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Open Access
Biotic agents for the management of American serpentine leaf miner, Liriomyza trifolii(Burgess) (Diptera:Agromyzidae)
(Department of agricultural entomology, College of horticulture, Vellanikkara, 2014) Jyothi Sara, Jacob; KAU; Maicykutty P, Mathew
A study on “Biotic agents for the management of American serpentine leaf miner, Liriomyza trifolii (Burgess) (Diptera: Agromyzidae)” was carried out at the Department of Agricultural Entomology, College of Horticulture, K.A.U., Vellanikkara during 2011-2013 with the objectives of collection and identification of indigenous natural enemies and to assess the pathogenicity of the entomopathogens to explore the feasibility of utilizing them for its management. Surveys were conducted in the vegetable fields for the collection and identification of natural enemies associated with L. trifolii in three districts, namely, Thrissur, Ernakulam and Kottayam from January to March, 2011. The surveys revealed the occurrence of nine species of hymenopteran parasitoids. The per cent parasitism varied from 10.96 to 58.99 per cent among the crops surveyed. Three species of eulophids, namely, Cirrospilus acadius Narendran, C. brevicorpus Shafee & Rizvi and Aprostocetus sp. as well as the braconid, Toxares sp. are new reports for India. Among the parasitoids, Closterocerus spp. were the dominant group followed by Chrysonotomyia sp. All parasitoids were solitary, larval endoparasitoids except Toxares sp. which was larval-pupal in nature. One species each of small ants (Formicidae) and a dipteran fly (Dolichopodidae) were observed as predators on L. trifolii. In the study, no entomopathogens were observed from L. trifolii. Considering the level of pesticide consumption in vegetable crops that undermine the potential of insect parasitoids and also that no entomopathogens could be observed during the survey, it was decided to evaluate entomopathogenic nematodes (EPNs) as biocontrol agents against L. trifolii. Isolation of EPNs from 72 soil samples from Thrissur, Ernakulam and Kottayam districts yielded four isolates of Steinernema carpocapsae. Bioefficacy studies carried out on these four isolates along with Steinernema bicornutum and Heterorhabditis indica showed that S. carpocapsae Isolate - 1 had the lowest LC 50 , LC 90 and LT values indicating their higher effectiveness against the maggots of the pest. 50 Pot culture study conducted to compare the potential of S. carpocapsae Isolate - 1 with other treatments showed that azadirachtin 1 EC at 0.005% was the most effective causing 84.51 per cent mortality to the maggots of L. trifolii. This was followed by the foliar application of H. indica at 32 infective juveniles (IJs)/ maggot which caused 18.98 per cent mortality. Application of Beauveria bassiana at 1×10 7 spores/ ml was not effective. In the field evaluation, fipronil 5 SC at 0.002% was found to be the most effective treatment for controlling L. trifolii followed by azadirachtin 1 EC at 0.005%. Compatibility of the IJs of the S. carpocapsae Isolate - 1, S. bicornutum and H. indica was studied with ten commonly used insecticides in the laboratory by direct exposure method. Chlorantraniliprole 18.5 SC at 0.005% was found to be the most compatible insecticide with S. carpocapsae isolate - 1 causing only 0.17 per cent mortality to IJs at 72 hours after treatment (HAT). Quinalphos 25 EC at 0.05% and chlorpyriphos20 EC at 0.05% were highly incompatible, causing 96.17 and 92.87 per cent mortality of the nematodes. Dimethoate 30 EC at 0.04% was the most compatible insecticide with S. bicornutum and caused only 0.60 per cent mortality at 72 HAT and was followed by azadirachtin 1 EC at 0.005% with 0.78 per cent mortality to the IJs. Quinalphos 25 EC at 0.05% caused 99.93 per cent mortality at 72 HAT. Heterorhabditis indica was compatible with all insecticides except quinalphos 25 EC at 0.05% which was moderately toxic resulting in 39.6 per cent mortality. The virulence, pathogenicity and multiplication of the survived IJs were not affected by the insecticide treatments. Parasitoids and EPNs were observed as potential candidates for the management of L. trifolii. Hence future studies on the bio-ecology and mass production of dominant parasitoids and standardization of methods to improve the efficacy of EPNs are suggested for the successful control of L. trifolii in polyhouses as well as in the field.
