Theses

Browse

Search Results

Now showing 1 - 9 of 152

Open Access
Immunodiagnosis of bovine gastrothylacosis using coproantigens
(Department of parasitology, College of Veterinary and Animal Sciences, Mannuthy, 2000) Kandasamy, A; KAU; Devada, K
A study was conducted on the prevalence of paramphistomatidosis in Thrissur from June 1999 to May 2000, feasibility of coproantigen detection by ELlSA and comparison of sensitivity of ELlSA using coproantigens and ELlSA using serum antibodies in diagnosis of gastrothylacosis, caused by Gastrothy/ax crumenifer, in cattle. It was noted from the registers maintained at the University Veterinary Hospitals at Kokkalai and Mannuthy and that at the Department of Veterinary Parasitology, College of Veterinary and Animal Sciences, Mannuthy, that out of a total number of 1534 faecal samples from bovines examined, 253 (16.5 per cent) animals were found to be positive for amphistome eggs with the maximum prevalence (23 per cent) in June and July. Generally the infection was prevalent throughout the year. An indirect ELlSA using rabbit hyperimmune serum against somatic antigens of G. crumenifer was performed to detect coproantigens in faecal samples collected from 100 known G. crumenifer infected cattle. Seventy four samples were found to contain detectable levels of coproantigen indicating a sensitivity of 74 per cent. Serum samples collected from the same infected cattle were tested for antibodies to G. crumenifer by an indirect ELlSA using somatic antigens. Fifty one samples were found positive for antibodies indicating a sensitivity of 51 per cent. It was seen that when 43 cattle were positive for both coproantigens and serum antibodies, 18 cattle were negative for both of them. Although 31 cattle which were negative for serum antibodies were found positive for coproantigens, eight cattle negative for coproantigens were found positive for serum antibodies. The results showed that coproantigen detection, which revealed a higher sensitivity than the detection of serum antibodies by ELlSA, is feasible for the diagnosis of gastrothylacosis in bovines.
Open Access
Developing and testing of a collector-cum-storage types solar water heater for domestic use
(Department of Farm Power Machinery and Energy, Kelappaji College of Agricultural Engineering and Technology, Tavanur, 2000) Bijukumar, K; KAU; Mohammad, C P
The present study was undertaken to desi~n and develop a collector-cum-storage type solar water heater of 30 litre capacity to supply hot water for domestic use. Five solar water heaters with different absorber plate positions were constructed. The storage tanks with dimensions of 67 xIl7 x 10 cm were made by using fibre glass, and the top of these storage tanks were covered using single plain glass plates of 71 x 51 x 0.4 cm size. The position of the absorber plate was varied by changing the width of the spacers (4 nos. at an angle of 400 with the side aluminium sheet), which connects bottom aluminium plate and top absorber plate. The absorber plate was of 65 x 45 cm size. The top face of the absorber plate was painted black to absorb maximum solar. radiation. The absorber plate positions were lcm, 3cm, 5cm, 7cm and 9cm from the top glass cover plate and were designated as SWHl 0, SWH30, SWH5.0, SWH7.0 and SWH9.0 respectively. The absorber plate position was optimized by testing the solar water heaters under two different test conditions. Solar water heater II (SWH3.0) outperforms other solar water heaters under the two test conditions. Maximum outlet temperature of 64°C af 3pm and maximum efficiency of 55.72% also at 3pm were observed in solar water heater 11. So it is optimized that the position of the absorber plate should be at 3cm from . the top glass plate. The solar warer heater can easily be handled by a single person since the weight is only 9.5kg. The operating cost per unit of thermal energy obtained withthe solar water heater was found to be 25 paise per kWh.
Open Access
Irrigation scheduling and moisture conservation in water melon
(Department of Agronomy, College of Horticulture, Vellanikkara, 2000) Ajith, C B; KAU; Joseph, P A
