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Open Access
Combined application of granular preparations of pre-emergent herbicides and fertilizers in rice
(Department of Agronomy, College of Horticulture, Vellanikkara, 1995) Bindu, N K; KAU; John, P S
A field experiment was conducted at the Agricultural Research Station, Mannuthy under the Kerala Agricultural University during the first crop season of 1994 to find out the effect of pre-emergent herbicides and fertilizers in rice. The experiment was laid out in a randomized block design and consisted of fourteen treatments with three replications. Broadleaved weeds and sedges dominated the field. Among broadleaved weeds, Mono choria vaginalis and sphenocleazeylanica were predomint. Shoenoplectus lateriflorus and Cyperus difformis were the prominent sedges present in the field and Echinochloa colonum,saccolepis interrrupta and Isachne miliacea were the important grasses present. Butachlor, anilofos and oxyflourfen under different methods of application reduced the weed Population. Though oxyflourfen recorded high weed control efficiency, it was highly phytotoxic to the rice crop and resulted in poor crop stand. Butachlor and anilofos applied as homemade granular (GP) preparation or as spray alone, or in combination with fertilizer was safe to the crop. The available nutrient in the soil was not affected by the different methods of application of butachlor and anilofos, alone or when combined with fertilizer. Butachlor and anilofos as spray or as GP applied alone, or in combination with fertilizer recorded similar crop growth characteristics, yield and yield attributes. Nutrient up taken by weeds was lower in butachlor and anilofos treatment. Lower weed index values were recorded in butachlor and anilofos treatment. The highest return per rupee was obtained from anilofos treatments. The highest return per rupees was obtained from anilofos treatments. In general, combined application of anilofos or butachlor GP with fertilizer gave efficient control of weed, produced higher grain yields and high return per rupee invested. It also reduced cost, energy and time of weed control operation.
Open Access
Qualitative and quantitative changes in stored rice
(Department of Home Science, College of Agriculture, Vellayani, 1995) Geetha, Roy; KAU; Prema, L
Qualitative and quantitative changes in stored rice varieties viz. PTB – 10 and Red Thriveni were determined by assuming the changes in their grain constituents, physical characteristics, insect infestation, cooking characteristics and organoleptic qualities. After six months storage effect of different storage containers such as gunny bag, pathayam and metal bin on these qualities were taken into consideration. The grain constituents like protein, nonprotein nitrogen, calcium , iron and phosphorous were found to decrease at a greater rate in PTB – 10 stored in gunny bag when compared to red thriveni. Similarly constituents like moisture, uric acid and freefatty acids were found to increase in PTB-10 than in red thriveni. Chnages in grain constituents, reducing sugars, nonreducing sugars, starch and amylose were observed both in raw as well as in cooked rice samples after storage. Increase in reducing sugar and amylose was at a greater rate in PTB-10 stored in gunny bag especially when cooked while nonreducing sugars and starch level were found to decrease in a similar order. Compared to PTB – 10 in gunny bag after storage, red Thriveni obtained lower values for thousand grain weight and total solid level, when stored in all the storage containers tried. Head rice yield and gelatinization temperature of rice samples increased in red thriveni after storage, the rate being higher in gunny bag when compared to other two storage structures. Viscosity was found to increase greatly in red thriveni stored in metal bin. Insect infestation was found to be greater in gunny bags while metal bin was found to be insect proof. PTB – 10 was affected by insects more when compared to Red thriveni. PTB-10 obtained higher values for cooking characteristics such as volume expansion and gruel loss than red thriveni, when stored in gunny bag. As as result of storage water uptake level and swelling index increased at a greater rate in red thriveni in all the storage containers tried. The rate of decrease in optimum cooking time was greater in PTB-10 stored in gunny bag while stickness was greatly reduced in red thriveni when compared to PTB-10. Raw rice samples were more acceptable before storage as quality parameter hardness increased after storage. Storage provided low scores for colour and odour which had a negative influence on consumer acceptance. Red Thriveni was more acceptable for all the quality attributes than PTB-10. The quality attributes colour and appearance obtained low scores in the two rice varieties the decrease being greater in PTB-10 than Red Thriveni. Taste, flavor and doneness was found to decrease in the two rice samples. The rate of decrease in taste. Flavor and doneness was lower in red thriveni when compared to PTB-10. Among the storage containers, metal bin was found to be a better storage structure than pathayam and gunny bag. The experiment threw light on the significant of metal bin as a storage structure and favourable facts in the shelf life qualities of evolved varieties like red thriveni.
