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ThesisItem Open Access Genetic studies in red gram (eafanui caiaixL)(Department of Agricultural Botany, College of Horticulture, Vellanikkara, 1988) Radhakrishnan, V V; KAU; Narayanan Namboodiri, K NThesisItem Open Access Deterioration of oil cake by fungi(Department of Plant Pathology, College of Agriculture, Vellayani, 1989) Naseema, A; KAU; Wilson, K IFungi causing deterioration of coconut, groundnut and sesamum oil cakes were studied. ficremonium implicatum, Asperdllus aculeatus, A. flavus, A. fumigatus, A. nlaer, A. terreus, A. versicolor, Bipolaris hawaiiensis, Curvularia clavata, Monascus ruber, Penicillium aurantioqriseum, P. Pinophilum, Pestalotiopsis palmarum, Rhizomucor £usillus and Ehizopus stolonifer were obtained from coconut oil cake. Aspergillus flavus, A. niaer, A. terreus, A. versicolor, Gliocladium sp. Penicillium pinophilum, RhizoEUS or^zae and Rhizopus stolonifer were noticed in groundnut and Aspergillus candidus, A. flavus, A. fumigatus, A. nlaer, A. tamarii, A. terreus, Curvularia clavata, Eurotium. chevalieri, F"sarium pallidoroseum, Monascus ruber, Fenicilliuiu pinophilum, Pestalotiopsls palmarum and Rhizopus or^zae in sesamum oil cake. Of these, Acremonium implicatum, Aspergillus aculeatus, A. caeslellus, A. .f"-igatus, Bipolaris hawaiiensis, Curvularia clavata, Monascus ru^, Penicillium anrantlogriseum, P. pinophilum, Pestalotiopsls palmarum and Rhizomucor pusillus from coconut oil cake, Aspergillus versicolor, Gliocladium sp., Penicillium pinophilum, Rhizopus oryzae and R. stolonifer from groundnut and Aspergillus candidus, A. fumigatus, A. tamarli, A. terreus, Curvularia clavata, Eurotium chevalieri, Fusarium pallidoroseum,Monascus ruber, Penicillium pinophilum, Pestalotiopsis palmarum and Rhizopus oryzae from sesamum oil cake have not been reported earlier. * Aspergillus flavus and A. niger were isolated from all the samples of groundnut and sesamum oil cakes. In coconut oil cake, these two fungi were present in 88.89 and 77.78 per cent of the samples. A. terreus was isolated from 66.67 per cent of groundnut and 55. 56 per cent of coconut and sesamum oil cake samples. Penicillium pinophilum was obtained from 66.67 per cent of groundnut, 44.44 per cent of sesamum and 27.78 per cent of coconut oil cake samples. Wide variation was noticed in the population of fungi present in the oil cakes collected from different regions during different periods of the year. Oil cakes collected during June-July had the highest population, of fungi. The central and the northern regions recorded higher population of fungi than the southern region. Positive and significant correlation could be obtained between weather parameters and population of fungi in different oil cakes. Maximum correlation was noticed in relation to total rainfall. Qood mycelial growth of fungi was obtained in all the oil cakes incubated at 27, 29 and 32°C. Maximum mycelial growth was noticed at 100 per cent relative humidity. This was followed by 96.1 per cent and 92.9 per cent in the descending order. The oil content of the oil cakes was considerably reduced due to the growth of all the fungi tested individually and in combination. Maximum reduction v/as noticed due to the growth of Pestalotiopsis palmarum in coconut oil cake, Rhizopus stolonifer in groundnut and Fusarium pallidorosem in sesamum oil cake. In the case of combinations, Aspergillus flavus, A. niger and Penicillium pinophilum together caused maximum reduction in oil content of coconut oil cake. In groundnut, combined growth of A. flavus, A. niger and A. terreus caused maximum reduction in oil whereas, A. niger and P. pinophilum together effected maximum reduction of oil in sesamum oil cake. Oil cakes inoculated with different fungi showed considerable reduction in total carbohydrates, crude protein, free amino nitrogen, crude fibre and ash to the extent of 6.11 to 76.95 , 4 . 28 to 68.03, 14.91 to 92.52, 1.25 to 92.55 and 0.17 to 65.16 per cent respectively. In the case of mineral nutrients like phosphorus, potassium, magnesium. calcium, copper and iron reduction ranging from 15.07 to 75.54, 23.13 to 94.41, 10.89 to 63.37, 28.78 to 90.20, 52.52 to 97.12 and 0.32 to 60.77 per cent respectively was noticed. Fourteen out of 2 0 isolates of Aspergillus flavus produced aflatoxins B^, and G2 in culture medium with maximum quantities being 1210, 1040 and 151 ppb respectively by the isolates from coconut oil cake. Eight out of 19 isolates of A. niger elaborated upto 222 ppb by the isolate from sesamum oil cake. When grown on the respective host material, A. flavus isolates from coconut oil cake produced maximum quantity of B^^, B^ and being 1517, 1092 and 272 ppb respectively. A. niger isolate from coconut oil