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  • ThesisItemOpen Access
    Studies on the postnatal development of the epididymis in the Malabari goat (capra hircus)
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1975) Harshan, K R; KAU; Radhakrishnan, K
    The postnatal development of epididymis was described and illustrated from studies in 39 Malabari goats, divided into 13 groups, ranging from day old to 180 days of age. The weight of epididymis was positively correlated to age and body weight. The epididymal weight was more significantly correlated to body weight than to age. The left epididymis was found to be significantly heavier than the right. The length and thickness of the head, the body and the tail of the epididymis increased with increase in the weight of the organ. The tunica albuginea was found to be fibro- muscular in structure. The interstitial tissue was fibrous. The diameter of the epididymal duct and its lumen increased with increase in age. Both these measurements were highest in the cauda, less in the corpus and least in the caput. The process of pseudostratification of the epithelial lining of lumen of the duct was completed at first in the cauda, secondly in the corpus and finally in the caput. Regional differences in the height of epithelium was noticed, the highest being in the caput, lower in the corpus and lowest in the cauda. The epithelial height increased with advance in age. Sperms were observed at 165 days of age in the lumen of the epididymal duct. The concentration of sperm was maximum in the cauda, slightly less in the corpus and least in the caput. The lumen of the epididymis of the 150 days and 165 days old goats showed some spherical cells with spherical nuclei. These were considered to be spermatogenic cells coming from the testis.
  • ThesisItemOpen Access
    Anatomy and histology of ruminant stomach of goat
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1981) Jose John, Chungath; KAU; Radhakrishnan, K
    Gross and microscopic anatomy of the stomach of twenty adult goats, including blood supply and nerve supply have been investigated. Linear, weight and capacity measurements were recorded in relation to body length and weight. The disposition of inner mucosa of various stomach compartments was also studied. The caudo-ventral blind sac of rumen of goat, unlike that of other ruminants, extended more caudally than the dorsal one. Dorsal coronary grooves were absent. The right ventral coronary groove was distinct and reached the ventral border of rumen. The omasum was the smallest and oval in shape. The abomasum was comparatively larger in goats. The compound stomach in adult goat weighed 3.45 per cent of intact body weight. The volume of different stomach compartments as percentage of total stomach were 73, 7, 2 and 18 for rumen, reticulxam, omasiim and abomasum respectively. The wet tissue weights of different compartments as percentage of total stomach were about 66, 9, 8 and 17 for rumen, reticuliim, omasum and abomasum respectively. Basically, the mlcroscopic structure of goat stomach resembled closely that of other uruminants. The forestomach had the non-glandular cutaneous mucous membrane, a connective tissue sub mucosa, a two layered muscular tunic and a serosa. The omasum and abomasum presented the muscularis mucosae. The abomasum had glandular epithelium with the cardiac, fundic and pyloric regions. The different compartments of stomach of goats was innervated by the dorsal and ventral branches of the vagus. The arterial supply was by the branches of coelic artery.
  • ThesisItemOpen Access
    Postnatal Development of the Oviduct in the Japanese Quail (Coturnix coturnix japonica)
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1994) Lucy, K M; KAU; Harshan, K R
    The structure and postnatal development of the oviduct in quails were investigated using 72 birds aged from day-old to 60 days. The growth, morphology and histology of the oviduct were studied using six birds at each age group. In the day-old quail chick, the oviduct could seen as a narrow white translucent tube towards the left side of the coelom connected by dorsal and ventral ligaments. The sign of coiling was evident from 25 days of age. In the initial stages, the increase in weight and length of oviduct was in accordance with the growth of the bird. Rapid changes in the development of the organ occurred between 30 and 4 0 days of age and a spurt in growth was noticed from 40-60 days of age. In the day-old chick, the cranial regions of the oviduct corresponding to the infundibulum, magnum and isthmus were undifferentiated. Throughout the length of the oviduct, histological appearance was the same. The mucosa was thrown into low primary folds lined by simple columnar epithelium and there was subepithelial connective tissue containing densely packed cells with fine collagen and reticular fibres. The large number of luminal epithelial cells and the increase in height of the mucosal folds indicated entry into a rapid growth phase which started between 30 and 40 days of age. In the adult bird, the innermost epithelium of the different segments of the oviduct consisted of ciliated columnar cells and secretory goblet cells. Lamina propria was packed with tubular glands except in the funnel of infundibulum, magnum-isthmus junction and vagina. Bundles of collagen and a few elastic and reticular fibres constituted the core of the mucosal folds. Mucosal folds also contained lymphocytes in diffuse form and nodular aggregates. Thickness of the tunica muscularis increased from the anterior to the posterior end of the oviduct. Externally there was a typical serosa
