Theses

Browse

Search Results

Now showing 1 - 9 of 33

Open Access
Role of parrots in the epizootiology of newcastle disease
(Department of Microbiology, College of Veterinary and Animal Sciences,Mannuthy, 1981) Vijayan, V; Sulochana, S
The incidence, susceptibility, mode of infection and duration of excretion of the new cattle disease virus in the common Indian parrots (psittacula Krameri) were studied in detail. The blood, cloacal and throat swabs of parrots, collected from different parts of the State were screened for the presence of ND antibodies and virus. Seventeen out of 103 blood samples were found to possess HI antibodies. The serum samples which gave positive HI titres belonged to parrots kept as pets in households and allowed to mingle freely with domestic poultry and those housed in Trivandrum Zoo along with pigeons. None of the 70 cloacal and 42 throat swabs were positive for the virus. Experimental infection of parrots with undiluted virulent virus by the intranasal and intraocular routes and by the subcutaneous and intranasal routes with 1:100 dilution of the same virus gave almost the same results. All of them died within a weeks’ time, after showing symptoms of inappettance, leg and wing paralysis and diarrhoea, from day two of infection. Virus could be isolated from the throat and cloacal swabs and also from the tissue of dead birds. Chicks kept along with these infected parrots did not develop symptoms of ND, eventhough they excreted the virus, for a few days, and had a low titre of HI antibodies in their sera. All the contact chicks died of ND with typical symptoms and lesions on challenge with the virulent virus. The parrots that received a mesogenic strain (Komorov) of the virus, also succumbed to the disease, but with less pronounced symptoms and with an extended incubation period. The parrots that were infected with lentogenic strain of the virus (F1) did not develop symptoms of the disease. However multiplication of the virus occurred in these birds as isolations could be made from cloacal and throat swab and a slight increase in H1 titre was noticed sera. However on challenge with a virulent strain of NDV, they showed symptoms of ND. All of them died within eight days and the virus could be isolated from them. Contact infection of parrots from infected chicks were quite effective, as the parrots died with the same symptoms described above, almost within the same time as direct infection. Virus was also isolated from the tissues of the dead parrots. The common Indian parrots were found to be highly susceptible to both velogenic and mesogenic strains of NDV, but they were resistant to the lentogenic strain. Uninfected chicks kept along with the parrots infected with virulent virus picked up the infection, and virus could be isolated from the cloacal and throat swabs of these chicks. They also showed an increase in the antibody titre. The failure to produce clinical disease in chicken might be attributed to a decrease in virulence of the virus on passage in parrots. The carrier state with the lentogenis strain of the virus could not be assessed as they were challenged after 2 weeks. Though a carrier role had been attributed to the parakeets imported from India, the parrots in this study were found to succumb to the disease within a week. This might be due to the variation in the susceptibility of various species of parrots to NDV. The chances of dissemination of the virus could be prevented by quarantining them for a period, not less than ten days.
Open Access
Isolation and identification of viruses from waterfowls seen in Kerala
(Department of microbiology, College of veterinary and animal sciences, Mannuthy, 1997) Bindu, M S; KAU; Krishnan Nair, G
In the recent years, frequent outbreaks of poultry diseases have caused significant economic losses and among the various sources of infection, freeflying migratory birds and waterfowls are reported to be an important source for the introduction of disease to domestic flocks. These freeflying pirds and waterfowls abound in the waterlogged areas of Kerala especially Alleppey, Kottayam, Thrissur and Malappuram districts. Heavy losses were reported each year due to severe outbreaks of duck plague and duck pasturellosis resulting in the wiping out of the duck population in the state. This may be due to the introduction of the disease agents by migratory waterfowls. Hence a study was undertaken to elucidate the role of waterfowls in the spread of diseases to domestic poultry and ducks. A total of 52 waterfowls were caught from different parts of Kerala of which 15 were lesser whistling teals and 37 were gargany. Postmortem examination of these birds were carried out and the required materials were collected. Impression smears from the cut surface of the liver revealed intranuclear inclusion bodies in the hepatic cells in four cases (Bird Nos. 17, 23, 27 and 49) and two haemagglutinating agents were isolated from the cloacal swabs of the bird numbers 18 and 22. The haemagglutinating agents