Open Access
Optimum plot size for inter croping experiments
(Department of Agricultural Statistics, College of Horticulture, Vellanikkara, 1995) Reji, K; KAU; Sunny, K L
A uniformity trial was conducted in bhindi intercropped with cowpea at the experimental field of College of Horticulture, Kerala Agricultural University, Vellanikkara during July 1993 to December 1993 to assess the nature and magnitude of soil heterogeneity, and to determine the optimum size of plot for increasing the efficiency of experiment with intercropping. Three different approaches have been attempted in the statistical analysis. At the time of harvest, the yield data from 320 plots each of size 0.60m x 0.45m were recorded separately after discarding the border rows. It was observed that the index of soil heterogeneity was very high in all these approaches indicating that the contiguous plots are not correlated and the fertile spots are distributed randomly or in patches. It was also observed that an increase in the plot size in either direction decreased the coefficient of variation but the decrease was not proportional in all these approaches. The empirical law suggested by smith gave a satisfactory fit to the data. All the other non- linear models tried also gave a satisfactory fit to the data in all these approaches. The optimum plot size obtained by following different approaches indicated that 10 basic units plots(2.7m2) are optimum for conducting the intercropping experiment with bhindi and cowpea. Comparison of the LER and the bivariate analysis method showed that for the same level of precision the LER method gives a smaller plot size implying that per unit cost of experimentation will be higher in bivariate analysis. For any choosen plot size the precision attained through LER analysis is larger than that through bivariate analysis. However, when the multivariate approach is adopted the optimum plot size was obtained as 10 basic units (2.7m2) as in the case of LER.
Open Access
Assessment Of Bacteriological quality Of Raw Milk In Trichur And Its Public Health Importance
(Department of Veterinary Public Health, College of Veterinary and Animal Sciences,Mannuthy, 1995) Anju Raghunathrao, Kapre; KAU; Nanu, E
In the present study an effort has been made to assess the bacteriological quality of raw milk obtained from three different sources in Trichur. A total of 21 individual and seven pooled samples were collected from each sources (S1, S2 and S3), over a period of five months. The samples were subjected to different bacterial counts and also for the isolation and identification of S. aureus and E. coli. The isolates were tested for their sensitivity to various chemotherapeutic agents. The average total viable count of individual milk samples from S1, S2 and S3 were 7.5 x 104, 1.4 x 105 and 2 x 105 CFU per ml respectively. Significant difference (P < 0.01) between the counts from S1 and S2; and S1 and S3 was noticed. The average coliform count for S1 was 2.4 x 10, for S2 was 4.8 x 104 and for S3 was 3.8 x 103 CFU per ml. There was significant difference (P < 0.01) between the counts from S1 and S2 ; S1 and S3 ; and S2 and S3. The average counts for thermotolerant coliforms in samples from S1, S2 and S3 were 2.2 x 10, 2.4 x 104 and 2.4 x 103 CFU per ml. The counts from S1 and S2 ; and S1 and S3 differed significantly (P < 0.01). The average faecal streptococcal counts for the sample from S1, S2 and S3 were 1.5 x 102 , 2.1 x 103 and 1.7 x 103 CFU per ml. Significant difference (P < 0.01) between the counts from S1 and S2 , and S1 and S3 was noticed. The staphylococcal counts in samples from S1, S2 and S3 averaged 5.7 x 102, 2.8 x 103 and 6.8 x 103 CFU per ml respectively. Significant differences (P < 0.01) between the counts from S1 and S2 , and S1 and S3 were noticed. The average S. aureus count in samples from S1 was 8.5 x 10, from S2 it was 1.8 x 102 and from S3 , 7.1 x 10 CFU per ml. The average E. coli counts in samples from S1, S2 and S3 were 2 x 102, 1.2 x 104 and 1.5 x 103 CFU per ml respectively. The counts in samples from S1 and S2 ; S1 and S3 ; and S2 and S3 differed significantly (P < 0.01). The average total viable count in pooled milk samples from S1 , S2 and S3 were 4 x 104 , 1.8 x 106 and 2.1 x 105 CFU per ml respectively. Significant difference (P < 0.01) between the counts from S1 and S2 and S1 and S3 was noticed. The average coliform counts at 370C of incubation in the pooled samples from S1, S2 and S3 were 5.5 x 10, 2 x 105 and 6.4 x 103 CFU per ml respectively. The counts from S1 and S2, S1 and S3 ; and S2 and S3 were found significantly different (P < 0.01). The average thermotolerent count in