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ThesisItem Open Access Influence of different levels of energy on growth performance of crossbred pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2001) Rekha, P; KAU; George, MathenAn experiment was conducted to assess •the influence of different levels of energy on the growth performance of crossbred (Large White Yorkshire x Desi) pigs. Fifteen male (castrated) and fifteen female weaned crossbred piglets with an average body weight of 12.9 kg were divided into three equal groups as uniformly as possible with regard to age, sex and body weight. The three groups of piglets were maintained on three rations with 16 per cent crude protein but differing in their energy content, viz., 2800 kcal (T1), 3000 kcal CT2) and 3200 kcal (TI) of digestible energy (DE) per kg. The average daily gains recorded for the three groups T1, T2 and TI were 262.9, 302.0 and 362.8 g respectively. The cumulative feed conversion efficiencies were 6.0, 5.2 and 4.1 for the groups T1, T2 and TI respectively. The values recorded for TI were higher (P<0.01) than those for T1 and T2. The digestibility coefficients of nutrients except that of crude fibre and crude protein were found to improve with increase in the energy content of the rations. Study of the carcass traits revealed that the body weight at slaughter and dressed weight without head improved as the energy content of the rations increased. However, dressing percentage, carcass length and back fat thickness were not significantly influenced by the energy content of the ration. The cost of feed per kg weight gain of animals was significantly lower (P<0.01) for the dietary treatment T3 compared to that of T2 and Tl, the values being Rs.49.90, 43.30 and 34.10 for Tl, T2 and T3 respectively. The above results indicate that crossbred pigs require 3200 kcal of DE/kg of the ration for better growth performance provided the crude protein level is maintained at 16 per cent.ThesisItem Open Access Effect of replacing dried fish with lysine and methionine in the calf starter on the growth performance of crossbred calves(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2004) Jith John, Mathew; Ally, KA growth trial was conducted with fourteen crossbred calves below one month of age for 145 days to study the effect of replacement of unsalted dried fish in the calf starter with a total vegetable protein source supplemented with lysine and methionine. Calves were divided into two groups as uniformly as possible and allotted randomly to two dietary treatments. The control group was fed with a calf starter containing unsalted dried fish. The treatment group was fed a calf starter with only vegetable protein sources formulated to be isonitrogenous and isocaloric with control calf starter and equated by supplementation, with the same as regard to amino acids lysine and methionine plus cystine. Statistical analysis of the data showed that there was no significant difference in any of the parameters recorded during the study. An average daily gain of 325 grams and 348 grams were obtained in calves fed with the control and treatment diets respectively. Feed to gain ratio for the control and treatment groups were 3.85 and 3.81 respectively. Dry matter intake and haematological parameters such as haemoglobin, serum phosphorus, serum calcium, plasma glucose, serum total protein and blood urea nitrogen were similar in calves receiving the two dietary treatments. The digestibility coefficients of nutrients observed were 66.37 and 65.84 for dry matter, 72.21 and 72.36 for crude protein, 64.28 and 61.85 for ether extract, 62.34 and 60.71 for neutral detergent fibre and 51.95 and 52.25 for acid detergent fibre for the control and treatment diets respectively. Identical performance was obtained in all the parameters recorded. Cost per kilogram body weight gain was Rs 71.40 for the control group and Rs 69.63 in the treatment group. It could be inferred from the study that unsalted fish in a calf starter can be fully replaced by plant protein sources supplemented for existing deficiencies in lysine and methionine plus cystine to obtain similar growth performance of cross bred pre-ruminant calves.ThesisItem Open Access Marker assisted selection for milk production traits in vechur cattle(Department of Animal Breeding and Genetics, College of Veterinary and Animal Sciences Mannuthy, 2005) Shymaja, Uthaman; Raghunandanan, K VThesisItem Open Access