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  • ThesisItemOpen Access
    Studies on certain gastro-intestinal nematodes with special reference to those found in goats
    (Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sathianesan, V; KAU; Kalyanasundaram, R
    Life history of Triohuris globuloss has been elucidated for the fist time using kids !q as experimental animals. Both free living and parasitic stages have been described in detail with illustrations. The prepatent period of Triohuric globuloss was found to be 85-89 days. T.globulosa failed to develop in guniea pig on experimental transmission. Haematology and histopathology of trichuriacis in kids experimentally infected with T.globulosa have been studied for the first time and heavy infection with the worm was found to cause anaemia and pathology changes in cacum of the host. Prepatent period of Strongyloides napillosus in kids infected orally with 7000 larva has been determined for the first time to be 6-7 days. Neoasoais vitulorum of calves has been found to be not transferable to oat either transplacentally or orally. In gunia pigs M.Vitulorum failed to attain sexual maturity. Calves could not be infected with infected with M.vitulorum post-nataly. Oesphagostomum colubianum of goats has been found to be not transferable to calves. Prepatent period of O.colusbionum in kids infected experimentally was found to be 40 days. Comparative efficacy of 4 anthelminties viz., Oxibendazole, thiophanate, Albendazole and Parbendazole was increased by conducting treatment trials. Against monospecific infection of Trichuris globulosa in experimentally infected kids the efficacies of Oxibendazile, thiophanate, Albendazole and Parbendazole at the recommended doses of 10mg.50mg, 10mg and 30 mg/kg body weight respectively were found to be 48.6%, 41%,47.3% and 49.3% respectively. Even at double the recommended doses the anthelminations were found to be well tolerated by the kids. Oxibendazole and Albendazole at the rate of 10mg and 10mg/kg body weight respectively were found to be 100% found to be 100 % effective against colubriformise, effective against respectively were Haemonohus contortue, trichostrongylus .Thiopanate at the recommended dosage (59mg/kg body weight) was also 100% effective against all he nematodes mentioned above except Haemonous contortus against which it was only 97% effective at that dosage. Parbendazole at the recommended dosage shedule (30mg/kg body weight) was 100% effective against the nematodes with the exception of .against which only 86.6% efficacy was noticed. Against in nature nematodes in the gastro-intestinal tract Oxibendazole and Albendazole were 100% effective whereas the efficacies of P`arbendazole and thiophanate were 85% and 74% respectively at their recommended doses. In in vitro studies the anthelmintic efficacy of Oxibendasole against Haemonchus contortus was found to be superior to that of the remaining anthelminties. In ovicidal property also, Oxibendazole was better than all the others, since it was ovicidal in 6 hours after administration whereas albendazole, thiophanate and Parbendazole were ovicidal in 8 hours, 9 hour and 11 hours respectively. Albendazole was found to be superior to al the other 3 anthelmintion in their influences on the body weight gain of treated animals closely followed by Oxibendazole. Perbendazole and thiophanate were inferior to others Judging on the basis of over all efficacies Oxibendazole and Albendazole were found to be superior to thiophanate and Parbendasole. Treatment trials with Oxibendazole, Albendazole and thiophanate against gastro-intestinal nematodes of goats have not been attempted earlier than the present study.
  • ThesisItemOpen Access
    Studies on the common ticks affecting live-stock in Kerala
    (Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Rajamohan, K; KAU; Kalyanasundaram, R
    An investigation was conducted for a period of 5 years from 1974 to 1979 in to the incidence of ticks on domestic animals in Kerala, the biology of the most common species, and their susceptibility to four commonly available organo phosphorus compounds.. Nine different species of ticks belonging to 5 genera were collected from cattle, buffaloes, goats and dogs in different localities of the eleven districts of Kerala. The species of ticks identified were boophilus annulatus, rhipicephalus sanguineus, R.haemaphusaloides, R.turanicus, haemaphysalis bispinosa, H. turturis, H.spinigera, Hyalomma anatolicum and Amblyomma integrum. The commonest among them was boophilus annulatus, which was found mainly on cattle. The incidence of this tick was very high in the hilly areas and in the western ghat regions. The other common ticks found on cattle were Rhipicephalus haemaphysaloides and Haemaphysalis bispinosa. Tick infestation was found to be rare in buffaloes and goats and the common species found on them were Haemaphysalis bispinosa and B. annulatus. IN dogs the species recorded in the present study were Rhipicephalus sanguienus and R. turanicus. Variations in incidence was evidenced from the present study. The maximum incidence was found in the months of July, August, September, October and November. The life-cycle of B.annulatus was completed experimentally in a period 44 days. The maximum number of eggs produced by one tick was 1420 and minimum 680. The incubation period