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ThesisItem Open Access Influence of different levels of energy on growth performance of crossbred pigs(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 2001) Rekha, P; KAU; George, MathenAn experiment was conducted to assess •the influence of different levels of energy on the growth performance of crossbred (Large White Yorkshire x Desi) pigs. Fifteen male (castrated) and fifteen female weaned crossbred piglets with an average body weight of 12.9 kg were divided into three equal groups as uniformly as possible with regard to age, sex and body weight. The three groups of piglets were maintained on three rations with 16 per cent crude protein but differing in their energy content, viz., 2800 kcal (T1), 3000 kcal CT2) and 3200 kcal (TI) of digestible energy (DE) per kg. The average daily gains recorded for the three groups T1, T2 and TI were 262.9, 302.0 and 362.8 g respectively. The cumulative feed conversion efficiencies were 6.0, 5.2 and 4.1 for the groups T1, T2 and TI respectively. The values recorded for TI were higher (P<0.01) than those for T1 and T2. The digestibility coefficients of nutrients except that of crude fibre and crude protein were found to improve with increase in the energy content of the rations. Study of the carcass traits revealed that the body weight at slaughter and dressed weight without head improved as the energy content of the rations increased. However, dressing percentage, carcass length and back fat thickness were not significantly influenced by the energy content of the ration. The cost of feed per kg weight gain of animals was significantly lower (P<0.01) for the dietary treatment T3 compared to that of T2 and Tl, the values being Rs.49.90, 43.30 and 34.10 for Tl, T2 and T3 respectively. The above results indicate that crossbred pigs require 3200 kcal of DE/kg of the ration for better growth performance provided the crude protein level is maintained at 16 per cent.ThesisItem Open Access Trematodes of Paramphistomatidae Infecting Domestic Ruminants(Department of Parasitology, College of Veterinary and Animal Sciences, 1987) Tarun Shankar, Nath; KAU; George Varghese, CThe thesis embodied the results of an investigation on the prevalence of amphistome infection and their specific identity in different domestic ruminants (cattle, buffaloes, sheep and goats) of Kerala state. A total of 1490 faecal samples from domestic ruminants were collected from different places of Kerala State during a period from April 1985 to March 1986. These samples were examined by sedimentation technique to detect infection with amphistomes and their prevalence. Viscera of 780 slaughtered/ dead ruminants from different parts of Kerala were examined in addition and the available amphistomes were collected for the study and specific identification. The flukes were studied alive, flattened and stained and in certain cases by microtomy sections. Result of the study indicated that the prevalence of infection was far more in cattle and buffaloes than in sheep and goats. The rate of prevalence in cattle, buffaloes and goats was 20.16%, 28.57% and 3.22% respectively. In slaughtered/dead animals the prevalence was 33.09%, 34.67%, 4.17% and 5.81% respectively in cattle, buffaloes, sheep and goats. The highest prevalence was recorded during the rainy season and lowest in dry/summer season. Prevalence during south west monsoon was 38.08% and 20.73% during North East monsoon. The seasonal prevalence hardly varied between cattle and buffaloes but it was consistently low in sheep and goats. Most of the prevalence in all animals were of mixed origin. A total number of 17 species belonging to eight genera of amphistomes were identified. Cevlonocotyle spinicephalus (Tandon, 1955) was recorded from new hosts i.e, cattle and goats, cotylophoron chauhani from goats, in addition to the already reported hosts, buffaloes and sheep respectively. The following conclusions are drawn on the basis of the results of this study: Amphistomes are most prevalent in cattle and buffaloes. Though the infection with amphistomes exists throughout the year, it is definitely more in monsoon seasons. Ceylonocotyle spinicephalus (Tandon, 1955) occurs in cattle and goats and cotylophoron chauhani (Gupta and Gupta, 1972 ) in goats also.ThesisItem Open Access Comparative study of genotype environment interactions in sesame(Department of Statistics, College of Veterinary and Animal Sciences, Mannuthy, 1989) Mini, C J; KAU; George, K CThe present study has been conducted to choose a consistent variety for all the regions and all seasons in the light of genotype-environment interaction with the following objectives. (i) to evaluate the existing techniques available for studying GE interaction in sesame (ii) to develop new concepts and methods to solve some problems peculiar to crop sesame like non-linearity of interactions, non-orthogonality of data and different patterns of genotype-environment (GE) interactions that are encountered while studying the stability of