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ThesisItem Open Access Institutional intervention in marketing of non-timber forest products (NTFPs) in Kerala(Department of Rural Marketing Management, College of Co-operation, Banking and Management, Vellanikkara, 2023-05-13) Vijayakumar, N; KAU; Ushadevi, K NIndigenous communities living in the forests depends on the forest resources like Non-Timber Forest Products (NTFPs) for their survival and livelihoods. The dominance of the middlemen, lack of accessibility of the market and lack of awareness of the price of NTFPs were the main problems faced by tribals in the collection and marketing of NTFPs (Rawal, 2020). There is a growing market for NTFPs, but its large potential still needs to be tapped by both the collectors and the institutions involved. Since efforts have been going on for quite a long time, new agencies and institutions, have been established and programmes and schemes have been implemented to promote the NTFPs and thereby develop the tribal community. It is time that an assessment of these interventions be done to assess whether these interventions are within reach of the tribals, to what extent the policy measures of central and state government have positively affect the marketing of NTFPs and the livelihood of tribes collecting NTFPs, whether the institutions address the problems faced by the tribals and to what extent they succeeded in solving the issues related to marketing of NTFPs. In this context the present study entitled ‘Institutional intervention in marketing of Non-Timber Forest Products (NTFPs) in Kerala’ was undertaken to analyse the existing market structure of NTFPs, to examine the role of institutional intervention in promoting NTFPs, to examine the extent of awareness, accessibility and impact of institutional interventions among selected tribes and to analyse the problems faced by the selected tribes in the collection and marketing of NTFPs. This study will fulfil the research gap of the institutional contributions towards improving the livelihood of NTFPs collectors and would help the institutions to identify and prioritise the areas in need of their interventions. Both primary and secondary data were collected for the study. As per the data collected from the records of Scheduled Tribes Service Cooperative Society (STSCS) (2018-2019), it was found that Irular, Kattunayakan, Paniyan, and Kadar were the tribes mainly engaged in the collection of NTFPs. Among them Kattunayakan and Paniyan tribes are settled in Wayanad district, Irular tribe is settled in Palakkad district and Kadar tribe is mainly settled in Thrissur district. Through stratified random sampling method, 204 tribals collecting NTFPs, from these four tribes were selected. With respect to the selection of institutions, all the categories of the institutions involved in promoting NTFPs collectors based in Kerala state were selected with a total sample size of 12 viz Kerala Forest and Wildlife Department (KFD), STSCS, Tribal co-operative Marketing Development Federation of India (TRIFED), Kerala State Federation of SC/ST Development Cooperative Ltd (SC/ST Federation), Scheduled Tribes Development Department (STDD), Kerala State Development Corporation for Scheduled Castes and Scheduled Tribes (KSDC-ST/SC), College of Forestry (CoF), Kerala Institute for Research Training and Development Studies for SC & ST (KIRTADS), Kerala Forest Research Institute (KFRI), Kerala Forest Development Corporation (KFDC) and National Afforestation and Eco-Development Board (NAEDB). Data from tribals were collected through Participatory Rural Appraisal (PRA) method, by using semi-structured interview schedule. Primary data from the institutions were collected from officials of the institutions by using pre-tested structured interview schedule. Secondary data were collected from annual reports, and official websites of selected institutions. Percentage analysis, pair-wise ranking and seasonal calendar were used to analyse the existing market structure of NTFPs. The role of institutional interventions in promoting NTFPs was examined with the help of Venn Diagram. Awareness was examined with the help of percentage analysis. Mobility Map and Impact Diagram were used to examine the accessibility and impact of institutional interventions respectively. Problems of tribals were identified by using Garret Ranking and by drawing ranking ladder. From the analysis of existing market structure of NTFPs in Kerala, it was found that tribals, SC/ST Federation, STSCS, TRIFED, and KFD are the major players in the market. In addition to this the presence of institutions like STDD, KSDC SC/ST, CoF, KIRTADS, KFRI are also found in promoting NTFPs through conducting training, workshops, exhibitions, promoting NTFPs through social media etc. Tribal community are the sellers and STSCS, KFD and TRIFED are the main buyers. A common market structure for NTFPs does not exist in Kerala .An oligopsony market structure was identified where institutions were present and monopolistic competition was observed in the absence of institutions in certain tribal settlements. The STSCS main activity is regularly procuring NTFPs from tribals and marketing NTFPs with the help of the SC/ST Federation through auction cum quotation and negotiation. Eco-Development Committees (EDC) and Vana Samrakshana Samithi (VSS) of KFSD collect NTFPs from tribals and market through Vanasree eco shops, Vanasree e-portal and exhibitions under the brand name Vanasree. Institutions provide a pool of information to help the tribals to undertake their operations more systematically and scientifically. Majority of the tribals prefer to sell the collected NTFPs to STSCS. They had expressed, better price, spot payment, flexible payment options, bulk procurement and regular consumers as the reasons for preferring STSCS over other institutions. Among the institutions TRIFED offers an effective product differentiation support. TRIFED had trained the tribals to produce seven value added products from honey. KFD have an effective supply chain logistics which facilitates the prompt delivery of the product to the customers. . Majority of the tribal community consider KFD, CoF, STDD, and STSCS as important institutions. Out of these they considered STSCS as effective in rendering the service. Institutions had provided product development and market development supports to tribals in additions to the implementation of various schemes of the central and state government. However, the services and supports of the institutions are not benefitting the tribals to the expected level due to their low level of awareness The awareness of the tribals with respect to the interventions of the institutions are very low as they had not received any awareness programme on services offered by the institutions till now. The officials from the majority institutions were not visiting the colony and disseminating the necessary information to the tribals. Most tribals mentioned the forest office, STSCS, and its depot as being conveniently accessible to them. Many institutions that assisted NTFPs collectors (tribals) were far away from the colony, making it difficult for them to access these institutions. The study brought out that institutional interventions had resulted in increasing tribals income, reducing school dropouts, increasing the number of women collectors, reducing tribals