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  • ThesisItemOpen Access
    Studies on Capon Production
    (Department of Poultry Science, College of Veterinary Science, Mannuthy, 1986) Jyotirmoy, Chakraborty; KAU; Sabarinathan Nair, R
    The thesis incorporates the results of a feasibility study on economic utilization of Austra-White and White Leghorn surplus cockerels for capon production in the existing Indian conditions. Production performances of surgically caponized Austra-White and White Leghorn males were compared with their respective intacts at three stages, namely, 20th 24th and 28th week of age. A total number of 192 cockerels were involved in the study. Each treatment group comprised 12 birds and replicated four times. Caponization was carried out at 8 weeks of age. The production straits evaluated were body weight gain, feed efficiency, processing losses and yield, percentage ‘slips’, taste panel evaluation, proximate composition of meat and economics of production. Recordings of thermal data, weekly body weight and weekly feed consumption were made. At the 20th 24th and 28th weeks 16 birds randomly selected from each group were subjected to slaughter studies. The results indicated significantly (p<0.05) higher body weight gain for the capons over intact controls at 20 weeks. Austra-White capons were significantly (p<0.05) higher gain at the 24th week but White Leghorn capons did not differ significantly from their intacts at this stage. Capons and intacts did not differ significantly at 28 weeks of age. Feed efficiency was significantly (p<0.05) better for capons during all the periods under tests. The capon registered significantly (p<0.05) higher dressing loss than intacts whereas intacts registered significantly (p<0.05) higher loss due to head and shanks. Ready to cook yield did not differ significantly between treatments. Capons registered increased percentages of moisture, protein and other extract. Five per cent of capons were registered as ‘slips’. Austra-White capon meat top scored in tenderness, juiciness, and personal preference. Economic evaluation registered profitable returns for capons at 20th week, based on culled/spent hen meat value at university farm rates. Significantly higher returns were indicated when economic evaluations were made on market rates. From the results of the study it was concluded that Austra-White surplus cockerels can be effectively utilized for capon production and White Leghorn cockerels form small sized capons. Capons production can be an economic practical feasibility in Indian conditions and also capon meat is preferred over roaster meat. Surgical caponization can be effectively employed as a routine managemental procedure for economic utilization of surplus cockerels. It was also indicated that the optimum economic stage for marketing capons is 20 weeks of age.
  • ThesisItemOpen Access
    Biology of neoplastic cells of ethmoid carcinoma
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1985) Madan Sing, Karki; KAU; Rajan, A
    Attempts were made to propagate the ethmoid carcinoma cells in vitro to study the biological behaviour of the neoplastic cells and their relationship with the host. Efforts were also made to transplant the neoplastic cells in laboratory animals and tumour bearing natural hosts so as to lay out a model system for further investigation. In vitro propagation was tried by obtaining the tumour tissues from nine cows and three goats. Cells were grown in Hank’s balanced salt solution (HBSS). TC – 199 and Dulbecco’s modified Eagle’s medium with 10 to 20 per cent calf serum. The cell suspension was seeded into tissue culture bottles, milk diluting bottles and test tubes and incubated at 370 C. No growth was observed in the cultures made from the tumour tissue of cows. The tumour cells from two goats grew in monolyerand were maintained for nine days. There was no difference in growth of cells in different media employed. Insulin supplementation in the media did not cause any difference in the growth of the cells. Subsequently the cells died even after subculturing and changing of the media. Absence of certain unknown factors required by the neoplastic cells for growth was considered responsible for failure of the growth. Transplantation studies were carried out in autologous, homologous and heterologous species of animals using the tumour tissue framgments, homogenate, filtrate and cell suspension. No autograft or heterotransplant were successful. Homologous transplantation resulted in a firm painless growth at the site of injection. The growth initiated a cell – mediated immune response at the site of implantation. Failure of neoplastic cells to grow in the other hosts was considered to be due to rejection of the transplants by the host’s immune system.
