriculture, Vellayani in order to evaluate the effect of biostimulants under different application methods in growbag cultivation of organic amaranthus. Amaranthus variety Arun was raised in seed bed and transplanted 21 days after sowing to growbags. Experiments were laid out in CRD with two factors replicated thrice for evaluation of biostimulants and method of application. Foliar application and wick fertigation were the two methods of application under the study for which wick fertigation were installed using pvc pipes and glass wool wicks were used. Biostimulants such as humic acid (3%), humic acid fulvic acid mixture (3%), moringa leaf extract (3%), vermiwash (3%), cow urine (ten times diluted), salicylic acid (50 mgL-1) were compared with RDF (0.22:0.11:0.11 g/plant) and distilled water (control) at weekly intervals. Humic-fulvic acid mixture @ 3% through foliar application significantly increased the height of plant (85.83 cm), leaf length (15.08 cm) and leaf width (6.80 cm). Vermiwash @ 3% through wick fertigation exhibited the highest number of leaves per plant (118.83). Humic – fulvic acid mixture @ 3% through foliar application was also effective in increasing the branches per plant (11.66) while distilled water through wick fertigation recorded least number of branches per plant (4.66). Longest root length (34.16 cm) was recorded when humic acid @ 3% were applied through foliar application compared to control (18.66 cm). Late bolting was recorded with humic acid @ 3%, humic-fulvic acid mixture @ 3% and RDF through foliar application. Earlier bolting was recorded in salicylic acid @ 50 mgL-1 through wick fertigation. Application of salicylic acid @ 50 mgL-1 through wick fertigation significantly increased the leaf/stem ratio (0.50) which was at par with vermiwash @ 3% through wick fertigation (0.45). The total fresh weight of leaves per plant was significantly higher (225.85g) for humic – fulvic acid mixture @ 3% followed by RDF and humic acid @ 3%. At first, second and third harvest, significantly higher yield per cutting was recorded in ten times diluted cowurine (30.64g), RDF (171.62 g), humic – fulvic acid mixture @ 3 % (170.73g) through foliar application respectively while the lowest was recorded by distilled water through wick fertigation. Humic – fulvic acid mixture @ 3% through foliar application also recorded highest yield per plant (646.01 g) compared to distilled water through foliar application (180.72 g). Humic acid @ 3% through foliar application enhanced vitamin C content (98.20 mg100g-1) while ten times diluted cow urine through wick fertigation recorded the lowest vitamin C (57.14 mg100g-1). Oxalate content was observed low for moringa leaf extract @ 3% through wick fertigation with 0.69 mg. Moringa leaf extract @ 3 % through foliar application enhanced the carotenoid content (7.38 mg100-1g). Ten times diluted cow urine through foliar application produced highest calcium content (3.70 mg100-1g). Biostimulants as well as method of application had no significant effect on stem girth and soil parameters such as soil pH, EC, organic carbon, available nitrogen, phosphorus and potassium. In conclusion, biostimulants and foliar method of application was found to be effective for improving growth, yield and quality of amaranthus in growbag organic cultivation. Among the biostimulants, humic – fulvic acid mixture @ 3% produced the highest plant height, leaf length, leaf width, branches per plant, fresh weight of leaves, yield and B:C ratio (1.35) compared to control (0.22).ThesisItem Open Access Breeding hotset indeterminate tomato (Solanumlycopersicum L.) resistant to bacterial wilt suitable for protected cultivation(Department of Vegetable Science, College of Agriculture, Vellanikkara, 2021) Archana, K A; KAU; Pradeep Kumar, TThe present investigation was undertaken to develop an indeterminate hotset tomato with bacterial wilt resistance through line × tester analysis. The experiment was conducted at the Department of Vegetable Science, College of Agriculture, Vellanikkara, Kerala Agricultural University, during the year 2018- 2020. The first experiment was the performance evaluation of 35 tomato genotypes for two seasons in the year 2018, summer evaluation from January 2018 and rainy season evaluation from July 2018, in two protected structures viz., polyhouse and rainshelter. Genotypes exhibited significant variation for vegetative, flowering, fruit, yield and biochemical traits under both growing structures across season. Under polyhouse during summer the genotypes EC- 164263, EC-620387, EC-620401, EC-620406, EC-620410, and EC-620417 performed better for hotset traits (flowers with exerted stigma, pollen viability and fruit set per cent) and yield traits (average fruit