debt, and tribals migrations. The major problems faced by the selected tribals with respect to collection of NTFPs were negative interactions with wild animals, inadequate availability of NTFPs in the forest and low price availability of NTFPs. Lack of guidance and support from government officials for marketing of NTFPs, no institutional support/follow up after training programme and long distance to market place cause considerable interruption in marketing of NTFPs. The study brought out the need for certain policy interventions which will help to improve the lively hood of NTFPs collectors. Many marketing channels exist in Kerala for marketing NTFPs and the procurement price and selling price differs based on the channels. If a uniform marketing channel can be created uniform pricing of the NTFPs would be possible.. Even though most of the selected institutions were conducting training programme the intended benefits are not reaching to tribal communities due to the lack of follow up measures. It would be better if institutions arrange follow-up programmes and making monthly visit to tribal settlements. This would help them to sell NTFPs as value added products and build a good relationship with the institutions. As the tribals were unaware of much information, the timely communication of various programmes, services and implementation of schemes etc is necessary. Institutions should take utmost care in this respect. It is difficult for tribals to access the services of institutions, as tribals were situated far away from the institutions and they don’t have vehicle or public transport services. Therefore, it is suggested that institutions should visit the colony monthly to provide their services and identify the problems of the tribals. Inadequate availability of NTFPs in the forest will make a negative impact in their collection and income. Arrangement for clearing the forest every month from the part of the Forest Department will help to address this issue. Addressing these suggestions will help to make the existing interventions of institutions more effective and bring out better reflection on the socio economic upliftment of tribals in Kerala.ThesisItem Open Access Identification of genetic stock for drought tolerance and candidate gene analysis in cocoa(Theobroma cacao L.)(Department of plantation,spices,medicinal and aromatic crops, college of agriculture ,Vellanikkara, 2023-09-21) Suchithra, M.; KAU; Suma, BWater deficit stress is considered as one of the most limiting factor for production in cocoa. Preliminary efforts have been made in Kerala Agricultural University to identify drought tolerant cocoa genotypes. The present study was an attempt made to identify the cocoa genotypes and hybrids that can tolerate drought condition. The study was conducted at Department of Plantation crops and spices, College of Agriculture (CoA) and Cocoa Research Centre and College of Agriculture, Vellanikkara, Thrissur. Thirty exotic genotypes and six pre-released hybrids were taken as materials for the study. They were budded on 6 month old rootstock of seedling raised from polyclonal gardens. GIV 18.5 (progeny of pods from Nileshwar) identified susceptible genotype was used as check in the experiment. Five month old budded plants of thirty exotic cocoa genotypes and six hybrids along with check variety were subjected to drought screening by following gravimetric method. Control plants were maintained at fully irrigated condition under 100 per cent field capacity. Field capacity was maintained at 40 per cent for stress condition. Based on the percentage of retained leaves genotypes and hybrids were categorised as highly tolerant (more than 70% leaves retained), tolerant (40-70% leaves retained), susceptible (10-40% leaves retained) and highly susceptible (less than 10% leaves retained). Various physiological and biochemical parameters related to drought were analyzed. The physiological parameters, relative water content, chlorophyll stability index, membrane stability, photosynthetic rate and chlorophyll content were recorded high in tolerant and highly tolerant genotypes and hybrids whereas these parameters were comparatively low in susceptible and check variety. The control plants at 100 per cent field capacity recorded high value for all these parameters. However in terms of transpiration rate a reverse trend was observed among genotypes and hybrids. Low transpiration rate was recorded in highly tolerant and tolerant hybrids whereas ii susceptible genotypes and hybrids had high transpiration rate under stress condition. The control plants under fully irrigated condition of 100 per cent field capacity also had high transpirational rate. This indicated the ability of tolerant genotypes and hybrids to conserve water during stress. The leaf temperature did not shown any significant variation among the genotypes and hybrids studied under both the field capacity level. In case of biochemical parameters, proline content, glycine betaine content and superoxide dismutase activity were found to be high in tolerant and highly tolerant genotypes and hybrids and was low in susceptible and check variety. The control plants kept under 100 per cent filed capacity level shown less values for all these parameters. However, all the plants showed reduced nitrate reductase activity under drought stress. The control plants recorded high nitrate reductase activity at 100 per cent field capacity level. Correlation studies showed that all physiological and biochemical parameters except transpiration rate and leaf temperature have positive correlation with percentage of leaves retained. Effect of these parameters on percentage of leaves retained was estimated using path analysis. Parameters having direct effect on leaf retention were proline, nitrate reductase activity, SOD, glycine betaine, cell membrane stability and relative water content. These characters were analyzed for their genetic parameters and it was found that all these characters are having high heritability and genetic gain. D 2 statistics analysis revealed that the 30 genotypes and 6 hybrids along with check were grouped into 6 clusters indicating the presence of diversity for different traits. Four different isolation methods were compared in this experiment to identify the best method that could produce high-quality total RNA free from contaminants and genomic DNA. RNA obtained through the SDS- tertiary butanol method (Gesteira et al., 2003) has high quantity and quality, followed by the modified SDSphenol method (Deepa et al., 2014) in both fresh and frozen leaves. iii The transcriptome of highly drought tolerant exotic genotype (T85/799) and highly drought sensitive check variety CCRP 5 was analysed using mRNA sequencing under control (100% FC) and drought stress condition (40% FC). Drought has up-regulated 1540 and 1148 DEGs and downregulated 1502 and 1250 DEGs respectively in tolerant and sensitive libraries. The PCA scatter plot shows that replicates of the well watered samples (control) of highly drought tolerant genotype and drought susceptible check variety CCRP 5 form distinct groups highlighting differences between the two genotypes. Volcano plot depicted the significance of differentially expressed genes in control and drought stress treated samples of highly tolerant and susceptible genotype. In both the tolerant and susceptible genotypes, the majority of the genes