  • ThesisItemOpen Access
    Regional anaesthesia of the hind-limbs in oats using lignocaine hydrochloride
    (Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1985) Prahlad, Sapkota; KAU; Raveendran, S
    A study on intravenous regional anaesthesia (IVRA) of the hind – limbs was conducted in 18 bucks, weighing 15 to 25 kg body – weight (in four groups). Lignocaine hydrochloride 2% colution was used as the anaesthetic. Four dose levels viz., 4 – 5 mg; 5 - 6 mg; 6 – 7 mg and 7 – 9 mg/kg body – weight were employed. A tourniquet was applied around the limb above the stifle joint. After a 10 min period of pre – injection tourniquet ischaemic, the anaesthetic was administered through saphenous vein following exsanguination. Anaesthetic effect was ascertained by pin – pricking and pinching the interdigital space. In nine animals the effect of IVRA on wound healing was studies. The onset and duration of anaesthesia and the time for waning away of anaesthesia were also recorded. Onset of anaesthesia was noticed in 2.50 + 0.84; 2.0 + 0.82; 2.86 + 1.34 and 2.43 + 1.40 min. Duration of anaesthesia in the four groups was 25.0 + 7.77; 24.86 + 3.80 ; 21.43 + 6.08 and 30.71 + 4.92 min respectively including a short duration after the release of tourniquet. The complete disappearance of anaesthetic effect was noticed by 6.17 + 4.54; 4.28 + 1.38 ; 5.43 + 2.07 and 3.86 + 1.21 min after the release of tourniquet. The anaesthetic effects were first apparent at the phalangeal region and progressed gradually upwards to the level of tourniquet and waned away in the reverse order. The healing of wound was uneventful and the histological study revealed that there was no variation in the healing process between the experimental and control groups.
  • ThesisItemOpen Access
    Chloral hydrate for general annaesthesia in goats
    (Department of Surgery, College of Veterinary and Animal Sciences,Mannuthy, 1981) Viswanathan, R; KAU; George, P O
    Thirtysix apparently healthy alpins – malabari cross- bred bucks aged from 17 to 30 months and weighing from 22 to 39.5 kg were used for the present study. They were divided into two groups viz., Group I and II, consisting of 18 animals in each. Each of these groups was further divided into three subgroups viz. A, B and C, consisting of six animals in each. Freshly prepared chloral hydrate six per cent solution was administered intravenously at the rate of 1.0, 1.5 and 2.0 ml per kg bodyweight, while triflupromazine hydrochloride (Siquil) at the rate of 0.2 mg per kg bodyweight was also administered intravenously as the premedicant , in group II. Following were the salient results obtained during the present study: When the dose of chloral hydrate was increased, 1) Disappearance of palpebral reflex was quicker and duration was longer, 2) Setting in of incoordination of movements was quicker and the time for assuming the normal gait was longer and 3) Recumbency was seen only in higher doses. Administration of triflupromazine hydrochloride (siquil) as premedicant , increased the efficiency of anaesthetic effect in these animals. Temperature variation consequent on the administration of chloral hydrate, with and without premedication , was not significant the variation being less than 10 P. There was an increase in the rate of respiration and pulse within five minutes of administration of the drug (s), which showed a gradual downward trend. There was reduction in the erythrocyte count, haemoglobin and packed cell volume, while there was slight increase in the leucocyte count. On the basis of the present study, intravenous administration of chloral hydrate, six per cent solution, at the rate of two ml per kg bodyweight, may be recommended in goats, for surgical procedures of about half an hour durations. Premedication with triflupromazine hydrochloride at the rate of 0.2 mg per kg bodyweight would give better results.,
  • ThesisItemOpen Access
    Role of parrots in the epizootiology of newcastle disease
    (Department of Microbiology, College of Veterinary and Animal Sciences,Mannuthy, 1981) Vijayan, V; Sulochana, S
