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Open Access
Tracheal reconstruction in dogs under acepromazine - thiopental anaesthesia
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1995) Angamuthu, Jayasudha; KAU; Ravindran Nayar, S
The experiment was conducted on twelve, apparently healthy, adult, nondescript dogs of either sex, divided into two groups, viz., Group 1 and Group 11, each consisting of six animals. Circumferential resection of two adjacent tracheal rings of the cervical trachea was performed and the trachea was reconstructed by end – to – end anastomosis in the animals of Group 1 and with Marlex mesh prosthesis in the animals of Group 11. All the animals were premedicated with acepromazine maleate IM, and anaesthesia was induced by 2.5 per cent solution of thiopentone sodium IV. Induction of anaesthesia was complete by 3.26 + 0.10 minutes, duration of surgical anaesthesia was 65.00 + 3.29 minutes and time for recovery was 192.91 + 13.68 minutes. Variation in the physiological and haematological parameters during anaesthesia were not significant. In Group 1, all the animals had normal respiratory function throughout the period of observation, following surgery. In Group 11, all the animals, except one, developed severe complications and died within one to four weeks postoperatively. Only one dog survived in this group and was sacrificed on the 45th postoperative day. During the postoperative period, the rectal temperature did not show marked variations in both the groups. The pulse and respiration rates showed an initial increase in Group 1. However in Group 11, marked decrease in pulse rate and increase in respiration rate was noticed. Hemogram on the different postoperative days showed an increase in the total leucocyte count in both the groups, and increase in monocyte and eosinophil count in Group 11. Radiography on different postoperative days in Group 1 demonstrated that there was no reduction in the size of the tracheal lumen at the site of anastomosis in five of the six animals. In Group 11, radiography revealed a progressive reduction in the size of the tracheal lumen at the site of reconstruction in four animals, and only slight reduction in one dog on the 45th postoperative day. At autopsy, gross examination of the trachea at the site of anastomosis in Group 1 showed mild to moderate adhesions to the adjacent tissue and there was no reduction in the size of the tracheal lumen in five of the six dogs of this group. In animals of Group 11, dense adhesion between the site of reconstruction and adjacent tissue was observed. The mesh was fully incorporated at the site of reconstruction in five of the six animals. One animal had shown anastomotic dehiscence. Almost complete occlusion of the trachea by overgrowth of tissue was observed in four animals and slight reduction in the tracheal lumen in one animal. Histopathology at the site of anastomosis in Group 1 revealed complete healing of all the layers of the trachea by the 30th postoperative day. In Group 11, tracheal stenosis was associated with ingrowth of granulation tissue in four animals. The mesh was infiltrated by fibrous tissue in five of the six animals. Epithelium was seen lining the prosthesis on the 45th postoperative day.
Open Access
Acrylic external skeletal fixator for the treatment of long bone fracture in dogs
(Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Science,Mannuthy, 2005) Julie, B; KAU; Syam Venugopal, K
The efficacy of replacing stainless steel connecting bar in external skeletal fixator (ESF) with acrylic connecting bar was evaluated by using acrylic connecting bar in twelve clinical cases of complete fracture of long bones in dogs presented to the Surgery Units of Veterinary Hospitals of Mannuthy and Kokkalai, College of Veterinary and Animal Sciences, Mannuthy, during the period of December 2003 to May 2005. All the animals were subjected to detailed clinical, radiological, haematological and serum biochemical evaluations before application of acrylic fixator and also postoperatively at two weeks interval upto sixth week or until the removal of the implant. Type IA or type II acrylic fixators were applied by closed or open approach under general anaesthesia depending on the type of fracture. Transfixation pins were drilled and the acrylic connecting bar was connected directly or following the application of a temporary stainless steel connecting bar, which was removed later. Fixator with acrylic connecting bar on one side and stainless steel connecting bar on the other side was used in three animals. Acrylic external fixator proved to be an economical, technically feasible, clinically successful and reliable alternative for stainless steel external fixators for the immobilization of fractures of radius and ulna and tibia and fibula in animals of less than 15 kg body weight. In heavier animals, usage of acrylic bar on one side of the type II fixator gave adequate stability in case of radial fractures but not for