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ThesisItem Open Access Studies on Capon Production(Department of Poultry Science, College of Veterinary Science, Mannuthy, 1986) Jyotirmoy, Chakraborty; KAU; Sabarinathan Nair, RThe thesis incorporates the results of a feasibility study on economic utilization of Austra-White and White Leghorn surplus cockerels for capon production in the existing Indian conditions. Production performances of surgically caponized Austra-White and White Leghorn males were compared with their respective intacts at three stages, namely, 20th 24th and 28th week of age. A total number of 192 cockerels were involved in the study. Each treatment group comprised 12 birds and replicated four times. Caponization was carried out at 8 weeks of age. The production straits evaluated were body weight gain, feed efficiency, processing losses and yield, percentage ‘slips’, taste panel evaluation, proximate composition of meat and economics of production. Recordings of thermal data, weekly body weight and weekly feed consumption were made. At the 20th 24th and 28th weeks 16 birds randomly selected from each group were subjected to slaughter studies. The results indicated significantly (p<0.05) higher body weight gain for the capons over intact controls at 20 weeks. Austra-White capons were significantly (p<0.05) higher gain at the 24th week but White Leghorn capons did not differ significantly from their intacts at this stage. Capons and intacts did not differ significantly at 28 weeks of age. Feed efficiency was significantly (p<0.05) better for capons during all the periods under tests. The capon registered significantly (p<0.05) higher dressing loss than intacts whereas intacts registered significantly (p<0.05) higher loss due to head and shanks. Ready to cook yield did not differ significantly between treatments. Capons registered increased percentages of moisture, protein and other extract. Five per cent of capons were registered as ‘slips’. Austra-White capon meat top scored in tenderness, juiciness, and personal preference. Economic evaluation registered profitable returns for capons at 20th week, based on culled/spent hen meat value at university farm rates. Significantly higher returns were indicated when economic evaluations were made on market rates. From the results of the study it was concluded that Austra-White surplus cockerels can be effectively utilized for capon production and White Leghorn cockerels form small sized capons. Capons production can be an economic practical feasibility in Indian conditions and also capon meat is preferred over roaster meat. Surgical caponization can be effectively employed as a routine managemental procedure for economic utilization of surplus cockerels. It was also indicated that the optimum economic stage for marketing capons is 20 weeks of age.ThesisItem Open Access Effect of probiotic supplementation on the performance of broiler chicken(Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy, 2001) Sabitha Mahaboob Kadari, A; KAU; Elizabeth, V KThe effects of different levels of pro biotic (Lactobacillus acidophilus, Streptococcus faecium and Yeasacc 1026) supplementation• at 0.025 and 0.05 per cent of the ration on the performance of broiler chicken were evaluated using 144, one-day old, commercial broiler chicks for a period of eight weeks. The birds were divided into three dietary treatment groups viz., standard broiler ration (T 1), standard broiler ration with 0.025 per cent probiotic (T 2) and standard broiler ration with 0.05 per cent probiotic (T3). Standard broiler ration was formulated as per Bureau of Indian Standards (1992) specification for broiler chicken feed. The 0.025 per cent probiotic supplemented birds showed a significantly higher (P<0.05) body weight upto six weeks of age. At the end of eight weeks of age, the 0.05 per cent probiotic fed birds grew faster. The body weight gain was significantly higher in 0.025 per cent probiotic supplemented group upto six weeks of age but was statistically non-significant upto eight weeks of age. The feed intake was not statistically significant throughout the experimental period. Eventhough the feed efficiency was significantly (P<0.01) better in the group fed with 0.025 per cent probiotic at the end of second week, it was statistically non-significant at sixth and eighth weeks of age. The protein efficiency was not significantly different throughout the experimental period. The serum cholesterol levels were significantly (P<0.01) reduced in both the probiotic supplemented groups. The serum protein level was not affected by probiotic supplementation. The processing Yields did not show any significant difference among treatments. The mortality percentage was not affected by treatments. Cost of production of broilers in the 0.025 per cent probiotic group was lower when compared with other two groups at the end of six weeks of age, while it was lower in the 0.05 per cent probiotic supplemented group at the end of eight weeks of age. It can be concluded that probiotic supplementation in standard broiler ration at a lower level was beneficial in the early stages of growth.ThesisItem Open Access Performance of desi x exotic cross-bred layers(Department