    The incidence, susceptibility, mode of infection and duration of excretion of the new cattle disease virus in the common Indian parrots (psittacula Krameri) were studied in detail. The blood, cloacal and throat swabs of parrots, collected from different parts of the State were screened for the presence of ND antibodies and virus. Seventeen out of 103 blood samples were found to possess HI antibodies. The serum samples which gave positive HI titres belonged to parrots kept as pets in households and allowed to mingle freely with domestic poultry and those housed in Trivandrum Zoo along with pigeons. None of the 70 cloacal and 42 throat swabs were positive for the virus. Experimental infection of parrots with undiluted virulent virus by the intranasal and intraocular routes and by the subcutaneous and intranasal routes with 1:100 dilution of the same virus gave almost the same results. All of them died within a weeks’ time, after showing symptoms of inappettance, leg and wing paralysis and diarrhoea, from day two of infection. Virus could be isolated from the throat and cloacal swabs and also from the tissue of dead birds. Chicks kept along with these infected parrots did not develop symptoms of ND, eventhough they excreted the virus, for a few days, and had a low titre of HI antibodies in their sera. All the contact chicks died of ND with typical symptoms and lesions on challenge with the virulent virus. The parrots that received a mesogenic strain (Komorov) of the virus, also succumbed to the disease, but with less pronounced symptoms and with an extended incubation period. The parrots that were infected with lentogenic strain of the virus (F1) did not develop symptoms of the disease. However multiplication of the virus occurred in these birds as isolations could be made from cloacal and throat swab and a slight increase in H1 titre was noticed sera. However on challenge with a virulent strain of NDV, they showed symptoms of ND. All of them died within eight days and the virus could be isolated from them. Contact infection of parrots from infected chicks were quite effective, as the parrots died with the same symptoms described above, almost within the same time as direct infection. Virus was also isolated from the tissues of the dead parrots. The common Indian parrots were found to be highly susceptible to both velogenic and mesogenic strains of NDV, but they were resistant to the lentogenic strain. Uninfected chicks kept along with the parrots infected with virulent virus picked up the infection, and virus could be isolated from the cloacal and throat swabs of these chicks. They also showed an increase in the antibody titre. The failure to produce clinical disease in chicken might be attributed to a decrease in virulence of the virus on passage in parrots. The carrier state with the lentogenis strain of the virus could not be assessed as they were challenged after 2 weeks. Though a carrier role had been attributed to the parakeets imported from India, the parrots in this study were found to succumb to the disease within a week. This might be due to the variation in the susceptibility of various species of parrots to NDV. The chances of dissemination of the virus could be prevented by quarantining them for a period, not less than ten days.
  • ThesisItemOpen Access
    Bacteriological quality of goat milk
    (Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1985) Chakhesang, Kevesiei; KAU; Sukumaran, M V
    A total of 376 aseptically – drawn milk and 46 farm pooled milk samples were collected from the AICRP on Goats for Milk, Mannuthy and subjected to various tests to determine the bacteriological quality. An increase of over 59 and 188 – fold in bacterial number from aseptically – drawn milk to production – and milk for the morning and the evening milkings respectively was noticed. In aseptically – drawn milk samples 61.17% and 64.83% gave a SPC of below 100/ml in the morning and evening respectively. In farm pooled milk samples the SPC exceeded 105/ml in 39.13% of the morning and 65.22% of the evening samples. No psychrophilic bacteria was detected in any of the aseptically – drawn or farm pooled milk samples. The mean thermophilic counts of aseptically – drawn as well as farm pooled milk samples was less than one/ml. Though the mean coliform count of aseptically – drawn milk was found to be less than one/ml, the farm pooled milk samples showed 3,413 and 2734/ml for the morning and the evening samples respectively. The mean MBRT of the morning and the evening samples was 14.91 and 8.93 h for aseptically – drawn milk and 4.57 and 3.83 h for farm pooled milk samples respectively. The correlation coefficient between MBR time and SPC of farm pooled milk of the morning and evening samples was not significant (- 0.21 and - 0.27). The keeping quality of the morning and the evening milk samples (280 C) obtained was respectively 50.48 and 44.70 h for aseptically – drawn milk and 12.87 and 12.04 h for farm pooled milk samples respectively. The correlation coefficient between SPC and keeping quality of farm pooled milk samples was significant (- 0.46) for morning sample while that of evening milk was not significant (+ 0.28). The correlation coefficient between MBRT and keeping quality of farm pooled milk samples was also not significant in both the morning and the evening (+ 0.31 and + 0.13).