tibial fractures. Early return of sound functional limb usage following fixation with acrylic ESF was remarkable. All the animals, except three, could make slight ground contact with the fractured leg by the third post operative day and had apparently normal gait by the fourth week of observation with full weight bearing on the limb. Loosening of the proximal most pin occurred in Case Nos. 2, 3 and 4, by fourth week of observation, where type I acrylic fixator was used, but none of them affected the fracture healing significantly. Breakage of acrylic bar occurred in Case Nos. 7 and 9. In Case No. 7, the acrylic bar failed to tolerate the strong muscle pull on the fractured femoral fragments and in Case No. 9 severe mutilation by the animal resulted in breakage of the bar. Four animals exhibited mutilation on the implant, but only one on them showed severe mutilation. Mild pin tract drainage occurred in four animals and pin tract sepsis resulted in one animal. The heat generated during exothermic phase of acrylic hardening produced no apparent thermal necrosis of bone or soft tissue. Mild to moderate degrees of malalignment occurred following application of acrylic fixator in Case Nos. 3, 5, 6 and 11, which got nullified with progressive callus formation and resulted in restoration of normal straight line alignment of the bone. Marked angulation of the bone fragments occurred in Case Nos. 2 and 9. The fracture gap in all the cases was found to be progressively getting filled up with callus. Rate of callus formation varied with age of the animal, type of fracture and stability of the apparatus. In 50% of the cases, the fracture healed with endosteal callus only, while it healed with endosteal and periosteal callus in rest of the animals. Periosteal reaction of varying degree occurred in most of the cases but did not affect fracture healing or functional limb usage. Osteolysis was noticed around proximal pin tract in four animals, which could be due to loosening of pins. However, no significant alteration in fracture healing was produced. Acrylic column of one centimetre diameter was found sufficient for use as connecting bar of ESF for immobilization of fractures of radius and ulna and tibia and fibula in animals of less than 15 kg body weight.
Open Access
Cryosurgical treatment for experimentally induced cataract in dogs 260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
(Department of Surgery, College of Veterinary and Animal Sciences,Mannuthy, 2000) Neelakanta Praveen, Pillai; KAU; Ravindran Nair, S
This study was conducted with the objective of comparing the efficacy of cryo-coagulation and intra-capsular cryo-extraction of experimentally induced cataract in dogs. Twelve nondescript dogs aged approximately one year were used for the study in two groups, A and B, each consisting of six animals. Two clinical cases of cataract were included under the study as Group C. In all the animals of Group A and B, cataract was experimentally induced by injection of 0.5 ml of a 25% solution of calcium borogluconate into the anterior chamber of the eye, in strict aseptic conditionsand under general anaesthesia. In group A. cataract was treated by cryo-coagulation and in Groups Band C intra-capsular cryo-extraction of the cataractous lens was performed after pre-medicating and anaesthetising the animals. Surgery for cryo-coagulation (Group A) or intra-capsular cryo-extraction (Groups B and C) was performed under general anaesthesia with thiopentone sodium after premedication with xylazinc hydrochloride. The anaesthesia was found to be satisfactory at the time of induction and during cataract surgery. Extensive lateral canthotomy was found to be necessary in all the animals at surgery in order to ensure adequate exposure of the globe. Rectal temperature and respiration rate dropped slightly post-operatively, but returned to normal values by the second day after surgery. Pulse rate dropped markedly following surgery, but returned to normal by the sixth day after surgery. Total leukocyte count increased slightly 24 hours after surgery. but then decreased and remained within normal ranges thereafter. There was increase in neutrophil. eosinophil and monocyte count had increased upto 24 hours after surgery. but was normal thereafter. Lymphocyte eount decreased upto 24 hours post-operatively. All the animals .remained in good condition throughout the observation period, except for one animal. Conjunctivitis persisted only in one upto the 11 th day. Corneal oedema persisted throughout the period of observation in four animals. One animal had complete corneal clarity by day seven. In the other animal the cornea cleared on day 23. Uveitis persisted for varymg periods in the animals Photophobia and blepharospasm resolved by day six in all animals. One animal had no posterior or anterior synechiae following surgery and its vitreous body was clear, allowing easy exam ination of the retina. Aqueous flare, indicative of increased protein in the aqueous