Open Access
Participatory action research for renumerative rice production
(Department of Agricultural extension, College of Horticulture, Vellanikkara, 2004) Parvathy, S; KAU; Ahamed, P
As with the Complex, Diverse and Risk- prone (CDR) rice systems of many Asian countries, the Kerala state of peninsular India suffers from the fast depleting paddies and the ' high cost- low remuneration syndrome'. Crucial rice technologies have been blamed by stakeholders for the insensitivity to micro farming situations A multidisciplinary stakeholder Participatory Action Research (PAR) of emancipatory type and collaborative mode was done for three years (2001-2004) on identification and prioritization of constraints to profitable rice production to explore the available cost-reducing and productivity increasing technological options. The project focussed on screening of technological modules through farmer participatory field assessment and arrived at locally adaptable and remunerative technology packages. The project also developed and standardised an extrapolatable stakeholder participatory assessment model and protocol. The programme had a blend of extension approaches, research designs and tools like "ex-post facto", benchmark appraisal through Participatory Learning and Action (PLA), exploratory, diagnostic, evaluative, field experiments and analytical studies. The PAR was done in two rice ecosystems (irrigated and rainfe:l) of the midland, laterite belt of Kerala state, India. Fourteen technology modules were fitted into the PAR, under .. , each of the three treatments, viz., farmers' practice, recommended packages of the formal research system and location specific I technology components" jointly decided by the research team extensionists and farmers. Each technology component was subjected to five types of analysis viz., agronomic, statistical, economic, farmers perceptions and reactions, post-trial follow up analysis of adoption in the succeeding cropping seasons. The participatory interventions significantly influenced the level of technical knowledge and extent of adoption of adaptable technology modules, typifying the cognitive impact of an emancipating action research. The short duration red rice varieties "Kanchana" (Ptb 50) and "Kairali" (Ptb 49) proved to be the best first crop and second crop varieties respectively, to replace the ruling cultivars. The technology modules recommended by the formal research system viz., seed treatment with fungicides for the first crop season and Pseudomonas fluorescence for the second crop season were adaptable technologies. The PAR came out with an efficient planting density and crop geometry package (line transplanting; 15 x 10 cm; 67 . , hills/m'; 2-3 seedlings/hill) to replace the conventional planting system. The existing formal recommendations including IPM and INM practices could enhance and combat weeds, pest and diseases thereby enhancing crop yield. Harvesting with self- propelled reaper and threshing with mechanised thresher were cost effective, drudgery alleviating and time saving. The net result of the action research was a set of adaptable technological package for remunerative rice production in the CDR rice production systems. Cognitive and behavioural impact on the participants; and the standard methodology and protocol for participatory technology validation for rice in particular, and for any farm enterprise in general, with extrapolative effect.
Open Access
Bioactivity of carotenoids from shrimp shell waste
(Department of Processing Technology,College of Fisheries,Panangad, 2010) Sindhu, S; KAU; Sherief, P M
Shrimp processing waste is the single largest industrial waste in the country causing diverse environmental problems. A study was carried out to assess the extractability of astaxanthin from shrimp waste in different organic solvents and vegetable oils. Extraction was tried using wet and dried waste, with and without deproteinisation. Waste was subjected to deproteinisation using alkali and enzyme (pancreatin). The different solvent systems tried were ether:acetone:water (15:75:10 v/v/v), acetone, hexane:isopropanol (3:2 v/v) and 90% acetone v/v. Astaxanthin in the extract was quantified by measuring the OD at 470 nm in hexane. Extraction was also done using vegetable oils viz. coconut oil, soybean oil and sunflower oil. Quantification of astaxanthin in pigmented oil was done by measuring the absorbance at 485 nm using 2155 as extinction coefficient. Astaxanthin yields from deproteinised samples were significantly lower than those from non deproteinised samples. The highest astaxanthin yield of 87.14 ± 4.55μg/g was obtained with non deproteinised wet waste extracted using acetone. The astaxanthin yield was significantly lower when oil was used as the extraction medium. Of the three oils coconut oil gave the highest yield. The results showed that acetone is the best solvent for extracting astaxanthin from shrimp shell waste in wet condition. The astaxanthin content in Aristeus alcocki shell waste is double that of Pandalus borealis shell waste, which is currently used as the commercial source of astaxanthin. The deep sea species Aristeus alcocki can thus be considered as a better source of astaxanthin for commercial exploitation than Pandalus borealis. TLC analysis of the shell waste extract showed that it contains free astaxanthin, astaxanthin monoester and astaxanthin diester in the ratio 1:1:2. GLC identification of the fatty acids esterified with astaxanthin revealed that saturated fatty acids, MUFA and PUFA are in the ratio 5:3:2 in monoester, whereas in diester they are in the ratio 4:3:3. The main fatty acids in monoester and diesters are palmitic acid, oleic acid, stearic acid and PUFAs: DHA and EPA. The in vitro antioxidant activity of the astaxanthin extract showed significant hydroxyl radical scavenging activity, superoxide anion scavenging activity and inhibition of lipid peroxidation. The IC50 values obtained were 56.43 ± 1.06 ng/ml, 27.91 ± 0.54 ng/ml and 26.54 ± 0.42 ng/ml, respectively. The antioxidant activity of astaxanthin from Aristeus alcocki was obtained at nanogram levels. This powerful antioxidant function may be due to the unique molecular structure of astaxanthin and synergistic effect of astaxanthin and PUFAs present in the astaxanthin monoester and diester fractions. The astaxanthin extract from shrimp shell waste significantly reduced carageenan induced paw edema in mice, percentage inhibition being 47.83 and 67.11 percent at astaxanthin concentrations of 0.5 mg/kg body weight and 1.0 mg/kg body weight, respectively. The