  • ThesisItemOpen Access
    Post-hatch development of preen gland in the duck (anas platyrhynchos)
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 2005) Rajathi, S; KAU; Ashok, N
    The study on the post-hatch development of the preen gland in ducks was conducted using 44 ducks from the day of hatch to 150 days of age. The project was taken up to trace the structure and development of the glands and their relationship with the age and body weight. After recording gross relations and measurements, the material was fixed using various fixatives for studying the cellular details, arrangement of cells, connective tissue framework, micrometry and histochemistry. The preen gland was a paired organ with two gland and a common cylindrical papilla together formed a ‘V’ shaped structure. Each gland was pear shaped and pale yellow in colour, in fresh state. They were located on the dorsal surface of the pygostyle. The two glands had separate ducts. The uropygial circlet was seen at the tip of the papilla. The glands were vascularized through a pair of branches from the caudal artery and innnervated through the medial caudal nerve. The weight of the preen glands increased progressively from the day of hatch to 150 days of age. This weight was positively correlated with the age and body weight. The proportion of the gland weight to body weight showed a decreasing trend. The right gland was slightly heavier, longer, wider and thicker than the left. The length, breadth and thickness were positively correlated with age and body weight. Simple, branched, tubular and holocrine type of glands was covered by highly vascular connective tissue capsule composed of collagen and reticular fibres. Elastic and smooth muscle cells were absent. The secretory tubules showed two zones, an outer zone or zone I, near the capsule and an inner zone or zone II, towards the primary cavity. The epithelium of the tubules consisted of basal, intermediate and transitional cell layers. The papilla had two ducts, which were lined by glandular epithelium initially and keratinized type of stratified stratified squamous epithelium at the tip. The glandular epithelium was surrounded by longitudinally arranged smooth muscle fibres and skin. Lamellar corpuscles and circlet feather follicles were noticed in the papilla. Capsule, trabeculae and the parenchyma were PAS positive. Acid mucopolysaccharides and glycogen were not detected in the gland. Lipids were evident uniformly in all the cell layers. The acid phosphatase activity was moderate in the basal and intermediate layers and strong in the transitional layer. The alkaline phosphatase activity was moderate in the basal and intermediate layers and weak in transitional layer of outer zone. It was moderate in the basal and intermediate layers and intense in the transitional layer of inner zone. Micrometrical findings on the capsule thickness, width of outer and inner zones and the primary cavity increased with the advancement of age.
  • ThesisItemOpen Access
    Studies on the postnatal development of testis in Malabari goat
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1975) Unnikrishnan, M D; KAU; Radhakrishnan, K
    A detailed study on the postnatal development of testis is undertaken using 39 pairs of testis of goats belonging to Malabari breed. The animals varied in age from one day to 180 days. The experimental animals were divided into 13 groups having an age difference of 15 days between groups. It was observed that the testicular weight was positively correlated with age and body weight of the animal. The growth of testis could be divided into two phases. A slow gradual increase in weight until about 135 days of age and a sharp rise thereafter till adulthood. The left testis was found to be more heavier than the right testis in the same animal. During growth, the shape of the organ was not altered. The diameter or seminiferous tubule was found to be positively correlated with age, body weight and testicular weight of the animal. Weight of testis was found to be having a greater influence on the diameter of seminiferous tubule than age or body weight. Goat testis follows five phases of postnatal development based on histological changes. It was established that a Malabari buck attains sexual maturity by the age of 180 days.