developed lesions in the chicken embryos which died 3 to 5 days after inoculation. the allantoamniotic fluid of the affected embryos agglutinated teal and chicken erythrocytes. The lesions exhibited by the embryos infected with Ta were congestion of the CAM and embryos while in T22 the embryos were stunted, curled and at the same time congested. The liver of the embryo had yellowish brown patches. The haemagglutination activity of both the viral isolates were tested with red cells of various species like cattle, horse, human '0', duck, rabbit and pig but was negative in all cases. Both the isolates lost their infectivity and haemagglutination property at 56°C for 30 min. the infectivity and HA activity of the viral isolates T18 and T22 were retained at pH 7.2 and were completely destroyed at pH 3.2 and pH 9. Both the isolates were sensitive to treatment with chloroform, revealing both as enveloped viruses, wherein the infectivity was completely lost and HA activity was considerably reduced. In the case of Tl8 HA activity was reduced from 512 to 16 and for T22 from 128 to 8. The nucleic acid types of the viral isolates were confirmed by inoculating the isolates to chicken embryo fibroblast cultures pretreated with 100 µg and 200 µg per ml of IudR which led to the conclusion that Ta was a RNA virus and T22 a DNA virus. The ELD50 of Ta and T22 were 107 ELD50/ml and 106 ELD50/ml. The ICPI, MDT and IVPI. were calculated as for NDV. The ICPI for the isolates were 0.83 and 0.30 for T18 and T22 respectively, MDT was 120 hr for both and the IVPI was calculated to be zero in both cases, indicating that both the isolates were nonpathogenic. In cell cultures both the isolates produced CPE which affected the whole monolayer by 96 hrs. In the case of Tl8 the CPE was characterised by rounding and clumping of cells, the infected cells showing a tendency to get separated from the neighbouring cells leaving long cytoplasmic strands, syncytium formation with four or five nuclei and severe cytoplasmic vacuolation. For T22 the CPE was characterised by rounding and cytoplasmic vacuolation and karryorhexis. Intranuclear and intracytoplasmic inclusion bodies could be demonstrated. The pathogenicity studies of both the isolates were carried out in one week old and six week old ducklings and chicken, for which both were nonpathogenic and did not exhibit any clinical signs or mortality. But viral infection was established in six week old chicks by virus isolation till the 14th day of infection from the cloacal swabs for both the isolates. The sera collected from these birds revealed an antibody titre of 16 for T¬18 and 8 for T22 indicating the infection. The antigenic relationship of the isolates was examined with NDV, EDS-76 and fish viruses (FV and F6), of which T22 did not show any antigenic similarity with any of the viruses. But T1B on the contrary exhibited antigenic relationship with the fishviruses FV and F6 but no antigenic similarity with NDV and EDS-76. The antigenic similarity exhibited by T18 with the fish viruses leads to the conclusion that waterfowls may be disseminating the viruses responsible for the outbreak of epizootic ulcerative syndrome in fishes. The morphological features of T18 by electronmicroscopy revealed an enveloped virus with a size of 150-185 nm with pleomorphic forms and peplomers of a length of 18-20 nm. Except the length of the peplomers it similated a paramyxovirus. The morphology of T22 was not studied due to technical defects.
Open Access
Antigens of pasteurella multocida isolates from rabbit and their immunologencity
(Department of microbiology, College of veterinary and animal sciences, Mannuthy, 1994) Manoharan S; KAU; Jayaprakasan, V
Two rabbit strains viz. R9 S and R23 S and a bovine vaccine strain P – 52 which were maintained in virulent form, were used for the preparation of three forms of antigen viz., heat inactivated crude extract, KSCN extract and sonicated antigen. These antigens were chemically analysed for protein and carbohydrate contents and were found to be higher in the sonicated antigen preparation irrespective of the source. In SDS – PAGE analysis, the profiles discerned by heat inactivated crude extract, KSCN extract and sonicated antigens were four, five and six protein bands with molecular weights lesser than 68 kDa while the KSCN extract and sonicated antigen presented an additional protein band with molecular weight higher than 68 kDa. Three types of antigen of P. multocida were characterized and analysed for the inter relationship and the immunogenic potential in mice. Antiserum was raised against each antigenic preparation from the three strains in rabbits and used for serological study. In AGPT and immunoelectrophoresis the serum developed multiple precipitin lines and arcs respectively when reacted against the three homologous and two heterologous antigens in which a few were identical to the heterologous antigens. The results revealed stronger serological relationship between the two rabbit strains than with the