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Subject: Veterinary Pathology × Author: KAU × End date: 1994 × Type: Thesis ×
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    ThesisItemOpen Access
    Biology of neoplastic cells of ethmoid carcinoma
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1985) Madan Sing, Karki; KAU; Rajan, A
    Attempts were made to propagate the ethmoid carcinoma cells in vitro to study the biological behaviour of the neoplastic cells and their relationship with the host. Efforts were also made to transplant the neoplastic cells in laboratory animals and tumour bearing natural hosts so as to lay out a model system for further investigation. In vitro propagation was tried by obtaining the tumour tissues from nine cows and three goats. Cells were grown in Hank’s balanced salt solution (HBSS). TC – 199 and Dulbecco’s modified Eagle’s medium with 10 to 20 per cent calf serum. The cell suspension was seeded into tissue culture bottles, milk diluting bottles and test tubes and incubated at 370 C. No growth was observed in the cultures made from the tumour tissue of cows. The tumour cells from two goats grew in monolyerand were maintained for nine days. There was no difference in growth of cells in different media employed. Insulin supplementation in the media did not cause any difference in the growth of the cells. Subsequently the cells died even after subculturing and changing of the media. Absence of certain unknown factors required by the neoplastic cells for growth was considered responsible for failure of the growth. Transplantation studies were carried out in autologous, homologous and heterologous species of animals using the tumour tissue framgments, homogenate, filtrate and cell suspension. No autograft or heterotransplant were successful. Homologous transplantation resulted in a firm painless growth at the site of injection. The growth initiated a cell – mediated immune response at the site of implantation. Failure of neoplastic cells to grow in the other hosts was considered to be due to rejection of the transplants by the host’s immune system.
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    ThesisItemOpen Access
    Pathology of the reproductive organs in experimental hypothyroidism in goats
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1982) Nemali Mohan, Reddy; KAU; Rajan, A
    A survey study was conducted to assess the role of hypothyroidism in the etiology of reproductive disorders in goats. Lowered functional activity of the thyroid was associated with postpartum anoestrum, repeat breeding and delayed puberty. An experimental model of controlled hypothyroid state was induced in goats, using different dose regimes of thiourea with the objective of studying the sequence of physiopathological changes in hypothyroidism and its influence on reproduction. Clinically healthy young and adult cross-bred goats of both sexes were employed for the study. The animals were divided into control, Treatment I (T1) and Treatment II (T2) groups. Groups T1 and T2 were administered thiourea orally at the rate of 50 mg/kg and 100 mg/kg body weight respectively. Clinical symptoms and growth rate were studied. Protein bound iodine, total serum protein and serum cholesterol were estimated. Haematological data were collected and the semen characteristics were evaluated. On the 90th day of the experiment both control and experimental animals were sacrificed leaving at least one animal in each group. These animals were maintained without treatment with thiourea for a further period of 90 days and sacrificed. Cross lesions and histopathological changes in tissues were recorded. Weakness, lethargy, depression, reduced feed intake, subcutaneous oedema of varying degree, loss of libido in males and anoestrum in females were the important clinical features observed. There was stunting of growth and apparent reduction in weight of the animals dosed with thiourea. On discontinuation of treatment with thiourea all the clinical signs manifested disappeared and the animals gained weight progressively. There was significantly decrease in the protein bound iodine, and increase in the total serum protein and cholesterol level in all the animals dosed with thiourea. The values reached the normal level on discontinuation of treatment with thiourea. Microcytic hypochronic anaemia was evident in hypothyroid goats. There was improvement in haemogram values on withdrawal of thiourea administration. Reduction in quantity and quality of semen was observed in hypothyroid goats. The semen appeared normal in quality and quantity on