humour, could not be determined in any animal. Functional vision was not returned in any animal except A4. In Group B, vitreous prolapse occurred during surgical removal of the lens by intra-capsular cryo-extraction. This prolapsed vitreous was excised and did not cause complications. Rectal temperature increased slightly 24 hours after surgery, but had returned to normal values by the 15th post-operative day. Pulse rate decreased slightly 24 hours after surgery, but attained normal values by the second post-operative day and stayed so thereafter. Respiration rate decreased markedly for 24 hours following surgery, but reached normal values two days post-operatively. There was no change in the colour of the mucous membrane of the contralateral eye (used as control) at any time during the period of : observation. The total leukocyte count increased slightly following surgery and continued to be so till 24 hours after surgery and thereafter it decreased and was within normal limits thereafter. The neutrophil count increased till 24 hours post-operatively and returned to normal range thereafter. Lymphocyte count decreased slightly 24 hours after surgery, but returned to normal range by the i s" post-operative day. Eosinophil count increased 24 hours after surgery, but had reached normal values by the i s" post-operative day. Monocyte count became zero after surgery, but then increased slightly and continued so thereafter. All the animals remained in good general condition until the end of the observation period, with no evidence of infection in the operated eye. Intra-ocular pressure decreased slightly following surgery, but had returned to normal ranges by the end of the observation period. Animals B 1, B2 and B4 had persistent conjunctivitis and corneal oedema throughout the period of observation and were unable to negotiate an obstacle course or locate and track mobile or stationary objects even in conditions ofbright ambient light. All other animals in this group were able to perform satisfactorily in the tests of visual function by the end of the observation period. In Group C, treatment of cataract was by intra-capsular cryo- extraction, as in Group B. The results obtained were similar to those for Group B. Animal C2, however. took 52 hours to recover from anaesthesia and died on the sixth day following surgery. The death could not be attributed to complications of cataract surgery. Rectal temperature decreased slightly following surgery, and then increased slightly but returned to normal values at the end of the observation period. Pulse rate decreased immediately after surgery and then returned to normal ranges by the eighth day after surgery. Respiration rate decreased markedly immediately after surgery. but returned to the normal range within 24 hours after surgery. Colour of mucous membrane of the contralateral eye did not show any change at any time during the period of observation. Total leukocytc count increased slightly upto 24 hours after surgery but returned to normal ranges thereafter. The neutrophil and eosinophil and monocyte counts increased after surgery but returned to normal ranges thereafter. The lymphocyte count was markedly decreased at 24 hours after surgery, but then returned to normal ranges thereafter. The surviving animal in Group C showed low grade corneal oedema until day 31 following surgery, but it had blink reflexes and the iris was visible. Conjunctivitis had cleared by day six following surgery. The animals were monitored for visual capability following surgery. The tests were conducted by evaluating the animals' ability to negotiate an obstacle course under photopic and scotopic light conditions, after blind folding the left eye with an eye shield. The animals were also tested for their ability to locate a stationary object and to track a moving object under varying conditions of ambient lighting. Tests of ocular functional integrity were conducted by evaluating menace and photomotor pupillary reflexes. Animal Cl was able to locate or track stationary objects In dim light. It could track moving ohjects in all light conditions. From the results obtained in thc present study. it was found that only one out of the six animals showed restoration of functional vision following treatment of cataract by cryo-coagulation of the lens. In the case of the treatment by intra-capsular cryo-extraction. four out of eight animals showed restoration of functional vision thus showing a success rate of 50% for intra-capsular cryo-extraction of cataract. From the results it can be concluded that: 2. Cataract could be effectively induced using 0.5 ml of calcium borogluconate solution (25%) injected into the anterior chamber of the eye. 3. Pre-medication USing xylazine hydrochloride followed by general anaesthesia USing thiopentone sodium IV was satisfactory for the induction of cataract and the treatment of the cataractous lens. 4. Intra-capsular cryo-extraction IS a better method In treating cataractous lenses in dogs.