  • ThesisItemOpen Access
    Utero-tubal insufflation technique for diagnosis of tubal impatency in cows
    (Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1981) Athman, K V; KAU; Neelakanta Iyer, C P
    The objective of the present study was to design an apparatus for utero-tubal insufflation test and to standardize its use in both excised genitalia and in live animals for the diagnosis and treatment of tubal impotency. Perfect obturation of the cervix was obstained using a stainless steel catheter with 22 FR. Neo Foley’s latex catheter sleeved over. Unilateral and bilateral impotency were recorded in 8.70 and 19.25 per cent of the excised genitalia. The incidence of unilateral and bilateral impotency in 37 infertile animals were found to be 2.70 and 13.52 per cent respectively. The mean pressure at which air escaped from both the tubes in excised genitalia was 128.12 mm. Hg. In cows and 148.93 mm. Hg. In heifers. On the contrary in experimental animals the corresponding pressure in cows and heifers were 116.38 mm. Hg. And 127.50 mm. Hg. Respectively. It was observed that a higher pressure was required to open both the tubes in heifers than in cows. Blown up condition of the uterus took place in excised genitalia of cows and heifers between 100-280 mm. Hg. And 100-260 mm. Hg. Respectively. The pressure range for producing “blown up” condition in experimental animal was found to be 120-220 mm. Hg. In cows and 195-200 mm. Hg. In heifers. Since the safety margin was estimated to be 60.00 mm. Hg. In experimental cows, the test is considered to be a safe diagnostic test for tubal impotency. Although uterus might be “blown up” in a very few cases at a pressure lower than the higher pressure for escape of air from normally patent tubes, the test will not endanger the life of the animal. Since 69.56 percentage of cows with bilateral patency of salpinx on testing conceived after insemination, utero-tubal insufflation test could also be exploited for the treatment of temporary tubal blockage.
  • ThesisItemOpen Access
    Study on the influence of cattle keeping on the bacteriological quality of domestic well water
    (Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 1981) George Oommen, T; KAU; Soman, M
    Wells are the main sources of water supply in Indian villages and towns as elsewhere in any of the rural areas of the developing tropical countries. The shallow, open, dug wells are liable to contamination from the surrounding sources by various means. Faecal contamination of water is considered as one of the major causes of health hazards resulting in innumerable diseases among people throughout the world. Therefore, the bacteriological analysis of water is aimed at the detection and enumeration of indicators of faecal pollution. The movement of animal excreta into surface and ground water is considered a major factor contributing to the bacteriological pollution of available water in many regions. In order to assess the influence of cattle keeping on the bacteriological quality of domestic well water in Mannuthy area, the present study was undertaken. Forty wells from households randomly selected, with and without cattle keeping, for the study spreading over for a period of six months covering the summer and south-west monsoon during 1981. Water samples were collected aseptically, once in each season. They were bacteriologically analysed for SPC, MPN of coliforms and the detection and enumeration of E.coli. FS and C1 perfringens. Bacterial counts in water from all wells far exceeded the standards recommended for open, shallow wells. Although an apparently higher SPC in water from households with cattle keeping than from those without was observed, it was not statistically significant. The seasonal variation in the counts showed a hundred-fold increase in the case of SPC during monsoon than summer. But the similar increase in the MPN was negligible. The indicator bacteria, especially E.Coli and C1.perfringens showed a significant reduction during monsoon. The sanitary survey of the wells conducted with the help of a ready made performa revealed that none of the wells could be considered satisfactory either in construction or maintenance. The SPC and FS counts were found to have a positive correlation with the depth of wells. The SPC was positively correlated with the type of latrine also. The MPN