discontinuation of thiourea administration. Gelatinisation of subcutaneous fat and hypertrophy and dilation of the left verntricle were the common findings at autopsy. There was significant increase in the relative weight of the thyroid, pituitary and the adrenal gland. Histologically the thyroid glands exhibited varying degree of hyperplasia and hypertrophy. Predominant histological changes in the pituitary was hyperplasia and hypertrophy of basophil cells and degenerative changes in the acidophils. Adrenal gland showed hypertrophy and depletion of fat in the zona fasciculata. On discontinuation of treatment with thiourea all the lesions disappeared. A significant decrease in the relative weight of the testis and epididymis was observed in all the goats dosed with thiourea. Histologically the seminiferous tubules were small and lined by single layer of spermatogonial cells. Some tubules contained degenerated and desquamated cells without any evidence of sperms. The tubules of the epididymis were small in size and the lining cells showed deciliation and desquamation. These degenerative changes were found to be reversible on discontinuation of treatment with thiourea. The accessory sexual glands, the seminal vesioles and the prostate weighed less in hypothyroid goats. Histologically the cells lining the glands were small inactive, vaculated and hyalinised. After discontinuation of administration of thiourea the reparative changes were almost normal. There was significant decrease in the relative weight of the ovary and uterus in all the animals dosed with thiourea. Histologically ovaries were found to be inactive and only few scattered immature follicles were seen. Uterine glands were few and non secretary type. The lining epithelial cells did not show active mitosis. On discontinuation of treatment with thiourea the weight of the ovary and uterus increased and histologically ovaries and uterus were found to be normal. Kids and female goats were more susceptible to the effects of hypothyroidism. The significant pathological changes observed in the reproductive organs have clarified the importance of hypothyroidism in inducing sub-fertility and infertility in goats. The reversible nature of the lesion was also proved.
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    ThesisItemOpen Access
    Pathology of the reproductive system in ducks
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1986) Jayakumar, P M; KAU; Valsala, K V
    A study was conducted on the pathology of the reproductive system in ducks. A survey conducted on the prevalence of diseases based on the data for a period of 11 years at the Government Duck Farm, Niranam revealed the incidence of hepatosis (64.58%), hepatitis (9.07%), enteritis (8.58%), tuberculosis (2.61%) and miscellaneous disorders (15.13%). The latter category included pulmonary edema, omphalitis and transport stress. In a separate study, the reproductive organs of 194 female ducks and 26 male ducks were examined systematically and gross and histopathological lesions encountered were studied. The diseases encountered in females were hypoplasia of left ovary and oviduct (20%), impaction of oviduct (5.45%), haemorrhagic cyst in the ovary (7.27%), salpingoperitonitis (10.9%), oophoritis (49.09%), cystic right oviduct (1.82%), mycotic salpingitis (1.82%) and tuberculous salpingitis (3.63%). In males atrophic testis (66.3%) and seminoma (33.3%) were the diseases recorded. Experimentally, pure aflatoxin B1 and ochratoxin A were administered to six ducks each, at the rate of 25 µg per duck daily for a period of three months. Clinically all the experimental ducks showed unthriftiness. But it was more pronounced in aflatoxin group. In both aflatoxin and ochratoxin group, the birds showed reduced weight gain and decreased feed intake. There was reduction in the weight of the testis in both aflatoxin and ochratoxin groups. Histologically, in the aflatoxin group, marked disruption in the organisation of germinal epithelium, absence of sperm bundles and edema of the interstitial tissue were the lesions observed. In the ochratoxin group, the basement membrane was found to be unaffected. Both aflatoxin and ochratoxin were found to cause degenerative changes in the testicular tissue. However, aflatoxin was found to cause more pronounced changes than ochratoxin. From the studies made, it was concluded that both aflatoxin and ochratoxin can induce degenerative changes in the testis and it was surmised that this would lead to subfertility and infertility. The need for feeding a diet free of mycotoxins to ensure profitable duck farming was stressed.
