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Open Access
Preparation of mozzarella cheese using skim milk filled with coconut milk
(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1994) Gnana Selva, Johnson; KAU; Mukundan, M
A detailed study was carried out to determine the quality of coconut fat filled milk for the preparation of Mozzarella cheese and why drinks. Literatures based on filled milk products has been reviewed, apart from the preparation of cheese and why drinks. The control samples of Mozzarella cheese and whey drinks were prepared using cow’s milk. Experiment I products were prepared from milk in which 50 per cent of milk fat was replaced with coconut fat. Experiment II products were prepared from cheese milk in which 100 per cent of milk fat was replaced with coconut fat. All the samples of milk were standardized to 4 per cent fat. A total of 6 trials were carried out to obtain reliable data for statistical analysis. The acidity, pH, stretchbility and FDM content were found to be similar in control, experiment I and II Mozzarella cheese. Eventhough, the control Mozzarella cheese were found to have slightly higher yield protein, fat and lower moisture content, the experimental I and II. Mozzarella cheese also satisfied the requirements for good quality Mozzarella cheese. The control Mozzarella cheese got maximum score on sensory evaluation than the experiment I and II Mozzarella cheese. Pineapple and Lemon falvoured control, experiment I and II whey drinks were found to be equally acceptable with nodifference on storage studies ar 5 + loC. Total bacterial count on whey drinks were also made. The studies revealed that the cow milk in which the milk fat replaced to the extend of 50 per cent and 100 per cent with coconut fat can be effectively utilised for preparation of Mozzarella cheese. The quality of such cheese is comparable with that made from cow milk.
Open Access
Metabolic profile of downer cow syndrome
(Department of Clinical Medicine, College of Veterinary and Animal Sciences,Mannuthy, 1994) Mhachuvino Catherine, Khatsu; KAU; Alikutty, K M
The metabolic profile of ‘Downer Cow’ syndrome in field conditions was studied. Fourteen field cases of ‘Downers’ in crossbred dairy cows aged three to thirteen years, ranging from 250 to 300 kg body weight from Trichur district were selected at random and utilized for the study. Fourteen apparently healthy crossbred dairy cows of similar age group and body weight, maintained under similar conditions of feeding and management from the area from which the clinical cases studied were also selected at random and utilized as the healthy controls. Samples of blood for haematological and biochemical parameters, urine and dung from both healthy and diseased animals were collected and analysed using standard methods. Analyses of the data from fourteen diseased animals indicated a higher incidence in Jersey crossbred cows during summer season. Prominent clinical signs were sternal recumbency exhibiting hindquarter weakness and reduced feed and water intake. However, the affected animals remained bright and alert with no evidence of any systemic disturbances. The clinical data were within physiological limit. Highly significant increase in PCV and significant increase in Hb but no significant difference in ESR, RBC and WBC were observed. Lymphopenia, neutrophilia and eosinopenia were observed with no variation in basophils and monocytes counts. Biochemically, hypocalcaemia,hypophosphataemia, hypoproteinaemia and hypoalbuminaemia were obtained from ‘Downers’ with no significant variation in blood glucose, urea nitrogen, sodium, potassium, magnesium and albumin/globulin ratio. Urinalysis revealed no consistent result indicative of any systemic involvement and no parasitism on dung examination microscopically.