within the biological process category were linked to GO terms that fall under the subcategories of "metabolic process," "cellular process," and "single organism process." “Enzyme catalytic activity”, “water channel activity”, “binding” and “transporter activity” were the most abundant terms in molecular function category in both the genotypes under stress. There were 17 pathways significantly enriched (FDR-adjusted p-value < 0.05) in tolerant genotype for upregulated genes and the most enriched pathways including metabolic pathways (178 genes) followed by biosynthesis of secondary metabolites (121 genes), phenyl propanoid biosynthesis (29 genes), carbon metabolism (29 genes), glyoxylate and decarboxylate metabolism (16 genes), MAPK signalling pathway plant (14 genes) and starch and sucrose metabolism etc. Most of the Transcription Factors (TFs) identified families were enriched in, B3, bHLH, WRKY, ARF, ARR B, FAR, LBD, MADS and ERF. These TFs could be grouped into 10 families. The major upregulated genes families in tolerant genotype includes protein kinase, protein phosphatase type 2c, zing finger proteins, laccase gene, cytokinin dehydrogenase, cytochrome p450, galactinol synthase, NAC Domain protein, peroxidase, protein ubiquitin E3 ligase, mannitol dehydrogenase, fatty acid desaturase, UDP glycosyl transferase, Aquaporin PIP, Glutathione dependent genes, iv cellulose synthase and heat shock proteins etc.The major upregulated genes families in susceptible genotype includes LEA protein, PSII protein, peroxisomal membrane proteins, ABC transporter, ABA hydrolyse, phenyl alanine n-monoxygenase, tyrosine carboxylase, plastocyanin, trehalose phosphate phosphatase and expansin etc. RNA-Seq analysis showed that highly drought tolerant exotic genotype (T85/799) activated more number of drought responsive genes than drought sensitive check variety CCRP 5. Several drought-responsive genes were upregulated in the tolerant genotype such as those encoding TFs, cytochrome 450, and membrane transporters, and those associated with carbohydrate metabolism and flavonoid biosynthesis. These genes might confer drought tolerance in this cocoa genotype at the molecular level. Identified drought- responsive genes and metabolic pathways were targets for future studies in order to understand the molecular mechanism of drought tolerance in cocoa. Physiological and biochemical parameters also indicated the better performance of the tolerant genotype over the susceptible genotype under drought stress conditions. These results were a first step to understanding the molecular mechanisms of drought tolerance in cocoa and lay a foundation for its genetic improvement.ThesisItem Open Access Institutionalization of decentralized planning in agriculture in Kerala: trends, determinants and policy imperatives(Department of Agricultural Extension, College of Agriculture,Vellanikkara, 2022-03-30) Abdul Jabbar P K.; Jiju P AlexKerala has successfully evolved a paradigm of decentralized planning by utilizing the provisions of the 73rd and 74th amendments of the constitution. Local governments have been transformed as effective instruments for formulating and implementing development programmes through people’s participation. They are empowered to discharge such functions through strategic devolution of functions, functionaries and funds. Though a robust framework has been evolved over time to make decentralized planning operational, several bottle necks related to local governance affect the efficacy of planning and implementation processes at the grassroots level. This is evident in the productive sector in general and agriculture in particular. It was in this backdrop the present study was conducted. The study was primarily intended to describe the process of institutionalization of decentralized planning in agriculture in Kerala. In this connection, transitions in the administrative framework and policy environment of decentralized planning were also explored. The determinants of the efficacy of decentralized planning in agriculture and the nature of their influence as experienced by major actors of the process were also identified. The study also explored the accomplishments in the agricultural sector since institutionalization of decentralized planning. Alongside, policy imperatives of the experiences of institutionalization were delineated. The sample consisted of 160 respondents comprising of agricultural officers, panchayat presidents and members of the agricultural working group of various development sectors. Respondents were drawn from 40 grama panchayaths selected from five districts representing the five major agroclimatic regions. The study has revealed that there are 15 distinct processes of decentralized planning in the productive sector at the LSGI level. They have evolved over a period of three five-year plans through various iterative processes and drawing lessons from diverse field experiences. The state government plan allocation to local selfgovernments showed decreasing trend from Ninth Five Year Plan onwards. While local self-government institutions (LSGIs) had been provided with 29.29 per cent of state plan fund allocation, it was reduced to 22.9 per cent and 24.2 per cent in the tenth and eleventh five-year plans respectively. Plan expenditure of LSGIs was found to range from 74.81 per cent to 105.25 per cent during this period. It could also be observed that the mandatory sectoral ceilings for productive sector enforced in various plans have succeeded in ensuring investment in various sub sectors of agriculture. Many institutional innovations were initiated to foster people’s participation in planning. Introduction of ward development committees and Ayalsabhas was a major step towards this direction. However, attempts towards additional resource pooling, collaboration with academic institutions to enhance quality of projects and attempts to avoid thin spread of resources were found to be ineffective. Formulating agricultural projects with the assistance of cooperative sector and integrating central and state schemes were also not effective. In spite of the guidelines for formulating joint projects and muti year projects, local bodies were not venturing to such projects, suggesting that a motivational measure to promote joint projects among local bodies be introduced. Moreover, selection of members to the working group and PPC have to be according to their capabilities adjudged based on a state wide criteria. Employing modern technologies for primary processing and value addition, better provision of services, networking of producers and efficient marketing were not adequately mainstreamed by LSGIs in their plans. The efficacy of processes involved in decentralised planning was perceived differently by different categories of actors. Out of the 15 processes, plan appraisal, integration and implementation were perceived to have low efficacy. Plan formulation and resource allocation were also found to have low efficacy. Integration of projects had the lowest efficacy perception score. However, approval of plans by the District Planning Committee, preparation of detailed projects by the working group, holding of Gramasabha, preparation of draft plan proposals and discussion in the development seminar were reported to have high efficacy. Analysis of the attributes of the actors which contributed to their perception on the efficacy of