inhibition of inflammation at 1.0mg/kg body weight was greater than that produced by the standard reference drug diclofenac. Cardioprotective effect of astaxanthin was examined in isoproterenol induced myocardial infarction in rats. Levels of diagnostic marker enzymes, LDH, CPK, GOT, GPT, CK, CK-MB in plasma, lipid peroxides, ascorbic acid, reduced glutathione and the activities of glutathione-dependent antioxidant enzymes GPx, GR, GST and antiperoxidate enzymes CAT, SOD and the membrane bound enzyme Na+ - K+ ATPase in the heart tissues of experimental groups of rats were determined. The prior administration of astaxanthin @ 10mg/kg feed for 45 days significantly prevented the isoproterenol-induced elevation in the levels of diagnostic marker enzymes in plasma, induction of lipid peroxidation and alterations in the level of reduced glutathione and in the activities of glutathione dependent antioxidant enzymes and antiperoxidative enzymes of experimental rats. Feeding astaxanthin caused a decrease in the inhibition of Na+ - K+ ATPase activity against isoproterenol induced myocardial infarction. The powerful cardioprotective effect of astaxanthin can be attributed to the multiple independent mechanisms viz. antioxidant effects, singlet oxygen quenching ability and inhibition of lipid peroxidation of membranes, increased functional gap junctional intercellular communication, anti-inflammatory effects etc. Immunostimulatory action of astaxanthin extract was evaluated in experimental mice. Astaxanthin administration was found to enhance the proliferation of spleen cells and bone marrow cells. Esterase activity was found to be enhanced in bone marrow cells indicating increased maturation of cells of lymophoid linkage. Astaxanthin also enhanced number of antibody forming cells and circulating antibody titre. Thus astaxanthin exhibits strong immunomodulating properties. A significant reduction in the viability of ascites tumour cells DLA in vitro was noted in the current study. The % viability was reduced to 4.34 % at a concentration of 15μg astaxanthin/ml. The cytotoxic action of astaxanthin against DLA may be through induction of apoptosis or through a different pathway. Antitumour activity of astaxanthin was studied by ascite and solid tumour models in mice. An increase in life span of about 67 % was noted in DLA bearing mice administered with astaxanthin at 5 mg/kg body weight. The tumour volume and tumour weight were significantly lower in mice injected with 5 mg/kg body weight astaxanthin. In vitro studies revealed that astaxanthin from shrimp shell waste of Aristeus alcocki inhibited the proliferation of cervical cancer cells HeLa in a dose dependent manner.
Open Access
Genetic variability and plus tree selection in natural populations of Malaveppu (Melia dubia Cav.)
(Department of Forest biology and tree improvement, Vellanikkara, 2019) Binu Kamalolbhavan, N; KAU; Santhoshkumar, A V
Melia dubia commonly known as Malabar neem is a fast growing indigenous species. It is considered as a major wood in plywood and paper industries and the plant is put into various medicinal uses, preferred for use in packing cases, cigar planks etc. There are no identified genotypes of melia, suitable for Kerala. This is one of the reasons which has made a dearth in the availability of good planting material. Hence, a study was conducted to determine the genetic diversity of the trees and select plus trees from the forests of Kerala. Fifteen locations spread over eighteen localities where the species is common were identified from secondary data and reconnaissance survey. In total 281 trees were selected for the survey. The important quantitative and qualitative characters of the trees were determined. Based on baseline selection system, a regression analysis was done between the (DBH)2 x height and (crown width)2 x crown length separately for each location and the trees above the regression line having highest score for the qualitative characters were selected. This was to ensure that a minimum of one tree got selected from a locality to ensure diversity in selection. The study showed that variability existed for all the qualitative characters and it was more within the localities when compared to between localities. In total twenty five trees were selected. Clustering done for the twenty five plus showed that Melia dubia genetic resources resolved the trees into seven clusters and it was found that grouping were independent to the geographic locations. Inter and intra cluster divergence were also studied. The evaluation of the seedling progenies from the twenty five plus trees were done in the nursery for five months. Significant differences among the progenies of the selected trees were observed for various morphological and physiological characters studied. The performance of the progenies of two trees from Tholpetty (FCV-MD-03 and FCV-MD-04) were the best in most of the parameters studied. The genetic analysis of the causes of variation for the morphological and physiological traits were studied. The values for the phenotypic coefficient of variations ranged from 12.62 per cent for height to 24.53 per cent for biovolume. ii The result indicated that the values for genotypic coefficient of variation was less than the phenotypic coefficient of variations for all the traits studied, indicating existence of environmental effect on these characters. Heritability estimates in broad sense were observed higher than 50 per cent for the quantitative characters such as height, collar diameter, number of leaves, AGR and biovolume. Heritability was also higher for all the physiological characters studied. The genetic gain was also high indicating possibilities of using selections for further breeding. The correlation studies on the morphological and physiological characters showed that the height was positively correlated with all the morphological and physiological characters studied except leaf temperature. Photosynthetic rate was positively correlated with the height, chlorophyll and relative water content of the leaves. For standardization of clonal progenies, cuttings from the seedlings, semi hardwood cuttings from mature trees and root suckers by damaging the roots were taken to develop a method for mass multiplication of the Melia dubia. Three different auxins IBA, NAA, IAA at different concentrations were administered. The result showed that the best treatment for the cuttings and rootsuckers were IBA at 1000 ppm. For the semihardwood cuttings, miniclonal technology was used and the best treatment observed was IBA at 6000 ppm.