cake produced B^^ upto 419 ppb. oil cakes treated with calcium propionate (0.6 per cent, w/w) were free from fungus growth throughout the period (180 days) of observation and showed minimum number of fungal propagules whereas, those kept as control had higher population of fungi than the treated ones, at all period.of observation. Oil cakes stored in polythene lined gunny bags had the least population of fungi, whereas those stored in ordinary gunny bag had very high population of fungi. These results revealed that fungal deterioration and spoilage of oil cakes could be prevented or reduced to the minimum by treatment with 0.6 per cent calcium propionate- and by using polythene lined gunny bags for storage arid transport.ThesisItem Open Access Nutrient dynamics if the rice based cropping systems(Department of Soil Science and Agricultural Chemistry, College of Agriculture, Vellayani, 1989) Sundaresam, Nair C.; Subromonia, Aiyer, RThesisItem Open Access Homeostatic analysis of components of genetic variance and inheritance of fruit colour, fruit shape and bitterness in bitter gourd (Momordica charantia L.)(Department of Olericulture, College of Horticulture, Vellanikkara, 1989) Abdul Vahab, M; KAU; Gopalakrishnan, P KThesisItem Open Access In vitro studies on the propagation of cardamom (Elettaria cardamomum maton)(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1989) Reghunath, B R; KAU; Gopalakrishnan, P KThesisItem Open Access Effect of phorate applied for the control of bunchy top vector of banana Pentalonia nigroneroosa Coq. on the plant and in the soil environment(Department of Agricultural Entomology, College of Agriculture, Vellayani, 1989) Sitarama Rao, D; KAU; Mohandas, NA series of experiments were carried out for ascertaining the basic problems related to the current recommendations for managing bunchytop disease of banana through the application of phorate. The absorption, translocation and metabolism of phorate applied in the soil was influenced more by the condition and age of the plant than by the dose of the insecticide. Since the application of 2.50 g ai/plant did not result in corresponding increase in the residue content or the bioefficacy in the early phases of crop growth, when compared to the 1.25 dose, the latter can be used without significant loss in efficacy. A definite dose-effect relationship existed between the phorate content of plant and the mortality of P.nigronervosa confined at feeding sites. The median lethal doses of the insecticide content of the plant tissue were higher during declining phase of absorption as compared to those obtained during the active absorption phase. The result indicated the lesser toxicity of some components in the total residue during the later phase of the crop. Application of phorate granules in leaf axils was less effective than the treatment done in the soil and hence the current recommendation to use less quantity of insecticide when applied in the leaf axils has to be altered. A simple technique for the separation, identification and quantification of phorate and its metabolites was developed. Phorate and phorate sulfoxide contents of the total residue showed inverse relationship with each other while the other metabolites did not exhibit a clear relationship among them. Phorate and phorate sulfoxide exhibited more positive direct influences on the morality of the vector than the other metabilites. The absorption and toxicity of the insecticide content in plants did not vary significantly up to 174 DAP, when applied @ 2.50 g ai/plant at different intervals after planting. Absorption was very low when the insecticide was applied at 180 and 210 DAP. For ensuring residues within tolerance limits (0.10ppm) in raw fruits, the insecticide treatment has to be limited to 150 DAP and for ripe fruits the limit can be extended up to 180 DAP. The absorption and persistence of phorate and metabolites was significantly higher in plants grown in summer season than in those grown in rainy season. The absorption of insecticide was high in sandy soils and it was lowest in black cotton soils during the active absorption phase. The insecticide persisted at effective levels for 75 days in sandy, 90 days in lateritic upland and 105 days in black cotton soil. Sulfoxidation of the thioether moiety was the dominent metabolic pathway in sandy soil while desulfuration pathway was predominent in the other three soils. Application of phorate at planting, @ 2.50 g ai/plant, did not adversely affect the soil microflora as observed at the time of harvest of the crop.ThesisItem Open Access Effect of plant protection chemicals on foliar pathogens and Phylloplane microflora of rice(Department of Plant Pathology, College of Agriculture, Vellayani, 