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ThesisItem Open Access Hypolipidaemic effect of allium sativum and emblica officinals in rabbits(Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, 1992) Mini, K P; KAU; Gopakumar, NThe study was undertaken with the objective of determining the hypolipidaemic effect of Allium sativum and Emblica officinalis in hyper lipidaemic rabbits. The different forms of the indigenous agents tried were aqueous extract of A. sativum and fruit pulp as well as aqueous extract of E. officinalis. The effects produced by the above agents were compared with that of the known hypolipidaemic drug namely, Gemfibrozil which served as the positive control drug. Thirty Newzealand white adult male rabbits were used for the study. The average body weight of the animals were 1.5 kg. They were housed in cages designed for the purpose. Each rabbit received 200 g standard rabbit’s feed per day. The experiment was conducted in two stages. The first step was to render all the rabbits hyper chloesterolaemic. In order to achieve this goal, the standard ration was supplemented daily with 100 mg cholesterol and 8 g Vanaspathi for each rabbit. The rabbits were fed on this diet continuously for 60 days when they developed hypercholesterolaemia. This was ascertained by the estimation carried out initially and every fortnightly during this period. The parameters estimated were plasma cholesterol and plasma triglyceride. The second part of the study was to evaluate the hypolipidaemic efficacy of the chosen indigenous preparations in comparison to that of Gemfibrozil. Each agent was tried on a separate group by dividing the hypercholesterolaemic rabbits into five groups of six each. Group I was kept as the control group which received no treatment. Group II was administered aqueous extract of A. sativum 10 ml (2g/kg b.wt.) orally. The animals of Group III were treated with fruit pulp of E. officinalis at a dose rate of lg/kg orally. Group IV animals received aqueous extract of E. officinalis, orally at a dose rate of I g/kg prepared in 10 ml of water. The Group V served as the positive control which received Gemfibrozil at a dose rate of 120 mg/kg orally. All the drugs were administered for a period of 75 days. The high cholesterol containing diet was continued throughout the period of study. The difference brought about by the above agents on plasma cholesterol and triglyceride of hyperlipidaemic rabbits was determined by routine estimations of the above parameters carried out every fortnightly. At the end of the period of study histopathological studies of liver and aorta were also performed in order to detect the structural changes in tissues caused by the different treatments. The control group of animals increased their plasma cholesterol and triglycerids by 76.59 and 81.36% respectively. This increase was found to be statistically significant. The liver and aorta of these animals also supported the above finding on histopathological examination. Diffused fatty changes was noticed throughout the section of liver and aorta. The hepatocytes were filled with fat and the nucleus was displaced. Lining of the wall of aorta also showed severe fatty infiltration in the control group. It was found that administration of aqueous extract of A. sativum reduced plasma cholesterol by 79.08 per cent and plasma triglycerides by 75.76 per cent within a period of 75 days. Both the reductions were highly significant. Compared to the control group, the histopathological findings of this group showed that garlic was very effective in counteracting the fatty changes induced by high cholesterol diet in rabbits. The fatty changes of liver was only of a mild degree and the fatty infiltration of aorta was also very mild. The percentage reduction obtained in the case of fruit pulp of E. officinalis was 69.74 and 78.20 with respect to cholesterol and triglycerides which was also statistically significant. Aqueous extract of E. officinalis administered to the fourth group of animals could produce a reduction in plasma cholesterol and triglyceride by 79.40 per cent and 82.80 per cent respectively. The histopathological studies conducted in the above two groups showed almost similar findings. Compared to the control group, the degree of fatty changes was only mild to moderate. Infiltration of fat into the elastic tissues of aorta was also very mild. Hence it can be suggested that E. officinalis is capable of counteracting the fatty changes in liver and aorta partially. Gemfibrozil which served as the positive control brought about a percentage reduction of 78.43 with respect to cholesterol and 81.54 per cent in the case of triglyceride, both were found to be highly significant. The above observation was well supported by the photomicrographs of liver and aorta taken from the animals of this group. Mild to moderate degree of fatty changes was noticed in the liver and aorta. Aorta did not reveal prominent changes like that of the control group due to fatty infiltration. The histopathological observation made in the case of Gemfibrozil resemble that of E. officinalis. From the results of the present study, it can be inferred that aqueous extract of A. sativum and fruit pulp as well as aqueous extract of E. officinalis are effective as hypolipidaemic agents and this finding is further asserted by the simultaneous histopathological studies carried out. Both the indigenous agents were capable of correcting the fatty changes produced by the fat containing diet to a considerable extent and garlic was found to be superior to E. officinalis in this respect. The efficacy of these agents in lowering plasma cholesterol and triglyceride was comparable to that of Gemfibrozil as shown by the statistical analysis of the results obtained. Hence these agents prove to be of value as hypolipidaemic agents in the future clinical trials that can be carried out in animals and also in human beings.ThesisItem Open Access Assessment of the antipyretic and analgesic effect of selected indigenous plants in rats(Department of Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, 1992) Suresh, B; KAU; Marrykutty, PThe experiments were conducted in three different parts. In the first part of the experiment the antipyretic activity of Ocimum sanctum and Tinospora cordifolia was determined. Twenty four groups of ten albino rats each were used for the study. Pyrexia was induced by injecting 20 per cent yeast suspension subcutaneously. Benzene extract of O. sanctum was given at 50, 100, 200 and 400 mg/kg dose levels in four different groups. A dose dependent reduction in temperature was obtained after four hours of its administration. Four hundred mg/kg dose level produced an affective lowering in the temperature than other doses used and showed the reduction in the temperature from 38. 840 C to 37. 590 C . Dose rates used for essential oil of O. sanctum were same as benzene extract. Two hundred mg/kg dose level revealed a more effective reduction in the temperature than four hundred mg/kg. A reduction of temperature was observed from 39. 370 C to 37. 960 C after four hours of its administration. Single, double and triple the doses of decoction of O. sanctum were used for antipyretic study. Compared to other doses, double the dose of decoction produced a maximum reduction in the temperature. It produced a reduction from 38. 930C to 37.490 C. Dose rate used for benzene extract of T. cordifolia were 50, 100, 200 and 400 mg/kg body weight. A dose depandant reduction in the temperature could be observed after four hours of its administration. Four hundred mg/kg dose level revealed as an effective dose caused a reduction from 39.010 C to 37.770 C after four hours of its administration. Single, double and triple the dose of decoction of T. cordifolia were used for assessing antipyretic activity. Double the dose of decoction was found to be more effective. And it reduced the temperature from 39.720 C to 38.140 C after four hours of its administration. In the second part of the experiment analgesic effect of benzene extract of O. sanctum and T. cordifolia were evaluated. All the dose rates of both the drugs used were compared with aspirin for a period of two hours showed no significant analgesic effect. In the third part of the experiment long term effect of essential oil of O. sanctum and benzene extract of T. cordifolia were studied. Haematological parameters were determined at an interval of 15 days. Benzene extract of O. sanctum and T. Cordifolia produced a significant charge in the erythrocyte count from 45 days onwords. At the end of the study Benzene extract of T. cordifolia treated group revealed a significant change in the leucocyte count. Both the groups showed a significant change in the haemoglobin value from days onwards. Fifteen days after the administration of the drug, both the groups showed a significant increase in the neutrophil count and decrease in the lymphocyte count. At the end of study same effect was noticed. On 61st day histopathological studies of liver were conducted. Lesions observed in both the treated groups were in general, comparable with those of control group. It was found that neither essential oil of O. sanctum nor benzene extract of T. cordifolia caused lesions in hepatic tissue in any of the experimental animals.