Open Access
Effect of dried spleen as growth stimulator in kid rations
(Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 1994) Shyama, K; KAU; James, C S
An investigation was carried out to assess the effect of dried spleen as a growth promotant in kid rations. Twenty four female Malabari kids with an average body weight of 8.5 kg were distributed randomly and as uniformly as possible into four groups (groups 1, 11, 111 and IV) of six animals each, with regard to age and weight. The four dietary treatments A, B, C and D were allotted to the kids in the groups 1, 11, 111 and IV respectively, ration A with a concentrate mixture containing 16 per cent crude protein supplemented with dried buffalo spleen at the rate of 0.1 per cent, ration B forming the control diet at 16 per cent protein level without incorporation of spleen, ration C with a concentrate mixture containing 12 per cent crude protein supplemented with dried buffalo spleen at the rate of 0.1 per cent, ration D forming the control diet at 12 per cent protein level. Jack leaves formed the sole source of roughage to the animals. The experimental duration was 120 days. The results indicated that incorporation of dried spleen in the ration enhanced the growth performance of animals, especially in animals maintained on low level of protein, as evidenced by the cumulative and average daily weight gain registered in that descending order being 7.52 kg and 62.64 g, 7.4 kg and 61.67 g, 6.88 kg and 57.36 g and 5.00 kg and 41.67 g for the animals maintained on rations C, A, B and D respectively. The efficiency of feed and protein utilisation also exhibited the same trend. It was also noticed that, a cost of production per kilogram gain of Rs.32.58 and 26.14 were registered for animals maintained on spleen incorporated rations (rations A and C) as against Rs.35.08 and 38.63 respectively for the animals maintained on corresponding control groups (ration B and D) indicating a better cost efficiency in spleen incorporated groups, especially in animals maintained on low level of crude protein in the ration. The haematological studies reveal that R.B.C., W. B. C., haemoglobin, plasma protein, plasma calcium, phosphorus and magnesium were within the normal range prescribed for the species. The animals maintained on ration C showed a significant increase (P < 0.01) in plasma calcium level when compared to that of the control. Digestibility studies reveal no significant difference in digestibility of dry matter and nitrogen free extract between the four groups. Animals maintained on the spleen incorporated rations (groups 1 and 111) showed better digestibility (P < 0.01) of crude protein and ether extract than the respective control groups (groups 11 and IV). Incorporation of dried spleen could not bringforth any influence on fibre digestion. The results of the balance experiment showed better retention of nitrogen, calcium, phosphorus and magnesium in the animals maintained on spleen incorporated diets. The higher retention of these parameters could be substantiated by the comparatively lower faecal and urinary excretion of these, in animals maintained on spleen incorporated rations (rations A and C). An overall assessment of the results, indicated that incorporation of dried buffalo spleen in the ration of goats, at the rate of 0.1 per cent, certainly augment the nutrient utilisation by increasing the digestibility and retention, and can be recommended as a harmless natural growth promotant with economic benefit.
Open Access
Antigens of pasteurella multocida isolates from rabbit and their immunologencity
(Department of microbiology, College of veterinary and animal sciences, Mannuthy, 1994) Manoharan S; KAU; Jayaprakasan, V
Two rabbit strains viz. R9 S and R23 S and a bovine vaccine strain P – 52 which were maintained in virulent form, were used for the preparation of three forms of antigen viz., heat inactivated crude extract, KSCN extract and sonicated antigen. These antigens were chemically analysed for protein and carbohydrate contents and were found to be higher in the sonicated antigen preparation irrespective of the source. In SDS – PAGE analysis, the profiles discerned by heat inactivated crude extract, KSCN extract and sonicated antigens were four, five and six protein bands with molecular weights lesser than 68 kDa while the KSCN extract and sonicated antigen presented an additional protein band with molecular weight higher than 68 kDa. Three types of antigen of P. multocida were characterized and analysed for the inter relationship and the immunogenic potential in mice. Antiserum was raised against each antigenic preparation from the three strains in rabbits and used for serological study. In AGPT and immunoelectrophoresis the serum developed multiple precipitin lines and arcs respectively when reacted against the three homologous and two heterologous antigens in which a few were identical to the heterologous antigens. The results revealed stronger serological relationship between the two rabbit strains than with the cattle strain and the heterogeneity of the sonicated antigen. The antibody titre in each antiserum was measured by IHA using the sensitized GA – SRBC/T – GA – SRBC and the titres were more in the homologous antiserum and high titre for the heterologous serum was seen with the sonicated antigen. The LD50 determined for the three strains R9 S, R23 S and P- 52 was found to be 3 x 104 , 3 x 103 and 3 x 105 bacteria. Immunogenic potential of the three antigens and an adjuvanted sonicated antigen were tested in mice by giving two doses of vaccine at 14 days interval and challenging on 21st day with homologous and heterologous strains. A higher percentage of protection was conferred by homologous strains and it was cent per cent (100%) with sonicated antigen. The percentage of protection against challenge with heterologous strains was low. An elaborated study on immunity trials with these immunogens is needed before recommending the R23 S as a candidate vaccine strain.