Open Access
Characterization and identification of black pepper accessions (Piper nigrum L.) for stress tolerance and quality
(Department of plant breeding, College of Horticulture, Vellanikkara, 2019) Prakash, K M; KAU; Jiji, Joseph
Black pepper (Piper nigrum L.), often described as the ‘King of spices’ is the most important spice crop, grown for its berries in the world. Indian pepper is preferred across the globe due to its intrinsic qualities. Foot rot is a devastating disease of black pepper. In the changing climate, drought can be a major threat in black pepper production. Hence, the present study was taken up at College of Horticulture, Vellanikkara and ICAR-IISR, Kozhikode to characterise and to identify superior accessions of black pepper for yield, quality and tolerance to biotic and abiotic stresses. Fifty accessions of black pepper in the bearing stage maintained in the National Active Germplasm Site of ICAR-IISR, Kozhikode formed the base material for the study. The accessions were characterised for fifty qualitative and fifty quantitative characters following the descriptor developed by IPGRI (1995). Wide variability was observed among the accessions for ten qualitative characters. Quantitative characters of shoot, leaf, spike and fruit also showed wide variability. Field tolerance to foot rot disease and pollu beetle infestation was observed among the accessions. Twenty accessions were selected from the base collection based on superiority of yield (> 450g green berries/vine) , field tolerance to foot rot disease infection (biotic susceptibility score 1) and pollu beetle infestation (biotic susceptibility score 1-3). They were further evaluated for biochemical principles of quality, tolerance to foot rot disease under artificial inoculation and tolerance to drought by physiological and biochemical analyses. Piperine, essential oil and oleoresin ranged from 3.61 - 6.96 per cent, 3.00 - 5.87 per cent and 7.10 - 11.18 per cent, respectively, across the accessions. The accessions with high value of piperine, essential oil and oleoresin were identified as 7293, 7211 and 7289 respectively. The two accessions identified viz. 7293 and 7252 contained more piperine than the highest of Panniyur 2 (6.6 per cent) reported among the released varieties . Artificial inoculation of selected accessions using Phytophthora capsici culture for screening for foot rot disease resistance based on over all disease severity index of both stem and leaf lesions showed that accession 7259 was moderately resistant. The selected accessions did not exhibit significant variation for various physiological and biochemical parameters at field capacity. However higher value of photosynthesis, chlorophyll content, chlorophyll stability index, relative water content and membrane stability index and low leaf temperature were observed for accessions viz. 7215, 7240, P 5 and 7241 after five days and ten days of moisture stress induction following field capacity compared to other accessions. Higher values of proline, SOD, catalase and peroxidase were also observed for these accessions. The visual scoring showed that accessions with higher values for most of physiological and biochemical parameters of drought tolerance viz. 7215, 7240, P5, and 7241 had lesser number of fallen leaves and more number of leaves retained at permanent wilting point (PWP). The accessions 7215 and 7240 took twenty days to reach PWP compared to eleven accessions which took only 16 days to reach PWP. Foliar nutrition with sulphate of potash, IISR - Power mix and Pink Pigmented Facultative Methylotrophs (PPFM) had positive effect on drought tolerance for the accessions (7215, 7240, P5 and 7241) having natural tolerance. The identified accessions with high yield , quality and tolerance to biotic or abiotic stress can be used for further breeding programme.
Open Access
Genetic characterization, controlled breeding and development of transgenic varieties of puntius denisonii (day, 1865).