of Poultry Science, College of Veterinary Science, Mannuthy, 1992) Jayanthy, M V; KAU; Leo, JosephData were collected on egg production performance of two cross-breds viz. desi X Austra White (DAW) and desi X New Rock (DNR) from 20 to 40 weeks of age. The birds in each cross consisted of naked neck and normal neck varieties. DNR cross birds were significantly heavier at 20 and 40 weeks of age than DAW cross birds. The body weight in Naked neck and normal neck varieties comparable in both crosses. Age at sexual maturity was 184 and 189 days in DNR and DAW crosses respectively. Hen housed and hen day egg numbers were similar in both crosses (37.61 and 47.61 vs 34.40 and 47.81), while the naked neck birds in both crosses showed higher hen housed and hen day egg number between the varieties in DAW cross was significant (53.82 Vs 34.86). The eggs were significantly heavier in DNR cross than in DAW cross (46.74 Vs 44.88) whereas the varieties with in both crosses laid eggs of similar size. The mean daily feed consumption and feed efficiency were also similar in both crosses (106.61 g and 4.09 Vs 104.95 g and 4.93). In DAW cross, the naked neck birds registered a better feed efficiency. While DNR birds were multicolored and laid brown eggs, DAW crosses consisted of only black, grayish white with black patches and grey birds which laid tinted eggs. Broodiness was observed in both the crosses. Egg quality was excellent in both the crosses and varieties with in crosses, at 32 weeks of age. Livability was also similar in both crosses though it was poor. Considering the overall performance, the DNR cross was adjudged as a better cross for the backyard.ThesisItem Open Access Dietary protein and energy requirements of caged layers(Department of Poultry Science, College of Veterinary and Animal Science, Mannuthy, 1986) Jalaludeen, A; KAU; Ramakrishnan, AA 4 x 4 factorial trial designed to study the dietary requirements of protein and energy for caged egg type layers under the hot - humid conditions of Kerala is presented in this thesis. Single Comb White Leghorn strain cross pullets (IWN x IWP ) of the Mannuthy centre of AICRP on Poultry for eggs formed the experimental subjects. The birds were randomly alloted to 16 dietary protein-energy combination groups. The dietary protein levels employed were 14,16,18 and 20 per cent and that of energy levels were 2400, 2500, 2600 and 2700 Kcal ME per kg diet. General observations of the experiment were recorded over 12, 28 day periods. The varying levels of protein and energy employed in this study did not have any significant influenced on body weight gain. Birds fed 18 per cent dietary protein had significantly higher hen-day egg production and it was significantly lower for birds fed 14 percent dietary protein. Egg production observed between 16 and 20 per cent and that between 18 and 20 per cent protein were not statistically significant. Hen-day egg production was not influenced by various dietary energy levels employed. The daily feed intake per bird per day was not affect by the le4vels of protein and energy employed in this experiment. A dietary protein levels of 16, 18 and 20 per cent showed significantly superior feed efficiency, when expressed both in terms of feed per dozen egg as well as feed per kilogramme egg. Feed conversion efficiency was not influenced by verying energy levels employed. The egg weight was significantly influenced both by protein and energy levels. However, the changes exerted due to feeding of various dietary combinations did not show any definite trend. The ,mean shell thickness was significantly influenced by dietary protein levels whereas energy did not have any effect. There was a statistically significant decrease in albumen index and Haugh unit score and albumen index was influenced significantly by the energy level in the diet. The yolk index decreased with increase in protein content in the ration, whereas it was inconsistent with the dietary energy levels. Biochemical parameters such as haemoglobin, serum protein, serum inorganic phosphorus and packed cell volume were not influenced either by protein or energy levels. The level of dietary protein did not influence the serum lipid levels whereas the dietary energy had influenced. The dietary- protein-energy combinations in the diet significantly influenced liver lipid and liver lipid and liver protein. The values obtained on carcass analysis were well within the normal biological limits and did not show any specific effect due to different dietary treatments . All the birds showed a positive nitrogen balanced irrespective of the protein or energy levels in the diet. On the basis of these results it can be inferred that the levels of dietary protein and energy required for optimum production for layers raised in cages are 16 per cent and 2400 Kcal ME per kg, respectively. However, in practical feed formulation where amino acids balancing especially that of the critical amino acids being a problems in developing countries it is suggested that a dietary protein level of 18 per cent can be considered as optimal. FINDINGS : A 4 x 4 factorThesisItem Open Access Selection studies in chicken for egg number on part records(Department of Poultry Science, College