1989) Gokulapalan, C; Chandrasekharan Nair, MThe present investigation was undertaken to assess the effects of plant protection chemicals on the important fungal pathogens and phylloplane microflora of rice. An attempt has been made to identify potential biological control agents to combat sheath blight disease causing havoc to rice cultivation in Kerala. Both under pot culture conditions and during the course of field trials at two locations viz., Adoor and Karamana, Kerala, the fungicide carboxin was found to be the best treatment for reducing the incidence and intensity of sheath blight and sheath rot diseases of rice. The fungicide was found to be the least harmful to the epiphytic microflora of the rice plant. In some instances the population of phylloplane antagonists of R. solani, including Trichoderma harzianum, T. viride, Penicillium oxalicum and Aspergillus aculeatus were found to be enhanced by the application of carboxin. The total disease incidence and yield loss were significantly reduced by the application of edifenphos. The rice variety Karthika was found to be significantly tolerant to sheath blight and sheath rot compared with the rice variety Jyothy. The fungicides carboxin and mancozeb at 500ppm did not inhibit the growth of the phylloplane antagonists of R. solani viz., Trichoderma harzianum and T. viride under in vitro conditions. Several micro-organisms isolated from the rice phylloplane were found to exhibit in vitro antagonism towards R. solani. These include Aspergillus aculeatus, A. niger, Chaetomium globosum, Penicillium oxalicum, Trichodema harzianum, T. viride, several bacteria and a few basidiomycetous yeasts. The phylloplane antagonists, Trichoderma harzianum, T. viride and Penicillium oxalicum were found to readily parasitise R. solani hyphae leading to coiling, penetration followed by disintegration and death of the mycohost. When these antagonists were cultured on bran and tried for their efficacy as potential biocontrol agents of R. solani, it was found that these fungi could significantly reduce the incidence and intensity of sheath blight of rice, though this was not comparable with the effect of the fungicide carboxin.ThesisItem Open Access Genic manipulations in sweet potato adopting induced mutations(Department of Agricultural Botany, College of Agriculture,Vellayani, 1989) Suma Bai D I; Krishnan Nair NAn experiment was conducted at the Department of Agricultural Botany, College of Agriculture, Vellayani during 1987-1989 for genetic manipulations in sweet potato through gamma ray induced mutagenesis for increased variability and to isolate out genotypes having wider adaptability and better performance. Stem cuttings of 8 to 10 cm length bearing two nodes each, taken from fifteen sweet potato varieties were used for radiosensitivity analysis. Gamma irradiation was done by a 60 Co gamma cell unit installed in the Radio Tracer Laboratory of Kerala Agricultural University, Trichur. The material was subjected to exposure of 2-10 kR at intervals of 2 kR. The chosen dose rate was 0.162 MR/h. The direct effect of doses on the material was assessed on the basis of days to start sprouting, days to complete sprouting, sprouting percentage, vine length, branch and tuber number and weight of tubers per vine. The exposures above 4 kR caused lethality in the majority of the varieties and hence comparative analysis for ratiosensitivity was assessed at the 2 kR level. The gamma ray exposed population started sprouting late. The days taken for completion of sprouting were also more in all the varieties. Gamma rays, in addition, reduced the sprouting percentage. The percentage lethality varied depending on variety. The vine length and number of branches per vine also varied from variety to variety. They were found to be comparatively less in treated population. The tuber number and weight of tubers per vine were found to be significantly increased by gamma irradiation at 2 kR. Based on the above observations the fifteen varieties were classified into three, viz. low, medium and high radiation sensitive categories. Induced mutagenesis was done in continuation with the radiosensitivity analysis using three varieties, each selected from the low, medium and high radiation tolerant groups. The planting materials selected for gamma irradiation included fresh cuttings, rooted cuttings and rooted tubers which were exposed to radiation at a range of 500 – 2500 r, at 500 r intervals. The dose rate was 0.162 MR/h. The irradiated materials along with the control were planted on the subsequent day. In vM1 generation the direct effect of gamma rays was assessed based on days taken to start sprouting, days taken to complete sprouting, sprouting percentage, lethality on the 30th day of planting and at harvest, vine