samples from S1, S2 and S3 were 2.8 x 10, 3.6 x 104 and 4.4 x 103 CFU per ml respectively. Significant difference (P < 0.01) in the counts of S1 and S2 ; and S1 and S3 was noticed. The average faecal streptococcal count in samples from S1, S2 and S3 were 2 x 102, 4.8 x 103 and 2.9 x 103 CFU per ml respectively. Significantly different (P < 0.01) counts were noticed between S1 and S2 ; and S1 and S3 was noticed. The average staphylococcal count in samples from S1 was 9.2 x 102 from S2 was 5.3 x 104 and from S3 was 1.3 x 104 CFU per ml. The counts in samples from S1 and S2 ; and S1 and S3 were significantly different (P < 0.01). The S. aureus counts in milk samples from S1, S2 and S3 averaged 1 x 102, 4.8 x 102 and 1.1 x 102 CFU per ml respectively. The average E. coli count in samples from S1, S2 and S3 were 2.7 x 102, 8.9 x 104 and 1.9 x 103 CFU per ml respectively. Significant difference (P < 0.01) between the counts of samples from S1 and S2 ; S1 and S3 ; and S2 and S3 was observed. All the individual samples from S1 were either of very good or good grades (95.24 and 4.76%) respectively. All the pooled milk samples from this source was of very good grade. Most of the individual samples from S2 were of very good or good grades ( 76.20 and 23.80%) respectively, but the pooled milk samples from S2 were of very good, good, fair and poor grades (42.84, 28.60, 14.28 and 14.28%) respectively. Among the individual samples from S3 source all were of either very good or good grades (80.95 and 19.05%) respectively. Pooled milk samples from the same source had very good and good grade (57.14 and 42.86%) respectively. None of the samples from this source were of fair or poor grades. Of the 60 suspected colonies isolated, 54 were identified as S. aureus. Antibiogram of S. aureus isolates showed highest sensitivity to cloxacillin (100%) and gentamicin (100%) followed by amoxicillin (87.03%), chloramphenicol (77.80%) and penicillin – G (35.20%). Of the 70 suspected colonies isolated 66 were identified as E. coli. The E. Coli. Isolates were most sensitive to gentamicin (96.96%) followed by amplicillin (93.92%), furazolidone (80.30%) and carbenicillin (15.155). Doxycycline was least effective drug with no sensitivity and high resistance (90.90%).
Open Access
Pathology of hypothyroidism in pigs
(Centre of Excellence in Pathology, College of Veterinary and Animal Sciences,Mannuthy, 1995) Prasanna, K S; KAU; Sreekumaran, T
An experimental model of hypothyroid state was induced in piglets, using thiourea with the objectives of studying the sequence of clinico pathological changes and its influence on the animal health and growth. Tweleve Large White Yorkshire male piglets of 2 – 3 months age were selected for the study. The animals were divided into control group of six animals and experimental group of six animals. Experimental hypothyroidism was induced by feeding thiourea daily for a period of three months at the dose level of 50 mg per kg body weight. Haemogram, body weight, plasma proteins, serum cholesterol and serum thyroxine values were estimated at periodic intervals. The piglets were subjected to detailed autopsy after sacrifice. Gross lesions were recorded and detailed histopathological examination of tissues was carried out. During the course of experiment all the experimental animals recorded stunted growth and appreciable reduction in feed intake and alopecia of neck and shoulder regions. There was significant increase in blood cholesterol values and plasma protein level in thiourea fed group. A significant reduction in serum thyroxine level was also recorded. There was significant increase in the relative weight of thyroid, adrenal and pituitary glands of experimental animals. Gelatinisation of subcutaneous fat and dilatation of right ventricles were common findings at autopsy. Histologically the thyroid glands exhibited varying degree of hyperplastic changes and depletion of colloid in the follicles. Hyperplasia and hypertrophy of the lining epithelium was also observed. Predominant histological changes in the pituitary was hyperplasia and hypertrophy of the basophil cells and degranulation of the acidophil cells. Adrenal glands showed diffuse hyperplasia of zonafasiculata and accessory cortical nodule formation. Skin revealed acanthosis, hyperkeratosis and keratinisation of harifollicles. In all the hypothyroid animals testis showed varying degree of tubular degeneration. A random survey study was conducted to assess the thyroid status of pigs from different parts of Kerala using serum thyroxine as the marker. This concluded that most of the animals had the normal range of serum thyroxine levels.