decentralized planning process revealed that four major factors viz. participation, group decision making, experience and knowledge mediation could explain 77.22 per cent variance. Majority of agricultural officers had medium level of perceived efficacy, significantly influenced by their participation-performance interdependence factor and decentralization- development facilitation factors. Role performance of Agricultural Officers in decentralised planning was found to be high. They had performed their roles actively in budgeting of project proposals, planning, monitoring and evaluation, in the order of involvement. Lowest performance level of Agricultural Officers was found to be in functions related to agricultural extension. Accomplishments of decentralized planning in agriculture were quantified in terms physical targets and financial achievements. Plan fund allocation in the productive sector was found to be the highest in the high range zone, followed by problem zone. Plan expenditure in the districts selected from high range zone, northern region and problem zone was higher than 80 per cent. Projects on various aspects of rice, vegetables and banana had received more funds than other sub sectors during the reference period. With regard to the allocations spent, assistance to labour cost for rice farmers was the major intervention while those for coconut development and promotion of intercropping were less than 10% and 3% of respectively. Compared to other crops, the attention paid by local bodies to development of tuber crops, spices and pulses was less. Cashew being a hardy crop for waste lands did not find any place in decentralized plans of north, central and high range zones. Several deficiencies were found in the process of decentralised planning. Most importantly, there should be focused efforts for enhancing the capacities of working groups and Planning Committees. Integration of development interventions by various agencies at the local level have to be ensured through better coordination. Collective deliberations on sectoral working group reports coordinated by panchayath planning committee will foster shared vision and convergence among functions of various officials transferred to the Grama panchayath. The panchayath level data base, Peoples’ Bio Diversity Register formulated through Bio Diversity Management Committees and the watershed master plan have to be updated through massive farmer participation to ensure data base support to local planning and foster the opportunities for local resource mobilization. Further, assistance for conservation and multiplication of local germplasm, traditional seed reserves by local farmer clusters, community seed banks and seed growers’ networks have to be initiated and the subsidy guidelines have to be broadened to include such initiatives. The perception of poor marketing infrastructure was the most ranked among various constraints analyzed. With regard to projects to bridge the skill gaps in farming and improved service delivery, the existing Agro Service Centres (ASC) at block level and Farmer Service Centres should be networked for technologybased services with supporting projects from decentralized plan allocations. A KVKATMA- LSGD linkage will resolve the issues of less propagation of technologies in projects and less expertise of working group members. To enhance participation of youth in gramasabha a drive on decentralization, gramasabha literacy and the need to participate for youth was suggested as part of school and college syllabus.ThesisItem Open Access Endophytic fungi for the management of spotted pod borer, Maruca vitrata Fab.(Lepidoptera:Crambidae) in cowpea.(Department of Agricultural Entomology, College of Agriculture ,Vellanikkara, 2022-12-09) Smitha Revi; Madhu SubramanianThe spotted pod borer, Maruca vitrata Fab. (Lepidoptera: Crambidae) is a major constraint in the production of legumes because of its wide host range, distribution and destructiveness. In cowpea, the loss due to pod borer infestation varies from 20 to 60 per cent, often reaching upto 80 per cent in severe cases. Application of pesticides can hardly be recommended in cowpea as the pods are harvested at alternate days. Biological control of M. vitrata using entomopathogenic microorganisms has received considerable attention in recent times. Previous studies have demonstrated the potential of entomopathogenic organisms to colonise an array of plants and confer protection from insect pests. In this context, a study entitled “Endophytic fungi for the management of spotted pod borer, Maruca vitrata Fab. (Lepidoptera: Crambidae) in cowpea” was undertaken with an objective of isolation and characterization of endophytic entomopathogenic fungi in cowpea, standardization of method of inoculation of entomopathogenic fungi in cowpea, and evaluation of selected endophytic fungi for management of the spotted pod borer, M. vitrata. Purposive sampling surveys were conducted in the major cowpea growing areas of Kozhikode, Thrissur, Kottayam and Thiruvananthapuram districts of Kerala. A total of 235 endophytic fungal isolates were obtained from the cowpea plant samples collected from 40 locations. This comprised of 103 isolates from roots, 63 from stems, 31 from leaves, 33 from pods and five from flowers. Nine accessions of cowpea with different levels of resistance were also screened for the isolation of fungal endophytes. All the accessions were found to harbor endophytic fungi and yielded 32 isolates. Among these accessions, Palakkadan thandan payar yielded the maximum number of seven endophytic fungi. Lola and Mysore local had the lowest number of isolates. In contrast to the results of survey, the leaves of cowpea plant harbored more endophytic fungi than other plant parts. A total of 267 isolates were obtained from survey samples and cowpea accessions. Three isolates were found to be pathogenic to M. vitrata. They were identified as Fusarium oxysporum (EEF 1) and two isolates of Purpureocillium lilacinum (EEF 4 and EEF 64) through morphological and molecular characterization. These isolates were evaluated along with Beauveria bassiana (NBAIR strain) for their bioefficacy against M. vitrata. The best two organisms in the bioefficacy studies, viz., B. bassiana (NBAIR strain) and P. lilacinum (EEF 4) were used for standardizing the inoculation technique for endophytic colonization in cowpea plant. Three different methods of inoculation viz., seed, soil and foliar inoculation were evaluated for identifying the best method for colonization of entomopathogenic fungi in cowpea plants. The effect of fungal colonization on infestation of spotted pod borer in cowpea was further assessed under field condition. Foliar inoculation with B. bassiana registered the lowest mean pod damage of 12.53 per cent. B. bassiana applied as foliar application resulted in the highest marketable pod yield of 152.83g plant-1 , followed by P. lilacinum as foliar application (149.33g plant-1 ). Based on the polyhouse and field studies, it was inferred that foliar application of B. bassiana was found to be the best treatment against the target pest, M. vitrata. In addition, the results of the current study suggested that that endophytic colonization in cowpea had little adverse impact on plant growth and yield. Foliar application of B. bassiana, which was identified as the most effective treatment against pod borer in the