Investigations on etio-pathology of vomiting in dogs(Department of Clinical Medicine, College of Veterinary and Animal Sciences, Mannuthy, 2001) Muraly, P; KAU; Baby, P GThe study "INVESTIGATIONS ON ETIO-PATHOLOGY OF VOMITING IN DOGS" was conducted in 20 dogs to evaluate ultrasonography and radiography as diagnostic tools in vomiting dogs; to assess hydration status, electrolyte and acid-base balance in vomiting dogs and to correlate clinico-pathologic findings with radiographic and ultrasonographic changes. Various parameters such as history, physical examination, hydration status, ultrasonography, radiography-plain and contrast, haematology, serum biochemistry, and wherever possible histopathology were studied. Most of the dogs under study had bile stained watery vomitus but dogs with pyloric stenosis had frothy or watery white vomitus. The frequency of vomiting in dogs with gastritis and gastrointestinal (GI) obstruction was two to seven times per day, it was variable in dogs with hepatic and renal disorders, but was associated with food intake in dogs with pyloric stenosis. Physical examination was found useful in dogs with GI obstruction, while it was non-specific in dogs with gastritis and renal disorders. Capillary refill time (CRT) and degree of sunken eye balls were helpful to assess dehydration. Estimation of volume of packed red cells (VPRC) was found beneficial to assess dehydration unless the dogs are anemic. Ultrasonography could not identify any lesions in dogs with gastritis, but was useful to detect GI obstructions due to pyloric stenosis, intussusception and foreign body and to characterise lesions in the parenchymal organs like liver and kidney. While plain radiographs could give indication to possible non-radiopaque GI obstructions, contrast radiography was required to confirm. Radiography could not identify any lesions in dogs with gastritis, hepatic and chronic intestitial nephritis. Hypokalemia with metabolic alkalosis was the significant electrolyte and acid-base derangement in dogs with vomiting due to gastritis and GI obstructions.ThesisItem Open Access Immunodiagnosis of bovine gastrothylacosis using coproantigens(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 2000) Kandasamy, A; KAU; Devada, KA study was conducted on the prevalence of paramphistomatidosis in Thrissur from June 1999 to May 2000, feasibility of coproantigen detection by ELlSA and comparison of sensitivity of ELlSA using coproantigens and ELlSA using serum antibodies in diagnosis of gastrothylacosis, caused by Gastrothy/ax crumenifer, in cattle. It was noted from the registers maintained at the University Veterinary Hospitals at Kokkalai and Mannuthy and that at the Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, that out of a total number of 1534 faecal samples from bovines examined, 253 (16.5 per cent) animals were found to be positive for amphistome eggs with the maximum prevalence (23 per cent) in June and July. Generally the infection was prevalent throughout the year. An indirect ELlSA using rabbit hyperimmune serum against somatic antigens of G. crumenifer was performed to detect coproantigens in faecal samples collected from 100 known G. crumenifer infected cattle. Seventy four samples were found to contain detectable levels of coproantigen indicating a sensitivity of 74 per cent. Serum samples collected from the same infected cattle were tested for antibodies to G. crumenifer by an indirect ELlSA using somatic antigens. Fifty one samples were found positive for antibodies indicating a sensitivity of 51 per cent. It was seen that when 43 cattle were positive for both coproantigens and serum antibodies, 18 cattle were negative for both of them. Although 31 cattle which were negative for serum antibodies were found positive for coproantigens, eight cattle negative for coproantigens were found positive for serum antibodies. The results showed that coproantigen detection, which revealed a higher sensitivity than the detection of serum antibodies by ELlSA, is feasible for the diagnosis of gastrothylacosis in bovines.ThesisItem Open Access Effect of probiotic supplementation on the performance of broiler chicken(Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy, 2001) Sabitha Mahaboob Kadari, A; KAU; Elizabeth, V KThe effects of different levels of pro biotic (Lactobacillus acidophilus, Streptococcus faecium and Yeasacc 1026) supplementation• at 0.025 and 0.05 per cent of the ration on the