Open Access
Microbial degradation of mimosine in goats
(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 1995) Prabhakaran, P; KAU; Devasia, P A
An investigation was carried out to find out the extent of in vitro microbial degradation of pure mimosine (T1) and that of immature leaves (T2), mature leaves (T3), tender stems (T4) and seeds (T5) of L. leucocephala using strained rumen liquor obtained from three rumen fistulated Saanen – Malabari crossbred goats maintained under standard conditions of feeding and management. The proximate chemical composition and mimosine content of different edible parts of leucaena during the months of May, June and July were determined. While immature leaves and seeds had higher crude protein content, seeds had higher crude fat, tender stems had higher crude fibre and mature leaves had higher ash content compared to other edible parts of subabul. The average mimosine concentrations of T2, T3, T4 and T5 were 12.11 + 0.05, 4.89 + 0.02, 3.90 + 0.04 and 10.70 + 0.08 per cent respectively during May; 11.66 + 0.06, 5.23 + 0.03, 3.62 + 0.03 and 10.44 + 0.05 per cent respectively during June and 9.96 + 0.05, 4.92 + 0.03, 3.73 + 0.02 and 9.51 + 0.04 per cent respectively during July on a dry matter basis. The average mimosine concentrations of strained goat rumen liquor incubated with 37.50 mg/100 ml of added mimosine in pure form or as immature leaves, mature leaves, tender stems and seeds showed significant reduction at every 12 hr intervals from 0 to 48 hr of incubation, the final average concentrations being 23.98 + 0.37, 23.14 + 0.37, 22.20 + 0.28, 23.12 + 0.52, 23.35 + 0.37 mg/100 ml of SRL. The percentage of in vitro degradation in respect of T1, T2, T3, T4 and T5 increased significantly at every 12 hr intervals of incubation from 0 to 48 hr, even though the degradation was incomplete with all treatments, the average percentage degradation at 48 hr of incubation being 31.69 + 1.02, 34.49 + 1.18, 37.12 + 0.99, 34.54 + 1.50 and 33.41 + 1.03 respectively. The overall average rate of disappearance of mimosine in µg.ml-1 . h-1 in respect of T1, T2, T3, T4 and T5 for the entire period of 48 hr of incubation were 2.33, 2.54, 2.74, 2.54 and 2.44 respectively with highest rates during 0 to 12 hr, lower rates during 24 to 36 hr and least rates during 36 to 48 hr. The production of ammonia and VFA coincided with the active degradation of mimosine, there being faster degradation upto 12 hr of incubation with highest concentrations of ammonia and VFA at 12 hr of incubation. The overall results indicated that the rumen microorganisms of crossbred goats degrade mimosine to DPH, ammonia and VFA and that mimosine does not inhibit the microbial activity, even though the possible role of leucaena endogenous enzymes in the partial degradation of mimosine recorded in the present study cannot be ruled out.
Open Access
Differential adoption of plant protection technology by farmers of Kerala - a critical analysis
(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 1995) Meera, B; KAU; Thyagarajan Nair, G
Indian agriculture has undergone intensive farming with the introduction of high yielding varieties of crops. Adoption of improved technology and management practices for boosting up production of food grains have resulted in serious pest and disease complexes warranting intensive and extensive use of plant protection chemicals. A number of limitations and adverse side effects such as pest resistance to pesticides, pesticide residues, health hazards, environmental pollution and ecological imbalance have been identified recently. The components of plant protection technology were taken to study the differential adoption in important crops like paddy and vegetables by the farmers and explained the variations in their cognitive, affective and connative components of behaviour with a selected set of independent variables. The study analysed the indigenous practices of plant protection being followed by farmers and also their perception about the impact of pesticides on environmental aspects. The major constraints experienced by the farmers in the adoption of plant protection technology along with the suggestions to overcome the same were also studied. The study was undertaken in two districts of Kerala viz., Thiruvananthapuram and Alappuzha. A sample of 120 farmers each from the two districts was selected and the total sample size for the study was 240.The data were collected using an interview schedule and analysed using suitable statistical techniques. There was significant difference among the farmers of Thiruvananthapuram and Alappuzha districts with regard to their knowledge about chemical and cultural methods of plant protection. Majority of farmers of both the districts belonged to low knowledge group. The