previous studies, was selected for comparative evaluation with the diamide insecticide, flubendiamide against M. vitrata. Cowpea plants treated with both flubendiamide and B. bassiana had significantly less number of infested pods than control, with 8.41 and 15.05 per cent infestation respectively. Control plants recorded the highest infestation of 21.28 per cent. Mean marketable pod yield showed significant difference between flubendiamide (166.14g plant-1 ) and foliar inoculation of B. bassiana (155.14g plant-1 ). Both these treatments also had significantly higher marketable pod yield compared to control plants (139.29g plant-1 ). In conclusion, the present study revealed that use of B. bassiana as an endophyte could be a useful tool in integrated pest management of pod borer in cowpea. However, further studies are needed to understand the mechanisms through which the endophyteThesisItem Open Access Spatial mapping of flood prone areas and risk assessment of Chalakudy river basin using HEC-HMS and HEC-RAS models(Department of Irrigation and Drainage Engineering, Kelappaji college of Agricultural Engineering and Technology,Tavanur, 2021-10-17) Gudidha Gopi.; Rema, K P.Floods are one among the most devastating natural disasters that affects life on the globe. For the planning and design of water resources projects in the preferred area, planners and engineers usually require reliable estimates of flood magnitude and frequency. Kerala state in the Indian sub continent received a catastrophic flood in the year 2018. The present study attempts to model the flood flows and map the flood prone areas of a river basin in Kerala. The Chalakudy river basin, one of the worst-affected river basins due to heavy rains and floods was selected for the present study. This is the fifth largest river in Kerala. The basin is predominant with agricultural land and falls under the humid tropical zone, where water resources planning and management is necessary for irrigation scheduling, flood control and design of various engineering structures. In order to address the above issues, an attempt was made to calibrate and validate HEC-HMS model for simulating the flood hydrograph for the Chalakudy river basin. Flood frequency analysis was carried out to estimate the flood peak values using frequency distributions in HEC-SSP software. The results were compared with the estimated flood peak values for different return periods obtained from the HEC-HMS model. Hydraulic routing was done in HEC-RAS model and the flood inundation maps were prepared. The cadastral level risk areas were identified based on water surface profiles of velocity and depth of flood extent and its characteristics. Food vulnerability maps based on land use patterns were developed in order to identify the severely affected land uses. The HEC-HMS model for the basin was developed using SCS-UH, SCSCN, Recession and Muskingum methods to find out the loss rate, runoff transformation and routing of flood respectively. Statistical performance indices of the model, Nash-Sutcliffe efficiency (NSE) and Coefficient of correlation (R²) values were obtained above 0.7, Error in Peak Flow (%) and Error in Volume (%) were figured below 20% and Root Mean Square Error-Standard Deviation Ratio (RSR) was acquired as 0.5 and below. These values indicated that HEC-HMS model simulation performed well in both calibration and validation. The frequency discharge values calculated using Log Pearson type-III distribution indicated a high degree of similarity to the HEC-HMS generated values with an R 2 value of 0.862. The results of the Log Normal and Gumbel distributions are significantly lower than those of the HEC-HMS model values. The assessment of the vulnerability due to the flooding was made with regard to the land use pattern and cadastral level risk map of Chalakudy river basin was developed for different return periods. Kadukutty Panchayat located in the downstream of Chalakudy river basin was found to be the maximum flood inundated area for 10 year return period ( 557 ha) and for 200 year return period (681 ha). Manjapra Panchayat located in upstream was found to be the least flood inundated area for 10 year return period (6 ha) and for 200 year return period (9 ha). Annamanada, Kadukutty, Melur and Pariyaram panchayats were under high risk areas, with depths greater than 20 m. Ayyampuzha, Chalakudy, Mala, Kuzhur, Parakkadavu and Puthenvelikara panchayats were under medium risk areas with depths varying from 10 to 20 m. Athirappilly, Manjapra and Karukutty panchayats were under low risk areas with depths less than 10 m. The flood vulnerability maps were generated by intersecting the flood plain land use map with the flooded area polygons. Paddy land near to the river banks was found to be the highest inundated by different return period floods, followed by forest and other vegetation, barren land and other land use classesThesisItem Open Access Breeding yard long bean(Vigna unguiculata subsp.sesquipedalis (L.) Verdcourt) for anthracnose resistance through conventional and molecular marker analysis(Department of Vegetable Science College of Agriculture ,Vellayani, 2022) Merin Elza George; Sarada,SThe study entitled ―Breeding yard long bean (Vigna unguiculata subsp. sesquipedalis (L.) Verdcourt) for anthracnose resistance through conventional and molecular marker analysis‖ was carried out at the Department of Vegetable Science, College of Agriculture, Vellayani, Thiruvananthapuram, Kerala, during 2019-2022. The objective of the study was to identify molecular marker(s) linked with anthracnose disease resistance in the F2 segregating population of yard long bean, using bulk segregant analysis to enable marker assisted selection along with conventional breeding. The study was undertaken in six experiments. In experiment I, 50 genotypes of vegetable cowpea collected from different parts of India, including the released varieties of SAUs and ICAR institutes were screened against the most virulent isolate of Colletotricum gloeosporioides through artificial inoculation. Among the 50 genotypes tested, VU 53, with semi-trailing growth habit was found to be highly resistant with disease severity of 3.67 ± 0.13 %. Three susceptible, high yielding long podded varieties viz., KAU Deepika, Vellayani Jyothika and Githika, selected as female parents were crossed with VU 53, the most resistant genotype as male parent for the development of three hybrids. In experiment II, two crossing blocks were laid to produce the seeds of three hybrids and their F2 progenies. The three hybrids were evaluated along with the parents under replicated trial in the main field for trailing nature, yield and quality in experiment III. Significant variation was noticed on mean performance of yard long bean parents and hybrids for most of the characters studied. Earliest flowering was observed in KAU Deepika x VU 53 (57.33 ± 0.57 days) and delayed flowering in Githika x VU 53 (71.66 ± 1.15 days). Among the hybrids, the highest pod length was recorded in KAU Deepika x VU 53 (24.50 ± 1.00 cm), while the Vellayani Jyothika x VU 53 had the maximum pod girth (2.90 ± 0.10 cm). Maximum pod weight and pods per plant was recorded in KAU Deepika x VU 53 (19.22 ± 0.58 g and 79.33 ± 0.59 respectively) among hybrids. The cross Vellayani Jyothika x VU 53 (16.55 ± 0.20 g) exhibited maximum seed weight. Among the three hybrids, KAU Deepika x VU 53 (1416.00 ± 126.09 g) recorded the highest yield per plant and was on par with Githika x VU 53 (1343.33 ± 75.06 g). Among the hybrids, KAU Deepika x VU 53 (67.85 ± 0.96 days) was significantly superior for days to harvest. The longest duration among hybrids was observed in KAU Deepika x VU 53 (126.56 ± 0.37 days) and the shortest duration was recorded in Vellayani Jyothika x VU 53 (121.31 ± 0.58 days). High heritability and genetic advance was recorded for vine length, days to first flowering, pod length, pod weight, pods per plant, yield per plant, yield per plot, hundred seed weight and keeping quality. Yield per plant had significant positive phenotypic and genotypic correlation with pod weight, followed by pods per plant, length of terminal leaf, seeds per pod, pod length, crop duration, keeping quality and days to harvest. Pods per plant could be considered as the most important yield trait for enhancing the yield in yard long bean because of its high, positive correlation and direct effect with yield per plant along with high heritability and genetic advance. In experiment IV, parents, three hybrids and F2 progenies were evaluated for resistance to natural incidence of C. gloeosporioides in the main field, as unreplicated trial and without any plant protection measures. All the three F1 were found to be field tolerant. Out of 175 F2 plants of the cross KAU Deepika x VU 53, 164 plants were susceptible and 11 were resistant. In the case of Vellayani Jyothika x VU 53, 175 plants were susceptible among the 187 individual F2 progenies observed. Similarly, in the cross Githika x VU 53, 188 plants were susceptible while 12 plants were found to be symptomless. The F2 progenies of the high yielding hybrid KAU Deepika x VU 53 were evaluated for resistance to C. gloeosporioides through artificial inoculation in experiment V. Out of 196 plants, 185 plants were found to be susceptible whereas 11 plants were resistant which could be utilized in marker assisted breeding programme. The segregation for anthracnose resistance in F2 plants was in the ratio 15:1 (185 susceptible plants out of 196), which suggests that the inheritance of anthracnose resistance may be controlled by two recessive genes. Bulked segregant analysis was done using the DNA from the resistant parent, susceptible parent, resistant F2 bulk and susceptible F2 bulk in experiment VI. Bulked segregant analysis with the SSR primer CLM0008 revealed a polymorphic band at ≈ 300 bp which was appeared in the resistant parent and bulk. Bulked segregant analysis with ISSR primer UBC 811 produced polymorphic bands at ≈ 1100 bp, which were present in resistant parent and resistant bulks. The identified markers linked with resistance were validated over the resistant F2 lines obtained.ThesisItem Open Access Response of banana Musa (AAB) 'Nendran' to nutrient sources(Department of Fruit Science, College of Agriculture ,Vellanikkara, 2021) Manohar Lal Meghwal.; Jyothi, M L; KAUanana is the leading tropical fruit in the world market today with a highly organized and developed industry. Banana having a root system spread in the top 60 cm soil, is heavy feeder of nutrients and requires large quantities of nutrients for its growth, development and yield. Nutrient removal from soil by crops must be replenished. Under good management conditions and adequate supply of biofertilizers and organic manures, the nutrient removal can be replenished and soil physical, chemical and biological properties can be improved. Organic and inorganic sources of nutrients have significant influence on fruit quality and soil characteristics. The current agricultural policy emphasize a shift towards safe agricultural practices for which organic management is the best option. However the crop behaviour under organic and inorganic management needs elaborate studies. Hence the research entitled ‘Response of banana Musa (AAB) 'Nendran' to nutrient sources was formulated to elucidate response of banana in terms of growth, yield and quality to nutrient sources and to compare the fruit quality of banana grown under organic and conventional systems in farmer’s field. The study revealed that vegetative growth of Musa Nendran banana was not influenced by different sources of nutrients in early stage in both the years but later differences were recorded between the treatments. Plant height, number of leaves and pseudostem girth showed significant differences from 90 DAP. At bunching stage plant height and pseudostem girth were higher in organic treatments. There was significant difference in pseudostem girth between the treatments throughout the growth stage of Nendran banana. Among the treatments, T8 resulted in better growth of plants. Leaf characters like number of leaves and leaf area index were not influenced significantly between organic and integrated nutrient management. Early leaf production was also noticed in treatment T8 as indicated by the observations on phyllocron. Growth was delayed in control where no manures and fertilizers. In general more number of leaves per plant and lesser duration for leaf emergence was recorded in both the years in organic treatments. Chlorophyll production in the index leaf of banana was influenced with organic and inorganic nutrition. Chlorophyll a, b and total chlorophyll in the index leaf were distinctly higher in treatment T8 (fertigation with FYM) which was on par with treatments T3 and T5 where organic manures alone were applied. Early flowering and early harvesting were observed in organic treatments. Higher total biomass production was recorded in organic treatments. Yield and yield attributing characters like bunch weight, number of finger, finger weight were highest in treatments with organic sources of nutrients. The mean bunch weight was influenced significantly by organic and inorganic sources of nutrients. Fertigation with organic sources of nutrients resulted in the production of heavier bunches in both years. Maximum bunch weight was recorded from treatment T8 which was on par with other organic treatments as well as integrated management with fertilisers applied as fertigation as well as based on soil test results. No significant variation was observed between treatments on number of hands per bunch and finger characters like finger length and girth. Peel thickness of fruits were not significantly influenced but the pulp to peel ratio was significantly influenced by the treatments. Pulp to peel ratio was higher in all treatments other than T1 and control where T1 is the POP recommendation for TC banana under integrated nutrient management. Yield per plant was positively correlated with available N, P, K, Calcium, magnesium, sulphur, Zn, Cu, B, content of the soil. Yield was also positively correlated with soil properties like pH, organic carbon content, CEC, Bulk density, and Dehydrogenase enzyme activity. Higher biomass production was recorded in plants that received nutrients from organic sources compared to integrated nutrient management and control. Shelf life of fruits were improved in organic treatments. Fruit quality parameters like TSS, Total sugars, ascorbic acid and β carotene of ripe banana fruits were improved in organic treatments compare to inorganic system. Sensory score of ripe fruits and fruit chips were maximum in organic treatments. The taste of ripe banana fruits was improved in plants grown under organic treatments. Fertigation with