An experiment was conducted at the Agricultural Research Station, Mannuthy, Thrissur during the period from December 1998 to March 1999 to study the influence of levels of irrigation and moisture conservation methods on the growth and yield of water melon (Citrullus fanatus) grown in rice fallows. The experiment was laid out in randomized block design with three replications. The treatments consisted of combinations of four moisture conservation methods (control, application of paddy waste as surface mulch, uniform incorporation and bottom layer application @ 1/3 pit volume) and four levels of irrigation (IW /CPE ratio of 0.6, 1, 1.4 and farmer's practice of daily irrigation @ 20 lit pit"), The study revealed that incorporation of moisture conservation materials increased the growth attributes like length of vine, number of leaves per vine, leaf area and dry matter production and yield attributes like weight of fruits and number of fruits per plant. Among moisture conservation methods surface mulch and uniform incorporation of paddy waste were found to be superior in most of the cases. Watermelon responded very well to irrigation. Biometric characters (length of vine, number of leaves per vine, leaf area and dry matter production) and yield attributing characters (weight of fruits and number of fruits per plant) were favourably influenced by frequent irrigations. The fruit yield increased with increase in frequency of irrigation and was maximum at IW /CPE ratio of 1.4. Results of soil moisture studies revealed that incorporation of moisture conservation material' increased the soil moisture content, consumptive use and water use efficiency. The consumptive use increased with the frequency of irrigation. The top 15 cm of the soil layer accounted for the highest soil moisture depletion. The depletion was more from the deeper layers in the drier regimes. Field and crop-water use efficiencies were higher in less frequently irrigated treatments. Higher levels of irrigation increased N, P and K content of leaves. No convincing change in leaf N, P, K content could be brought about by the incorporation of paddy waste. Economic analysis showed that incorporation of paddy waste could increase the net profit. Net profit as well as net return per rupee invested were the highest with scheduling irrigation at IW/CPE ratio of 1.4. Among the treatment combinations, the best combination was surface application of paddy waste with irrigation at IW/CPE ratio of 1.4 followed by the combination of surface mulching and fanner's practice of daily irrigation.
Open Access
Character association of seeds on plant morphology in snakegourd (trichosanthes anguina L.)
(Department of Olericulture, College of Horticulture, Vellanikkara, 2000) Ashok, P; KAU; Rajan, S
The research project 'Character association of seeds on plant morphology in snakegourd (Trichosanthes anguina L.)' was carried out in College of Horticulture, Kerala Agricultural University, Vellanikkara, Thrissur during the period 1999-2000. The study envisaged to find out the association of various seed characters on the vegetative characters of snakegourd, classification of accessions based on the seed index, growth index and yield index as obtained from principal component analysis and to formulate keys for varietal identification. The 25 accessions were catalogued based on the IBPGR descriptor list. Significant differences for various seed characters, growth characters and yield characters were noticed among the accessions. Correlation studies have shown that the number of seeds per fruit strongly associated with days to male flower opening and fruit maturity. Seed size index was strongly associated with leaf size. The highest seed index value for TAl 08, the highest growth index value for TA 118 and the highest yield index value for TA 114 were obtained as revealed from principal component analysis . . Twenty five accessions were grouped into different clusters based on the seedindex, growth index and yield index. Based upon the seed characters (seed size, seed coat colour and 100 seed weight), growth characters (leaf lobes, leaf pubescence and tendril length) and fruit characters (fruit skin colour, fruit girth, fruit length and fruit weight) keys were developed for varietal identification.
Open Access
Analysis of animal husbandary information in the farm feature pages of leading malayalam dailies
(Department of Extension, College of Veterinary and Animal Sciences, Mannuthy, 2000) Sathumadhavan, T P; KAU; Subhadhra, M R
Study was a content analysis of animal husbandry information in farm . feature pages of four Malayalam dailies, namely Malayala Manorama, Mathrubhoorni,. Desabhimani and Deepika, for the period January to December 1999. Variables studied were space provided to different categories of animal husbandry news, modes of presentation, frequency of occurrence of news, seasonality, readability, relevancy and accuracy. Research findings revealed that the studied dailies provided less than one fourth space of farm feature pages for publishing animal husbandry information. Large animal category got more than 50 per cent coverage with three-fourth space occupied by articles on cows. Poultry articles occupied one-fourth space of animal husbandry information of farm feature pages. Where as small animals and pet animals occupied 17 per cent and seven per cent space respectively. News articles occupied maximum column length in all dailies studied. Frequency of occurrence of news articles, illustrations and question answers were found to be more when compared to other modes of presentation. Seasonality studies r.evealed that more than half the number of dairy articles and one eighth of poultry articles were seasonal. Average readability of dairy articles according to Fog index was 5.45 with more reading easiness. More than 50 per cent of dairy articles were found to be relevant. Just above two-third of scientific messages of dairy articles were accurate, whereas accuracy of general articles were found to be nearly 50 per cent.