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    ThesisItemOpen Access
    Studies on pathological conditions in the mammary glands of cattle and goats
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1976) Lalitha Kunjamma, C R; KAU; Krishnan Nair, M
    An investigation was carried out to study the pathological lesions encountered in the mammary glands of cattle and goats from specimens selected from slaughter house and autopsy cases. The somatic cell count in milk from clinical and sub – clinical cases of mastitis was also undertaken. Two hundred quarters from cows and 67 halves from goats were subjected to detailed histopathological investigation. Mastitis was found to be the important type of lesion encountered. Lesions varied from subtle changes to intensive involvement with complete obliteration and atrophy. The lesions encountered were catarrhal mastitis and galactophoritis, suppurative mastitis, acute diffuse mastitis, necrotising mastitis, gangrenous mastitis, interstitial mastitis and tuber - culous mastitis. Other significant alternations associated with or without mastitis were congestion and oedema, squamous metaplasia of lining epithelium of ducts and cisterns and calcification of vessel walls. The calcification of the vessels was similar to that observed in ‘Monckeberg’s sclerosis’ in human beings. No tumours were found. It was suggested that lymphoid cells found in the interstitial septa of the mammary glands of goats affected with John’s disease might be the ‘T’ type of lymphocytes which are concerned with cellular immunity. The cystic changes encountered in the glands were not considered as the separate entity – Fibrocystic disease – but only compensatory processes as a result of fibrosis and atrophy of some part of the glandular and ductal system. Micro concretions were constantly observed and it was suggested that calcium got deposited over inspissated and hyalinised milk proteins. A total of 189 quarter samples of milk was also examined for total somatic cell count, differential count and by culturally for the presence of pathogenic bacteria and fungi. The total cell count in normal milk of cows and goats varied from 150,000 to 500,000 per ml. In sub – clinical and clinical cases upto 30,000,000 cells per ml could be counted. The percentage of neutrophils in normal milk was below 31% in goats and 43% in cows. The corresponding maximum values in mastitic cases were 88% and 92% respectively. In both cows and goats the chief etiological agent for mastitis as found in the present study was Staphylococcus. Other organisms encountered were Strephylococcus, Coliforms and Corynebacteria.
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    ThesisItemOpen Access
    Pathology of the eye in cattle bearing carcinoma of the mucosa of the ethmoid
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1982) Jose, C J; KAU; Ramachandran, K M
    Eighteen cattle from different parts of Kerala, affected with ethmoid carcinoma were subjected to detailed investigations of the ocular changes. The incidence of eye involvement in cases of ethmoid tumour in cattle was assessed on the basis of reports or informations received from field veterinarians and cattle owners from different dietricts of Kerala. Incidence, clinical manifestations, intraocular pressure, gross pathological cbanges and biometry of the eye were studied. The effect of the tumour mass on the eye and the possible causes for the different ocular changes has been discussed. The highest incidence was found in the age group of six to ten years. The earliest case was recorded in a heifer aged four years. The frequency of the incidence was high in females (88%). Crossbred animals were found to be more affected (83%). The tumour was also encounter~d in three purebred cattle. Ninetyfour percent of the tumour affected animals showed exophthalmos either unilateral (82%) or bilateral (18%). Tbe left eye was affected in 14 percent and the right eye in 86 percent cases. It is suggested that 37 exophthalmos can be considered as a pathognomonic clinical manifestation for ethmoid tumour affected cattle. Blindness was observed in 90 percent of the affected eyes, ectropion in 85 percent cases and corneal cbanges in the form of keratitis or opaCities in 90 percent cases. Episcleritis, anterior uveitis, euphora, purulent discharges were the other clinical manifestations. Glauc~a, was found to be a characteristic feature in ethmoid caroinoma. Involvement of the optic nerve, optic diso, retina, vitreous and other deep structures of the eyes were also notioed by gross examination of the whole eye at autopsy. The lens showed varying degrees of displacement or luxation.