varieties simultaneously for several traits.ThesisItem Open Access Investigations on etio-pathology of vomiting in dogs(Department of Clinical Medicine, College of Veterinary and Animal Sciences, Mannuthy, 2001) Muraly, P; KAU; Baby, P GThe study "INVESTIGATIONS ON ETIO-PATHOLOGY OF VOMITING IN DOGS" was conducted in 20 dogs to evaluate ultrasonography and radiography as diagnostic tools in vomiting dogs; to assess hydration status, electrolyte and acid-base balance in vomiting dogs and to correlate clinico-pathologic findings with radiographic and ultrasonographic changes. Various parameters such as history, physical examination, hydration status, ultrasonography, radiography-plain and contrast, haematology, serum biochemistry, and wherever possible histopathology were studied. Most of the dogs under study had bile stained watery vomitus but dogs with pyloric stenosis had frothy or watery white vomitus. The frequency of vomiting in dogs with gastritis and gastrointestinal (GI) obstruction was two to seven times per day, it was variable in dogs with hepatic and renal disorders, but was associated with food intake in dogs with pyloric stenosis. Physical examination was found useful in dogs with GI obstruction, while it was non-specific in dogs with gastritis and renal disorders. Capillary refill time (CRT) and degree of sunken eye balls were helpful to assess dehydration. Estimation of volume of packed red cells (VPRC) was found beneficial to assess dehydration unless the dogs are anemic. Ultrasonography could not identify any lesions in dogs with gastritis, but was useful to detect GI obstructions due to pyloric stenosis, intussusception and foreign body and to characterise lesions in the parenchymal organs like liver and kidney. While plain radiographs could give indication to possible non-radiopaque GI obstructions, contrast radiography was required to confirm. Radiography could not identify any lesions in dogs with gastritis, hepatic and chronic intestitial nephritis. Hypokalemia with metabolic alkalosis was the significant electrolyte and acid-base derangement in dogs with vomiting due to gastritis and GI obstructions.ThesisItem Open Access Immunodiagnosis of bovine gastrothylacosis using coproantigens(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 2000) Kandasamy, A; KAU; Devada, KA study was conducted on the prevalence of paramphistomatidosis in Thrissur from June 1999 to May 2000, feasibility of coproantigen detection by ELlSA and comparison of sensitivity of ELlSA using coproantigens and ELlSA using serum antibodies in diagnosis of gastrothylacosis, caused by Gastrothy/ax crumenifer, in cattle. It was noted from the registers maintained at the University Veterinary Hospitals at Kokkalai and Mannuthy and that at the Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, that out of a total number of 1534 faecal samples from bovines examined, 253 (16.5 per cent) animals were found to be positive for amphistome eggs with the maximum prevalence (23 per cent) in June and July. Generally the infection was prevalent throughout the year. An indirect ELlSA using rabbit hyperimmune serum against somatic antigens of G. crumenifer was performed to detect coproantigens in faecal samples collected from 100 known G. crumenifer infected cattle. Seventy four samples were found to contain detectable levels of coproantigen indicating a sensitivity of 74 per cent. Serum samples collected from the same infected cattle were tested for antibodies to G. crumenifer by an indirect ELlSA using somatic antigens. Fifty one samples were found positive for antibodies indicating a sensitivity of 51 per cent. It was seen that when 43 cattle were positive for both coproantigens and serum antibodies, 18 cattle were negative for both of them. Although 31 cattle which were negative for serum antibodies were found positive for coproantigens, eight cattle negative for coproantigens were found positive for serum antibodies. The results showed that coproantigen detection, which revealed a higher sensitivity than the detection of serum antibodies by ELlSA, is feasible for the diagnosis of gastrothylacosis in bovines.ThesisItem Open Access Effect of processing and freezing procedures on the acrosome morphology of buck spermatozoa(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1998) Ranjini, A; KAU; Prabhakaran Nair, KSix pooled semen samples (two ejaculates) of good quality from five Malabari crossbred bucks were processed and frozen in two different protocols to evaluate the effect of processing and freezing procedures on the acrosome morphology of buck spermatozoa. In protocol I, the samples were diluted 10 fold in Tris buffer before centrifuging twice and the final pellet was re-suspended in the non glycerolated fraction of Tris yolk diluent. The sample was glycerolated (six per cent), equilibrated (four hours), frozen (eight minutes), and thawed (250 C for 30 seconds). In protocol 11, centrifugation was done only once, after 15 fold dilution in Tris buffer. The re suspended pellet was glycerolated (seven