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Theses

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2001 - 20085
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Subject: Veterinary Epidemiology and Preventive Medicine × Start date: 1996 × Thesis Type: M.V.Sc. ×
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Now showing 1 - 5 of 5
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    ThesisItemOpen Access
    Clinico-therapeutic studies on bacterial mastitis in goats
    (Department of Veterinery Epidmiology and Preventive Medicine, College of Veterinary and Animal Science,Mannuthy, 2005) Sreeja, S; KAU; Vijayakumar, K
    The lactating does in the University goat and sheep farm were screened for subclinical mastitis once in three months using the California mastitis test. The occurrence of subclinical mastitis was found to be 30.2 per cent. Statistical analysis showed no significant association between occurrence of subclinical mastitis and teat length whereas there was significant association between distance from teat tip to floor. Among 642 samples screened 194 samples were found to be positive by CMT. The arithmetic mean cell counts for each CMT score ranged between 0.736 ± 0.033 x106 and 20.417 ± 0.851 x106 cells/ml. Among CMT positive samples MWST and MAMP detected 62.89 per cent and 43.29 per cent as positive for subclinical mastitis. Comparison of screening tests revealed that significant positive correlation existed among the four tests namely CMT, MWST, MAMP and SCC. Comparison with culture results showed that score ‘3’ of CMT score ‘3+’ of MWST and grade 3 of MAMP reaction detected the maximum positive cases. CMT scores and SCC in bacteriologicaly positive samples showed significant association. Among the TIST positive milk samples 20 (44.44 per cent) were culture positive. Staphylococcus aureus was the most predominant isolate in both clinical and subclinical caprine mastitis. In vitro antibiotic sensitivity pattern revealed that chloramphenicol was the most sensitive antibiotic followed by ceftriaxone and ciprofloxacin. The isolated pathogens showed maximum resistance to sulpha. Comparison of treatment trials in 24 clinical goat mastitis cases using ceftriaxone and ciprofloxacin with 12 animals in each group revealed that clinical and bacteriological cure was better in the case of ciprofloxacin. Clinical and bacteriological cure was comparatively less in gangrenous mastitis cases. Eighteen Staphylococcus isolates from clinical mastitis cases and 23 Staphylococcus isolates from subclinical cases were typed by RAPD fingerprinting. Twelve different genotypes were obtained among which genotype c predominated in clinical mastitis whereas in subclinical cases b and i were the common Staphylococcal genotypes. Clinical and bacteriological cure rates were 100 per cent for RAPD type l in the ceftriaxone treated group and genotypes c and i in the ciprofloxacin treated group of animals. A possible relationship regarding the genetic make up of the different Staphylococcal isolates was elucidated from the phylogenetic tree generated from the RAPD fingerprints.
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    ThesisItemOpen Access
    Surveillance of haemoprotozoan and haemorickettsial diseases of cattle of northern Kerala
    (Department Of Veterinary Epidemiology & Preventive Medicines,College of Veterinary And Animal Sciences, Mannuthy, 2008) Archana Nair, S; KAU; Tresamol, P V
    A cross-sectional study was conducted to assess the carrier status of haemoprotozoan and haemorickettsial diseases of cattle of Northern Kerala. The diagnostic methods employed were microscopical examination of blood smears stained with Giemsa and Acridine Orange and polymerase chain reaction. A total of 150 blood samples were collected from the five districts of Northern Kerala (30 samples from each district). Microscopical examination of Giemsa stained smears revealed Theileria like piroplasms (41.2 per cent), Babesia piroplasms (2.6 per cent) and Anaplasma inclusions (1.3 per cent) while Acridine Orange stained smears detected Theileria like piroplasms in 47.2 per cent, Babesia piroplasms in 2.6 per cent and Anaplasma inclusions in 2 per cent of blood smears examined. Acridine Orange staining is more easy, time saving and reliable in detecting of haemoparasites than Giemsa staining technique. Polymerase chain reaction detected haemoprotozoan like T. evansi (34.6 per cent), B. bigemina (0.6 per cent), an uncharacterized Theileria organism (16 per cent) and T. orientalis (1.3 per cent). A. marginale (16.7 per cent) and A. bovis (3.3 per cent) were the haemorickettsial organisms detected by PCR. Mixed infections were detected in 16.6 per cent of the samples. T.annulata, B. bovis and A. phagocytophila were not detected in any of the samples. Polymerase chain reaction showed much higher sensitivity compared to examination of stained smears in detecting carrier animals. Trypanosomosis is the most prevalent protozoan disease among carrier animals of Northern Kerala which were detected only with the help of PCR while microscopy totally failed to detect such carriers. The high prevalence of haemoprotozoan and haemorickettsial diseases suggests the abundance of ticks and flies in Northern Kerala which acts as vectors for these parasites. Kannur and Palakkad districts revealed maximum prevalence of T. evansi. In addition, anaplasmosis was also widely prevalent in Palakkad district. Microscopical examination of blood smears from cattle of the hilly districts of Wayanad, Malappuram and Kozhikode revealed the presence of a Theileria like piroplasm which were not amplified with PCRs specific for Theileria genus, T. annulata or T. orientalis and this uncharacterized piroplasm needs urgent attention.