of Veterinary and Animal Sciencs, Mannuthy, 1986) Maheswar, Rath; KAU; Unni, A K KAll India co-ordinated Research Project on Poultry Breeding for egg located at Kerala Agricultural University, Mannuthy , Trichur is maintaining two strains of white Leghorn which are subjected to selection for high egg production since 1978. The criterion of selection has been IDS method of index selection by combining the information on sire and dam family averages with the individual’s performances, for egg number up to 280 days of age. The present investigation was planned with the following objectives. -To evaluate the response of intrapopulation selection for part period egg production. -To estimate the genotypic and phenotypic parameters for different traits. -To evaluate the the correlated responses in unselected traits. -To measure the time trend in genetic parameters. -To measure the interstrain variation for some economic traits. To achieve these objectives data collected over a period of five generations, including base generation, on the experimental populations were utilized. Chicks hatched during 1979-80 hatching year of each strain constituted the foundation stock (S0). Thereafter both strains were subjected to four generations of selection. Standard managemental throughout experimental period. practices were followed uniformly The traits measured were egg number up to 280 day of age (EP), age at first egg in days (ASM), body weight in grammes at 20 and 40 weeks of age (20 wk-40 wk BW ) and egg weight (BW) in grammes at 30.40 weeks of age. The average effective number of male breeders, female breeders and progenies with complete records were 40, 238.4 and 1165.8 in IWN and 39.8, 232.2 and 1076.6 in IWP strains, respectively, over generations. The effective populations size over generations were 136.62 in IWN and 135.33 in IWP strains. The coefficient of inbreeding increased by two per cent in both populations over four generations of selections which would be the upper limit due to intentional avoidance of sib matings. Since hatch effects were significant for most of the traits, data were corrected for hatch effects before analysis by fitting least square constants. The heritability of each trait and correlations between traits were estimated initially intra generation-strain basis following variance and covariance component anaylsis and then pooled over generations within strain to provide mean estimates. The heritability estimates of various traits obtained in this study were consistent with several earlier reports. Sexlinked effects appeared to be important in the inheritance of EP 20 WK, BN, EW and ASM in IWP strain whereas maternal effects wee important for 40 WK, BN. But sex-linked effects for 40 weeks BN appeared to be important in IWN. The genetic and phenotypic correlations of the present study were close to several earlier reports both in direction and magnitude. The genetic parameter estimates did not show any significant time trends due to four generations of selection in both populations. The realized phenotypic response per generation obtained from regression of generation means on generation number were 8.30 + 1.60 eggs in IWN 5.37 + 1.6-0 eggs in IWP strains, which were significantly different from zero. The correlated response per generation for ASM, 20wk BN, 40 wk BN and BN due to selection for part period egg number were-4.26 d, 28.46 g, - 10.32 g, -0.28 in IWN and –4.45 d, 37.28 g, -13.80 g, -0.02 g in IWP strains, respectively. The realized gains in selected and unselected traits agreed well with the predicated response in direction but not in magnitude, except 40 wk in IWP which was in opposite direction. For all the traits the realized gains were found to be higher than the predicted gains except BN of IWP which was less than the predicated response. Difference were highly significant between the two strains for all the traits under study in the generation except S2 for BN : S0 for ASM, 20 wk BN: S3 for 40 wk BN : S0, S1 and S3 for BN.ThesisItem Open Access Status of broiler production In Kerala with special reference to Trichur(Department of Poultry Science, College of Veterinary, Mannuthy, 1988) Aboobacker, M; KAU; Regunathan Nair, GA survey was undertaken to assess the present status of broiler farming in Thrissur, a major broiler producing district in Kerala. Thirtytwo farms were selected for the survey based on holding strength and frequency of intake. Following are some of the salient points that emerged from the survey. All the farmers were literate and had taken up broiler farming for generating additional income to sustain the family. Majority of the farmers were raising 300 birds per crop with fortnightly intake and were adopting scientific management practices. Organized marketing channel was absent and the birds were disposed off at the farm site itself in retail or wholesale. Dressing of birds in large scale was not being practiced. Results indicated that the mortality rate, body weight at marketing and feed conversion ratio were 6.69 per cent, 1.48 kg and 2.49 respectively. The major portion of production cost was attributed to feed (56.26%) followed by chick (28.56%) and labour (4.31%). Seasons of the year had no appreciable effect