per cent), equilibrated (three hours), frozen (10 minutes) and thawed (60° C for 10 seconds). The semen characters such as motility, live sperm, sperm abnormalities and acrosome abnormalities were evaluated at the end of washing and initial extension (stage I), cooling to 5° C (stage II), glycerolisation and equilibration (stage Ill) and freezing and thawing (stage IV). The results were compiled to evaluate the effect of different processing and freezing procedures on the semen characters in general and acrosome morphology in particular. The semen sample used for split sample dilution had a mean volume of 1.3282± 0.067 ml, creamy in colour, DDDD density, ++++ mass activity, pH of 7.275 2± 0.040 and a concentration of 2972 2± 293 millions per ml. No significant difference in the above semen characters were found between bucks. The initial sperm motility of 82.000 2± 0.606 was found to drop significantly during processing and freezing and the final post thaw motility obtained was 44.000 2± 0.790 in protocol I. Similarly in protocol II the initial motility dropped from 81.375 2± 1.089 to 44.750 2± 1.075 at the end of stage IV. Even though there was significant drop in motility between stages in both the protocols, there was no significant difference in the corresponding stages of the two protocols. It could be inferred that good post thaw motility was obtained in both the protocols. The fact that a single washing and centrifugation was only adopted in protocol II makes it a more acceptable procedure for buck semen freezing. The mean live sperm percentage of fresh semen was evaluated using both NE and NEG staining technique. The percentage of live sperms of 90.050 2± 0.801 was found to decrease to 54.250 2± 0.593 after freezing and thawing in protocol by NE staining. Similarly in protocol 11, the mean percentage of live sperms was found to reduce to 53.125 2± 0.793 with the same staining. Even though there was significant difference in the live sperm percentage between stages within protocol I and II no significant difference in the live sperm percentage between the corresponding stages of protocol I and I I . With NEG staining the initial live sperm percentage of 80.850 ± 1.494 was found to drop to 54.875 ± 0.677 in protocol I as against 53.400 ± 0.730 in protocol II. While there was significant difference in the live sperm percentage between stages within protocol I and II there was no variation between corresponding stages of the two protocols. A significantly lower percentage of live sperms was recorded with NEG staining when compared with NE staining probably on account of the fact that the differentiation of live and dead sperm was difficult in the former staining method as live sperms were stained light blue instead of colourless. The mean percentage of abnormal sperms of 3.050 ± 0.245 in fresh semen did not register any significant increase during processing. However, there was significant increase in the percentage of sperm abnormalities during freezing and thawing with the final abnormality percentage of 7.125± 0.706 in protocol I and 6.300± 0.36 in protocol II. The initial acrosomal abnormality of 8.825 in the fresh semen steadily rose to 23.375 in protocol I as against 19.825 in protocol II at the end of stage IV. There was no significant difference in the percentage of various acrosomal abnormalities between corresponding stages of the two protocols. However, there was significant increase in the acrosomal abnormalities during glycerolisation, equilibration, freezing and thawing under both the protocols. It was concluded that the processing and freezing under two different protocols did not significantly alter the post thaw motility, percentage abnormal and dead sperms and acrosomal abnormalities. A good post thaw motility and low acrosomal abnormality was obtained with a single washing of buck semen with 15 fold Tris buffer which was comparable with double washing with 10 fold Tris buffer.ThesisItem Open Access Effect of probiotic supplementation on the performance of broiler chicken(Department of Poultry Science, College of Veterinary and Animal Sciences, Mannuthy, 2001) Sabitha Mahaboob Kadari, A; KAU; Elizabeth, V KThe effects of different levels of pro biotic (Lactobacillus acidophilus, Streptococcus faecium and Yeasacc 1026) supplementation• at 0.025 and 0.05 per cent of the ration on the performance of broiler chicken were evaluated using 144, one-day old, commercial broiler chicks for a period of eight weeks. The birds were divided into three dietary treatment groups viz., standard broiler ration (T 1), standard broiler ration with 0.025 per cent probiotic (T 2) and standard broiler ration with 0.05 per cent probiotic (T3). Standard broiler ration was formulated as per Bureau of Indian Standards (1992) specification for broiler chicken feed. The 0.025 per cent probiotic supplemented birds showed a significantly higher (P<0.05) body weight upto six weeks of age. At the end of eight weeks of age, the 0.05 per cent probiotic fed birds grew faster. The body weight gain was