Open Access
Effectiveness of instructor controlled interactive video (ICIV) in dissemination if farm technology
(Department of Agricultural extension, College of Horticulture, Vellanikkara, 2000) Radhakrishnan, D; KAU; Ranjan Karippai, S
The study was aimed at bringing out the effectiveness of Instructor Controlled Interactive Video (ICIV) in popularising agricultural technologies among the farming community. The impact of the different treatments of ICIV with different video formats was also experimented. The study was conducted among 150 vegetable growers from Nadathara grama panchayath of Thrissur district. The respondents were farm women cultivating vegetables. IPM in vegetables was chosen as the message to be disseminated. The dependent variables of the study were knowledge gain and symbolic adoption of IPM practices in vegetables. The set of 11 independent variables chosen covered the psychological and situational factors of the respondents. The distribution of. respondents based on knowledge gain and symbolic adoption showed that majority of respondents were in high category after participating in ICIV. Out of 11 independent variables studied six variables were correlated significantly with knowledge gain. Five variables showed non significant relationship. Multiple regression analysis showed that 62 per cent variation in knowledge gain was contributed by the independent variables. Correlation analysis of independent variables with symbolic adoption showed that five independent variables had a positively significant relationship with symbolic adoption. One variable was negatively significant with symbolic adoption. Remaining five varied showed non significant relationship. Multiple regression analysis concluded that 59.2 per cent variation in symbolic adoption was brought about by related independent variables as a whole. The significant 'F' value indicated that all the treatments were effective in terms of knowledge gain and symbolic adoption. Students 't' test was carried out to compare the treatments. ICIV with 'Feature' mode of video presentation and ICIV with 'Skill demonstration' mode of video presentation were the most effective modes in maximising knowledge gain and symbolic adoption.
Open Access
Parasexual hybridization of piper nigrum and piper colubrinum through protoplast fusion
(Department of Plantation Crops And Spices,College of Horticulture, Vellanikkara, 2000) Resmi, Paul; KAU; Rajendran, P C
Black pepper is the most important export oriented commodity and foreign exchange earner among the Indian spices. Ravages due to diseases, particularly the most devastating Phytophthora foot rot caused by Phytophthora capsici is one of the major constraints in the production of black pepper all over the world. Piper colubrinum, a wild relative of black pepper is found to be immune to foot rot disease. Non – existence of cultivar level tolerance or resistance against foot rot disease in black pepper necessitated the incorporation of incompatible wild relatives through parasexual hybridization. This study was undertaken in the Department of Plantation Crops and Spices and the Plant Tissue Culture laboratory of the Centre for Plant Biotechnology and Molecular Biology, College of Horticulture, Vellanikkara between September 1998 to April 2000. The cultures of P. nigrum and P. colubrinum were initiated in test tubes and were maintained at 26 + 20C temperature and 60 to 80 per cent humidity. The green leaves excised from axenic cultures of both the species were treated with cell wall degrading enzymes, cellulose and pectinase maintained at proper osmotic concentration. In P. nigrum maximum yield was observed at 1.4 per cent cellulose and 0.34 per cent pectinase. Cellulase and pexctinase at a concentration of 1.0 per cent and 0.217 per cent respectively recorded highest yield in P. colubrinum. In both the species 0.6 M osmoticum was found to be optimum to maintain the osmotic potential of the isolation solution. Highest yield of protoplasts was recorded in both the species during 21 h of digestion. Filtration-centrifugation technique was found to be superior in purifying