on broiler productivity except slight higher mortality due to heat stress during hot seasons. Study also revealed that increasing the stock intake reduces the cost of production with increase in net return. The productivity both in terms of market body weight and livability were adversely affected in farms where hired labour was used resulting in much lower net income. Low profit farms (less than Rs.2.00 per bird sold) showed higher mortality (13.56%) and low body weight at disposal. The survey revealed that better productivity could be achieved by improving efficiency of production particularly reduction in feed cost, increasing livability and adoption of a tangible marketing system so as to obtain remunerative price even during lean seasons.ThesisItem Open Access Effect of dietary supplementation of Baker`s yeast (Saccharomyces cerevisiae) on the performance of broilers(Department of Poultry Science, College of Veterinary and Animal Sciences,Mannuthy, 2004) Renjith, P M; KAU; Amritha ViswanathAn experiment was designed and conducted at the Department of Poultry Science to investigate the effect of feeding baker's yeast on growth, feed consumption and nutrient utilization of broilers. One hundred and forty four day old commercial broiler chicks were reared under three different treatments with four replicates per treatment. The treatment consisted of control diet with standard broiler ration (Tl ) and 1'2 and T3 with 0.3 and 0.6 per cent baker's yeast supplemented respectively with control diet. Chicks were reared under standard managemental conditions up to 8 weeks of age. Broiler starter ration was fed up to 6 weeks of age and finisher ration up to 8 weeks of age. Results of the study revealed that cumulative body weight and body weight gain up to six weeks and eight weeks of age were not affected by the inclusion of yeast. Mean weekly feed consumption recorded significant (P<0.05) difference between treatment during fifth, seventh and eighth weeks of age. Cumulative feed intake up to six weeks and eight weeks of age did not dfffer significantly. But yeast supplemented groups recorded numerically lower feed consumption compared to control group. Feed conversion ratio revealed significant difference (P< 0.05) at sixth week of age recording lower feed efficiency, in 1'2 and T3 compared to control (TJ). But cumulative feed conversion ratio from zero to six weeks and zero to eight weeks of age did not differ significantly. Supplementation of baker's yeast had no effect on processing yields and losses. Numerically lower fat deposition was recorded in yeast supplemented groups than in control group, eventhough there was no significant difference. The addition of yeast at 0.3 per cent and 0.6 per cent levels significantly increased (P<0.05) crude fiber digestibility. Other parameters like retention of dry matter, nitrogen, calcium and phosphorus were not affected by yeast supplementation. Serum protein, serum cholesterol and livability were not affected by yeast supplementation. The net profit per bird was less in yeast supplemented group compared to control group. The higher cost of feed resulted in lower economical return from the treatment groups. The result suggests that inclusion of yeast is not beneficial on economical point of view. However the lower fat deposition consequent to yeast supplementation seems to be advantageous.ThesisItem Open Access Comparative evaluation of membrane protein and biofilm vaccines against duck pasteurellosis(Department Of Poultry Sciences,College of Veterinary and Animal Sciences, Mannuthy, 2008) Indu, K; KAU; Krishnan Nair, CA research work was undertaken to prepare effective vaccines against P. multocida grown under different conditions and assess their immunopotency in one month old ducklings. The purity of the Pasteurella multocida A: 1 strain (DP1) and A: 4 strain (PA4) was confirmed as per standard procedures. Pathogenicity of DP1 and PA4 was assessed in six to eight week old mice. The isolates killed the intraperitoneally inoculated mice within eight hours and within 24 h when injected by subcutaneous route. Pasteurella multocida A: 1 was used for the preparation of different vaccines. The organism was grown in BHIB for preparation of ordinary bacterin. The in vitro biofilm formation of the organism was assessed by growing it under nutrient restricted conditions. For this, the organism was grown in TSB (0.32 per cent) supplemented with 0.3 per cent bentonite clay. For preparation of OMP suspension, the organism was grown in iron restricted condition viz., BHIB supplemented with 100 µM 2, 2’ Dipyridyl and the OMPs were extracted using sodium lauryl sarcosinate. The protein concentration of OMP suspension was estimated to be 3 mg/ml. Median lethal dose (LD50) of DP1 was 10-7.5, which contained 32 viable cells/ ml and that of PA4 was 10-7.38, which contained 24 viable cells/ ml when determined in one month old ducklings. Oil adjuvant vaccines were prepared using ordinary bacterin, bacterin made from biofilm and OMP suspension and performed the sterility, safety and potency tests of the vaccine employing standard procedures. A total of 260 four week old ducklings were divided into four groups with 65 birds in each group and the first