Open Access
Comparison of crushing and modified gambee techniques for intestinal anastomosis in dogs under xylazine anaesthesia
(Department of Surgery, College of Veterinary and Animal Science, Mannuthy, 1995) Venkatesan, C; KAU; Abraham Varkey, C
The study was conducted on 12 apparently healthy, adult mongrel dogs of either sex, dived in to two groups viz, group I and group II, each consisting of 6 dogs. All the animals were pre-mediacated with diazepam at the rate of 1 mg/kg bodyweight intravenous and anaesthetized with intramascular administration of xylazine hydrochloride at the rate of 2 mg/kg bodyweight. In the animals of group I and to end intestinal anaestomosis was performed with crushing pattern of suturing and in the animals of group II end to end intestinal anastomosis was performed with modified Gambee pattern of suturing. The anesthetic technique was satisfactory for the surgical procedure in all the animals . The induction tome for anaesthesia was 15.50 + 1.00 minutes. THe anaesthetic effect persisted for about 48.25-1.10 minutes. The abdominal muscle relaxation and analgesic effect were found satisfactory. The recovery period was 30.00 + 1.50 minutes and was smooth and uneventful. The time required to perform anastomosis using crushing and modified gambee techniques were 26.30 + 1.40 and 35.70 + 0.87 respectively. The average number of sutures used in both techniques were 15. In both groups, animals well tolerated food in the immediate post-operative days. However, one animal in group I and three animals from group II, had vomition immediately after consumption of milk at 12 hours post – operatively. Physiological and haemotological parameters did not reveal any significant variation. In both the groups , post operative lateral and ventrodorsal radiographs showed free passage of contrast medium (Barium Sulphate) upto the terminal colon. There was no evidence of anastomotic leakage and proximal distension in both the groups. Autopsy findings revealed that all the animals had adhesion at the anastomotic site with omentum. Adhesion to adjacent intestinal serosal surface were observed in one animal in each group . Gross evaluation of the adominal cavity revealed a low-grade peritonitis in one animal of group I on 3rd post surgical day. The Luminal stenosis of the anastomotic site revealed, maximum luminal stenosis (47.83%) at 7th post –operative days and minimum (21.74%) at 28th day following crushing pattern. In modified Gambee pattern, the maximum luminal stenosis (42.85%) was noticed at 5th and 7th post –operative days and minimum stenosis (7.32%) at 28th day. The luminal stenosis did not produce any clinical symptom in any of the dogs in both the groups. Angiograms of early post – operative day (5th day) showed diffused, avascular zone at the anastomotic site in both the techniques. Eventhourgh, there was commencement of proliferation of vessels and its invasion into anastomotic site by 14th day in the crushing anastomosis, normal vascularity and its crossing over could not be observed till 28th day, where as the modified gambee anastomosis showed proliferation and crossing over of fine arterioles by 28th day. Histological examination demonstrated that in crushing anastomosis comparatively moderate inflammatory reaction with predominance of mononuclears and few polymorphs on the 3rd day, but from 14th day there was only mild inflammatory reaction which which persisted upto 28th day. In modified Gambee technique, only mild reaction was seen on the early post opestive days and it subsided by 21st day. Rapid regeneration of epithelium was noticed in the modified Gambee (7th day) than the crushing technique. On the 28th day, in both the techniques all layers appeared in good apposition with proliferation of connective tissue on the muscular, subserosal and submucosal layers.
Open Access
Processed aortic allografts for oesophagoplasty in dogs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1998) Balagopalan, T P; KAU; Muraleedharan Nayar, K N
The study was conducted with the objectives of a. Preparation, preservation and evaluation of chrome/ glutaraldehyde cross linked aortic tissue of dogs and to compare the relative acceptability and efficacy of them for cervical oesophagoplasty in dogs, and b. Evaluation of modified pharyngostomy feeding method and its effect on healing at cervical oesophagoplasty site in dogs. The experiment was conducted in thirty, apparently healthy, adult, nondescript dogs of either sex weighing 9-13 kg. The animals were randomly divided into three groups viz.,, group I, II and III. Group II and III were subdivided into two subgroups each, namely IIA, IIB and iilA, IIIB. Group I and the subgroups consisted of six animals each. Animals of group I were subjected to sham operation. Cervical oesophagoplasty using chrome processed aortic allograft and glutaraldehyde processed aortic allograft were performed in animals of group II and III respectively. Pharyngostomy tube feeding was instituted in three animals of group I and all animals of subgroups IIB and IIIB. Tissue samples of thoracic aorta harvested from dogs, processed and crosslinked with chromic sulfate and glutaraldehyde were used as graft materials. Chrome processed aorta showed better biomechanical qualities except for tensile strength than glutaraldehyde processed aorta. The grafts had fairly good handling qualities and shelf life. All the animals were premedicated with triflupromazine hydrochloride and anaesthetized using thiopentone sodium to effect. Wound, oval in shape measuring 3-4 cm long and l/3rd of the circumference of the oesophagus was created in all experimental animals. Oesophagoplasty was performed by fixing the graft material over the defect using 5-0 braided silk thread and continuous lock stitch sutures. Left side' pharyngostomy was