(Department of Aquaculture, College of Fisheries, Panangad, 2010) Manoj, C K; KAU; Mohanakumaran Nair, C
Puntius denisonii, a beautiful ornamental fish indigenous to the Western Ghats, which has been indiscriminately exploited from the different rivers of Kerala has been recently declared to be vulnerable by the IUCN. The population structure and genetic diversity of P. denisonii has not yet been studied and documented. Many previous attempts to breed this fish in captivity have yielded negative results. The increasing demand for this fish to decorate aquariums worldwide could be satisfied only by developing controlled breeding techniques and larval rearing of its fry. In the present study, the present population structure of P. denisonii has been studied combining both phenotypic and genotypic techniques. Fishes were collected from Irrity, Chaliyar and Periyar rivers of Kerala. Truss network analysis was conducted and the size adjusted morphometric variables were subjected to Principal Component Analysis and Canonical Variance Analysis. Scatter diagram and Dendrogram was plotted using PCA and CVA loadings. The Irrity and the Chaliyar populations were grouped on the positive sector of the PC and CV component showing morphological similarities between the two populations while the Periyar population was placed in the negative sector of the component separated far from the other two. The PC scores were used to find out the variables showing maximum variation between fishes collected from different rivers. RAPD PCR was conducted after isolating DNA from the fins of different populations of P. denisonii. Universal random primers were screened and the primers that produced reproducible bands were selected. Popgene analysis of the binary data yielded the genetic structure of different populations of P. denisonii. Number and percentage of polymorphic loci, Nei's (1973) gene diversity, Shannon's Information index Lewontin (1972), Nei's Unbiased Measures of Genetic Identity and Genetic distance and Dendrogram Based Nei's (1978) Genetic distance using UPGMA --Modified from NEIGHBOR procedure of PHYLIP Version 3.5 were studied. The results obtained supports the truss analysis in that the Irrity and Chaliyar populations in Northern Kerala are genetically more similar while that of Periyar population in Central Kerala are distinct. P. denisonii was successfully induced bred under controlled conditions with synthetic hormone preparations Ovaprim and WOVA-FH. Stress during transport and handling was minimized and live feed was supplemented to enhance maturation of the broodstock. The whole developmental sequence starting from fertilized eggs to hatching was photographed and documented. It took 29-30 hours for the eggs to hatch at 280C. Rearing of fry was successfully accomplished under laboratory conditions. In an attempt to develop transgenic varieties of P. denisonii, pCMV-GFP was electroporated into newly fertilized eggs, maintained in hypoosmolar electroporation buffer. The electroporation parameters that yielded best results were 20V, 3 bursts at 1 second interval. Fin clips were taken from the transgenic individuals reared for a period of 6 weeks. Dot blot test was positive showing integration of the GFP gene in P. denisonii, eventhough expression was not detected under blue or UV light. The genetic and phenotypic data of P. denisonii populations in the present study will aid as a base line for formulating conservation procedures to protect the genetic diversity of wild ones. Stock identification studies are recommended for more concise information on each population. Moreover, the larval rearing and controlled breeding techniques along with the genetic diversity studies will help to design captive breeding programs and enhance the production of hatchery bred ones to meet increasing demand. Further research is recommended for generating transgenic lines with uniform GFP expression.
Open Access
Enhancement of propagation efficiency in exotic varities of heliconia
(Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2008) Reshmi, C R; KAU; Sheela, V L
Heliconias are among the most popular garden plants, both for the ease with which they can be grown and the sheer magnificence of the blooms. Studies were conducted to standardize rapid propagation techniques under in vitro and in vivo conditions. Three heliconia varieties belonging to three distinct groups viz., St. Vincent Red (small erect type), Golden Torch Adrian (hybrid) and Sexy Pink (large pendent type) were selected for the study. For in vitro culture establishment, shoot tip explants were effective. The treatment of shoot tips with 0.10 per cent mercuric chloride for ten minutes followed by dipping in 0.05 per cent mercuric chloride for five minutes (after trimming) resulted in better surface sterilization. Longitudinal cutting of the in vitro established shoot tip with apical dome into two halves and culturing them separately produced the highest number of axillary buds. Addition of 0.05 per cent activated charcoal to the establishment media reduced the media browning and hastened shoot initiation. For culture establishment of all the three varieties, MS medium supplemented with BA 5.00 mg l-1 was found to be the best. Murashige and Skoog medium supplemented with BA 2.00 mg l-1 resulted in the highest shoot proliferation in the variety St. Vincent Red. In Golden Torch Adrian, BA 2.00 mg l-1 + NAA 0.20 mg l-1 gave better results. In the variety Sexy Pink, Kinetin 5.00 mg l-1 + NAA 0.20 mg l-1 was the best. For further multiplication in Sexy Pink, BA 1.00 mg l-1 was sufficient. Solid culture medium was better for shoot proliferation in the variety Sexy Pink. Higher sucrose concentration (40.00 g l-1) increased the multiplication rate, but reduced the length of shoots as well as the number of leaves. Addition of activated charcoal to the media as well as exposure of cultures to light had negative effect on shoot proliferation. Full MS medium was found to be the best for in vitro rooting of the variety Sexy Pink. Addition of NAA 0.50 mg l-1 to the MS medium gave better results for in vitro rooting in Golden Torch Adrian and Sexy Pink. Sucrose @ 30.00 g l-1 was sufficient for in vitro rooting in the variety Sexy Pink. Activated charcoal delayed root initiation and reduced the number of roots in the variety Sexy Pink. Sand recorded 90.00 per cent survival in all the three varieties