organic manures (T8) resulted in improved fruit quality of Nendran banana in both the years. Different soil physical and chemical properties also improved when nutrients were supplied through organic sources. Soil pH, electrical conductivity, organic carbon content, cation exchange capacity, available, N, P, K, Mg, Iron, Copper, Zinc, Mn and boron were better in soils receiving organic manures alone. Similarly the soil biological properties like dehydrogenase activity, nitrogenase activity, microbial biomass carbon, and viable counts of total fungi, bacteria and actinomycetes were better in organic treatments. Bulk density of soil was low in soils receiving organic manures alone compared to integrated nutrients. Total uptake of nutrients in organic and integrated nutrient management system was compared. Uptake of N, Ca, S, Fe, Mn, Zn and Cu was higher in organic system of cultivation of banana compared to integrated system. Higher benefit cost ratio was recorded banana grown in organic system. The study revealed that organic sources of nutrients improved soil properties and thereby improved growth, yield and quality of banana.ThesisItem Open Access Integrated nutrient management in minisett cultivation of elephant foot yam {Amorphophallus paeoniifolius (Dennst.) Nicolson}(Department of Agronomy, College of Agriculture, 2022) Dhanalakshmi V N.; Rajasree GThe study entitled “Integrated nutrient management in minisett cultivation of elephant foot yam [Amorphophallus paeoniifolius (Dennst.) Nicolson]” was conducted at the Instructional Farm, College of Agriculture, Vellayani, Thiruvananthapuram to standardise the minisett size in elephant foot yam and to investigate the effect of integrated nutrient management practices on growth, yield, quality and economics of cultivation and to study the rooting and tuberisation pattern of minisett planted elephant foot yam. The investigation consisted of two experiments; standardisation of minisett corm size and integrated nutrient management practices (field experiment) and rooting and tuberisation pattern study (pot culture) and was undertaken during April to November 2018 and 2019. The first experiment was laid out in RBD with 15 treatment combinations and a control, replicated thrice. The treatments comprised three minisett corm sizes (s1-200 g, s2-300 g and s3-400 g) and five integrated nutrient management practices (i1-100 per cent NPK, i2-75 per cent NPK with 50 per cent N substitution through coir pith compost, i3-75 per cent NPK with 50 per cent N substitution through coir pith compost + PGPR mix-I + AMF, i4-50 per cent NPK with 50 per cent N substitution through coir pith compost, i5-50 per cent NPK with 50 per cent N substitution through coir pith compost + PGPR mix-I + AMF). The minisett corms and control corms (1 kg) of elephant foot yam var. Gajendra were planted at a spacing of 60 x 60 cm and 90 x 90 cm respectively. The recommended dose of N, P and K (100:50:150 kg NPK ha-1 ) for elephant foot yam was modified based on soil test data. Substitution of N with coir pith compost was carried out on N equivalent basis, and P and K were given through chemical sources. The pot culture experiment was laid out in CRD with 14 plants per treatment with the same treatments as the field experiment. The number of days taken for 100 per cent sprouting of seed corms varied between minisett corms and control corms and the latter sprouted early. The minisett corm s3 (400 g) recorded taller plants, higher leaf area index, pseudostem 281 girth and canopy spread. Application of 100 per cent NPK (i1) as chemical fertilizer produced taller plants and higher canopy spread during later stages compared to INM practices. Among the INM treatments, i3 (75 % NPK with 50 % N substitution through coir pith compost + PGPR mix-I + AMF) found superior with respect to growth attributes such as plant height and canopy spread. The treatment combination s3i1 (400 g + 100 % NPK as chemical fertilizer) produced taller plants and recorded higher canopy spread. Among the interactions including INM practices, taller plants were produced by s3i3 and s3i5 and higher canopy spread was recorded with s3i2, s3i3 and s3i5. Control plants (1 kg) showed taller plants, higher pseudostem girth and canopy spread at all stages of observation. The s3 recorded higher yield attributes, corm yield (48.81 and 50.57 t ha-1 during first year and second year, respectively) and pooled corm yield (49.69 t ha1 ). The treatments, i1 (100 % NPK) recorded significantly the highest yield attributes and yield. Among the different INM practices, i3 recorded higher yield attributes and corm yield (38.26 and 45.37 t ha-1 during first year and second year, respectively), while corm yield in pooled mean analysis (41.82 t ha-1 ) was also higher in this treatment. The s3i1 (400 g + 100 % NPK as chemical fertilizer) produced significantly the highest yield attributes and yield among all the treatments. Among the INM combinations, s3i3 recorded higher yield attributes, corm yield and higher pooled mean of corm yield (51.29 t ha-1 ). Control recorded higher yield attributes than minisetts. Pooled analysis of corm yield ha-1 indicated that s3i1, s3i2, s3i3 and s3i5 were superior to control and s2i1, s2i2, s2i3 and s3i4 were on par with control. Quality attributes like starch, total sugar, crude protein and crude fibre content of corm were non significant with respect to the treatments. Higher dry matter content was obtained in i4 (50 % NPK with 50 % N substitution through coir pith compost). The lower content of oxalic acid was recorded with i5 and i4 and the higher content was in i1 (100 % NPK). The minisett corm s3 recorded superior results for nutrient content, uptake and nutrient harvest indices. The INM treatment, i3 recorded higher nutrient content and uptake, however the highest was recorded in i1 among all the treatments. The treatment s3i1 (400 g + 100 % NPK 282 through chemical fertilisers) recorded significantly the highest K uptake. Among the combinations including INM practices, s3i3 recorded higher K uptake during second year. The combinations, s3i1, s3i2, s3i3, s3i4 and s3i5 were found superior to control in case of nutrient uptake. Soil chemical properties after the field experiments were not significantly affected by the treatments. The treatment s3i1 (400 g + 100 % NPK) recorded the highest net income and BCR during both the years. The highest net income and BCR were recorded from s3i2 during first year and s3i3 during second year, and the two year mean of net income and BCR were also highest in the case of INM treatment s3i3. In pot culture study, chemical properties of potting medium at monthly intervals up to harvest were not significantly affected by the treatments except for organic carbon content. The treatment i3 (75 % NPK with 50 % N substitution through coir pith compost + PGPR mix-I + AMF) recorded higher organic carbon content at 5 MAP. The minisett corm s3 excelled in rooting pattern and root anatomical parameters. Higher number of roots per plant was recorded in i2 and i3, however, these treatments were on par with i1 (100 % NPK). Higher weight of roots per plant was observed in i5, i4 and i3 and, root anatomical parameters were superior in i3. The interactions, s3i5, s3i4 and s3i3 recorded the highest weight of roots per plant. Higher root parameters were recorded in control than in minisetts and, in case of number of roots