performed in 15 dogs. Suitably designed siliconised catheter made up of modified polyvinyl chloride with an attached X-ray opaque line was used as pharyngostomy tube. The tube was kept in situ for a period of 15 days postoperatively for administration of fluid diet. The animals were kept under observation for varying periods of 15, 30 and 60 days postoperatively. The animals of subgroups IIB and IIIB became alert and active earlier than that of IIA and IIIA. At the cervical region, the operated site showed mild inflammatory reaction by 1-2 days postoperatively in all animals. The sutures were removed after normal healing by 7-8th day in all animals except one each in subgroup IIA and IIIB, where it was removed on the 10th day. Mild bleeding while performing pharyngostomy (one dog) and moderate pain and slight swelling around the pharyngostomy tube entrance site (3 dogs) were observed in subgroup IIB. One animal in subgroup IIIB showed severe inflammatory oedema around the tube entrance site. Tolerance of pharyngostomy tube was excellent in 13 dogs. The pharyngostomy wound healed completely by 14-15th day after removal of the tube in all the dogs. All the animals started feeding on liquid food by seventh day postoperatively in subgroups IIA and IIIA following hyperalimentation via intravenous route and by 15th day in subgroups IIB and IIIB following hyperalimentation via pharyngostomy tube. They maintained normal apetite and feeding habits thereafter during the period of observation. Mild swelling at the operated site while swallowing (3 dogs) and vomiting (one dog) were noticed among the animals of subgroup IIA and IIIA. Initial tube obstruction during first feeding (6 dogs), vomiting after first feeding (2 dogs) and mild diarrhoea (one dog) were observed among animals of subgroup IIB and IIIB.
Open Access
Treatment of fracture of metacarpus in calves using autogenous rib graft
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 1994) Syam, K Venugopal; KAU; Jalaluddin
The present study was conducted on 12 apparently healthy, crossbred male calves six to twelve months of age and weighing 50 to 80 kg, divided into two groups of six animals each (Group A and B). A transversa mid shaft fracture was created on the right metacarpus by open method under sedation with Triflupromazine hydrochloride at the rate of 0.25 mg/kg body weight IM and diazepam at the rate of 0.20 mg/kg body weight IV followed by local infiltration analgesia using two percent solution of lignocaine hydrochloride. In group A, the fracture was reduced and the fragments were retained in position by placing two freshly cropped autogenous rib grafts subperiosteally, one on the anterior aspect and the other on the posterior aspect of the metacarpus. The grafts were fixed in position by hemicerclage wiring using stainless steel wires at two places. The limb was immobilized with four cotton padded bamboo splints and plaster of paris cast. In group B, the fracture was reduced and the wounds were sutured. The limb was immobilised with four cotton padded bamboo splints and plaster of paris cast. A sham operation was performed on the left metacarpal region on the same day by incising the skin upto the periosteum and suturing it. By the end of the second week, all the animals could get up and lie down without assistance. Four animals of Group A and three animals of Group B started bearing weight on the fractured limb from varying periods. Favouring of the fractured limb, limping and nodding of the head were observed in all the animals. Pawing action with the fractured limb and stumbling were observed in two animals each from both the groups. Dragging of the toes was observed only in one animal, in group B. Infection and suppuration at the suture line was observed in one animal of group A. Plaster of paris cast remained intact throughout the period of observation in all the animals. One animal from group A and four animals from group B required reinforcement of plaster cast. Marked displacement of the distal fragment was noticed in one animal each in Group A and B. But deviation of the distal fragment at the fracture site was observed in one animal of Group A and four animals of Group B. Grafts were in position in all the animals throughout the the period of observation. They became radiographically indistinguishable from fourth week onwards. Radiographically visible callus was found by the third week in group A and by the first week in group B. Partial obliteration of the fracture gap was observed by the end of fourth week in both the groups. Fractured bone cropped after two weeks revealed mobility between the fragments in both the groups. The bones cropped after four weeks showed well developed callus uniting the fragments and there was no mobility at the fracture site in both the groups. The callus developed at the end of six weeks and four weeks were grossly similar in both the groups. Histological examination of the callus cropped at second week in group A revealed periosteal and capillary proliferation along with new trabecular bone formation around the graft site. In group B, fibrous tissue proliferation exceeded trabecular new bone formation. The callus cropped at fourth week in group A revealed extensive areas of graft vascularisation and zones of new bone formation. In group B, cartilage formation was seen along with zones of new bone formation and fibrous tissue proliferation. Replacement of the graft tissue with proliferating blood vessels, osseous tissue and connective tissue were observed in the callus cropped from the animals of group A, at the end of six weeks. In group B, well developed internal callus, extensive periosteal callus, proliferation of fibrous tissue and trabecular ossification centres were observed. Fibrocartilage was noticed in the callus in one animal of group B.