weight, number of fruits per plant and yield). In rainshelter, the genotypes EC-165395, EC-165690, EC- 538153, EC-620401, EC-620406, EC-620410 were superior. During rainy season, EC-164263, EC-538153, EC-620401, EC-620406, EC-620410, EC-631369 were superior under polyhouse, and EC-620395, EC-620401, EC-620406, EC-620410, EC-631369 and EC-631379 inside rainshelter, for hotset traits and yield traits. The pooled data of flowers with exerted stigma, pollen viability, fruit set per cent, number of fruits per cluster, days to first fruit set, average fruit weight, pericarp thickness, number of fruits per plant and yield per plant were subjected to multivariate analysis for discrimination of genotypes into clusters. Under polyhouse seven clusters and inside rainshelter five clusters were observed. The selection index was also applied for ranking of genotypes based on the hotset distinctions and yield attributes. The hotset genotypes with high yield potential, identified suitable for protected cultivation were EC-164263, EC-538153, EC- 620387, EC-620389, EC-620401, EC-620406, EC-620410, EC-620417 and EC-631369. The genotypes EC-620401 (1), EC-620406 (2) and EC-620410 (3) were selected as hotset line for the line × tester analysis. In the second experiment the genotypes were tested for bacterial wilt resistance. The 35 tomato genotypes were sown in 98 well pro-trays holding sterilised soil medium. Another set of pro-trays holding the same medium were drenched with bacterial suspension with OD adjusted 0.8 to 1.3 at 600 nm and kept as the sick pro-trays. The twenty-one days old seedlings were used for inoculation through root dip method. With the help of sterilized scissors the tertiary roots of the seedlings were sectioned. The root clipped seedlings were dipped in bacterial suspension for thirty minutes. The inoculated seedlings were transplanted in the sick pro-trays. The entire experiment was conducted inside rainshelter with three replications. Each replication had five plants per genotype. Periodical observation on bacterial wilt symptom expression was taken from third day onwards. The disease index has revealed EC-620382 (0.20) and Arka Abha (0.20) as highly resistant. Nine genotypes viz: EC-165395 (0.24), EC-165700 (0.25), EC-521067 B (0.25), EC-620376 (0.30), EC-620378 (0.28), EC-620427 (0.21), EC-620429 (0.23), Akshay (0.21), Anagha (0.28) were resistant. From these EC-620382 (4), EC-620427 (5), EC-620429 (6) and Arka Abha (7) were selected as testers. In the third experiment, the identified lines and testers were crossed and seeds were collected for twelve cross combinations. The fourth experiment was the evaluation of F 1 hybrids from third experiment during summer in two protected structures viz., polyhouse and rainshelter. The cross combinations were laid along with the parents and two checks viz., one check hybrid (Abhilash) and one check variety (Akshaya). The mean performance, relative heterosis (RH), heterobeltosis (HB), standard heterosis (SH) and combining ability studies were conducted. For vegetative traits, the cross combination 3 × 7 exhibited superiority in the mean performance with significant positive sca effects in both the structures. For flowering traits, the cross 2 × 5 exhibited significant positive sca effects for pollen viability and significant negative sca effects for flowers withexerted stigma with significant RH, HB and SH in both the structures. In the case of fruit and yield traits, the cross combination 2 × 5 exhibited negative significant sca effects for days to first fruit set and positive significant sca effects for pericarp thickness, average fruit weight and yield per plant with significant RH, HB and SH. Inside rainshelter, 3 × 5 and 3 × 7 noted significant sca effects and positive significant heterosis for fruit and yield traits. For biochemical traits, the cross combination 2 × 5 exhibited positive significant sca effects and positive significant RH, HB and SH for TSS, ascorbic acid and acidity under polyhouse. In the seedling stage, all cross combinations exhibited resistance reaction to bacterial wilt in the challenge inoculation. Depending on the mean performance, heterosis and sca effects, the cross combination 2 × 5 was recommended for polyhouse and the crosses 3 × 5 and 3 × 7 were recommended for rainshelter. None of the parents revealed significant and desirable gca effect for all traits studied. The comprehensive assessment of the parents by considering gca estimates revealed that among lines EC-620406 is a good general combiner for vegetative, fruit and yield characters under polyhouse, and EC-620406 and EC- 620410 could be regarded as good general combiners for vegetative, reproductive, fruit and yield, quality characters and shelf life inside