significantly higher in 0.025 per cent probiotic supplemented group upto six weeks of age but was statistically non-significant upto eight weeks of age. The feed intake was not statistically significant throughout the experimental period. Eventhough the feed efficiency was significantly (P<0.01) better in the group fed with 0.025 per cent probiotic at the end of second week, it was statistically non-significant at sixth and eighth weeks of age. The protein efficiency was not significantly different throughout the experimental period. The serum cholesterol levels were significantly (P<0.01) reduced in both the probiotic supplemented groups. The serum protein level was not affected by probiotic supplementation. The processing Yields did not show any significant difference among treatments. The mortality percentage was not affected by treatments. Cost of production of broilers in the 0.025 per cent probiotic group was lower when compared with other two groups at the end of six weeks of age, while it was lower in the 0.05 per cent probiotic supplemented group at the end of eight weeks of age. It can be concluded that probiotic supplementation in standard broiler ration at a lower level was beneficial in the early stages of growth.ThesisItem Open Access Preparation of mozzarella cheese using skim milk filled with coconut milk(Department of Dairy Science, College of Veterinary and Animal Sciences, Mannuthy, 1994) Gnana Selva, Johnson; KAU; Mukundan, MA detailed study was carried out to determine the quality of coconut fat filled milk for the preparation of Mozzarella cheese and why drinks. Literatures based on filled milk products has been reviewed, apart from the preparation of cheese and why drinks. The control samples of Mozzarella cheese and whey drinks were prepared using cow’s milk. Experiment I products were prepared from milk in which 50 per cent of milk fat was replaced with coconut fat. Experiment II products were prepared from cheese milk in which 100 per cent of milk fat was replaced with coconut fat. All the samples of milk were standardized to 4 per cent fat. A total of 6 trials were carried out to obtain reliable data for statistical analysis. The acidity, pH, stretchbility and FDM content were found to be similar in control, experiment I and II Mozzarella cheese. Eventhough, the control Mozzarella cheese were found to have slightly higher yield protein, fat and lower moisture content, the experimental I and II. Mozzarella cheese also satisfied the requirements for good quality Mozzarella cheese. The control Mozzarella cheese got maximum score on sensory evaluation than the experiment I and II Mozzarella cheese. Pineapple and Lemon falvoured control, experiment I and II whey drinks were found to be equally acceptable with nodifference on storage studies ar 5 + loC. Total bacterial count on whey drinks were also made. The studies revealed that the cow milk in which the milk fat replaced to the extend of 50 per cent and 100 per cent with coconut fat can be effectively utilised for preparation of Mozzarella cheese. The quality of such cheese is comparable with that made from cow milk.ThesisItem Open Access Metabolic profile of downer cow syndrome(Department of Clinical Medicine, College of Veterinary and Animal Sciences,Mannuthy, 1994) Mhachuvino Catherine, Khatsu; KAU; Alikutty, K MThe metabolic profile of ‘Downer Cow’ syndrome in field conditions was studied. Fourteen field cases of ‘Downers’ in crossbred dairy cows aged three to thirteen years, ranging from 250 to 300 kg body weight from Trichur district were selected at random and utilized for the study. Fourteen apparently healthy crossbred dairy cows of similar age group and body weight, maintained under similar conditions of feeding and management from the area from which the clinical cases studied were also selected at random and utilized as the healthy controls. Samples of blood for haematological and biochemical parameters, urine and dung from both healthy and diseased animals were collected and analysed using standard methods. Analyses of the data from fourteen diseased animals indicated a higher incidence in Jersey crossbred cows during summer season. Prominent clinical signs were sternal recumbency exhibiting hindquarter weakness and reduced feed and water intake. However, the affected animals remained bright and alert with no evidence of any systemic disturbances. The clinical data were within physiological limit. Highly significant increase in PCV and significant increase in Hb but no significant difference in ESR, RBC and WBC were observed. Lymphopenia, neutrophilia and eosinopenia were observed with no variation in basophils and monocytes counts. Biochemically, hypocalcaemia,hypophosphataemia, hypoproteinaemia and hypoalbuminaemia were obtained from ‘Downers’ with no significant variation in blood glucose, urea nitrogen, sodium, potassium, magnesium and albumin/globulin ratio. Urinalysis revealed no consistent result indicative of any systemic involvement and no parasitism on dung examination microscopically.