Open Access
General anasthesia in dogs with Tiletamine-Zolazepam
(Department of Surgery, College of Veterinary and Animal Science, Mannuthy, 1995) Rajankutty, K; KAU; Muraleedharan, Nair K N
The present study was undertaken to findout the efficacy of tiletamine – zolazepam alone and with xylazine premedication for anaesthesia in dogs and to evaluate the systemic changes consequent on the administration of these drugs. The experimental study was conducted on 36 adult non- descript dogs of either sex. The animals were randomly divided into two groups, (Group I and II ) consisting of 18 animals each. Each group was further divided into three subgroups, viz A, B and C, consisting of six animals each. Atopine sulphate (0.04 mg/kg bodyweight) was administered IM 15 minutes prior to the administration of the experimental drugs in all the dogs. Tiletamine – zolazepam (T-Z) combination was administered IM at the rate of 5mg, 10 mg and 15 mg/kg bodyweight in the subgroups IA, IB and IC respectively. Xylazine at the rate of 0.5 mg/kg bodyweight and 15 later, T-Z combination at the rate of 5 mg, 10 mg and 15mg/kg bodyweight were administered IM in the subgroups IIA , IIB and IIC respectively. The induction time was 6.17 + 1.01 min, 4.33 + 0.21 min and 4.33 + 0.49 min in subgroups IA, IB and IC respectively, and 3.33 + 0.62 min, 3.17 + 0.48 min and 2.83 + 0.54 min in subgroups IIA, IIB and IIC respectively. Increase in the dose of T-Z reduced the induction time and premedication with xylazine further reduced the induction time and induction was smooth. The onset of effect of tiletamine – zolazepm was characterized by the winking of eyes, yawning, licking and protrusion of tongue. The eyes remained open and pupils were slightly dilated. The palpebral and pedal reflexes and swallowing movements were not abolished. Salivation was scanty in both the groups. In the animals of group II the eyes were partially closed and palpebral and pedal reflexes were abolished but the swallowing movements were not. Protrusion of tongue, though present, was not to the extent that was observed in the animals of group I. Rhythmic side to side head movements were noticed during induction in all the animals of group I but not in animals of Group II. The duration of anaesthesia was 33.67 +5.88 min, 57.83 + 6.17 min, and 89.00 +2.86 min in subgroups IA, IB and IC respectively, whereas it was for 49.67 + 6.643 min, 105.17 + 10.31 min AND 125.83 + 10.78 min in subgroups IIA, IIB and IIC respectively. An increase in the dose of tiletamine – zolazepam had prolonged the duration of anaesthesia and premedication with xylazine produced still longer duration of anaesthesia. The jaw musculature maintained the tonus with the lower doses of tiletamine – zolazepm, but at 15 mg/kg bodyweight, the jaw muscles, though not fully relaxed, permitted endotracheal intubation. Administration of tiletamine – zolazepam with xylazine resulted in relaxation of the jaw muscles and permitted endotracheal intubation. Relaxation of the abdominal muscles was moderate to good when tiletamine – zolazepam alone was administered, whereas it was excellent with xylazine premedication. Administration of tiletamine – zolazepam alone was found insufficient for carrying out surgical procedures, but with xylazine premedication muscle relaxation and analgesia was satisfactory. The recovery time was 111.50 + 14.53 min, 116.50 + 10.46 min and 180.33 + 10.57 min in subgroups IA,IB and IC respectively, and it was 160.00 + 17.70 min, 180.00 + 14.94 min and 181.06 + 12.82 min in subgroups IIA, IIB and IIC respectively. Increase in the dose of tiletamine – zolazepam had delayed recovery and xylazine premedication delayed it still further. During recovery, paddling and vocalization were common in dogs of group I but not in dogs of group II. Reduction in rectal temperature was observed only in animals of subgroups IB and IC and in all the animals of Group II. Marked increase in pulse rate was observed in group I than in Group II. Respiration rate was decreased in both the groups. A mild increase in diastolic pressure was observed in Group I. The systolic and diastolic pressure were seen decreased in Group II. The changes in the coagulation time of blood was within the normal limits in both the groups. Increase in heart rate with depression of T-wave, biphasic T-wave and spiking of T-wave were the changes in electrocardiogram. But the changes were corrected spontaneously. There was slight decrease in the erythrocyte sedimentation rate in animals of group I but there was no change in Group II. Reduction in the packed cell volume was observed in both the groups, but it was more, after xylazine premedication. Slight decrease in haemoglobin concentration was noticed in subgroup IC. But there was no change in Group II. Decrease in total erythrocyte count was noticed in both the group and the decrease was more when premedicated with xylazine. The total leukocyte count was seen increased in subgroup IA and IB but it was seen decreased in subgroup IC, but there was no change in group II. Decrease in lymphocyte count with increase in neutrophil count was observed in subgroups IA and IIA. In subgroups IB and IC there were increase in lymphocyte count with decrease in