rainshelter. In the case of testers EC-620427 and Arka Abha were good combiners for vegetative, reproductive, fruit characters and shelf life in both the growing structures. Dominant gene action was observed predominantly for characters studied under both the structures. Plant height at harvest, days to 50% flowering, days to first fruit set, days to first fruit harvest, number of fruits per plant, average fruit weight, yield per plant, yield per plot, lycopene, ascorbic acid, shelf life, flowers with exerted stigma, pollen viability, number of flowers per cluster, number of fruits per cluster and fruit set per cent exhibited dominant gene action under both the structures. Pericarp thickness exhibited complete dominance inside rainshelter.In the fifth experiment, available SSR markers were validated for bacterial wilt resistance. Two SSR markers, SLM6124 and SLM6-110, produced polymorphism between resistant and susceptible genotypes, two distinct bands for resistant genotypes and four bands (two distinct and two fainted) for susceptible genotypes. In conclusion, the work indicates the change in the behaviour of genotypes under different protected structures in different seasons. Hence the selection of genotype and structure depending on the season is important. The heterosis breeding is effective in improving hotset traits in tomato. The hybridisation involving one resistant parent can generate bacterial wilt resistant off-springs. The application of molecular markers can effectively reduce the time and labour intensive field evaluation.ThesisItem Open Access Evaluation of bush dolichos bean [Lablab purpureus (L.) sweet] for yield and quality(Department of Vegetable Science, College of Agriculture, Vellayani, 2020) Maneesha, P K; KAU; Sarada, SThe present investigation entitled “Evaluation of bush dolichos bean [Lablab purpureus (L.) Sweet] for yield and quality” was conducted at the Department of Vegetable Science, College of Agriculture, Vellayani, from June 2019- December 2020 to evaluate the adaptability of bush dolichos bean in Kerala based on growth, yield and quality. The experimental material consisted of 25 bush dolichos bean genotypes. The experiment was laid out in RBD with two replications. Analysis of variance revealed significant differences among the twenty five genotypes for all the characters studied. Among the genotypes, HA- 4 recorded the highest plant height of 65.75 cm. The variety HA-3 recorded the highest plant spread of 48.95 cm. The highest number of primary branches plant-1 was recorded by HA-4 (5.70), Arka Jay (5.65) and HA-3 (5.55) were on par with it. HA-4 recorded the highest leaf area of 13.35 cm2. Earliest flowering was observed in the genotype VRBSEM- 15 (22.95 days), while first flower appearance was late in HA- 4 (67.00 days). Fifty percent flowering was earliest in VRBSEM- 15 (25.95 days). The highest number of racemes plant-1 was recorded in HA-4 (6.46) and the longest raceme in the variety Arka Jay (34.35 cm). Arka Sambram was the earliest to harvest (61.30 days) followed by Arka Soumya (61.80 days), which were on par. The highest per centage of pod setting was recorded in HA-4 (14.85) followed by HA-3(14.50), which were on par. Co (Gb) 14 recorded the highest pod length (10.63 cm), pod girth (2.60 cm) and pod weight (6.75 g). Among the twenty five genotypes, nineteen genotypes exhibited light green pod colour, five dark green pod colour and one reddish green pod colour. The highest number of pods plant-1 was found in HA-4 (47.75). The seed colour of eleven genotypes was red, eight pale white, three white, two black and one light brown. The highest number of seeds pod-1 was in Co (Gb) 14 (4.35), which was on par with Arka Jay (4.25) and VRBSEM- 8 (4.25). Co (Gb) 14 recorded the highest weight of 100 seeds (35.45 g). The highest yield of 152.87 was recorded by Co (Gb) 14 which was on par with Arka Jay (148.82g) and HA-4 (148.52g). The highest yield plot-1 of 3.057 kg was recorded by Co (Gb) 14. Two genotypes, viz., Arka Jay (2.976 kg) and HA-4 (2.970 kg) were on par with it. Co (Gb) 14 recorded the highest crude protein and fibre content of 21.40 and 2.050 per cent respectively. The cooking quality of all the twenty five genotypes were found to be good. Incidence of leaf webber and anthracnose disease was noticed. High phenotypic and genotypic coefficients of variation (PCV and GCV) were observed for the characters like days to first flowering, pod setting, pod length, pods plant-1, yield plant-1, yield plot-1 and fibre content. High estimates of heritability coupled with high genetic advance as per cent of mean were recorded for the yield components. Yield plant-1 had a significant positive correlation at genotypic and phenotypic level with raceme length, plant