  • ThesisItemOpen Access
    Postnatal development of the bursa of fabricius in duck (Anas platyrhynchos)
    (Department of Anatomy, College of veterinary and animal sciences, Mannuthy, 1999) Indu, V Raj; KAU; Jose John Chungath
    The structure and post natal development of the bursa of Fabricious in White Pekin ducks were investigated using 51 birds aged from day – old to 155 – days. The growth, morphology and histology of the bursa were studied using three birds of each age group. In the day – old ducklings, the bursa could be seen as a smooth, yellow, elongated blind sac – like structure with a tapering apex. By 155 days it was a cylintrical and much reduced pale structure. In all the birds, the bursa communicated with the proctodeum of cloaca by a short stalk. The inner surface of bursa contained two large well – developed plicae on the ventral aspect and about five to eight smaller folds all round the circumference. The number of plicae increased upto 30 days of post natal life. After 80 days a decreasing trend was recorded in their number. After hatch, the bursal growth rate was not in proportion to that of body. It showed a decreasing trend after attaining peak values at five days of age. Though the bursa weight varied with variation in the age and body weight of the bird, a greater percentage variation in its weight was accounted for by body weight. The weight, length, diameter and plical measurements of bursa attained maximum average values at 58 days of age indicating that the bursa of ducks may be most functional at this age. Histologically, the wall of the bursa was divided into three tunics in birds of all ages. The outermost, tunica serosa enveloped the entire organ and increased in thickness gradually. The middle, tunica muscularis consisted of an outer circular and inner longitudinal layer of smooth muscle fibres with blood vessels in between. The innermost, tunica mucosa consisted of pseudostratified lining epithelium and lamina propria filled with follicles. The epithelium was distinguished into follicle associated epithelium and interfollicular epithelium. Each follicle consisted of a cortex and medulla separated by a layer of epithelial cells with distinct basement membrane in birds of all ages. Lymphoblast, lymphocytes and macrophages formed the cellular component of the follicle. The number, size and cellular details of lymphoid follicles attained their peak – values by about 58 to 65 days of post hatch period. The interfollicular and subepithelial connective tissue was made up of collagen and reticular fibres with a few elastic fibres. The cellular component in it included plasma cells, eosinophils, mast cells, macrophages and fat cells. Involutory changes in the bursa were recorded from 95 – days post hatch characterised by degeneration of plical epithelium and follicular atrophy. Prominent microscopic features of involution were evident from 140 days of age. The bursa showed follicular degeneration, fibrosis of subepithelialstroma, collapse of plicae, depletion of lymphocytes and fatty replacement of the organ. The lining epithelial cells of bursal mucosa revealed positive reaction to Schiff’s reagent and metachromasia in birds of all ages. Intense acid phosphatase and moderate alkaline phosphatase activity was noticed in the epithelial cells of the bursa, in all the birds. The maximum positive immunoperoxidase activity seen by about 35 to 58 days of age suggested that immunologically the bursa was at its peak functional activity at this age.
  • ThesisItemOpen Access
    Postnatal development of the upper digestive tract in the Japanese quail
    (Department of Anatomy, College of veterinary and animal sciences, Mannuthy, 1995) Maya, S; KAU; Lucy Paily
    The structure and postnatal development of the upper digestive tract of Japanese quails were studied using 72 birds aged from day-old to 60 days for proper understanding of their functional peculiarities. The growth, morphology and histology of the tongue, pharynx, cervical and thoracic parts of oesophagus, crop, proventriculus and gizzard were studied using six birds at each age group. The age groups studied were at three days interval upto 15 days and thereafter at five days interval upto 30 days and then at ten days interval upto 60 days of age. Maximum weight of each part was recorded at 60 days of age. But maximum contribution to body weight by each part occurred within the first week of age. The tongue, pharynx and oesophagus were lined by stratified squamous epithelium, which showed keratinization on the anterior two-third of the ventral surface of the tongue and on the papillae of the tongue and pharynx. Filiform, conical and fungiform papillae were present on the base of the tongue. The rostral lingual glands contained both serous and mucous end pieces, but posterior glands showed only mucous cells. The taste buds were associated with the glandular ducts. The skeleton of the tongue showed signs of ossification from 30th day of age, with the tip of the entoglossal bone remaining cartilaginous even upto 60 days of age. In the pharynx, pharyngeal tonsil and palatine, sphenopterygoid and cricoarytenoid salivary glands were noticed. In addition to mucous and pieces the medial palatine glands exhibited serous and pieces also. Within the choanal slit and laryngeal inlet the squamous epithelium changed into respiratory epithelium. Laryngeal cartilages were cricoids, procricoid and paired arytenoids. The oesophagus was represented by a long cervical and short thoracic parts with a diverticulum, the crop. The epithelium on the top of the longitudinal mucosal folds was higher in the cervical oesophagus. Mucous glands were present throughout the oesophagus and crop but much reduced in the greater curvature of the crop and lymphoid tissue was absent at the region. Muscularis mucosa and submucosa entered into the longitudinal folds. Tunica muscularis consisted of iner circular and outer longitudinal layers of smooth muscles. Tunica adventitia was the outermost layer. A well developed oesophageal tonsil was present at the oesophago- proventricular