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    ThesisItemOpen Access
    Ochratoxicosis in quails (Coturnix coturnix japonica) with special reference to Inununopathological response
    (Centre of Excellence in Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1992) Amir Abbas, Farshid; KAU; Rajan, A
    In this investigation the pathological effects of OA in quail embryo and adult quail were studied with special reference to the immune system. Ochratoxin A (OA) was inoculated into the quail embryos, at the dose rate of 0.02 ^g per embryo. There was 68.66 per cent mortality which clearly indicated the direct action of OA. The embryos which were sacrificed on the 13th and 16th day of incubation showed significant reduction in the body weight, weight of bursa of Fabricius, spleen and thymus. Histological changes in these lymphoid organs were, atrophy, necrosis and depletion of lymphoid elements. Ultrastructurally the changes were time dependent. There were changes in the shape of the nucleus with condensation of granular and fibrillar components of the nucleoli. The adverse immunobiological effect of OA on the developing lymphoid organs was established by histological and ultra structural studies. OA at the dose rate of 50 /ag/bird/day for a period of 60 days was administered through oesophageal intubation. Pathological changes were sequentially documented with special reference to the immune system. In the OA fed quails, the clinical signs included emaciation and hyperexcitation at the later part of the experiment. There was highly significant reduction in the body weight, weight of bursa of Fabricius, spleen and thymus. The reduction in the weight of lymphoid organs gave evidence to the adverse effects of OA on the immune system. There was reduction in the total erythrocyte count (TEC), haemoglobin (Hb) concentration and packed cell volume in OA fed birds indicating the significant damaging effect on the haemopoietic sytem. Estimation of total serum protein (TSP), serum globulin (SGI) and serum immunoglobulin fractions clarified the biological adverse effect of OA on the humoral immune system. Highly significant reduction in total leukocyte count (TLC) and T cell dependent lymphopenia proved the immunosuppressive effect of OA. There was reduction in T cells, increase migration indices in response to Fowl pox antigen in leukocyte migration inhibition test (LMIT), the reduction in the cutaneous response to 2,4-dinitrochlorobenzene (DNCB) and phytohaemagglutinin-M (PHA-M) and spleen indices in the graft versus host reaction (GVHR), clarified the immunotoxicity of OA on the cell-mediated immune system.
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    ThesisItemOpen Access
    Embryo mortality in chicken
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1987) Lalithakunjamma, C R; KAU; Krishnan Nair, M
    The nature of embryomortality in hatcheries and the posiible causes for embrypathies were studied. Experimental studies were conducted by inoculating Oxytetracycline, Ochratoxin A, Citrinin and a combination of ochratoxin A and Citrinin and avian influenza virus. Ultrastructural studies were conducted to elucidate the subcellular changes in the liver and kidneys of the embryo after administration of the mycotoxins. The various abnormalities noticed were early embryonic death, curled embryos, dead in shell, live sticky embryos, oedema of head and neck emphalitis and septicaemia, gastroachisis with herination of yolk sac, coelosoma, dwarfs, curled toe, microphthalmia, anopthalmia, brachycephaly, parrot beak, crossed beak, brachygnatha, streptosomia and a few cases of diprosopus, polymelia
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    ThesisItemOpen Access
    Prevalence and pathology of necrosis of extremities in cattle
    (Centre of Excellence in Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1989) Xavier, C J; KAU; Maryamma, K I
    A study on the prevalence and pathology of necrosis of extremities in cattle was conducted. It was found that the disease was more prevalent in buffaloes than in white cattle. The chief clinical manifestation was necrosis of tail. A close association between mouldy paddy straw and the disease syndrome was observed. Animals observed during the study were found to be free from systemic disturbances.
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    ThesisItemOpen Access
    Post-natal mortality of kids a pathoanatomical investigation
    (Department of Pathology, College of Veterinary and Animal Sciences, Mannuthy, 1980) Manomohan, C B; KAU; Krishnan, Nair M
    Gastritis was mainly manifested as a catarrhal type. Abomasal erosions, gastric ulcers and patchy necrotic abomasitis were also encountered in some cases. Enteritic lesions were seen in 72 cases of which 46 animals did not show any lesions in stomach. This included six cases of coccidiosis and one case of taeniasis. Enteritis was mainly manifested as catarrhal enteritis with infiltration of lymphocytes. Haemorrhagic enteritis and necrotic enteritis were seen in few cases. The mesenteric lymph nodes from some of these animals showed depletion of lymphocytes.