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ThesisItem Open Access Certain haematological parameters and blood biochemical constituents in cows with normal and impaired fertility(Department of Animal Reproduction, College of Veterinary and Animal Sciences, Mannuthy, 1993) Ani S, Das; KAU; Neelakanta Iyer, C PA study was conducted to asses the influence of certain haematological parameters like haemoglobin (Hb) and packed cell volume (PCV) and biochemical constituents like total serum protein, serum phosphorus, total blood cholesterol and blood glucose in crossbred cows and the efficacy of replacement therapy of glucose and phosphorus on fertility in deficient animals. Animals which had apparently normal breeding history and conceived with one or two inseminations were classified into group I. Animals that were hard to settle and apparently deficient in nutrition but clinically free from any genital affections were classified into group II. Blood samples were collected from all these animals at oestrus and levels of Hb, PCV, serum total protein, serum phosphorus, blood glucose and total blood cholesterol were estimated. After estimation of blood constituents the cows in group II were divided into two. Animals in group II A were inseminated without any treatment and animals in group II B were administered 15 ml of Tonophosphan by intravenous injection or 540 ml of 25 per cent dextrose as intravenous infusion or both based on the deficiency of phosphorus or glucose alone or in combination. The levels of Hb, PCV, serum phosphorus, blood glucose, total serum protein and total serum cholesterol were 10.06+0.06 g per cent, 31+1.33 per cent, 4.85+0.343 mg per cent, 46.10+3.19 mg per cent and 7.00+0.99 g per cent and 95.09+8.67 mg per cent respectively in normally fertile cows. In cows with impaired fertility which were deficient in different constituents the corresponding levels were 6.86+0.75 g per cent, 21.50+0.50 per cent, 3.65+0.068 mg per cent, 31.13+0.726 mg per cent, 4.57+0.30 g per cent and 71.36+1.66 mg per cent respectively, all of which were significantly lower than their corresponding levels in normally fertile cows (P < 0.10). The overall conception rate was 81.80 per cent 11.11 per cent and 77.77 per cent in group I, group I IA and II B cows. Conception rate of cows with lower levels of Hb (zero) was significantly lower than the conception rate (50 %) observed in animals with normal level of Hb (P<0.05). Similarly the same in cows with normal and low levels of PCV was 40.75 per cent and zero respectively. Cows with low levels of serum protein and serum phosphorus had conception rate of 40.70 per cent and 8.30 per cent against 46.70 per and 71.40 per cent in animals with normal values. Statistical analysis revealed significant difference in the case of phosphorus (P<0.01). Similarly cows with low levels of blood glucose and total cholesterol also had significantly low (P<0.01) conception rates (7.69 and 13.33% respectively) as against that in normal cows (70 and 69.20%). Animals when treated with phosphorus alone 71.42 per cent conceived as against 25 per cent in non treated group. Similarly 75 per cent of animals conceived when treated with glucose compared to 12.5 per cent conception rate in non treated group. When cows were given combined treatment of glucose and phosphorus conception rate was 87.5 per cent while none conceived in non treated group. This difference was statistically highly significant (P <0.01).