farmers of both the districts were ignorant about biological, physical and integrated methods of plant protection methods. Majority of the farmers of both the districts possessed favourable attitude towards chemical method of plant protection. The farmers of Alappuzha district were significantly higher adopters of plant protection methods than the farmers of Thiruvananthapuram district. Majority of the farmers of both the districts belonged to low perception category with regard to their perception about the utility and practicability of plant protection methods. Crops yield index and scientific orientation emerged as significant independent variables in the correlation and multiple regression analysis with regard to the farmers of both the districts. The practices, viz., controlled application of nutrients for reducing pest and disease attack in paddy and use of ‘thulsikeni’ in ‘pandals’ to trap and kill fruit flies in vegetables were judged as the most effective and scientifically rational practices adopted by farmers. The farmer’s perception about the impact of pesticides on environmental aspects was very low and majority of them belonged to low perception category. The constraint ‘untimely supply and high cost of imputs’ was the most important constraint experienced by the farmers of Thiruvananthapuram district, while ‘lack of proper drainage facilities’ was the most serious constraint experienced by the farmers of Alappuzha District. The most important suggestion given by the farmers to overcome the constraints was to ‘develop simple and more compatible plant protection technologies’ and the most important suggestion given by the experts to overcome the constraints experienced by the farmers was to ‘impart adequate training to farmers as well as extension workers on IPM practices’. The strategic model developed by integrating the salient findings of the present study, emphasized the importance of popularizing effective plant protection technology among the farmers in ecologically sensitive rice and vegetable production systems in Kerala.
Open Access
Development of subsurface pad irrigation system for tomato
(Department of Agronomy, College of Agriculture, Vellayani, 1995) Abdul Nassar, K; KAU; Jaikumaran, U
An experiment was conducted in the summer rice fallows of the Agricultural Research Station, Mannuthy during 1995 to develop and test subsurface pad irrigation system for tomato and to compare it with surface irrigation. The soil was sandy clay loam, medium in organic carbon and available potassium and high in available phosphorus. The eight treatments comprised of combination of four frequencies of irrigation (IW/CPE ratios of 1.2, 0.9, 0.6 and 0.3) and two irrigation systems (subsurface pad irrigation and surface irrigation). The experiment was laid out in randomized block design with three replications. Poly bags filled with saw dust placed 45 cm beneath the surface at the frequency of one pad for every four plants formed SSPI. Water was supplied to pads by laterals supplying 40 mm of water per irrigation. The study revealed that tomato responded very well to irrigation. Biometric characters like plant height, number of leaves and leaf area index and yield attributing characters like number of flowers, number of fruits and total weight of fruits per plant were favourably influenced by frequent irrigation under both the systems of irrigation. The fruit yield increased with frequency of irrigation and maximum at the IW/CPE ratio of 1.2. The plants irrigated by surface method grew taller, had more leaf area index, produced more number of green leaves, flowers, fruits and total fruit weight per plant compared to the plants irrigated under SSPI system at the irrigation frequencies of IW/CPE ratios 1.2, 0.9, and 0.6. But at the IW/CPE ratio of 0.3, the subsurface irrigated plants performed better than the surface irrigated plants both in terms of growth and yield attributing characters. The crop receiving irrigations through SSPI systems consumed lesser amount of water at all the frequencies of irrigation compared to surface method. This decline at the IW/CPE ratios of 1.2, 0.9, 0.6 and 0.3 were to the tune of 22,30,40 and 18 per cent respectively. The soil moisture extraction from 0-15, 15-30 and 30-60 cm layers in SSPI was 62-70, 24-29 and 6-12 per cent respectively whereas in surface irrigation the respective values were 62-64, 22-23 and 13-15 per cent. The soil moisture redistribution was rapid in the case of surface irrigation whereas it was gradual in the case of SSPI. Moisture content in the case of SSPI was maximum at the radial distance of 15 cm from the pad on either sides whereas in surface irrigation there was no remarkable difference in the moisture content with respect to radial distance from the plant.