the Piper protoplasts compared to the sucrose floatation method. Centrifugation at 1000 rpm for three minutes was found to be best for purifying P. nigrum protoplasts. For purifying P. colubrimum protoplasts, 600 rpm for three minutes was found to be optimum. Highest viability was noticed at 0.55 M and 0.65 M osmoticum in 1.0 per cent cellulose and 0.28 per cent pectinase during 21 h of digestion in P. nigrum. In P. colubrinum, maximum viability was observed at 0.4 M osmoticum in the enzyme mixture 1.0 per cent Cellulase and 0.186 per cent Pectinase during 18 h of incubation. Protoplasts of both the species when cultured on modified MS medium formed no cell wall and have not undergone any division. A age of the cultures advanced, proptoplast viability decreased in P. nigrum and P. colubrinum. All the protoplasts died by the second week in both the species. The protoplasts of both the species were heterogenous in terms of size. Fusion of the protoplasts was not observed after PEG treatment in the present study.
Open Access
Heterosis and combining ability in melon
(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 2000) Deepthy, R; KAU; Wilson, D
The current research programme on 'Heterosis and combining ability in melon (Cucumis melo (L.) var. conomon) was carried at the Department of Plant Breeding and Genetics, College of Agriculture, VeIl ay ani during 1998- 2000. The objectives were estimation of various genetic parameters, heterosis and combining ability effects of parents and hybrids. The experimental material consisted of seven parents and their 21 F 1 hybrids (without reciprocals). Analysis of variance revealed highly significant difference among the genotypes for all the 21 characters studied. The genotype P3 topped in mean performance for yield and many yield attributes. The hybrids P2 x P6 and P2 x P3 were superior in yield and fruit characters, whereas P6 x P7 was the best for keeping quality, yield and crop duration. Genetic parameters like PCV, GCV, heritability and genetic advance were high for keeping quality, yield, sex ratio and mean fruit weight. Environmental component of variation was negligible for majority of traits. Significant heterosis was observed for all the characters studied. P3 x P 4 showed maximum standard heterosis for yield whereas, P2 x P 6 showed maximum relative heterosis and fruit characters and earliness traits. Variance due to GCA and SCA were significant for all the traits studied. P3 was the best general combiner for yield, fruit diameter, mean fruit weight and fruit length and P2 was the best for node characters and crop duration. The hybrid P6 x P7 was the best specific combiner for yield. Combining the mean performance, SCA effects and standard heterosis, P2 x P6 was identified as the superior cross in terms of yield and yield attributes whereas P 4 x Ps was the best for various flowering traits.
Open Access
Physiological aspects of ex vitro establishment of tissue cultured orchid plantlets
(Department Of Plant physiology, College Of Agriculture, Vellayani, 2000) Samasya, K S; KAU; Viji, M M
The present investigation was undertaken to elicit information on the physiological, morphological, biochemical, biometric and anatomical changes during in vitro propagule multiplication and ex vitro establishment in tissue cultured plantlets of orchids. Dendrobium hybrid Sonia 17 was the variety used for the study. The rooting media was supplemented with different levels of sucrose. At the time of planting the plantlets were subjected to triazole treatment. During the process of hardening the plantlets were maintained in hardening chambers with different levels of light and humidity. Among growth parameters the crop growth rate (CGR), net assimilation rate (NAR) and relative growth rate (RGR) were found to be high at 40 gIl of sucrose concentration. The CGR of these plantlets were on par with normal green house grown plantlets. The photosynthetic rate was found to increase and the transpiration rate was found to decrease at 40 gIl of sucrose . concentration. The maximum survival percentage of the in vitro plantlets occur when 40 g11 of sucrose incorporated in the rooting medium. This may be attributed to the influence of the sucrose concentration on morphological characters studied viz. plant height, number of leaves per shoot and number of roots per shoot. With regard to photosynthetic pigments an increase in the content of total chlorophyll, chlorophyll a chlorophyll b and carotenoids occurred in plantlets treated with 40 g/1 of sucrose. Also the protein content and carbohydrate content was maximum at the above sucrose level. The leaf area, root length, total fresh weight and dry weight of the plantIets maintained at 40 gIl of sucrose level were higher than other