Open Access
Identification of bacterial critical points and antibacterial effect of lactic acid on beef carcass
(Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 1994) Rajeev, K; KAU; Soman, M
During the process of slaughter and subsequent processing, the beef carcass is exposed to bacterial contamination. Bacterial load is one of the parameters for assessment of the sanitary conditions in slaughter operations. It is tedious and time consuming to evaluate bacterial load of carcass surface as a whole. Therefore assessment of bacterial load on certain points (critical points) in the carcass which are more frequently exposed to contaminants will help in quick assessment of sanitaty standard. In the present study six critical points were selected on beef carcasses to evaluate the bacterial contamination. Carcasses from two slaughter houses differing in infrastructural facilities were used for this assessment and comparison. There was significant difference in the level of bacterial contamination on critical points of carcasses obtained from the two sources. Significant difference was noticed between points as well. Among the critical points, neck-lateral has shown highest level of contamination. This may be due to chances of exposure to contaminants during bleeding and flaying. The abdomen-medial was comparatively less contaminated. In spite of conscious precautions, carcasses invariably get contaminated. In order to minimize the bacterial load on carcass at the end of slaughter line, washing carcass with sanitizers is one of the methods adopted in meat trade. Lactic acid one percent solution, when used as sanitizer for washing beef carcasses immediately after slaughter, has shown significant reduction in total viable count, coliform count and faecal streptococcal count estimated 1 h after treatment. When hot lactic acid solution at 700C was used for washing, significant reduction in the above counts in comparison to the first treatment was observed. This added benefit can be attributed to the enhanced temperature of the solution. It is concluded that one percent lactic acid solution, preferably at 700C, can be effectively used as a sanitizer on beef carcass surface for reduction of initial bacterial load and this helps in prolonging the storage life under the retail marketing condition.
Open Access
Modified intra-dermal test for the diagnosis of paratuberculosis in goats
(Department of Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, 1994) Vinod Kumar, K; KAU; Saseedraranath, M R
Efficacy of single intra – dermal Johnin test was compared against two modified intra – dermal Johnin tests for diagnosis of paratuberculosis in goats. One hundred and fifty adult goats were divided at random into three groups and each group was subjected to one of the intra - dermal tests. Group 1 being the single johnin intra – dermal (SID) Johnin test whereas in group 11 (modified intra – dermal test – MID – 1) and group 111 (modified intra – dermal test – MID – 11). The second injections were given after third and fifth days of first injection. Six animals giving positive results and two animals giving negative results were slaughtered from each group and confirmation of infection was ascertained by histopathology. Fecal samples and rectal pinch from all the animals were subjected to zeihl – Neelsen’s acid – fast staining for detection for M. paratuberculosis. Serum magnesium levels of all test positive animals and an equal number of test – negative animals from each group were compared. After 24 h MID – 1 was found to be superior to both SID and MID – 11 tests. The MID – 1 test had higher specificity (66.6 per cent against 58.33 per cent in SID and 40 per cent in MID – 11) and predictive value of positive results (83.3 against 55.5 in SID and 50 per cent in MID – 11). The MID – 1 and MID – 11 tests had 100 per cent sensitivity and predictive value of negative results as against 50 per cent and 33.3 per cent of SID. Examination of fecal sample and rectal pinch was found to be of little value in the diagnosis of early paratuberculosis in goats. Serum magnesium levels between test positive and test negative animals was found to be not significantly different in all three groups. The results of the present study indicate that MID – 1 test could be used as an efficient diagnostic test for detection of paratuberculosis among goats.