cattle strain and the heterogeneity of the sonicated antigen. The antibody titre in each antiserum was measured by IHA using the sensitized GA – SRBC/T – GA – SRBC and the titres were more in the homologous antiserum and high titre for the heterologous serum was seen with the sonicated antigen. The LD50 determined for the three strains R9 S, R23 S and P- 52 was found to be 3 x 104 , 3 x 103 and 3 x 105 bacteria. Immunogenic potential of the three antigens and an adjuvanted sonicated antigen were tested in mice by giving two doses of vaccine at 14 days interval and challenging on 21st day with homologous and heterologous strains. A higher percentage of protection was conferred by homologous strains and it was cent per cent (100%) with sonicated antigen. The percentage of protection against challenge with heterologous strains was low. An elaborated study on immunity trials with these immunogens is needed before recommending the R23 S as a candidate vaccine strain.
Open Access
Bacteria associated with respiratory infections in poultry
(Department of Veterinery Microbiology, College of Veterinary and Animal Science, Mannuthy, 2007) Jesto, George; KAU; Krishnan Nair, G
This study was undertaken to isolate and identify of bacteria from respiratory tract of poultry and to study the antibiogram of the isolates. Birds showing respiratory signs were sacrificed, postmortem examination was conducted and samples such as nasal, tracheal and air sac swabs and lungs were collected after taking all sterile precautions. A total of 105 samples were collected by sacrificing birds showing clinical signs. Isolation of causative bacteria was made by culturing on brain heart infusion agar, Mac Conkey agar and blood agar. For identification of isolates all the procedures were followed as described by Barrow and Feltham (1993). A total of 31 bacterial isolates were obtained from samples. A total of 12 Escherichia coli isolates were isolated and identified, 4 Pasteurella multocida isolates and 15 Staphylococcus sp. Isolates were isolated and identified biochemically. Out of 15 Staphylococcus sp. isolated and identified 11 isolates (73.33 per cent) were coagulase negative This result indicate that CoNS were more frequently isolated from staphylococcal infections although they do not possess the virulent coagulase activity. So importance must be given to CoNS also, as given to coagulase positive staphylococci and much study need to be diverted to find the virulence factors and role of them in producing bacterial infections in poultry. Multi drug resistance (resistance to at least three antimicrobials) was found among all E. coli isolates obtained in the study. Hence it may be concluded that the high level of resistance observed among poultry E coli isolates obtained in the study may be due to incorporation of antibiotics in feed as growth promoters. As 100 per cent sensitivity is shown to enrofloxacin and chloramphenicol by P. multocida isolates, these two drugs may be used for treating pasteurellois. Amoxycillin clavulanic acid (Ac) and cephalexin (Cp) was found to be the most effective antibiotic against Staphylococcus sp. in the study. The plasmid DNA content of the seven isolates of E. coli was analysed on agarose gel electrophoresis but correlation between the number of plasmids and antibiotic resistance could not be ascertained in this study. In conclusion, the results of this study provide evidence for significant antimicrobial resistance among bacterial isolates from birds. Long term prospective studies involving isolation, identification and antibiogram from more samples are required to identify novel pathogens causing respiratory disease in birds. Such studies provide data on temporal and spatial difference in antibiotic resistance patterns, which in turn helps the scientific community to design better disease control strategies.
Open Access
Characterization of staphylococci isolated from cases of mastitis and study of their R plasmids
(Department of microbiology, College of veterinary and animal sciences, Mannuthy, 1986) Mathew, E S; KAU; Punnoose, K T
The emergence of drug resistant staphylococci causing mastitis deserve serious investigation. The work was intended to characterize staphylococci isolated from cases of bovine mastitis and to study their R plasmid transfer in vitro. The characterization was done using glucose and mannitol fermentation, catalase production, coagulase production, Iysostaphin sensitivity and bacteriophage typing. The antibiogram of the isolates was done by agar diffusion method using 14 chemotherapeutic agents (amoxicillin, ampicillin, bacitracin, chloramphenicol, cloxacillin, erythromycin, gentamicin, methicillin, neomycin, nitrofurantoin, penicillin, streptomycin, sulphamethoxazole and tetracycline) and by agar dilution method using eight antibiotics (ampicillin, chloramphenicol, rifampicin, streptomycin, tetracycline , gentamicin, erythromycin and penicillin) The in vitro transfer of R plasmids was tried using selected S.aureus and coagulase-negative