junction. THh spindle-shaped proventriculars had a mucosa studied with minute nodules. Surface epithelium consisted of cuboidal to columnar cells. Lamina propria contained simple tubular glands. Muscularis mucosa consisted of scattered bundles of longitudinagl smooth muscle fibres. The compound tubular glands in the submucosa were lined by oxyntico peptic cells which had a dentate appearance. Tunica muscularis consisted of inner and oter longitudinal and middle circular layers of smooth muscle fibres. Outermost was the serosa. At the isthmus, the glands and outer longitudinal muscle layer of proventriculus disappeared and the lamina propria contained glands similar to those of gizzard. The biconvex dis-shaped gizzard had a greater dorsoventral diameter than cranio – caudal diameter. Internal to the serous lining was the tendon layer which was thickest at the tendinous aponeurosis and absent at the blind sacs. The lateral muscles consisted of a single layer of smooth muscle where as the blind sacs had inner longitudinal and outer circular muscle layers. Submucosa was dense and the muscularis mucosa absent. The tubular gland in the lamina propria were lined mainly by chief cells, and few basal and intermediate cells.Tall columnar cells which showed supranuclear PAS positive reaction, constituted the surface epithelium. The gizzard lining consisted of arrays of vertical columns, secreted by the tubular glands and a matrix produced by surface cells. The junction of gizzard with duodenum was marked by a constriction of muscularis mucosa forming a fold of the muscularis and tunica propria. Posterior to the fold spatula –shaped duodenal villi and coiled tubular glands with goblet cells were observed.
  • ThesisItemOpen Access
    Postnatal development of upper digestive tract in the duck
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1990) Shyla Paulose; KAU; Ommer, P A
    The structure and postnatal development of the upper digestive tract of ducks at different stages of growth was studied using 72 white pekin ducklings aged from day-old to 180 days for the proper understanding of their functional peculiarities. The growth, morphology and histology of the tongue, pharynx, oesophagus and crop, proventriculus and gizzard were studied using six birds at each age group. The oesophagus and the gizzard attained the maximum growth by 75 days whereas the tongue, pharynx, and the proventriculus at 150 days of age. The tongue, pharynx and oesophagus were lined by stratified squamous epithelium. Herbst’s, Grandry’s and Meissaner’s corpuscles, ganglion cells and encapsulated spherical bodies were present in the tongue. Filiform, fungiform and conical papillae, many taste buds, anterior and posterior lingual holocrine mucous glands were observed in the tongue. The tip of the entoglossal bone remained cartilaginous and showed signs of ossification from eighth day of age. Within the choanal slit, cavernous tissue covered by pseudostratified ciliated columnar epithelium was present. In pharynx, pharyngeal tonsils, palatine, sphenopterygoid and laryngeal salivary glands were noticed. At the inlet of the larynx, the epithelium changed to pseudostratified ciliated columnar. The laryngeal cartilages were cricoids, procricoid and paired arytenoids. The oesophagus consisted of longer cervical part, spindle shaped crop and short thoracic part. The longitudinal mucosal folds were more at the crop and the microscopic papillae appeared on the crop and thoracic oesophagus from 15 days of age. The mucous glands were present throughout the esophagus and crop. The muscularis mucosa and submucosa were absent. Tunica muscularis consisted of inner longitudinal and outer circular layer of smooth muscles. The adventitial cost was replaced by a layer of serosa in the thoracic part. A well developed oesophageal tonsil was present at the oesophage –proventricular junction. The elongated proventriculus had narrow cranial and wider caudal ends with numerous small papillae containing the opening of the proventricular gland. The mucosal folds were lined by cuboidal to columnar cells. The superficial proprial glands showed zonation. The unilobular compound tubular glands were lined by oxyntico-peptic cells which had a dentate appearance. In the day –old ducklings, the lobules were elongated in the anterior and middle part and in the posterior portion they were small and polygonal in shape. From 15 days onwards, most of the gland lobules were round or polygonal in cross section. The central cavity of the gland and the duct were lined by tall columnar cells which were PAS positive. The muscularis mucosa as a distinct layer was not seen and the submucosa was absent, Tunica muscularis consisted of inner and outer longitudinal and middle circular layer of smooth muscles and invested by the serous layer. At the isthmus, the proventricular glands and the outer longitudinal muscle layer were absent and the lamina propria contained glands similar to gizzard glands. The gizzard had greater dorso-ventral diameter than cranic –caudal diameter except in day-old ducklings in which the reverse was true. The tendon layer was thickest at the tendinous aponeurosis and absent in the blind sacs. The lateral muscle consisted of single layer of circular smooth muscle whereas the blind sacs had inner longitudinal and outer circular layers of smooth muscle. Submucosa was dense and the muscularis mucosa was absent. The simple tubular gizzard gland was lined mainly by chief cells. The chief cell granules and the luminal contents showed similar reactions in PTAH and trichrome staining. The surface epithelium showed papillary projections and were lined by tall columnar cells which showed supranuclear PAS positive reaction. The thickness of the glandular layer and the gizzard lining consisted of arrays of vertical columns secreted by the tubular glands and the matrix produced by the surface cells. The lining was strongly adherent to the musculature and could not be peeled off. The blood and nerve supplies to these organs were studied using embalmed carcasses and angiograms.