was dependent on the room temperature and variation in humidity had little influence. The larvae were found to prefer hairy areas of the body for attachment, and they did not attach at the hairless and exposed areas. The engorged females were found to drop off quickly when green grass was spread on the floor of the experimental animal’s shed. The life-cycle patter could be summarised as: Pre-oviposition : 1 to 6 days Oviposition : 5 to 9 days Incubation period : 16 to 30 days Larval fasting : 2 to 5 days Larval feeding : 4 to 7 days Larval moulting : 8th to 12th day of infection Nymphal feeding : 3 to 6 days Moulting of Nymph : 15 to 19th day of infection Adult feeding : 5 to 10 days Detatchment of female : From 20th day of infection onwards Four organophosphorous compounds viz. Malathion, Fenitrothion, Dichlorvos and Phosalone were tested for their action on ticks. The ovicidal and larvicidal actions and the lethal effects on nymphas and engorged females of B. annulatus, R sanguinius and H. bispinosa were studied in the laboratory. Cythion (malathion 50%) 0.5 to 1%, Sumithion (Fenitrothion 50 %) 0.5 to 1%, Dichlorvos (Nuvan) 0.1 to 0.2%, and Zolone (Phosalone 35 %) 0.2 to 0.3% were found effective at different degrees. The efficacy was directly proportional to the concentration of compound and the duration of treatment. Phosalone showed maximum efficacy followed by Dichlorvos. In field trials, all the four compounds gave satisfactory results. But phosalone showed maximum efficacy and least toxicity to animals. Nuvan was also effective but was the most toxic among the four compounds. Thus the acaricide of choice for routine use in Kerala was determined to be Phosalone (35%) at a concentration of 0.3% to be used as a spray or wash.
  • ThesisItemOpen Access
    Experimental studies on rumeno-rectal fistula in calves
    (Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sumangala, M; KAU; Muraleedharan Nair, K N
    Chronic tympany of the rumen is common in cattle. Rumen fistulation has been reported to be successful in the treatment of chronic tympany. Left side external fistulation of rumen has been adopted by many workers. But this was accompanied by seepage of rumen contents and soiling of the flank. While screening the available literature, no reports on an internal rumen fistulation could be found. The effects of rumeno – rectal fistula in experimental calves were studied in the present work. The study was conducted on 12 male calves in two groups , each group consisting of six calves. In the animals of Group – I , laparotomy was performed on the right flank and the right dorsal sac of rumen was anastomosed to the anterior part of the rectum. In the animals of Group II, laparotomy was performed on the left flank and the left dorsal sac of rumen was anastomosed to the anterior part of the rectum. The PH of the rumen contents and the rectal washings, presence of rumen protozoa in the rectal contents, haemoglobin content, packed cell volume, total and differential leucocyte count, nature of dung and the body weight were noted before fistulation and at regular intervals after fistulation. The animals were kept under observation for a period upto 100 days. Closure of the fistula was performed 60 days after fistulation, in two calves, one from each group. The details as described above were noted in these animals also after closure of the fistula. The PH of rectal contents before surgery ranged from 7.3 to 7.5. From the second day after surgery, it ranged between 7.0 and 7.2 in both the groups. The erythrocyte count and haemoglobin content showed a progressive reduction in eight animals and packed cell volume showed a reduction in 10 animals. The variation in the total and differential leukocyte counts were within normal limits. Rumen protozoans were present in the rectal contents from the second day after surgery. Loss of body weight was noticed in all the six animals of Group –I, whereas an increase in body weight was noticed in four animals of Group – II. The feeding habits of the calves were normal. Dung was loose in consistency from the second day after surgery and it contained more fluid soon after drinking water. One animal of Group – I died on the 46th post – operative day, and another one was sacrificed on the 53rd post-operative day. Surgical closure of the fistula was performed in two animals, one from each group. All other animals were sacrificed between 60th and 100th post-operative day. At autopsy omental adhesions were noticed at the site of anastomosis in six calves. The fistula was patent and had a diameter of about 2 cm in 11 animals. Rumen contents were present in the rectum. The site of anastomosis was hard to touch and no other abnormalities could be detected in the rumen and rectum. Complete encapsulation of sutures at the site of anastomosis was noticed in four calves. The healing at the site of anastomosis was complete in all the animals. In the two calves in which closure of the fistula was performed, the healing was uneventful and there was progressive improvement in haemogram values and body weight. It is concluded that functional rumeno – rectal fistula could be established in calves and that it did not cause any untoward clinical manifestations. There was escape of rumen contents through the fistula to the rectum. Surgical closure of the fistula could also be done successfully.