treatments. These effects ultimately lead to better survival percentage. Triazole treatment of plantIets during planting out helped in better survival percentage. With regard to the growth parameters the effect was distinct towards the later stage of growth. CG~ NAR and RGR were maximum on the triazole treated (5 mgll) plantIets and was comparable to normal green house grown plants. There was marked increase in the photosynthetic rate and decrease in transpiration rate of plantIets treated with 5 mgll of triazole. However the photosynthetic rate of normal green house grown plants were much more than the tissue cultured plantIets. Regarding morphological characters, increasing concentration of triazole had negative influence on plant height. However plant height of normal green house grown plants were distinctively higher than tissue cultured plantIets. With regard to number of leaves per shoot, triazole treatment showed significant effect only after 30 days of planting out, whereas the number of roots per shoot was very much influenced by triazole. The maximum number of roots was produced at 5mgll of triazole treatment and these effects in turn influenced higher survival percentage of plantIets. ------....- ....- ~ - - - ----- Regarding the biochemical aspects total chlorophyll, chlorophyll a, chlorophyll b and carotenoids contents of plantlets treated with triazole (5 mg/l) were higher and found to be on par with that of green house grown normal plants towards the later stage. The protein content was also positively influenced by triazole treatment (5 mgll) and the value was comparable to that of normal plantlets. In the case of carbohydrate content the treatment becomes statistically significant and the normal green house grown plants exhibited distinctively higher value. The plantlets treated with 5 mgll of triazole were found to have higher leaf area, root length, root: shoot ratio, total fresh weight and dry weight than other treatments. However the root shoot ratio and total fresh weight of treated plantlets were on par with the normal green house grown plants towards the later stage viz, 45DAP. The physiological, morphological, biochemical and biometric characters of the plantlets were also found to be influenced by different levels of light intensity and humidity maintained in the hardening chamber. Among the different treatments, plantlets grown at 50 percent light intensity and 70 to 90 percent relative humidity exhibited higher CG~ NAR and RGR. Also these plantlets exhibited a marked increase in photosynthetic rate and decrease in transpiration rate. However CGR and NAR of green house grown normal plants were distinctively higher during the later period. However the normal plants had distinctively higher CG~ RGR and photosynthetic and very less transpiration rate than other treatments. The plantlets grown under 50 percent light intensity and 70 to 90 percent humidity produced maximum plant height, number of leaves and number of roots per shoot. The survival percent of the tissue cultured plantlets in the field condition (green house) was found to be superior under appropriate environment of light and humidity (50 percent light intensity and 70 to 90 percent relative humidity) There was not much significant difference among the treatments of light and humidity in the case of pigment content. However the plantlets grown in the hardening chamber maintained at 50 percent light intensity and 70 to 90 percent of relative humidity recorded maximum value of total chlorophyll, chlorophyll a, chlorophyll b and carotenoids among treatments and the normal green house grown plants exhibited distinctively higher pigment content. The normal plantlets recorded marked increase in protein content and carbohydrate content than the tissue cultured plantlets. In the present study, an environment of 50 percent light intensity and 70 to 90 percent relative humidity was found to be superior and could favour enhanced leaf area, total fresh weight, total dry weight and root length of plantlets which ultimately resulted in better survival percentage. The in vitro plantlets observed to have anatomical characters as compared to the normal green house grown plants and hardened plantlets. The stomata remained open and less number of stomata per unit area of leaf was observed as the in vitro leaves were concerned. Another peculiarity of the in vitro plantlets was the absence of cuticle layer. The mesophyll layers were also found to be less compared to normal plantlets. These anatomical characters were one of the severe limitations of the micropropagated orchid plantlets during ex vitro establishment which ultimately resulted in high rate of field mortality.

Theses

Browse

Thesis Type

Type

Author

Date

Subject

Search Results