Thumbnail Image

Theses

Browse

20082
Reset filters
Subject: Veterinary Epidemiology and Preventive Medicine × End date: 2008 × Start date: 2008 ×
Reset filters

Search Results

Now showing 1 - 2 of 2
  • Thumbnail Image
    ThesisItemOpen Access
    Surveillance of haemoprotozoan and haemorickettsial diseases of cattle of northern Kerala
    (Department Of Veterinary Epidemiology & Preventive Medicines,College of Veterinary And Animal Sciences, Mannuthy, 2008) Archana Nair, S; KAU; Tresamol, P V
    A cross-sectional study was conducted to assess the carrier status of haemoprotozoan and haemorickettsial diseases of cattle of Northern Kerala. The diagnostic methods employed were microscopical examination of blood smears stained with Giemsa and Acridine Orange and polymerase chain reaction. A total of 150 blood samples were collected from the five districts of Northern Kerala (30 samples from each district). Microscopical examination of Giemsa stained smears revealed Theileria like piroplasms (41.2 per cent), Babesia piroplasms (2.6 per cent) and Anaplasma inclusions (1.3 per cent) while Acridine Orange stained smears detected Theileria like piroplasms in 47.2 per cent, Babesia piroplasms in 2.6 per cent and Anaplasma inclusions in 2 per cent of blood smears examined. Acridine Orange staining is more easy, time saving and reliable in detecting of haemoparasites than Giemsa staining technique. Polymerase chain reaction detected haemoprotozoan like T. evansi (34.6 per cent), B. bigemina (0.6 per cent), an uncharacterized Theileria organism (16 per cent) and T. orientalis (1.3 per cent). A. marginale (16.7 per cent) and A. bovis (3.3 per cent) were the haemorickettsial organisms detected by PCR. Mixed infections were detected in 16.6 per cent of the samples. T.annulata, B. bovis and A. phagocytophila were not detected in any of the samples. Polymerase chain reaction showed much higher sensitivity compared to examination of stained smears in detecting carrier animals. Trypanosomosis is the most prevalent protozoan disease among carrier animals of Northern Kerala which were detected only with the help of PCR while microscopy totally failed to detect such carriers. The high prevalence of haemoprotozoan and haemorickettsial diseases suggests the abundance of ticks and flies in Northern Kerala which acts as vectors for these parasites. Kannur and Palakkad districts revealed maximum prevalence of T. evansi. In addition, anaplasmosis was also widely prevalent in Palakkad district. Microscopical examination of blood smears from cattle of the hilly districts of Wayanad, Malappuram and Kozhikode revealed the presence of a Theileria like piroplasm which were not amplified with PCRs specific for Theileria genus, T. annulata or T. orientalis and this uncharacterized piroplasm needs urgent attention.
  • Thumbnail Image
    ThesisItemOpen Access
    Comparative efficacy of diagnostic tests for paratuberculosis in goats
    (Department of Veterinery Edodomology and Preventive Medicine, College of Veterinary and Animal Science, Mannuthy, 2008) Sulficar, S; KAU; Saseendranath, M R
    Paratuberculosis (Johne’s disease) is a chronic debilitating infection of goats caused by Mycobacterium avium subsp paratuberculosis (MAP). Recently the organism was reported to be associated with enteric infection in humans and hence the disease is of public health importance. In the present study comparative efficacy of acid fast staining of faecal smear, single intradermal Johnin test and IS900 faecal PCR were investigated in two hundred goats for detection of MAP. Single intradermal Johnin test was carried out in two hundred goats. Faecal sample from all two hundred goats subjected to Ziehl-Neelsen acid fast staining and IS900 PCR for detection of MAP. Out of one hundred and fifty goats screened for paratuberculosis from University sheep and goat farm, Mannuthy, five animals (3.33 per cent) were found positive by acid fast staining, eight animals (5.3 per cent) were found positive by single intradermal Johnin test and thirty animals (20 per cent) were found positive by IS900 PCR. Out of fifty goats screened for paratuberculosis from field, one goat (2 per cent), three goats (6 per cent) and twelve animals (24 per cent) were found positive by Ziehl-Neelsen acid fast staining of faecal smear, single intradermal Johnin test and IS900 PCR respectively Results of present study from both farm and field indicate that amplification of the IS900 insertion element was the most specific and sensitive diagnostic method. The strategic use of PCR can provide a means for early identification of MAP infected goat, thus ensuring their elimination from an infected herd. Advantage of skin test is its ease of application and its low cost. Culling of CMI test positives could be a cost effective means of removing infected animals before they actually start faecal shedding. Ziehl-Neelsen staining had the lowest detection rate of the three tests under investigation and it proved unreliable in diagnosing Johne’s disease.