  • ThesisItemOpen Access
    Comparison of serological tests for the detection of leptospira antibodies in immunised animals
    (Department of microbiology, College of veterinary and animal sciences, Mannuthy, 1980) Ravikumaran Nair, R; KAU; Abdulla, P K
    Leptospirosis is a widespread disease of man and animals and is of considerable economic importance besides being a public health problem. The leptospira infection in man and animals may be confirmed either by isolation of the organisms or by detection of specific antibodies in the serum and tissues of infected animals. Isolation of Leptospira is time consuming and beyond the scope of many diagnostic laboratories. In the present study the sensitivity of passive haemagglutination test was compared with the established microscopic agglutination test utilizing rabbit hyperimmune serum as the source of antibody. Leptospira serotypes were grown in Korthof’s medium enriched with 10% haemolysed rabbit serum. By 7 – 10 days satisfactory concentration of the organisms was obtained and was used for MA test. Passive haemagglutination test was carried out employing ethanol extracted antigen from concentrated leptospiral cultures. The PHA test was carried out after determining the optimum dilution of antigen required to sensitize sheep erythrocytes. Hyper immune sera to both serotypes were raised in rabbits by a series of intravenous inoculations. Serum samples for antibody titration was collected at weekly intervals from seven days following the first injection till the 49th day. Antibody titration by MA and PHA tests have shown that all the three animals inoculated with L. autumnalis had a uniform titre of 1:400 on the seventh day whereas the other three animals inoculated with L. pyrogenes showed a low titre of 1:100 by MA test. The PHA titre of both the groups remained the same ie 1:5. The maximum titre of 1:28000 for L. autumnalis was attained on the 21st day and remained unchanged until 35th day. The maximum PHA titre was attained only on 35th day (1:160). The rabbits inoculated with L. pyrogenes showed a maximum titre of 1:3200 by MA and 1:80 by PHA. The results obtained tend to show that PHA titres after reaching the maximum level remained detectable for longer period when compared to MA titres. Erythrocyte sensitizing substance from both the serotypes and the sera samples collected periodically from immunized rabbits were preserved at – 200 C at varying length of time upto three months. There was no deterioration in the stability or potency of ESS or sera on storage.
  • ThesisItemOpen Access
    Investigation on the pathological conditions in the genitalia of female goats
    (Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1980) Ramachandran, K; KAU; Neelakanta Iyer, C P
    A study was undertaken to assess the incidence and nature of pathological conditions affecting the genitalia of female goats. The material for the present study comprised of 950 genitalia, inclusive of 42 gravid ones, collected at random, from the slaughter house, Corporation of Cochin, Ernakulam. The organs which revealed gross lesions and those which were suspected to be affected were subjected to detailed histopathological studies. Pathological lesions of genital organs were observed in 48 (5.053%) genitalia. Thirty two (3.368%) genitalia revealed various pathological changes of the ovaries and pathological changes of the uterus were recorded in20 (2.105%) genitalia. The cervix, vagina and vulva did not reveal any pathological changes in any of the genitalia examined. The following pathological conditions were observed during the course of the present study: Ovarian hypoplasia (0.316%); cystic ovarian degeneration (0.947%); cystic corpus luteum (0.421%); chronic oophoritis (0.211%); multifollicular atresia (0.105%); par ovarian cyst (1.368%); Bursitis (0.842%); salpingitis (0.105%); macerated foetus (1.684%); endometritis (0.211%); cystic glandular hyperplasia (0.105%) and cyst in the endometrium with sub – epithelial haemorrhage (0.105%).
  • ThesisItemOpen Access
    Studies on the incidence , pathogenicity and control of gastro- intestinal nematodes In crossbred calves in Kerala with special reference to strongyloidosis
    (Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Sukumara Pillai, G; KAU; Chandrasekharan, K
    The non – descript and cross bred Calves of Kerala were infected with eight species of gastro – intestinal nematodes viz. Strongyloides papillosus, Neoascaris vitutorum, Cooperia sp., Haemonchus contortus, Bunostomum phlebotomum, Oesophagostomum radiatum, Trichostrongylus colubriformis, and Trichuris globulosa and of which Strongyloides papillosus was the commonest. Incidence of namatodes was more in cross bred calves (64.8 per cent) as compared to non – desctipt calves (51.52 per cent). Out of the cross bred calves, Jersey cross bred calves were found to more susceptible to nematodiasis. Nematodiasis was observed more during the rainy season of the State (June to August). Morphological features of adult worms and infective larvae were redescribed in detail. Strongyloides papillosus produced catarrhal enteritis and frequent diarrhoea in naturally infected cross bred calves. Thiophanate at 100 mg per kg body weight, Mebendazole at 15 mg per kg body weight, Levamisole at 15 mg per kg body weight., Thiabendazole at 100 mg per kg body weight, Tetramisole hydrochloride at 20 mg per kg body weight, Morantel tartrate at 15 mg per kg body weight and parbendazole at 45 mg per kg body weight exerted 100 per cent efficacy against Strongyloides papillosus, whereas, Fenbendazole at 5 mg per kg body weight showed only 83 to 100 per cent efficacy against Strongyloides papillosus, 78 to 100 per cent against ascariasis, 85 to 100 per cent against Strongylosis and 50 to 100 per cent against Trichuriasis.