length, branch number per vine, fresh weight of vine, tuber number per vine, weight, length, girth and volume of tuber and tuber yield per vine. From vM1 plants 3-4 noded cuttings were taken from the basal, middle and top portions for raising vM2 generation. VM3 and vM4 generations were also raised in the same manner. In vM2, vM3 and vM4 generations the yield parameters were analysed in detail. Classification of the phenotypes and frequency analysis were also done. The salient findings of the experiment are the following: There was a delay in sprount initiation and for completion of sprouting caused by gamma ray exposure. A decrease in sprouting percentage and an increase in lethality were noticed under higher levels of exposures. Similarly a reduction in vine length and branch number per vine were found at higher exposures. The fresh weight of vine was reduced and the tuber number increased at higher exposures. There was an increase in mean tuber weight, length, girth, volume and tuber yield per vine at higher exposures. All the exposures and the different modes of treatment induced phenotypic variants both in negative and positive directions. Positive variants were in higher frequency in later generations. Irradiation of rooted cuttings was found to be more economical or beneficial compared to fresh cuttings and rooted tubers. The study enabled to isolate out two promising types, one each from S5 and Bhadrakalichuvala. These mutants outyielded the control and are being multiplied by vine cuttings for farm trials in different agroecological milieus of the State.ThesisItem Open Access Phosphorus management in a rice based cropping system(Department of Agronomy, College of Agriculture, Vellayani, 1989) Annamma, George; KAU; Sasidhar, V KIn order to standardize an appropriate phosphorus management practice in a rice based cropping system involving rice-rice-cowpea/sesamum, field experiments were carried out in the rice fields of the Instructional Farm, College of Agriculture, Vellayani from June 1984 to September 1986. The experiment was laid out in a randomized block design with three replications. There were eight treatments. The treatments comprised of (1) continuous phosphorus application to all the three crops in the system (2) phosphorus application to the first and second crops of rice (3) phosphorus application to the first crop of rice and third crop of cowpea/sesamum (4) phosphorus application to the first crop of rice only (5) phosphorus application to the second crop of rice and third crop of cowpea/sesamum (6) phosphorus application to the second crop of rice only (7) phosphorus application to the third crop of cowpea/sesamum only (8) control plot with no addition of phosphorus to any of the crops in the system. The salient findings of the experiment are as follows: Phosphorus application had no significant influence on grain and straw yield of first crop of rice. But available nitrogen, available and total phosphorus and available potassium of the soil were increased with phosphorus application. Direct, residual and cumulative effects of phosphorus had no significant influence on grain and straw yield of second crop of rice. Phosphorus uptake could not show any variation due to the different treatments. Available and total phosphorus content of the soil were highest under cumulative phosphorus treatment. All the growth and yield attributes of third crop of cowpea and sesamum were increased by the direct and cumulative effects of phosphorus. Grain yield of cowpea was significantly increased by the direct application of phosphorus. Eventhough not significant the highest sesamum yield was accorded by the direct and cumulative application of phosphorus. Phosphorus uptake in all the growth stages of the crop was highest in direct phosphorus plots. Available and total phosphorus content of the soil was highest in continuous phosphorus applied plots. There was no significant influence on grain and straw yield of first crop of rice after cowpea and sesamum in the direct, residual and cumulative effects of phosphorus. Residual phosphorus was sufficient to maintain the available nitrogen status of the soil. Available phosphorus of the soil was increased by the direct, cumulative and continuous application of phosphorus and total phosphorus by continuous application of phosphorus. Balance sheet of available phosphorus revealed that the soil phosphorus level almost maintained, where phosphorus was applied only to the third crop of cowpea or sesamum. The highest net return and benefit-cost ratio for the rice-rice-cowpea and rice-rice-sesamum system was obtained when phosphorus was applied only to the third crop in the rice fallow and the residual effect being utilized by the succeeding rice crops.