after two months of planting out. At varietal level, significant difference was evident in the total number of suckers. In the first experiment, the variety St. Vincent Red (3.82) was significantly superior in terms of total number of suckers. However, in the second experiment, St. Vincent Red (4.06) was on par with Golden Torch Adrian (4.10). The variety Sexy Pink produced comparatively taller suckers in both the trials. Varietal variation in the number of leaves was observed only in the earlier stages. During the preliminary field experiment, the variety Sexy Pink excelled in leaf area at almost all stages of observation. Among the three varieties, highest collar girth was recorded by suckers of Sexy Pink variety during the first two stages of observation. However, towards the later stages, it was statistically on par with St. Vincent Red. Foliar spray was superior to rhizome dip in terms of total number of suckers, height of suckers and the number of leaves. In the case of leaf area, both the treatments were more or less on par. Application of growth regulators had pronounced effect on sucker production at all the stages during the first experiment when BA 750 mg l-1 produced the highest number (4.19) of total suckers. In the second experiment, variation was evident only in the total number of suckers. Here, BA 850 mg l-1 produced the highest number (4.33) of suckers and it was on par with BA 700 mg l-1 (4.00) and GA3 650 mg l-1 (3.79). Irrespective of the stage of plant growth, gibberellic acid produced taller suckers and BA 500 mg l-1 resulted in the shortest suckers. BA treatments recorded comparatively higher number of leaves. Growth regulator application had remarkably influenced the leaf area of suckers also. Application of BA 1000 mg l-1 (3.53 cm) and GA3 800 mg l-1 (3.33 cm) recorded higher collar girth in suckers. VG interaction exerted significant variation in the number of suckers. At varietal level, BA 750 mg l-1 produced the highest number of suckers in St. Vincent Red (4.75), GA3 500 mg l-1 in Golden Torch Adrian (4.63) and GA3 750 mg l-1 in Sexy Pink (4.00). Among VG treatment combinations in the second experiment, the highest number of suckers (4.75) in the variety St. Vincent Red was produced by BA 700 mg l-1. The varieties Golden Torch Adrian (4.88) and Sexy Pink (3.75) recorded the highest with BA 850 mg l-1. Gibberellic acid produced taller suckers. In the second experiment, VG interaction had no significant effect on the height of suckers. Regarding the number of leaves, BA resulted in comparatively higher number of leaves in both the experiments. In all the three varieties, BA 850 mg l-1 produced the highest number of leaves. Collar girth was found to increase with increase in the concentrations of BA and GA3 in all the three varieties. The economics of foliar application of growth regulators revealed that BA 850 mg l-1 significantly enhanced the profit in the varieties Golden Torch Adrian and Sexy Pink. Although negligible, BA 700 mg l-1 recorded slight positive response in the variety St. Vincent Red with respect to profit.
Open Access
Women empowerment through group action in the kudumbashree programme of kerala: a multidimensional analysis
(Department Of Agricultural Extension, College Of Agriculture, Vellayani, 2007) Priya Devi, R; KAU; Mothilal Nehru, S
The study was aimed to assess the extent of eradication of poverty and the dimensions of empowerment in the Kudumbashree programme of Kerala government for poverty alleviation. The determinants of performance of Self Help Groups organized in neighborhood basis and their functional and structural analysis including the tangible and intangible benefits and constraints were also studied. Suggestions to enhance the efficiency of the Self Help Groups(SHGs) in agriculture and other sectors organized under the Kudumbashree programme were made. A sample of 300 respondents was selected at random from three districts of three regions of the state namely Thiruvananthapuram from south Kerala, Ernakulam from central Kerala and Malappuram from north Kerala which were selected randomly. In each district, 25 SHGs comprising 20 non-agriculture and 5 agriculture oriented SHGs were selected. The structural and functional analysis of Kudumbashree was carried out by Mc Kinsey procedure of SWOT analysis. The implementing category of personnel namely top management, middle management and grass-root functionaries and the selected SHG members were the respondents for the study. The nature, extent and style of functioning of Kudumbashree were studied with the above respondents.Besides, all implementing personnel working in the above three districts were also selected. Interactions with state level management personnel were done to elicit information regarding the study and also for triangulating findings. A sub sample consisting of five poorly performing SHGs from agriculture and non-agriculture sectors making total of ten SHGs on the basis of perception of the implementing officials and the result of the study were selected for in depth sub sample analysis and their limitations were stated. In the study it was assumed that the dependent variables poverty eradication and women empowerment were interrelated with the performance level of the groups having an intervening effect .A measurement scale for PEI was developed to measure extent of poverty eradication which was one of the dependent variable as a specific objective of the study. Poverty eradication index was the yardstick or standard to measure how far the Kudumbashree has alleviated the incidence of poverty among the members .The index consisted of eight constitutive factors and the cumulative expression of the scores in relation to the factors is the poverty eradication index of the respondent. The data was gathered by using the pre tested interview schedule / questionnaire developed for the study. Statistical tools used in the study were quartile deviation, mean, ANOVA and correlation analysis. Poverty eradication index score and empowerment score were given in quartile range to show the extent of poverty eradication and empowerment attained by the Kudumbashree programme. Categorisation was done in low, medium, high and over. As recorded in the study, regarding health care majority of the respondents were dependent on primary health care centre and the constraints felt were lack of supply of medicines, doctors not