height, pod length, primary branches plant-1, number of racemes plant-1, plant spread, days to first flowering, pod girth and pod weight. Path coefficient analysis revealed that raceme length (0.9760) exerted the highest positive direct effect on yield plant-1 followed by number of racemes plant-1, primary branches plant-1, plant height, pod girth and pod weight. The genotypes were ranked based on selection index score considering the characters viz., plant height, primary branches plant-1, number of racemes plant-1, raceme length, pod girth and pod weight. HA-4 recorded the highest selection index score of 136.72. Based on the mean performance of the genotypes for various characters and selection index score, the top ranking genotypes HA-4, Co (Gb) 14 and HA-3 were found suitable for growing under Kerala conditions.ThesisItem Open Access Evaluation of biostimulants in growbag culture of organic Amaranthus (Amaranthus tricolor L.)(Department of Vegetable Science, College of Agriculture, Vellayani, 2020) Sherinlincy, A; KAU; Refeekher, MThe study entitled “Evaluation of biostimulants in growbag culture of organic Amaranthus (Amaranthus tricolor)’’ was conducted in the Department of Vegetable Science, College of Agriculture, Vellayani in order to evaluate the effect of biostimulants under different application methods in growbag cultivation of organic amaranthus. Amaranthus variety Arun was raised in seed bed and transplanted 21 days after sowing to growbags. Experiments were laid out in CRD with two factors replicated thrice for evaluation of biostimulants and method of application. Foliar application and wick fertigation were the two methods of application under the study for which wick fertigation were installed using pvc pipes and glass wool wicks were used. Biostimulants such as humic acid (3%), humic acid fulvic acid mixture (3%), moringa leaf extract (3%), vermiwash (3%), cow urine (ten times diluted), salicylic acid (50 mgL-1) were compared with RDF (0.22:0.11:0.11 g/plant) and distilled water (control) at weekly intervals. Humic-fulvic acid mixture @ 3% through foliar application significantly increased the height of plant (85.83 cm), leaf length (15.08 cm) and leaf width (6.80 cm). Vermiwash @ 3% through wick fertigation exhibited the highest number of leaves per plant (118.83). Humic – fulvic acid mixture @ 3% through foliar application was also effective in increasing the branches per plant (11.66) while distilled water through wick fertigation recorded least number of branches per plant (4.66). Longest root length (34.16 cm) was recorded when humic acid @ 3% were applied through foliar application compared to control (18.66 cm). Late bolting was recorded with humic acid @ 3%, humic-fulvic acid mixture @ 3% and RDF through foliar application. Earlier bolting was recorded in salicylic acid @ 50 mgL-1 through wick fertigation. Application of salicylic acid @ 50 mgL-1 through wick fertigation significantly increased the leaf/stem ratio (0.50) which was at par with vermiwash @ 3% through wick fertigation (0.45). The total fresh weight of leaves per plant was significantly higher (225.85g) for humic – fulvic acid mixture @ 3% followed by RDF and humic acid @ 3%. At first, second and third harvest, significantly higher yield per cutting was recorded in ten times diluted cowurine (30.64g), RDF (171.62 g), humic – fulvic acid mixture @ 3 % (170.73g) through foliar application respectively while the lowest was recorded by distilled water through wick fertigation. Humic – fulvic acid mixture @ 3% through foliar application also recorded highest yield per plant (646.01 g) compared to distilled water through foliar application (180.72 g). Humic acid @ 3% through foliar application enhanced vitamin C content (98.20 mg100g-1) while ten times diluted cow urine through wick fertigation recorded the lowest vitamin C (57.14 mg100g-1). Oxalate content was observed low for moringa leaf extract @ 3% through wick fertigation with 0.69 mg. Moringa leaf extract @ 3 % through foliar application enhanced the carotenoid content (7.38 mg100-1g). Ten times diluted cow urine through foliar application produced highest calcium content (3.70 mg100-1g). Biostimulants as well as method of application had no significant effect on stem girth and soil parameters such as soil pH, EC, organic carbon, available nitrogen, phosphorus and potassium. In conclusion, biostimulants and foliar method of application was found to be effective for improving growth, yield and quality of amaranthus in growbag organic cultivation. Among the biostimulants, humic – fulvic acid mixture @ 3% produced the highest plant height, leaf length, leaf width, branches per plant, fresh weight of leaves, yield and B:C ratio (1.35) compared to control (0.22).
