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  • ThesisItemOpen Access
    Seroprevalence and Diagnosis of Canine ParvoViral infection
    (Department of Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy, 1999) Deepa, P M; KAU; Saseendranath, M R
    The prevalence of canine parvoviral infection in three hundred and forty nine dogs from all over Kerala including 57 clinically suspected dogs and 292 healthy and vaccinated dogs was studied. Among 57 clinically suspected dogs, 15.7 per cent and 19.3 per cent respectively, had CPV antigen in the faecal samples by AGID and CIEP. Canine parvovirus (CPV) antigen were detected in faecal samples of 61.4 per cent of 57 clinically suspected dogs by HA test. EM detected CPV in one (13 per cent) of eight clinically suspected dogs and 17 per cent of six clinically suspected dogs had CPV in the faecal samples by IEM. Agar gel immunodiffusion test detected canine parvoviral antibody in seven (12.3 per cent) of clinically suspected dogs, 14 (36.8 per cent) of 38 healthy vaccinated dogs and 17 (6.7 per cent) of 254 healthy non-vaccinated dogs. Counterimmunoelectrophoresis (CrE) test detected CPV antibody in 8 (14 per cent) out of 57 clinically suspected dogs 14 (36.8 per cent) of 38 healthy vaccinated dogs and 17 (6.7 per cent) of 254 healthy non-vaccinated dogs. Out of 349 dogs screened by HI test, 70.5 per cent dogs had the protective level of antibody against canine parvoviral infection. Haemagglutination inhibition test detected CPV antibodies in 36 (63.2 per cent) of 57 clinically suspected dogs, 37 (97.3 per cent) of 38 healthy vaccinated dogs and 173 (68.1 per cent) of 254 healthy non-vaccinated dogs. Dot-ELISA detected CPV antibodies in 38 (66.7 per cent) of 57 clinically suspected dogs, 37 (97.3 per .cerit ) of 38 healthy vaccinated dogs and 174 (68.5 per cent) of 254 healthy non-vaccinated dogs. More seropositive dogs were recorded in dogs below six months of age. Prevalence rate observed was more in male dogs, eventhough no significant difference was observed between sexes. Among the breeds, German shepherd were more affected, followed by Doberman Pinscher and non-descript. Seasonal prevalence in the present study was higher during April, June and July. However, no significant difference was observed between different months.