staphylococcal isolates as donors and S.aureus RN 450RF and S.epifermidis 131S as recipients. From 360 milk samples collected from cases of bovine mastitis 17 strains of S.aureus and 35 strains of coagulase negative staphylococci were isolated. Lysostaphin sensitivity test was positive for 90.38% of the isolates, but this could not be used to differentiate between coagulase positive and negative staphylococci. The results of phage typing revealed a predominance of group III phages over the other groups and the possible role of human strains of staphylococci in producing mastitis in animals. None of the strains were resistant to mercuric chloride. From the antibiogram rifampicin , bacitracin, neomycin , methicillin, gentamicin, cloxacillin, nitrofurantoin and chloramphenicol were found to be the drugs of choice in the treatment of bovine mastitis caused by staphylococci. When S. aureus Rn 450RF was used as recipient six of the ten selected S.aureus isolates could transfer either one or more drug resistance markers and the mode of transfer was suspected to be by conjugation. All the four selected streptomycin resistant coagulase –negative staphylococcal donors were found to transfer the R plasmid DNA to the recipient., S.aureus RN 450RF, which was established to be through conjugation. The drug resistant S. aureus as well as coagulase negative staphylococcal strains failed t transfer their resistance to S.epidermidis 131S.
Open Access
Characterization and immunology of inflenza virus type a isolated from duck in Kerala
(Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy, 1988) Mini, M; KAU; Sulochana, S
Ducks are generally regarded as resistant to influenza virus infection. During the early halves of 1985 and 1987 influenza A viruses were isolated from cases of respiratory disease causing 15 to 20 percent mortality in two to six week-old ducklings at Government Duck Farm, Niranam. Four strains of the virus (A/duck/India /1/85 (H9N2)-CDN, A/duck/India /2/87 (H3N?) –DT3; A/duck/India /7/87 (H3N2)-T19 and A/duck/India /14/87 (H9N?-lgl isolated from these out breaks were studied in detail with particular reference to their characteristics, Pathogenicity and immunogenicity. The strains were propagated in nine day embryonated chicken eggs by allantoic route of inoculation. All the four strains multiplied well killing the embryos in two to four days time. Though characteristic lesions were not present, the embryos were slightly congested. The chorioallantoic membranes showed moderate congestion and oedema but no pock lesions. Infectivity titers were 108.5 EID 50/0.2ml; 108.25 EID 50/0.2ml 105.75 EID 50/0.2ml and 108.5 EID 50/0.2ml respectively for CDN, DT3, T19 and lgl. The corresponding HA titres were 1:64 , 1:128, 1:64 and 1:256 The strains also multiplied well in duck embryos but no specific lesions were seen either in the embryo or on the chorioallantoic membranes. The infectivity titers were low compared to the titers obtained in chick embryos. All the strains were inactivated at 560C in 30 minutes. They lost their infectivity at PH 3.2 while HA property was not considerably reduced except for lgl. At PH 7.2 both infectivity and HA property were not affected. But infectivity was slightly affected and HA property was markedly reduced at PH 9.0. The viruses were chloroform sensitive and agglutinated red cells from cattle, sheep, goat, guinea pigs, horse, rabbit, rat mouse, chicken and man (0, A and B Groups). Chicken embryo fibroblastic infected with strain CDN produced cytopathic effects characterized by rounding of cells affecting the whole monolayer in 96 hours. The remaining three strains did not produce any CPE. In all the four cases virus infection was evidenced as the cell culture fluid gave haemagglutination. The infectivity titers of these fluids were 106.5 EID 50/0.2ml and 105.5 EID 50/0.2 ml 104.0 EID 50/0.2 ml and 105.5 EID 50/0.2 ml respectively for CDN, DT3, T19 and lgl. Two common antigens possibly the type specific MP and NP antigens were detected in the CAM extracts of embryos infected with these strains by agar gel precipitation and immunoelectrophoresis. Mean death time for these strains calculated according to the method adopted for NDV were 78 hours, 76.8 hours, 72 hours and 76 hours respectively for CDN, DT3, T19 and lgl, while the ICPI in day-old chicks were 0.325, 0.66, 0.00 and 0.16 and IVPI 0.00 in all cases. Pathogenicity and immunogenicity studies were carried out in day-old, one week-old and two week-old ducklings with strain CDN and in the week-old ducklings with strain lgl. Strain CDN produced hundred percent mortality in day old ducklings that received the virus by oral , occulonasal and subcutaneous routes. They died with no marked clinical signs except droopiness, discharges from the eyes. Slight diarhoea and ruffled feathers in some of them. The pathogenicity indices were 1.19, 1.15 and 1.51 respectively for group A,B and C. The ducklings that were kept to study contact infection revealed cloacal excretion of the virus