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Subject: Veterinary Public Health × Author: Bindu Raj, R × Author: KAU × End date: 2002 × Start date: 2002 × Thesis Type: M.V.Sc. ×
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    ThesisItemOpen Access
    Bacteria of public health significance in broiler dressed chicken
    (Department of Veterinary Public Health, College of Veterinary and Animal Sciences, Mannuthy, 2002) Bindu Raj, R; KAU; Nanu, E
    In the present investigation, 60 chicken carcasses, consisting of 20 each, collected from retail shops selling dressed birds which were brought from outside the state (A), shops selling locally reared dressed birds (B) and dressed chicken from the Kerala Agricultural University Poultry Farm (C). The bacterial quality of each carcass was evaluated by estimating the total viable count (TVC), coliforms count (CC), Escherichia coli count (ECC) and faecal streptococcal count (FSC). All samples were also tested for the presence of bacterial pathogens such as Escherichia coli, salmonellae, Staphylococcus aureus and Listeria monocytogenes. Analysis of variance test of the mean TVC revealed significant (P<0.05) difference between the count of the samples from source Band C. The overall mean TVC of the samples was 7.89 ± 0.07 10glO cfulml of the carcass rinse. The samples from source B had the highest mean TVC (8.06 ± 0.11 log., cfulml) and lowest mean count was seen in the samples belonging to the source C (7.69 ± 0.13 log., cfulml). The samples from source A had a mean TVC of7.94 ± 0.08 log., cfulml. The count at the level of 109 cfulml was observed in one of the samples belonging to the source B. The count in three (5%), 34 (56.67%) and 22 (36.67%) samples was at the level of 106, 107 and 108 cfulml, respectively. Analysis of variance test showed significant (P<0.05) difference between the mean CC of the samples from source A and C and Band C. The overall mean CC of the carcass rinse was 4.97 ± 0.1 0 log., cfulml. The samples from the source A had the highest mean count (5.30 ± 0.10 log}o cfulml) and lowest in the samples from source C (4.37 ± 0.17 log., cfulml). The mean CC of samples from source B was 5.23 ± 0.13 log}o cfulml. The count in l.67 per cent and five per cent of the samples was at the level of 102 and 106 cfulml, respectively. The count in 1l.67, 3l.67 and 50 per cent samples was at the level of 103, 104 and 105 cfulml, respectively. Analysis of variance test revealed significant (P<0.05) difference between mean ECC of the samples belonging to the source A and C and Band C. The overall mean ECC of samples from the three sources was 2.20 ± 0.27 log}o cfulml. The mean count of the samples belonging to the source A, Band C was 2.83 ± 0.48, 2.66 ± 0.46, 1.08 ± 0.35 10glO cfulml, respectively. The count in five, 20 and 26.67 per cent of the carcasses was at the level of 102, 103 and 104 cfulml, respectively. Analysis of variance test revealed highly significant (Pdifference between FSC of samples from source A and B, A and C and B and C. The overall mean FSC of the samples was 4.32 ± 0.09 log., cfulml. The highest mean count was observed in samples from source A (4.99 ± 0.10 log., cfulml) and the lowest mean count in samples from source C (3.75 ± 0.l0 10glO cfulml). The mean FSC in samples from source B was 4.20 ± 0.10 10glO cfulml. The count in 38.33, 46.67 and 15 per cent samples were at the level of 103, 104 and 105 cfu/ml, respectively. Correlation coefficient test of the data revealed significant (P<0.05) association between the mean CC and FSC. A positive and non- significant correlation existed between mean TVC and CC, TVC and ECC, TVC and FSC, CC and ECC and ECC and FSC. Escherichia coli was isolated from 51.67% of the samples. The 44 E. coli isolated from the samples were serotyped and fell into 21 serotypes, six rough strains and two .untypables. The serotypes consisted of 05,08, 014, 025,033,041,049,066,078,081,084,085,091,0116, 0121, 0131, 0132, 0146, 0150, 0157 and 0161. Salmonellae were isolated from two samples. Staphylococci were isolated from 30% of the samples, but none of them were coagulase positive. All samples were found free from Listeria monocytogenes.