Open Access
Factors influencing calf growth and mortality in field and organised farms
(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 1994) Shaji, Antony .; KAU; Thomas, C K
A study was undertaken to evaluate the system of calf raising in the field vis – a – vis that in organised farms, which included contemporary calves born in field and organised farms. Mavelikkara was chosen as the study area from where the experimental field calves were selected. University Livestock Farm, Kerala Agricultural and Dhoni Farm, KLD Board, Palghat were chosen to represent organised farms in the experiment. All the calves born in the experimental area or farms during a period of two months were included in the experiment. Calves were studied upto period of six months of age. It was observed that climatic conditions in general were similar at the three locations except that at ULF, Mannuthy relative humidity and hours of bright sunshine tended to be less than the other two locations. The male calves in the farms weighed heavier at birth and gained faster to weigh significantly more at the 12th fortnight compared to those in the field. There was no significant difference in birth weight of farm and field born female calves. However, those in the farms grew faster to weigh significantly heavier at 12th fortnight. When male and female calves in the farms and field were put together and tested at 1st, 6th and 12th fortnights, the calves in the farms weighed significantly heavier than those in the field at all stages. The daily weight gain during different fortnights was higher in the farm grown calves compared to their counterparts in the field. There was no significant difference in height of male calves in the farm and field in the 1st fortnight but was significantly higher in the farm grown calves in the 6th and 12th fortnights. There was no significant difference in the height of the female calves in the farms and field in all the three fortnights tested. Information with respect to girth showed similar trend as in the case of weight and height. In the first fortnight, the male calves in the farms measured significantly shorter in length than those in the field but by 6th and 12th fortnights, they measured significantly longer than male calves in the field. There was no significant difference in length of female calves in the field and farms during the time intervals tested. In the field, calves received generally an adequate amount of colostrum through suckling. Those in the farm were fed 2 – 2.5 kg colostrum daily from the pail. The interval between birth and first nursing varied from 15 min to 2 h in the field. In the farms, calves born during day time received colostrum within half an hour, while those born in night received colostrum the next morning. Highest daily gain and lowest mortality was seen in calves fed colostrum within 15 minutes. It was observed that the quantity of milk available to female calves in the field was comparable to that in the farms. Male calves in the field received much lesser quantity. Female calves received more quantity of concentrates compared to males except in the early fortnights. Maximum daily gain of calves was observed in sheds with concrete flooring and tiled roofs. Monthly deworming was practised upto 12 months of age in the two farms studied. In the field all calves were dewormed during the 1st month only. Thereafter, female calves were given more frequent deworming treatment. Vaccination against Foot and Mouth disease was carried out in the farms but not in the field. Good hygiene and daily grooming increased the daily gain of calves. Better growth was seen when the animals were looked after by men than women. Average dairy awareness was observed in 90 per cent of the farmers. Daily gain of calves increased with increased dairy awareness. People with high school level education maintained their calves better than those with higher or lower levels of education. Calves grown by agricultural labourers seemed to have lower weight gain. Calves reared in larger units of land had a greater daily weight gain. The mortality rate among calves was 14 per cent in the field compared to 8.5 per cent in the farms. Mortality rate of 66.6 per cent was seen no colostrum was fed. There was no mortality among calves fed colostrum within 15 minutes. Male calves had higher mortality rate than female calves. Higher mortality rate was observed in calves born to first calvers. In field mortality was higher in 1st and 5th month of age. Mortality rate was lower when calves were looked after by men compared to women. The lowest calf mortality rate was observed among calves owned by those who had high school level education. Mortality rate among calves seemed to decrease with increasing dairy awareness of owners. Mortality rate of calves was highest in shed and premises with poor hygiene (50%) followed by medium hygiene (11.11%) and good hygiene (10.5%). Similarly daily grooming seemed to reduces mortality rate (11.9%) compared to weekly grooming (25%). At the end of 6 months, 92.85 per cent of the female calves were retained by the farmers compared to only 36.36 per cent male calves, clearly pointing towards the greater future economic utility of the former.