three groups were vaccinated with ordinary bacterin, OMP vaccine and biofilm vaccine respectively. The fourth group served as control. The birds were vaccinated with 0.5 millilitre of vaccine intramuscularly in the thigh region. Blood was collected from all the ducks pre-vaccination, at weekly intervals upto 3 weeks post vaccination (PV) and then at 15 days interval upto 60 days, by cardiac puncture or by jugular venipuncture. Passive haemagglutination using GA-SRBC sensitized with sonicated antigen of DP1 was used to measure the humoral immune response. The IHA titres obtained for biofilm vaccine group on day 14 was very much higher than the other two groups. The antibody titre was observed from day seven onwards for all the groups. All the vaccine groups have shown significant difference from the control group at all the stages of the study. On homologous challenging, biofilm vaccine gave higher protection rates of 80 per cent than the 70 and 50 per cent protection rates of ordinary bacterin and OMP vaccine respectively, when challenged with 100 LD50 dose on day 20 PV. On day 60 PV, biofilm vaccine gave higher protection rate of 70 per cent than the 60 and 50 per cent protection rates respectively of ordinary bacterin and OMP vaccine, when challenged with 100 LD50 dose. On heterologous challenging, biofilm vaccine gave higher protection rates of 70 per cent, while only 50 per cent protection was afforded by both bacterin and OMP vaccine, when challenged with 100 LD50 dose on 20 day PV. On day 60 PV, biofilm vaccine gave higher protection rate of 60 per cent while both the other vaccines gave only 50 per cent protection, when challenged with 100 LD50 dose. All the birds challenged on day 40 PV, either with homologous and heterologous organisms died. In most cases, the death occurred due to coliform infection along with stressful factor such as increased atmospheric humidity due to heavy rainfall at that time. In few cases, birds died due to pasteurellosis which might be due to lack of protective level of antibody titre. Biofilm vaccine proved to be the best among the three vaccines tried. Further field trials are to be done before advocating this vaccine for commercial use.ThesisItem Open Access Performance of crosses of indigenous naked neck with white leghorn and new hampshire(Department of Poultry Science, College of Veterinary and Animal Sciences,Mannuthy, 2000) Jomy John, KAU; Leo JosephAn experiment was conducted at Kerala Agricultural University Poultry Farm, Mannuthy, to evaluate and compare the production traits of Naked Neck x White Leghorn (NNWL) and Naked Neck x New Hampshire (NNNH) crosses under deep litter system of rearing from '20 to 40 weeks 'lge. Seventy five pullets of' each cross were housed in identical pens of 15 birds cach and production performance was evaluated for five 28- day periods from February to July 1999. Standard feeding and managemental practices were followed throughout the study . . The NNNH were heavier than NNWL at 20 weeks of age (1855.6 vs l496.lg) and at 40 weeks of age (2436.7 and 2004.1 g). The age at first egg of l46.8±1.48 days in NNNH was significantly earlier than 152±1.3days in NNWL. The age at 50 per cent production was similar in NNWL (162.4 ±1.92 days) and NNNH (164.6 ±1.7 days). The week-wise mean hen housed production in NNWL reached a peak of 80.57 per cent at 26th week of age, while NNNH attained a peak of 81.0 per cent at zs" week of age. The overall mean hen housed number up to 40 weeks of age was 86.39 in NNWL and 83.85 in NNNH. Hen day production remained the same as hen housed production up to 39th week of age and the overall trend was similar. The daily feed consumption from 21 to 40 weeks of age was 124.83g in NNWL and l2S.l4g in NNNH.,The feed conversion ratio per dozen eggs was 2.89 for NNWL and 3.01 for NNNH. Broodiness was shown by nine birds in NNWL for durations ranging from 9 to 63 days while eight birds in NNNH were broody for durations ranging from 22 to 69 days. The mean egg weight from 21 -- 40 weeks was 48.73g in NNWL and 48.44 g in NNNH. The shell thickness was 0.376 mm in NNWL and 0.373 in NNNH. The Haugh unit score was 84.78 in NNWL and 88.00 in NNNH. The plumage Colour in NNWL was off white, but a few birds showed brownish colour on wing feathers. 90 per cent of the NNNH birds had chestnut coloured plumage resembling New Hampshire birds while the rest were multi coloured resembling indigenous Naked Neck birds. Egg shell was tinted in NNWL while NNNH eggs were brown or shades of brown. The livability was 98.7 percent in NNWL, while it was 100 per cent in NNNH. The cost of feed consumed per egg was 202.12 paise for NNWL and 208.94 paise for NNNH [or the entire course of the study. Both NNWL and H cross had statistically comparable hen housed production, egg weight, feed consumption and feed conversion ratio. Both the crosses had excellent livability and good egg quality. However a significantly higher body weight both at 20 and 40 weeks of age, earlier average age at first egg, coloured plumage pattern, and brown shelled eggs in NNNH cross are much preferred by rural farmers, The results of the present study warrants further studies on Naked Neck x New Hampshire cross under field conditions.