performance of broiler chicken were evaluated using 144, one-day old, commercial broiler chicks for a period of eight weeks. The birds were divided into three dietary treatment groups viz., standard broiler ration (T 1), standard broiler ration with 0.025 per cent probiotic (T 2) and standard broiler ration with 0.05 per cent probiotic (T3). Standard broiler ration was formulated as per Bureau of Indian Standards (1992) specification for broiler chicken feed. The 0.025 per cent probiotic supplemented birds showed a significantly higher (P<0.05) body weight upto six weeks of age. At the end of eight weeks of age, the 0.05 per cent probiotic fed birds grew faster. The body weight gain was significantly higher in 0.025 per cent probiotic supplemented group upto six weeks of age but was statistically non-significant upto eight weeks of age. The feed intake was not statistically significant throughout the experimental period. Eventhough the feed efficiency was significantly (P<0.01) better in the group fed with 0.025 per cent probiotic at the end of second week, it was statistically non-significant at sixth and eighth weeks of age. The protein efficiency was not significantly different throughout the experimental period. The serum cholesterol levels were significantly (P<0.01) reduced in both the probiotic supplemented groups. The serum protein level was not affected by probiotic supplementation. The processing Yields did not show any significant difference among treatments. The mortality percentage was not affected by treatments. Cost of production of broilers in the 0.025 per cent probiotic group was lower when compared with other two groups at the end of six weeks of age, while it was lower in the 0.05 per cent probiotic supplemented group at the end of eight weeks of age. It can be concluded that probiotic supplementation in standard broiler ration at a lower level was beneficial in the early stages of growth.ThesisItem Open Access Effect of bakers yeast on growth and nutrient utilization in crossbred (large white yorkshire X desi )pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 2003) Sekar, M; Viswanathan, T VA study was carried out to assess the influence of baker's yeast culture on growth performance, digestibility of nutrients and carcass characteristics in Large White Yorkshire x Desi (75:25) crossbred pigs. Thirty weaned piglets (15 barrows and 15 gilts) with an average body weight of 13.5 kg were divided into three homogeneous groups with regard to age, sex and body weight. Ten piglets in each group were randomly distributed into five replicates of two piglets each. The three groups were randomly allotted to three dietary treatments viz., T,- control (standard ration), Tr control diet supplemented with 0.25 per cent baker's yeast culture and T3- control diet supplemented with 0.5 per cent baker's yeast culture. The piglets were fed on their respective grower rations which contained 18 per cent crude protein and 3200 kcal digestible energy per kg until they attained an average body weight of 50 kg and thereafter changed to finisher rations which contained 14 per cent crude protein and 3 100 kcal digestible energy per kg until the pigs were slaughtered. A digestibility trial was conducted towards the end of the experiment using chromic oxide as external indicator. On completion of seven months of age six pigs from each treatment were slaughtered to study the carcass characteristics. Average daily gain of 352, 383 and 405 g were recorded for the groups T" T2 and T3, respectively. The pigs fed with diet containing 0.5 per cent baker's yeast (T3) had higher (P<0.05) average daily gain than those fed with unsupplemented diet (T,). But, there was no significant difference between T, and T2 as well as T2 and T3. However, there was a trend for a higher average daily gain for those fed 0.25 per cent yeast culture (T2) over those fed control diet Cl (T,). Feed conversion efficiency was 4.36, 4.05 and 3.83 for the groups T" T2 and T3, respectively. The pigs fed with diets containing 0.25 (T2) and 0.5 per cent baker's yeast (T3) had higher (Pwith unsupplemented diet (T,). The digestibility coefficient for dry matter and ether extract were significantly higher (P<0.05) for pigs fed 0.5 per cent yeast culture than those fed with unsupplemented diet and there was no significant difference between T, and T2 or T2 and T3. Pigs fed diet containing 0.5 per cent baker's yeast had higher (P<0.05) crude fibre