  • ThesisItemOpen Access
    Postnatal development of the oviduct in the Japanese quail (Coturnix coturnix japonica)
    (Department of Anatomy, College of Veterinary and Animal Sciences, Mannuthy, 1994) Lucy, K M; KAU; Harshan, K R
    The structure and postnatal development of the oviduct in quails were investigated using 72 birds aged from day-old to 60 days. The growth, morphology and histology of the oviduct were studied using six birds at each age group. In the day-old quail chick, the oviduct could seen as a narrow white translucent tube towards the left side of the coelom connected by dorsal and ventral ligaments. The sign of coiling was evident from 25 days of age. In the initial stages, the increase in weight and length of oviduct was in accordance with the growth of the bird. Rapid changes in the development of the organ occurred between 30 and 40 days of age and a sprut in growth was noticed from 40-60 days of age. In the day-old chick, the cranial regions of the oviduct corresponding to the infundibulum, magnum and isthmus were undifferentiated. Throughout the length of the oviduct, histological appearance was the same. The mucosa was thrown into low primary folds lined by simple columnar epithelium and there was subepithelial connective tissue containing densely packed cells with fine collagen and reticular fibres. The large number of luminal epithelial cells and the increase in height of the mucosal folds indicated entry into a rapid growth phase which started between 30 and 40 days of age. In the adult bird, the innermost epithelium of the different segments of the oviduct consisted of ciliated columnar cells and secretary goblet cells. Lamina propria was packed with tabular glands except in the funnel of infundibulum, magnum-isthumas junction and vagina. Bundles of collagen and a few elastic and reticular fibres constituted the core of the mucosal folds. Mucosal folds also contained lymphocytes in diffuse form and nodular aggregates. Thickness of the tunica muscularis increased from the anterior to the posterior end of the oviduct. Externally there was a typical serosa. Mucosal ridges of the thin walled funnel of the infundibulum were narrow and at the bases of these ridges were the glandular grooves. Tunica muscularis consisted of circularly arranged fibres and scattered bundles. Within the neck of the infundibulum, height of the mucosal ridges was more. Lining cells of the tubular glands showed PAS +ve reaction. Magnum was the longest and most coiled component of the oviduct, the wall of which was thicker due to the greatest development of tubular glands. Mucosal ridges of the isthumus were angular in appearance. Apex of the lining epithelium presented glycogen granules. Tunica muscularis consisted of inner circular and outer poorly developed longitudinal smooth muscle separated by loose connective tissue and blood vessels. Uterus was wider and thinner than the cranial portions in day-old bird itself. Secondary mucosal folds and scattered muscle fibres could be noticed at 15 days of age. In the adult bird, mucosal was thrown into numerous long, flat, discontinuous, spatula – shaped folds lined by alternating spical and basal cells. Lamina propria was loosely packed with tubular glands. Tunica muscularis was better developed with inner circular and irregular bundles and outer longitudinal muscle layer. Ultero – vaginal junction was characterized by the presence of sperm – host glands. In the vagina, mucosal folds were narrow and regular. Lining epithelium was higher . Musculature was thickest in this region. Acid and alkaline phosphatase activities were detected throughout the oviduct, the greatest activity being in the uterine region.