Open Access
Synchronisation of oestrus super ovulation and embryo collection in goats
(Department of Animal Reproduction, College of Veterinary and animal Sciences, Mannuthy, 1994) Benjamin, E D; KAU; Nair, M S
With the object of evolving effective methods for standardising techniques for synchronisation of oestrus superovulation and collection of embryos 18 healthy goats were selected from the goat farm attached to the College of Veterinary and Animal Sciences Mannuthy and randomly divided into three different groups with six animals in each Animals m the first group were given two doses of 10 mg PGF2cl H days apart and those in the second group were given 12 5 mg progesterone injection daily for 16 days Six animals in the third group were not given any treatment and kept as control The respective treatments were repeated in group I and II after a period of sixty days and superovulation in group I was carried out with eCG injection 1000 IU given intramuscularly on the day previous to the second dose of PGF2a In group II superovulation was done by intramuscular injection of 1000 IU eCG given on the 15th day of progesterone treatment All the animals in the above groups and animals in the control group were inseminated with good quality buck semen four to six hours after the onset of oestrus Embryos were collected surgically m all the eighteen goats by flushing the fallopian tube towards the fimbria after conducting laparotomy of the inseminated goats on the left flank All the animals in group I came to oestrus 57 8 ± 5 65 h after the second injection of PGF2a and the duration of oestrus was 48 ± 8 76 h In group II 83 33% were m oestrus at an interval of 101 6 + 6 11 h after the last progesterone injection and the duration of oestrus was 28 ± 1 41 h The results of administration of eCG in the second treatment regime with PGF2a in group I revealed that all animals in this group evinced oestrus at a mean interval of 50 3 ± 10 86 h after the second injection of PGF2a and the mean duration of oestrus was 44 ± 4h The total number of ovulation points on both the ovaries in this group were 8 4 ± 1 94 with 4 ± 1 30 and 4 4 ± 0 748 for the right and left ovaries respectively The total number of unruptured follicles on both the ovaries was 5 33 ± 1 64 The animals in group II after administration of eCG and progesterone evinced oestrus 72+9 06 h after the last progesterone injection with the duration of oestrus as 38 3 ± 4 46 h The total number of ovulation points on both the ovaries was 12 8 ± 1 4 and the values were 7 5 ± 2 31 for the right and 5 3 ± 2 04 for the left ovary The total number of unruptured follicles on both the ovaries was 3 2 + 1 579 The results of embryo collection in animals in group I revealed that the average number of embryos collected from both the ovaries was 4 8 ± 0 97 with 2 2 ± 0 66 for the right and 2 6 ± 0 39 for the left ovary The total number of embryos collected from all the animals in both the ovaries was 24 (57 14%) of which 17 (70 83%) were transferrable
Open Access
Postnatal Development of the Oviduct in the Japanese Quail (Coturnix coturnix japonica)
(Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1994) Lucy, K M; KAU; Harshan, K R
The structure and postnatal development of the oviduct in quails were investigated using 72 birds aged from day-old to 60 days. The growth, morphology and histology of the oviduct were studied using six birds at each age group. In the day-old quail chick, the oviduct could seen as a narrow white translucent tube towards the left side of the coelom connected by dorsal and ventral ligaments. The sign of coiling was evident from 25 days of age. In the initial stages, the increase in weight and length of oviduct was in accordance with the growth of the bird. Rapid changes in the development of the organ occurred between 30 and 4 0 days of age and a spurt in growth was noticed from 40-60 days of age. In the day-old chick, the cranial regions of the oviduct corresponding to the infundibulum, magnum and isthmus were undifferentiated. Throughout the length of the oviduct, histological appearance was the same. The mucosa was thrown into low primary folds lined by simple columnar epithelium and there was subepithelial connective tissue containing densely packed cells with fine collagen and reticular fibres. The large number of luminal epithelial cells and the increase in height of the mucosal folds indicated entry into a rapid growth phase which started between 30 and 40 days of age. In the adult bird, the innermost epithelium of the different segments of the oviduct consisted of ciliated columnar cells and secretory goblet cells. Lamina propria was packed with tubular glands except in the funnel of infundibulum, magnum-isthmus junction and vagina. Bundles of collagen and a few elastic and reticular fibres constituted the core of the mucosal folds. Mucosal folds also contained lymphocytes in diffuse form and nodular aggregates. Thickness of the tunica muscularis increased from the anterior to the posterior end of the oviduct. Externally there was a typical serosa

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