Open Access
Response of Gladiolus to Rapid Cloning Through in Vitro Techniques
(Department of pomology and floriculture, College of horticulture,Vellanikkara, 1995) Sakkeer Hussain, C T; KAU; Geetha, C K
Investigations were carried out to study the response of gladiolus to rapid cloning through in vitro techniques at the Department of Pomology and Floriculture and Plant Tissue Culture Laboratory of All India Co-ordinated Floriculture Improvement Project, College of Horticulture, Vellanikkara, during 1992-94. The main objective was to identify the most suitable explant and media combination for in vitro cloning. The explants used were corm axillary buds, cormel tips, inflorescence nodal segments (for enhanced release of axillary buds), inflorescence internodal segments, flower buds, flower bud bracts, root segments (for somatic organogenesis) and leaf segments (for somatic embryogenesis). The best season for the collection of corm axillary buds and cormel tips was from September to May. Surface sterilization of the explants could be effectively done with 0.1 or o.2 per cent mercuric chloride and the duration of treatment varied from I to25 minutes. Culture establishment of the corm axillary bud, cormel tip explants were better in MS medium supplemented with BAP ranging from 1.0 mg 1 -1 to 4.0 mg 1-1. The concentration of BAP required varied according to the stage of development of corms and cormels. Higher levels of BAP was ideal during early stages of development of corm and cormels. Of the different media (White’s, SH and MS) tried, MS medium was found to be the best culture establishment (Stage 1) when supplemented with 3.0 mg 1-1 BAP. Elongated shoots of Stage I were subjected to shoot proliferation (Stage 2). Multiple axillary bud production was very high when the MS medium was supplemented with BAP 1.0 mg 1-1 and NAA 0.5 mg 1-1 or BAP 2.0 mg 1-1 and NAA 0.5 mg 1-1. Callus production from the base of the elongated shoots were observed when the concentration of NAA increased in the medium. Of the different cytokinins (BAP, kinetin and 2ip) tried, BAP was found to be the best in Stage 2. Frequent subculturing onto the MS medium containing BAP 2.0 mg 1-1 and NAA 0.5 mg 1-1 increased the production of multiple axillary buds. These when transferred to the MS medium devoid of growth regulators resulted in elongation of shoots. The elongated shoots produced maximum number of roots in the MS medium containing 1.0 mg 1-1 IBA under the exclusion of light. However, early rooting was obtained in MS liquid medium devoid of growth regulators. Plantlet survival was maximum when treated with 0.2 per cent Bavistin immediately after removing from the culture vessels, followed by treatment with 0.2 per cent mancozeb and norfloxacin at the time of transplanting and post planting treatment with 1/10 MS solution and drenching with triadimefon 20.0 mg 1-1 at three days interval inside improvised mist chamber. Direct organogenesis could be obtained from immature inflorescence segments in modified MS medium supplemented with 15.0 mg 1-1 NAA and 3.0 mg 1-1 BAP. Among the various explants tried for callus mediated organogenesis, callus index was the maximum (400) when immature inflorescence segments were inoculated to the modified MS medium supplemented with NAA 15.0 mg 1-1 in 16 h photoperiod and also in the medium supplemented with 15.0 mg 1-1 NAA + 2.0 mg 1-1 BAP and kept under exclusion of light. The callus derived from inflorescence segments differentiated into shoots in the MS medium supplemented with 3.0 mg 1-1 BAP and also in the medium supplemented with 1.0 mg 1-1 BAP and 0.5 mg 1-1 NAA. Callus also could be obtained from flower buds and flower bud bracts. The callus derived from the corm axillary buds and cormel tip explants in Stage 2, differentiated in the basal MS medium devoid of growth regulators or supplemented with 20.0 ml 1-1 coconut water and also in the medium with 0.5 mg 1-1 BAP. The root segments (both in vitro and in vivo) produced callus in MS medium supplemented with 1.0 mg 1-1 NAA and the differentiation was obtained in the medium containing 3.0 mg 1-1 BAP an 1.0mg 1-1 NAA. Leaf segments failed to develop callus. However, the explants collected from the leaf covering the inflorescence boot leaf) when cultured in modified MS medium supplemented with 15.0 mg 1-1 NAA and 1.0 mg 1-1 BAP and incubated under darkness for three months developed somatic embryos. In vitro corm production was noticed in the cultures, if planting out was delayed. Earliest and large sized corm induction was made possible in elongated shoots of gladiolus from Stage 2 in Ms medium containing 5.0 per cent sucrose, 0.5 mg 1-1 NAA and 5.0 mg 1-1 triadimefon kept under etiolated condition. The size of the in vitro produced corms enlarged from 0.2 cm to 2.3 cm in the MS liquid medium containing 5.0 per cent sucrose and 3.0 mg 1-1 triadimefon.

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