being available for ensuring timely medical care and the primary health centre located far away from the home. Nature of savings of respondents were with majority having group savings while post office savings, insurance and chitties were also reported.Borrowing pattern regarding the source of borrowing by the respondents showed only reliable institutional sources were used to avail loans by the majority.For purpose of borrowing repayment of debt was recorded as the highest felt need requiring borrowings followed by shelter renovation activities and health care need. Regarding the confidence of repayment majority reported they were confident of repayment while borrowing the amount. But 270 of them had experienced instances of inability to repay on time. 276 respondents of them had felt mental stress due to non repayment of borrowed amount on time. Access to safe drinking water and the source for drinking water as recorded in the study had half of the respondents having access to safe drinking water in their house premises itself. Regarding the vulnerability status of the respondents it was seen that majority of them had faced a situation were they have been unemployed for a month or more. They took loan or lent out assets in the situation. Regarding alcoholism 120 of respondents agreed that the earning male member of the family was alcoholic. Control over earnings had majority satisfied with the expenditure pattern of the family. Regarding entrepreneurial culture 60 of respondents had a temporary job while 99 had permanent job. 75 had reported to go to job daily while 60 had job availability sometimes only. All respondents had reported that they think the work undertaken by them was respectable in society. According to perception of respondents, repayment of loan and not possessing own home were the major factors of poverty while risk factors were damaged house or having no own house. The next constraint was recorded for having no or only one regular employed person in the family. Poverty eradication index ranged from a minimum of 19.51 in all three districts to a maximum of 60.53 in Thiruvananthapuram and 65.76 in both Ernakulam and Malappuram .The mean index was 41.26 in Thiruvananthapuram, 43.92 in Ernakulam and 41.07 in Malappuram. Thus a wide variation in the extent of poverty eradication was shown .The activities will have to be strengthened further to ensure appreciable alleviation of poverty. Empowerment index recorded a minimum of 29.70 in all three districts to a maximum of 86.07 in Thiruvananthapuram and 77.68 in both Ernakulam and Malappuram .The mean index was 55.10 in Thiruvananthapuram, 53.03 in Ernakulam and 51.36 in Malappuram. Regarding relationship between poverty eradication index and empowerment index, all three districts showed significant relationship in this study.Analysis of Variance (ANOVA) test showed significant results for empowerment index. Inferences from categorization of groups in the districts based on group performance index were that medium level of group performance was shown by majority of groups. In depth sub sample analysis had the following limitations identified. For the group doing rice farming, the owners are reluctant to lease out every season and processing units and storage facilities were absent. The members of the computer units had to meet extra expenses of electricity bill and the rent for the building from the income. Delay in payment of works undertaken for public purposes was reported. Marketing is a difficult task for coconut oil and for pappad making units.Drudgery, improper risk management, improper auditing and absence of diversification of crops were also reported. Design of the action program was done with formulation of plan for vegetable cultivation with College of Agriculture,Vellayani as source to get timely information, inputs and technical support.Execution of action program was done and impact assessed by proper monitoring of the process, identifying technological constraints with its solutions. SWOT analysis of Kudumbasree program showed that the localised social upliftment measures by community based organization, enhanced participation through meetings at home and the facilitation of women to enter public life was the major strengths. Productive usage of loan amount through canalised allotment of resources and holistic identification of poor not based on income/consumption but based on risk factors of poverty were the major strengths of the programme. The weaknesses of the programme recorded were leadership being influenced by political interference leading to corruption in work culture and intra group conflicts have been reported to limit the success of certain enterprises especially incase of consumptive usage of credit in absence of proper supervision. Lacks of enthusiasm among young members, cases of late approval of projects or absence of skill development for increasing the market competency were noticed. The opportunities of Kudumbasree program were establishment of social, security network for destitute rehabilitation and to increase the level of participation of members. The preparation of micro plan using the risk indices and problem identification mechanism were also opportunity. Small enterprises can be used for diversification and modernisation of rural economy and skill up gradation of general poor population. The threats include absence of quality control for ensuring market competency, dissipating enthusiasm and lack of proper tie up with line departments .Lack of autonomy in policy formulation lead to micro plan being not given due consideration. Charge officers are not provided technical up gradation for supporting the enterprises and the products lacked local market demand. Tangible and intangible benefits identified in the study were extension of decentralization process to further grass root levels, enhanced female control over earnings, diversification and modernisation of rural economy, sustainable maintenance of Kerala model of development, dependable and trust worthy social network generated, and the reduction in social discrimination and social evils. At the level of implementation of Kudumbasree program benefits were enhanced production lending in the groups than consumption lending, non economic risk indices in identification of the beneficiaries, the multifaceted frame work to alleviate root causes of poverty , reduction in under