digestibility than those fed with the diets containing 0 and 0.25 per cent yeast culture. Study of the carcass characteristics revealed that the dressing percentage, carcass length, back fat thickness and loin eye area were not significantly influenced by the inclusion of yeast culture at different levels. The cost of feed per kg live weight gain was Rs.36.63, 35.52 and 34.99, respectively for the treatments T}, T2 and T3. Feed cost per kg live weight gain was lower in T3 compared to other treatments. It is concluded that baker's yeast can be economically included at 0.5 per cent level in the diet for better growth and feed conversion efficiency in crossbred pigs.ThesisItem Open Access Nephroprotective effect of Aerva Lanata (Cherula) and Boerrhavia Diffusa (* Thazhuthama) in rats(Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences,mannuthy, 2003) Fakrudeen Ali Ahamed, N; Venugopalan, KAn experiment was conducted to assess the nephroprotective effect of Aerva lanata (Cherula) and Boerrhavia diffusa (Thazhuthama) against gentamicin induced nephrotoxicity in rats. A group of 48 adult albino rats weighing 150-250 g of either sex were include in six groups and each group consisting of eight rats. All the experimental groups except. saline control (G I) were given gentamicin sulphate (80 mglkg/day subcutaneously) for 12 days. After 12 days, the experimental groups (G3, G4, G5 and G6) were given two selected doses [(G3 and G5 - 250 rng/kgjand (G4 and G6 - 500 mg/kg)] of dried alcoholic extract of Aerva lanata and Boerrhavia difJusa orally, for a period of another 21 days respectively. Feed intake and body weight was reduced in gentamicin administered groups (G2, G3, G4, G5 and G6) at I ih day and it was reverted to normal at 33rd day (G3, G4, G5 and G6). . Elevated blood urea nitrogen (1 ih day) was reverted to normal at 26th day in Aerva lanata @ 250 and -500 mg/kg (G3 and G4) and at 33rd day in Boerrhavia diffusa @ 250 and 500 mg/kg (G5 and G6) respectively. Microscopical examination of urine at 33rd day, revealed the initiation of regeneration of renal epithelium. The severity of gross and microscopical lesions of kidney observed at 12th day was minimized at 33rd day, indicate the nephroprotective effect of Aerva lanata and Boerrhavia diffusa. The change in haemogram at I z" day (reduced total leukocyte count, neutrophil count and increased Iymphocyte count) was reverted to normal in all the groups at 33rd day. Hence the study confirmed that Aerva lanata and Boerrhavia diffusa posses nephroprotective action and it could be used as nephroprotective agents .ThesisItem Open Access Immunopathologic and toxic effects of endosulfan in chick embryo(Centre of Excellence in Pathology, College of Veterinary and Animal Sciences, Mannuthy, 2004) Kalai Selvan, P; KAU; Divakaran Nair, NThe present study was undertaken to investigate the immunopathologic and toxic effects of endosulfan in chick embryo. The embryos were exposed to endosulfan at various dose levels through different routes. The weight of the embryo, bursa, thymus and spleen, histopathology of the lymphoid organs, liver, kidney and brain and quantification of the preformed maternal IgY level in the egg yolk were the parameters analysed to study the effects. Endosulfan was found to be teratogenic at the grven dose levels. The abnormalities observed were agenesis of beak, crossed beak, short lower beak, eventration of viscera and curled toe. Endosulfan did not cause any significant gross changes in the developing embryos except mild haemorrhages. Endosulfan treatment did not produce any significant reduction in the weight of the embryos as well as the weight of the lymphoid organs. On histopathological examinations endosulfan was found to be nephrotoxic and hepatotoxic to the embryos. Varying degrees of degeneration and necrosis was evident in kidney and liver. Effect on the bursa, thymus and spleen were mild to moderate where in the degenerative changes predominated. Dose dependent increase in the involution process of the thymus as evidenced by increase in the Hassal's zone was observed. The lesions in the developing lymphoid organs reflected that endosulfan is toxic to the system and going to affect the immunocompetency by way of immunosuppression and thereby rendering the chicks susceptible to various diseases. The study indicated that the endosulfan treatment did not have any effect on the preformed maternal immunoglobulin level in the egg yolk.