per plant s3i1, s3i2 and s3i3 at 4, 6 MAP and harvest, and s3i1 at 5 MAP recorded on par results with control. In the case of weight of roots per plant, s3i3 was on par with control at harvest. The roots of plants applied with AMF showed mycelial network from 3 MAP up to the harvest, and in the maximum growing stage of 5 MAP, vesicles were found in between the cells of the roots of AMF applied plants. Higher root colonization was observed in s3i3 at 5 MAP. Corm initiation was observed between 1 MAP and 2 MAP in control and between 2 MAP and 3 MAP in all other treatments. The s2 (300 g) during 3-4 MAP and s3 (400 g) during all other stages had significantly the highest corm bulking rate (CBR). Higher corm bulking efficiency (CBE) was recorded in s2 during 3-4 MAP, s1 during 4-5 MAP and 6 MAP-harvest and s3 during 5-6 MAP. 283 The INM treatment i3 showed superior results for CBR and CBE, however, higher CBR and CBE were recorded in i1 (100 % NPK) among all the treatments. The s3i1 (400 g + 100 % NPK)showed the highest CBR and CBE during 4-5 MAP and 5-6 MAP among all the treatments. Among the INM interactions, s3i3 during 3-4 MAP and 5-6 MAP; s3i2 during 4-5 MAP and s3i4 during 6 MAP-harvest recorded higher CBR. The s2i3 during 3-4 MAP, s1i3 during 4-5 MAP, s3i3 during 5-6 MAP and s1i5 during 6 MAP-harvest recorded higher CBE. Higher corm weight per plant was recorded with s3. The i1 recorded the higher corm weight per plant among all the treatments, and at 5 MAP, i1 was on par with i3. Among the INM treatments i2 at 3 MAP and i3 at all other stages recorded higher corm weight per plant. Among interactions, s3i3 produced higher corm weight, however, among all the treatments the highest corm weight was noted in s3i1 at all stages except at 4 MAP. Control produced significantly higher corm weight per plant than minisetts. Uptake of nitrogen at 3 MAP and uptake of phosphorus at harvest were higher in s3. The INM treatment i3 recorded higher microbial population and dehydrogenase activity in the potting medium and among the interactions, higher dehydrogenase activity was recorded with s3i3. Significant and positive correlations were observed between corm weight per plant vs. root anatomical parameters and nutrient uptake vs. root anatomical parameters. It is evident from the present study that planting of 400 g minisett corm resulted in better growth, yield and quality of elephant foot yam. Application of 75 per cent NPK with 50 per cent N substitution through coir pith compost + PGPR mix-I [@ 10 g per pit (dry cow dung: PGPR mix-I in 50:1 proportion) - at planting and 2 MAP] + AMF (@ 10 g per pit - at the time of planting) in elephant foot yam resulted in superior growth, yield and quality under INM system. Planting of 400 g minisett and application of 75 per cent NPK with 50 per cent N substitution through coir pith compost + PGPR mix-I + AMF under an INM system could be recommended for economic production of minisett elephant foot yam. Rooting and tuberisation of elephant foot yam were found superior in planting of 400 g minisett corm with application of 75 per cent NPK with 50 per cent N substitution through coir pith compost + PGPR mix-I + AMF.ThesisItem Embargo Genetic diversity analysis for nutrient efficiency and identification of nutrient responsive genes in cassava (Manihot esculenta Crantz)(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2023-02-10) Swathy Sivan; KAU; Arya, KThe present study entitled “Genetic diversity analysis for nutrient efficiency and identification of nutrient responsive genes in cassava (Manihot esculenta Crantz)” was carried out in the Department of Plant breeding and Genetics, College of Agriculture, Vellayani and Division of Crop Improvement, ICAR-CTCRI, Sreekariyam, during 2019-2022. The study was undertaken to screen and characterize N and K-efficient genotypes in cassava and identify the traits contributing to nutrient efficiency and study their gene expression. Thirty genotypes of cassava comprising of landraces, released varieties and pre-release accessions from ICAR-CTCRI and KAU were taken for the study. Field evaluation was done to identify low input nutrient efficient lines where analysis of variance revealed significant difference among the genotypes for all the characters studied except the girth of tuber. High GCV, PCV, heritability and genetic advance were observed for traits like tuber yield, N and K efficiency, plant height, number of leaves, number of branches, stem N and K content. Tuber yield was positively correlated to N and K efficiency, plant height, leaf and stem weight, stem girth, mean weight of tubers, number of nodes, girth and length of tubers, while negatively correlated to tuber starch and dry matter content. Path analysis showed that tuber length gave highest positive direct effect on yield followed by number of tubers, number of leaves, tuber starch content, plant height and nitrogen efficiency. Diversity studies grouped the genotypes into eleven clusters and the characters that gave the maximum contribution to divergence were tuber dry matter content, N and K efficiency. Root studies on thirty genotypes revealed that root traits like number of nodal roots, basal roots, adventitious roots, storage roots and root length displayed significant positive correlations with N and K efficiency. Evaluation of five highly nutrient efficient genotypes at the three levels of fertilizers (0%, 25% and 50% of the recommended dose of fertilizers) showed overall significance for traits like plant height, the number of leaves retained, stem weight per plant, girth of the stem, tuber yield, length of tuber, stem K content, tuber K content, total N and K uptake, N and K efficiency. The highest yielder was KBH 18 followed by 8S501-2. Maximum N efficiency was observed for KBH18 followed by 15S409, while maximum K efficiency was recorded for 8S501-2 followed by 15S409. Results showed that from 0 to 25 % there is an escalation in tuber yield as well as N and K efficiency, while the values at 25 and 50% are on par. All the selected four genotypes showed significant superiority over the K efficient check variety – Sree Pavithra – in terms of tuber yield, N efficiency and K efficiency. The study identified nine nutrient responsive genes in cassava which includes NRT1, NRT3, NLP1, GPT2, AMT1, TAR2 for nitrogen and KUP3, KUP4, KUP8 for potassium. Expression studies done using these genes in contrasting genotypes for N and K efficiency raised under field conditions showed significant upregulations and downregulations in their expression for efficient and less efficient genotypes. Allele mining for allelic variations in contrasting genotypes (15S409 and Export kappa) for two genes (AMT2 and NTR3) didn’t show much significant variation. Although SNP differences were observed for NRT3 gene, further functional studies are required to confirm this result. The study identified three main nutrient efficient genotypes viz., KBH18, which was the highest yielder, highest in N efficiency and third highest in K efficiency, 8S501-2, which was second highest in yield, highest in K efficiency, third highest in N efficiency and also exhibited early bulking and 15S409, which was the third highest yielder, second highest in N efficiency & K efficiency. The superior genotypes identified in the present study can be subjected to field trials for confirming their superiority and release as a variety.