neutrophil count, but in subgroups IIB and IIC a decrease or no alteration in the count of lymphocytes was observed. Monocyte and eosinophil counts were increased and the basophil count remained insignificant. There was marked increase in the serum glucose value following the the administration of tiletamine – zolazepam and with xylazine premedication the increase was more. No significant change was observed in the serum electrolytes (Na+, K+, and Cl-), total serum protein content and serum urea nitrogen value in both the groups. Slight increase in serum aspartate aminotransferase (AST) and serum alanine aminotransferase (ALT) values were observed in Group I,and in Group II there was increase in AST value, but the ALT value decreased initially and was followed by an increase. Histopathological examination of liver revealed cloudy swelling and mild fatty changes and kidney revealed cystic dilation of the renal tubules along with focal areas of nephrosis.
Open Access
Comparative study of healing of fracture of metacarpus in calves using different external immobilisation methods
(Department of Surgery, College of Veterinary and Animal Science, Mannuthy, 1990) Kalyani, Biswas; KAU; Muraleedharan, Nayar K N
The study was conducted on 18 crossbred male calves divided in three groups of six animals each. Under local anaesthesia (ring block) fracture was created at the midshaft region of the large metacarpal and was reduced. The limb was immobilized with (i) plaster of paris cast (Group A), (ii) transfixation apparatus and plaster of paris cast (group B) and (iii) cast using freshly prepared gum and bandgage (group C). The animals were kept under observation upto six weeks. One animal from each group was sacrificed at the second week and fourth week, and the remaining animals at the sixth week. Oedema was observed distal to the cast in two animals each in Group A and C and four animals in Group B. Pointing of the toes was noticed in three animals of group A, two animals of group B and one animal of Group C. Dragging of the toes was noticed in three animals each of group A and C. All the animals, except one from Group A, were bearing weight on the limb and could get up and lie down unassisted by the first week. Four animals of group A, two animals of group B and three animals of Group C favoured the limb throughout the period. In group A and B, the plaster cast remained intact throughout the period of observation in one animal, had to be changed once in three animals and twice in two animals. In group C, the cast remained intact throughout the period of observation in three animals and was changed once in three animals. Radiodense area was observed at the fracture site in one animal by the first week, in four animals of each group by the second week and in all animals except one by the third week and in all the animals by the fourth week. The fracture line had become less district in one animal of group A, two animals of Group B and one animal of group C by the third week. Ossification of the callus was noticed in all the animals by the fourth week. The fracture line was less distinct in all the animals of Group A, three animals of group B and two animals of Group C. Ossification of the callus and its union was noticed by the fifth week in all the animals except one animal of Group C. However, in group B, ossification was more extensive. Partial obliteration of the fracture line was seen in all except three animals. Extensive ossification of the callus with continuity between the callus of the two fragments and partial obliteration of the fracture line was observed in all the animals, except one animal of Group C by sixth week. The fractured bone cropped after two weeks revealed that in Group A, the fragments were separate and there was mobility at the fracture site but in Group B and C , mobility was less. Soft callus between the fragments and restricted mobility at the fracture site was noticed in the bones cropped at four weeks in animals of all the groups. The callus was well developed and the fragments were firmly fixed by the callus in the bones cropped at six weeks, I all animals of groups A and B and in three animals of Group C. Microscopic examination of the callus at two weeks revealed periosteal and capillary proliferation with zones of necrosis of cortical bone at the fracture site in Group A. In group B and C, the proliferative changes were more marked along with zones of ossification. The callus cropped at four weeks, revealed marked connective tissue and capillary proliferation along with zones of ossification and formation of trabeculae in Group A and C, and in Group B ossification was more extensive. Extensive ossification and interconnected trabeculae continous with the cortical bone was observed in the callus cropped at six weeks in all the three groups. Zones of hyaline cartilage were observed in the callus in the animals of Group A and one animal of Group B and Zone of suppuration in one animal of Group C. Suppuration at the suture line involving the callus and fracture site was observed in one animal of Group C. Partial displacement of the fragments after immobilization was noticed in two animals of group A, three animals of Group B and four animals of Group C.