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    ThesisItemOpen Access
    Comparative efficacy of diagnostic tests for paratuberculosis in goats
    (Department of Veterinery Edodomology and Preventive Medicine, College of Veterinary and Animal Science, Mannuthy, 2008) Sulficar, S; KAU; Saseendranath, M R
    Paratuberculosis (Johne’s disease) is a chronic debilitating infection of goats caused by Mycobacterium avium subsp paratuberculosis (MAP). Recently the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the present study comparative efficacy of acid fast staining of faecal smear, single intradermal Johnin test and IS900 faecal PCR were investigated in two hundred goats for detection of MAP. Single intradermal Johnin test was carried out in two hundred goats. Faecal sample from all two hundred goats subjected to Ziehl-Neelsen acid fast staining and IS900 PCR for detection of MAP. Out of one hundred and fifty goats screened for paratuberculosis from University sheep and goat farm, Mannuthy, five animals (3.33 per cent) were found positive by acid fast staining, eight animals (5.3 per cent) were found positive by single intradermal Johnin test and thirty animals (20 per cent) were found positive by IS900 PCR. Out of fifty goats screened for paratuberculosis from field, one goat (2 per cent), three goats (6 per cent) and twelve animals (24 per cent) were found positive by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS900 PCR respectively Results of present study from both farm and field indicate that amplification of the IS900 insertion element was the most specific and sensitive diagnostic method. The strategic use of PCR can provide a means for early identification of MAP infected goat, thus ensuring their elimination from an infected herd. Advantage of skin test is its ease of application and its low cost. Culling of CMI test positives could be a cost effective means of removing infected animals before they actually start faecal shedding. Ziehl-Neelsen staining had the lowest detection rate of the three tests under investigation and it proved unreliable in diagnosing Johne’s disease.
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    ThesisItemOpen Access
    Seroprevalence of peste des petits ruminants in goats of Kerala
    (Department of Veterinary Epidomology and Preventive Medicine, College of Veterinary and Animal Science, Mannuthy, 2007) Janus, A; KAU; Tresamol, P V
    Seroprevalence of PPR in goats of Kerala was studied using competitive ELISA. Four hundred and twelve sera samples were collected from goats belonging to different places of Kerala covering all the districts. Samples were collected randomnly from goats of different age , managemental practice, breed, sex, different health status and also from different farms. Samples were subjected to c ELISA for detecting antibodies against PPR infection. Out of 412 sera samples tested 15.5 per cent gave positive result for PPR antibodies. Seroprevalence of PPR antibodies was more in animals reared under organized farming system. Seroprevalence of PPR antibodies was more in animals with a history of disease than apparently healthy animals. Prevalence of PPR antibodies is found to be more in malabari breeds of goats when compared to jamunapari and cross bred animals. Seroprevalence of PPR in various age group revealed that percentage of positivity was more in animals of the age group of 6 months- one year. Animals from different farms were tested for antibodies against PPR infection. Seroprevalence of PPR was more in KLDB goat farm, Dhoni, and Jersey farm, Vithura. Seroprevalence of PPR among goats with different clinical manifestations was recorded. High percentage of seroprevalence was noted in animals with abortion. Seroprevalence of PPR in different districts of Kerala was assessed. The highest seroprevalence was recorded in Kozhikkode district. Seroprevalence of PPR in goats of Kerala was detected as 15.5 per cent
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    ThesisItemOpen Access
    Clinico-therapeutic studies on bacterial mastitis in bovines
    (Department of Veterinary Epideminology and Preventive Medicine, College of Veterinary and Animal Sciences,Mannuthy, 2001) Smitha Rose Sebastian, KAU; Vijayakumar, K
    A study was carried out to detect subclinical mastitis by California mastitis test, to compare the different mastitis indicators, to identify the bacterial agents responsible and their antibiogram and to assess the efficacy of different treatments adopted. The occurrence of subclinical mastitis in organized farms and in and around Thrissur was found to be 25.17 per cent, after examining 1053 quarter milk samples by California mastitis test. A total of 265 milk samples were subjected to somatic cell count, modified Whiteside test and modified Aulendorfer mastitis probe test. These tests showed a positive correlation with somatic cell count. Staphylococcus aureus was found to be the chief etiological agent followed by coagulase negative staphylococci, coliforms and Streptococcus agalactiae. Chloramphenicol and enrofloxacin were found to be the most effective antibiotics against bacterial isolates from mastitis cases and sulphadiazine and trimethoprim the least effective. In vivo studies revealed that oxytetracycine is more effective followed by enrofloxacin and amoxycillin-cloxacillin combination. But the bacteriological cure was same for all the treatments. The twenty four Staphylococcus aureus isolated from clinical mastitis were grouped into seven different genotypes using RAPD analysis. The four Escherichia coli isolates gave single RAPD pattern. S. aureus genotype a was found to be more prevalent.