of coliform and the depth of lining and distance of cattle shed from well were negatively correlated; so also a negative correlation was observed in the case of FS count and the distance between latrine and well. The various characteristics of wells, cattle keeping and latrines were found to have a significant correlation with the SPC and MPN of coliforms. When these of wells and latrines were alone considered, they showed significant correlation only with FS count. But it was observed that the characteristics of wells and latrines alone could explain for the considerable variations in the SPC and MPN than those of cattle keeping, as a source of pollution. It is concluded that the type of construction of well do not have any direct bearing on the bacteriological quality of well water except in the case of Cl. perfringens counts. Cattle keeping has also no significant influence on the bacteriological quality of domestic well water in Mannuthy area though all wells under study showed evidence of recent or past faecal contamination.
  • ThesisItemOpen Access
    Growth and carcass characteristics of pigs maintained on rations containing different levels of dried tapioca chips
    (Department of Animal Nutrition, College of Veterinary and Animal Sciences,Mannuthy, 1981) Sasikala Devi, K A; KAU; Thomas, C T
    An investigation was carried out to assess the growth rate, feed efficiency, carcass quality and economics of production in swine with isoproteimic rations containing dried tapioca chips in partial or complete replacement of maize. Eighteen large White Yorkshire weanling pigs were divided into three groups (Group 1, 11 and 111) of six animals each as uniformly as possible in regard to litter, sex, age and weight and maintained on three isoproteimic diets A, B and C containing 0, 20 and 40 per cent levels respectively of dried tapioca chips in partial and complete replacement of maize. Records of feed intake, fortnightly body weights and body measurements were maintained throughout the course of the experiment. Digestibility coefficients of nutrients in the rations were determined. Two males and two females each from all the three groups were slaughtered when they attained the body weight of 70 kg for collecting data on carcass characteristics. Haematological studies were also carried out to assess the nutritional status of the animals. The animals under all the three dietary treatments recorded almost similar growth rates, the overall average daily gains being 385.9, 368.0 and 380.2 g respectively for the three groups 1, 11 and 111. The total food intake of the animals were similar indicating that the three rations, in addition to being isoproteimic, were also isocaloric and of almost equal palatability. The animals also showed similar feed conversion efficiency, the overall average values being 3.59, 3.62 and 3.57 respectively for the groups 1, 11 and 111. The digestibility coefficients of nutrients in the three rations were similar indicating that dried tapioca chips was as efficiently utilised as maize. The normal and similar values for blood constituents indicated that all the animals maintained normal nutritional status and inclusion of tapioca at levels as used in the present study did not exert any deleterious effect on the health of the animals. Results of studies on carcass characteristics revealed that the dressing percentage without head, carcass length, back fat thickness, loin – eye area and percentages of prime cuts against live weight were all found to be similar with both tapioca and maize based diets. The diets used did not seem to markedly influence the melting point of body fat though there was significant difference between subcutaneous and leaf fat indicating a greater unsaturation of subcutaneous fat. Saponification values were found to be similar with all the three diets. With respect to both subcutaneous and leaf fat, iodine number was higher on maize based diets though there was significant difference only in regard to subcutaneous fat. Cost of production per kg live weight of the animals in the three groups 1, 11 and 111 were shown to be Rs.8.20, 8.19 and 7.99 respectively. An overall evaluation of results clearly indicated that dried tapioca chips can be safely and profitably incorporated in swine rations at a level of 40 per cent in place of conventional cereal grains like maize.