Open Access
Ascending coccygeal venography in evaluation of paraplega in dogs
(Department of Surgery, College of Veterinary and Animal Sciences, Mannuthy, 2002) Joshi George; KAU; Sarada Amma, T
The study was undertaken with an objective of evaluating the comparative efficacy of ascending coccygeal venography and epidurography in locating the site and type oflesion in dogs suffering from paraplegia. The study was conducted in 12 clinical cases presented at the Surgery unit of Veterinary College Hospital, Mannuthy and were designated into two groups (Group A and B) of 6 animals each. Survey radiographs were taken in all the animals prior to the contrast radiographic procedures. Iohexol (300 mgIfrnl) was used as the contrast medium at a dose rate of 80 mgIlkg body weight for epidurography and 100 mgIlkg body weight for ascending coccygeal venography. In Group A, epidurography was conducted by introducing the contrast medium through the lumbosacral junction into the epidural space. The contrast medium was given as slow infusion and the zero minute radiograph was taken, as the last milliliter of the contrast medium remained to be injected. Subsequent radiographs were taken at the 3rd and the 5th minute. In Group B, ascending coccygeal venography was conducted by introducing the contrast medium intravenously as slow infusion into the superficial lateral coccygeal vein of anyone side near the base of the tail, after sufficient abdominal compression. Lateral/ventrodorsal radiographs were taken, as the last milliliter of the contrast remained to be injected. Subsequent radiographs were taken at the 3rd and the Sth minute. All the animals were subjected to a thorough clinical and neurological examination prior to radiography. Physiological and haematological parameters were evaluatedjust before and 24 hours after radiography. All the animals were observed for one week for the presence of any side effects/complications. The physiological and haematological parameters were within the normal range in all the animals both before and 24 hours after radiography. Iohexol was found safe for neuroradiological studies as none of the animals exhibited any side effects/complications during the period of observation for one week. In Group A, epidurography produced good quality radiographs and was consistent in all the six cases. Abnormalities/lesions could be located in four cases. The remaining two cases showed normal epidurographic pattern and could not be correlated with the neurological examination. In Group B, successful venograms were obtained in three cases in which lesions were located and correlated with the neurological examination. In the remaining three cases, the venous sinuses could not be opacified and hence failed in identification oflesion. The changes identifiable as lesions in Group A were attenuation of the contrast col W11l1 , considerable narrowing and elevation of the column, and failure of the opacification ofa specific region of the column. The changes identifiable with a lesion in venography were abrupt attenuation of the venous sinuses, considerable elevation of the venous sinuses and progressive thinning and attenuation of the veins. Thus epidurography showed consistency III all the SIX cases and diagnosis could be made in four out of six cases whereas diagnostic venograms were obtained only in three out of six cases. Thus ascending coccygeal venography was found inconsistent and required further studies before recommending this as a routine practice. Though epidurography possessed a distinct advan tage over ascending coccygeal venography in consistency and technical and diagnostic feasibility, epidurography couldnot be advocated as a sole diagnostic procedure and should be used as an adjunct with other .diagnostic aids after proper clinical and neurological examination.

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