till 12th day, through tracheal excretion stopped by 10th day. None of the birds in all the four groups that survived seven day of infection did reveal any specific seven day of infection did reveal any specific HI antibodies. In one week-old ducklings no clinical symptoms were observed. Virus could be isolated from cloacal and throat swabs before death and from tissues of dead birds. The pathogenicity indices were 0.36, 0.00 and 0.20 respectively for group A, B and C. In contact ducklings picked up infection as indicated from virus isolation and antibody titration. Both infected and incontact birds showed HI antibodies from 7th day onwards. By 14th day the titres reached peak level ranging between of 1:16 to 1:64 followed by a decline and remained steady through out the observation period. The two week-old ducklings did not show any clinical symptoms or death. But they revealed tracheal and cloacal shedding of the virus. The HI antibody titres never increased beyond 1:16. None of the incontent birds either showed clinical symptoms or death. Positive virus isolation could be made till the 7th day. Antibody response was also very poor and HI antibody titers never increased beyond 1:4. One week-old ducklings infected with strain lgl remained apparently healthy throughout the period of observation. However, virus isolations were made from cloacal and tracheal samples from 3rd to 7th day. In birds that received the virus by oral route, HI antibody titres were 1:4, 1:8 and 1:4 respectively on 7th, 14th and 21st day and nil by 28th day. In order two groups the birds did not reveal HI antibodies till 28th day. None of the incontact birds in all three groups showed clinical symptoms, death or any other indication of infection by the virus. In addition the sera from these birds were also negative for HI antibodies.
Open Access
Development and evaluation of outer membrane protein vaccine against duck pasteurellosis
(Department of Veterinary Microbiology, College of Veterinary and Animal Science, Mannuthy, 2007) Ranjini, A R; KAU; Krishnan, Nair G
A research work was undertaken to prepare effective vaccines against P. multocida grown under different conditions and their immunopotency was assessed in one month old ducklings. The purity of the P. multocida A:l strain (DP1) was confirmed as per standard procedure. Pathogenicity of the isolate was assessed in six to eight weeks old mice. The isolate killed the mice with in 8 h intra peritoneally and within 24 h when injected subcutaneously. Whole cell protein of P. multocida (DP1) was extracted and they were subjected to discontinuous system of SDS- PAGE which revealed 20 to 26 visible protein bands of molecular weight ranging from 102 to 19 kDa. After growing P .multocida in iron sufficient and iron restricted medium its OMPs were extracted and they were analysed by SDS PAGE. In iron sufficient medium, 10 protein bands of MW which ranging from 91.84 to 19.02 kDa were revealed. In addition to the above a protein band with MW of 97.8 kDa were detected in iron restricted medium.The protein concentration was estimated by Modified Lowry method,and it was found to be 4mg/ml. The Median Lethal Dose (LDso) of P. multocida when determined in one, month old ducklings was found to be 10 -7.13 which contained 13 cells. Oil adjuvanted formalin inactivated vaccines were prepared from DPl grown under three different conditions viz., TSB, BHIB and BHIB supplemented with 100 u M 2,2' dipyridyl. Sterility, safety and potency test of the vaccines were done as per standard procedures. A total of 120 one month old ducklings were divided in to four groups with 30 birds in each group. The first three groups were vaccinated with ordinary bacterin, OMP vaccine prepared under iron sufficient condition and OMP vaccine prepared under iron restricted condition respectively and the fourth group served as control.The birds were vaccinated with 0.5millilitre of vaccine intramuscularly. The blood was collected from all the ducks on day 0,7, 14,21,28,45 and 60 day PV. Passive haemagglutination was done and the increase in antibody titre was observed from day 7 PV onwards for groups I,.Il and In. The highest antibody titre was obtained at 14th day PV and 21 st day PV for aMP vaccine prepared under iron restricted condition. All the vaccine groups had shown a significant difference from the control group at all stages of study. On homologous challenging OMP vaccine under iron restricted media on 42nd day PV afforded 60 per cent protection. On 60th day PV afforded 50 per cent protection. Though the aMP vaccine prepared under iron restricted condition was found to provide high antibody titre, the protection percentage afforded by it in comparison with ordinary bacterin and aMP vaccine prepared under iron sufficient media was low. Hence, the aMP vaccine prepared under iron restricted condition need to be subjected to further investigation.
Open Access
Evaluation of pathogenicity and antigenic relationship of salmonella isolates from poultry