employment and educated unemployment followed by the provision of separate administration over centrally sponsored programs . The benefits at beneficiary level included constituting a working group for poverty reduction and the habit of savings first and credit later aiding to self dependence. The existence of a group fund gave members experience in money management and trust in people ensure repayment. Also enhanced food security by undertaking rice farming, participatory need based decision making and the ability of NHGs to act as a forum to share concern were other benefits. The major constraints in planning level were panchayaths not creatively participating in project design and micro enterprise planning followed by lack of symbolic policy formulation from part of State Government. Regarding the implementation level the major constraint was recorded as no remuneration paid for the activities undertaken by ADS and CDS Secretaries is followed by the enterprises not being matched with market demands or incompatible to daily routine of members. Mixing up the poor and non- poor in groups was a sure path to failure. Also some beneficiaries sell the productive assets for immediate financial benefit which limits timely repayment. Suggestions on the basis of the study were that rejuvenation and intensification of the Kudumbashree need appraisals and campaigns to limit exploitation in a target oriented manner. Policy formulations from State Government for investment in social capital have to be given more priority; sales promotion must be undertaken widely assisted with innovative enterprise planning and implementation. Technical support for finance management and common supply of raw materials like Bamboos at reasonable cost should be ensured. The stationery supply for canteens can also be organised in common basis by using the wide network generated. The link agents between NHGs and CDS should be ensured proper payment. Sustainability measures including marketing outlets and reduction in drudgery, the schedule for loan recovery specific to enterprise according to the pattern of returns and prevention of sale of assets purchased must be ensured. Proper representation of general problems by consolidation and prioritisation of such ideas to micro plans should be undertaken. More profitable enterprises involving more members by sharing of experiences must be done. Incentives for timely repayment, proper guidance and conflict management cell should be ensured. Suggestions to improve functioning of agriculture oriented groups is to develop a regulated system to pass on specification of the orders to units, timely supply of quality inputs like raw materials through proper channels should be undertaken. Documentation of activities can organize the activities in a scientific manner. Interested members should be motivated by trainings. Small scale units of diverse products with market demand like medicinal plants, vermicomposting and mushroom culture should be organized widely. Proper grading, hygiene and attractive packing to ensure market competency should be done within the local infrastructure available.
Open Access
Heterosis breeding in sesame (Sesamum indicum L.).
(Department of Plant Breeding and Genetics, College of Horticulture, Vellanikkara, 2011) Gayathri, G; KAU; Dijee, Bastian
The study entitled ‘Heterosis breeding in sesame (Sesamum indicum L.)’ was undertaken at the Department of Plant Breeding and Genetics, College of Horticulture, Vellanikkara. The objectives of the study were to collect and evaluate different genotypes of sesame for morphological traits and yield attributes, to identify useful parents producing heterotic crosses and developing hybrids in sesame. The study also intended to develop male sterile lines in sesame through interspecific hybridization with Sesamum malabaricum. Sesamum indicum and Sesamum malabaricum accessions were collected from Kerala and Tamil Nadu and evaluated for their morphological traits. Wide range of variation was noticed for characters like plant height, number of days to flowering and seed yield per plant which contributed maximum to genetic divergence. The genotypes studied were grouped into six clusters. High genotypic coefficient of variation (GCV) was recorded for number of capsules per plant, plant height, seed yield per plant and number of branches per plant. High heritability with high genetic advance as per cent of mean was recorded for number of days to flowering, plant height, number of branches per plant, number of capsules per plant and seed yield per plant. This indicates that the characters are governed by additive gene effects and selection for these traits will be effective. Association analysis revealed that seed yield per plant was correlated to plant height, number of capsules per plant and number of days to flowering. Path coefficient analysis indicated maximum positive direct effect by number of capsules per plant, capsule length, plant height and 1000 seed weight on seed yield per plant. In order to develop hybrids, fourteen parents were selected based on the per se performance of the genotypes. They were crossed in line X tester mating design. Forty eight hybrid combinations obtained were raised in the field along with the parents and evaluated for their heterosis and combining ability effects. Parental genotypes AVTS-06-5, AVTS-06-10, IVTS-06-12, KYM-1, Tilak and TMV-6 were identified as high combiners based on general combining ability (gca) effects. Two combinations viz. AVTS-06-5 X KYM-1 and IVTS-06-12 X TMV-3 had significant values of per se performance, specific combining ability (sca) effects and standard heterosis for seed yield per plant. They can be evaluated for their hybrid vigour over locations and seasons. The crosses AVTS-06-5 X TMV-3, AVTS-06-5 X TMV-6 and TCR 3279A X KYM-1 have been identified as potential cross combinations for isolation of promising segregants as the parents involved in these crosses had high significant gca effects for seed yield per plant but the hybrids recorded non significant sca effects. Interspecific hybridization between S.malabaricum and S.indicum was attempted to develop male sterile lines. Seed set was noticed in three interspecific hybrids which failed to germinate due to embryo abortion. Hence these embryos were rescued and raised in vitro to obtain the hybrids.

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