  • ThesisItemOpen Access
    Pathogenicity of acuaria hamulosa to chicken
    (Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Ganeswara Rao, Y; KAU; Kalyanasundaram, R
    Controlled experiments were conducted with commonly occurring spirurid worm of chicken, Acuaria hamulosa, in two breeds of chicken, namely the White Leghorn (an egg strain) and the White Plymouth Rock (a broiler strain). The blood changes conducted revealed an initial leucocytosis from 14th day to 36th day, followed by heterophilia from 43rd day to 70th day post infection, and a mild eosinophilia throughout the experimental period. The infected White Plymouth Rock birds lost considerable amount of their body weight. In White Leghorn layers, there was great delay in sexual maturity and poor feed efficiency as indicated by reduced egg production. The percentage of establishment in the experiment was found to be not correlated to the infective dose. The pathological changes in the affected gizzards indicated an acute inflammation during the invasive phase of juveniles, followed by a chronic reaction, which by 60th day post infection was well established indicating the development of a host – parasite balances, towards the later stages of infection.
  • ThesisItemOpen Access
    Determination of solids content of milk by specific gravity lactometer
    (Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1980) Babu, Mathew; KAU; Subramaniam, M
    The methods of estimating total solids content of milk having high percentage by Richmond’s formula (TS = 0.25 L + 1.2 F + 0.14) using Quevenne’s specific gravity lactometer, modified Richmond’s formula (TS = 0.25 L + 1.2 F + 0.50) using Zeal specific gravity lactometer, ISI formula (TS = 0.25 DH + 1.22 F + 0.72) and Ling formula (TS = 0.25 DH + 1.21 F + 0.66) using the density hydrometer, along with the percentage of fat estimated by Gerber method were compared with the value obtained by gravimetric method using 307 samples of milk. In all the milk samples analysed the calculated values of total solids by the formula methods were both higher and lower than the gravimetric values. Since the formulae methods did not give a true estimate of the total solids in milk, modifications to the existing formulae were required by applying a correction factor. It was observed that depending up on the percentage of fat in milk samples, different correction factors were needed to be applied to the different formulae for getting the values close to the gravimetric method. Addition of a correction factor of 0.15 and 0.18 was necessary to the Richmond’s formula for milk samples containing 5.1 to 6.0 and 6.1 to 7.0 per cent fat respectively. For the modified Richmond’s formula, addition of 0.57, 0.53, 0.66 and 0.75 was necessary for samples containing 3.0 to 4.0, 4.1 to 5.0 and 5.1 to 6.0 and 6.1 to 7.0 per cent fat. The addition of a correction factor of 0.16 to the ISI formula was essential for samples containing 4.1 to 5.0 milk fat to get the values in agreement with the gravimetric method. No correction factor was necessary, if Ling formula was used. The order of preference for using various formulae will be (1) Ling formula (2) ISI formula (3) Richmond’s formula (4) modified Richmond’s formula. Ling formula can be used without any correction factor for determination of total solids content of milk for varying percentages of fat from 3..0 to 9.0.
  • ThesisItemOpen Access
    Studies on the effects of graded doses of nitrogen , phosphorus and potassium on growth , yield and quality of bhindi
    (Department of Olericulture, College of Horticulture, Vellanikkara, 1980) Subramanian, K V; KAU; Gopalakrishnan, P K
    The yield of bhindi per unit of land and per unit of time has been observed to be very low in our country. A galaxy of reasons- poor genetic potential of the existing genotypes, incidence of parasitic and non- parasitic diseases and above all lack of an appropriate agronomic practices – have been attributed to the poor performance of Indian bhindi. There remains an urgent need to chalk out a fertilizer schedule for the high yielding varieties like Co.I which is popular in Kerala. The present study “studies on the effect of graded doses of nitrogen, phosphorus and potassium on growth, yield and quality of bhindi (Abelmoschus esculentus (L.) Moench.)”, was designed to find out the optimum and economic levels of the three major nutrients (nitrogen, phosphorus and potassium) which would give a yield level above 100 g/ha. A significant increase in yield was observed due to increasing levels of all the three nutrients. In the case of nitrogen the yield was found to increase upto 60 kg/ha and the economic and optimum levels were worked out to be 61 and 61.23 kg/ha respectively. Response to phosphorus and potassium application was observed to be linear. Patterns in uptake of nutrients indicated that a bhindi crop yielding 116.39 quintals of pods per hectare removes 87.81 kg nitrogen, 20.872 kg P2O5 and 103.905 kg K2O/ha. The effects of graded doses of nitrogen, phosphorus and potassium on plant height, leaf production, leaf area index, net assimilation rates, per cent of fruit set and other yield components were also studied.