(Department of Veterinery microbiology, College of Veterinary and Animal Science, Mannuthy, 2007) Sunil, G; KAU; Koshy, John (Guide)
In the present study the pathogenicity and the antigenic relationship of three local isolates of S. gallinarum was compared with that of a reference/known strain. The antigenic homogeneity/heterogeneity of these isolates was studied using SDS PAGE and AGID. All the four isolates when subjected to antibiogram revealed similar pattern. The only difference was with isolate X 4 which was susceptible to co-trimoxazole while all the other three were resistant. Except for the reference/known strain BGL, all the other three isolates were found pathogenic to mice. All the four isolates were pathogenic to day-old chicks. Isolates QS 1 and X 4 were most pathogenic. Major clinical signs were somnolence, weakness, inappetance and whitish diarrhea and were more prominent in IM inoculated group. All chicks that died during the experimental period gave positive Salmonella isolation from liver, spleen, heart blood, lungs, yolk and ceca. At the end of experimental period survived birds were sacrificed and they gave positive isolation only from ceca. All the four isolates were pathogenic to layers. The clinical signs observed were listlessness, inappetance, ruffled feathers, shrunken comb, greenish-yellow diarrhea with gradual weight loss and was more prominent in IV challenged group, followed by those inoculated by oral and IC routes. The most pathogenic strain was QS 1. Cloacal swabs examined for Salmonella revealed intermittent shedding in all groups. The highest re-isolation was obtained from liver. The egg production was affected in all test groups and was most severely affected in IV group. No significant difference was noticed between different routes of inoculation and among different isolates in re-isolation from egg. The re-isolates were confirmed as S. gallinarum by standard biochemical reactions. The gross and histopathological changes observed in experimental infection with all the isolates were the same. There was enlargement and necrosis of liver and spleen, with congestion of heart and lungs. Day-old chicks had omphalitis. Histopathologically there was congestion, necrosis and infiltration of inflammatory cells into the internal organs, including submucosa of intestine. Sodium dodecyl sulphate - polyacrylamide gel electrophoresis analysis of the OMPs of all four isolates revealed 13 different polypeptide bands. These bands were of similar molecular weight in all the four isolates, indicating antigenic homogeneity. Agar gel immunodiffusion carried out using hyper immune serum raised against isolates BGL and QS 1 revealed lines of identity between the four isolates, indicating antigenic homogeneity by serology.
Open Access
Secretory immunoglobulins of the duck (Anas platyrrhynchos domesticus)
(Department of Microbiology, College of Veterinary and Animal Sciences, Mannuthy, 1996) Hari, Kumar A V; James, P C
The profile and functional properties of the immunoglobulins of serum, bile, mucos of trachea and intestine of ducks were studied. The immunoglobulins were separated by salting out using ammonium sulphate. The various fractions of immunoglobulines were further resolved by Sephadex G-200 gel filteration which gave two peaks for serum and tracheal immunoglobulins and a single peak each for bile and intestinal immunoglobulins. The purity of these fractions were checked by immunodiffusion and immunoelectrophoresis. The different fractions obtained were quantified by single radial immunodiffusion. The level of fraction 1 in the bile ranged between 1718 µg/ml and 1959 µg/ml and that of serum, 1718.06 µg/ml to 2442 µg/ml, in the S.typhimurium treated groups. The level of fraction 1 in the NDV treated groups ranged from 1115 µg/ml to 1597.35 µg/ml in bile, and 1597.35 µg/ml to 1959.34 µg/ml in serum. The level of fraction 2 in serum ranged from 1797 µg/ml to 2591 µg/ml in S.typhimurium treated group and 1400 µg/ml to 1797 µg/ml in the NDV treated group. Fraction 2 was not detectable in bile. The antibody response of ducks to a bacterial and viral antigen (anaculture of S.typhimurium and R2B strain of New Castle Disease virus respectively) was assessed. On conducting standard tube agglutination test, the serum, bile and oviduct washings revealed antibody tires against S.typhimurium in inoculated birds ranging between 1:20 and 1:160 in the case of serum; 1:10 and 1:80 in the case of bile and tire less than 1:10 for oviduct washings. No antibody titre could be detected for tracheal and intestinal washings and testicular extracts. The HI titre ranging from 1:32 to 1:128 could be observed for serum, 1:32 to 1:64 for bile and a titre of 1:16 was observed for oviduct washings of ducks parenterally administered with NDV. The tire was relatively low for serum when NDV was administered intranasaly. Intestinal and tracheal washings and testicular extract failed to reveal any HI antibodies.

Theses

Browse

Thesis Type

Type

Author

Date

Subject

Search Results