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Theses

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1984 - 198923
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Subject: Plant Pathology × End date: 1989 × Start date: 1984 × Type: Thesis × Thesis Type: M.Sc ×
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    ThesisItemOpen Access
    Varietal screening of banana against anthracnose disease
    (Department of Plant Pathology, College of Horticulture, Vellanikkara, 1984) Srinagesh, K L; KAU; Jose, P C
    Laboratory and field studies of the varietal screening of banana against anthracnose disease were conducted at the college of Horticulture. Vellanikkara and at Banana Research Station, Kannara respectively during 1981-1983. In the field the infection started at the distal end of the banana fruit and in course of time the infected fruit became blackened, shriveled and mummified. After Harvest, the symptoms appeared as small brown spots which enlarged quickly and coalesced forming larger patches. The affected areas were covered with orange to salmon pink coloured conidial masses. The detailed morphological studies of the fungus proved that the anthraemose disease of banana is caused by colletrichum cloeosporioides cooko and massee, the imperfect stage of glomerella cinoulata spauld and shrenk. Twenty five varieties of banana fruit were screened in vitro at different stages of development against anthracnose disease. The varieties showed different degrees of susceptibility at various developmental stages of the fruit. The pooled analysis of the data showed that the variety nendra padaththi followed by palayankodan, jurmani kunthali, boodida bontha bathes, peyan, kanchikela, pisang mas and kapok were found to be highly resistant. The varieties Zanzibar, adakka kunnan, klue teparod, chinia, nendran, venneettu mannan, koduppilla kunnan, hybrid sawai, poocha kunnan, red banana and boodles altafort were found to be resistant to the disease. The variety robusta was found to be susceptible. The varieties njalipoovan, pisang lilin, dwarf Cavendish, matti and gros Michel were found to be highly susceptible. The major chemical constituents of banana fruit viz. reducing sugars, total sugars starch, crude fibre, crude protein and tannin at different developmental stages of twenty five varieties were analysed. The reducing sugars and total sugar were found to increase steadily from immediately after female phase to ripened stage in all the varieties. The starch and crude fibre contents, though increased steadily upto full maturity. Declined sharply at the ripening stage. The crude protein and tannin contents were maximum at immediately after female phase but steadily decreased and were minimum at ripening phase. There was a significant positive correlation between reducing sugars, total sugars and per cent disease intensity at three fourth maturity. High sugars were responsible for susceptibility to the disease. A significant negative correlation was obtained between crude protein and per cent disease intensity at half maturity. A significant negative correlation was also obtained between tannin and per cent disease intensity at one fourth and half maturity stages. High crude protein and high tannin contents were responsible for resistance to the disease.
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    ThesisItemOpen Access
    Antibiotic producing and antagonistic microorganisms in the forest soils of Kerala
    (Department of Plant Pathology, College of Horticulture, Vellanikkara, 1988) Vinod, P B; KAU; Sukumara Varma, A
    The antibiotic producing and antagonistic fungi, actinomycetes and bacteria in the evergreen forest soils of Ladysmith forest of Thariyode in Wynad, and Cheriyakanom forest of Thekkadi in Idukki districts of Kerala State were studied. The phanerogamic flore around the sites of soil sample collection in both localities were identified. The total microbial population was studied in relation to the depth of soil. The microbial population was maximum in the top layer and decreased with increase in depth of soil. The total microbial population was higher in Idukki and in both districts, population of bacteria was maximum followed by actinomycetes and fungi. A diversified group of fungi consisting, Mucor, Syncephalastrum, Trichoderma, Microascus, Cunningphamella, Absidia, Aspergillus, Penicillium, Talaromyces, Paecelomyces and Fusarium was present. Three types of actinomycetes viz. Streptomyces sp. with straight sporophores, flexuous sporophores and fascicled sporophores were present while four types of bacteria viz., B. subtilis. Bacillus sp. identical to B. subtilis, Bacillus sp. with small cell and fast growth in NA and Bacillus sp. with small cell and slow growth in NA were present. Antagonistic properties of the isolated were studied with the test organisms Pythium myriotylum, Phytophthora palmivora and Rhizoctonia soleni. Mucor sp. and Cunninchamella elegans showed intermingling and overgrowth with all the test organisms while Absidia corymbefera, Syncephalastrum racemosum, Aspergillus meleus, A. tarreus, Microascus cinereus and Fusarium oxysporum showed this character with P. myriotylum and P. palmivora. Intermingling and overgrowth character was observed in Paecelomyces lilacinus with P. palmivora and R. solani whereas A. versicolor and three species of streptomyces showed this character only with P. palmivora. Mutual inhibition on contact was exhibited by Talaromyces wortmannii with all the three test organisms, while A. versicolor and Streptomyces spp. with flexuous sporophores and fascicled sporophores showed this character with P. myriotylum and R. solani. This character was observed in case of A. corymbefera, S. racemosum, A. meleus, M. cinereus, F. oxysporum and Streptomyces sp. with straigtht sporophores, with R. solani while P. lilacinus showed this with P. myrootylum. Mutual inhibition at a distance was shown by Penicillium citrinum, P. simplicissimum, B. subtilis and the other three Bacillus spp. when tested with P. palmivora and R. solani, but A. terreus showed this reaction only with R. solani. Inhibition at a distance and disintegration of test organism was shown by A. niger with P. myriotylum and R. solani while P. citrinum and P. simplicissimum showed this character only with P. myriotylum. All the three spp. of Trichoderma showed die – back and disintegration of all the three test organisms, while A. niger showed this character only with P. palmivora and A. sydowii showed this character with R. solani only. streptomyces sp. with straight sporophores, B. subtilis and the other three Bacillus spp. showed this character with P. myriotylum alone. Inhibitory properties of antagonists using cell free culture filtrates were estimated and found that A. niger inhibited 100 per cent growth of all the three test organisms while P. citrinum, P. simpliciccimum and B. subtilis showed 100 per cent inhibition of P. myriotylum and a range of 67-87 per cent in case of P. palmovora and R. solani. A. terreus did not inhibit P. myriotylum and P. palmivora, but inhibites 78 per cent of R. solani. All the three Trichoderma spp. moderately inhibited all the three test organisms (13-26 per cent) while A. sydowii showed 20 per cent inhibition of R. solani only. Antibiotic property of the antagonists was determined and P. citrinum exhibited maximum, equivalent to 325 ppm tetracycline followed by Streptomyces with straight sporophores having 250 ppm. T. longibracheatum, P. simplicissimum and A. versicolor recovered antibiotic property equivalent to 150 ppm tetracycline hydrochloride while the other isolates had < 100 ppm equivalence of tetracycline hydrochloride.
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    ThesisItemOpen Access
    Management of sheath blight disease of rice in relation to the population of the pathogen in soil
    (Department of Plant Pathology, College of Horticulture, Vellanikkara, 1987) Shaji, Alexander; KAU; Rajan, K M
    Laboratory, pot and field experiments were carried out to study the role of various physical, chemical and biological factors on the management of sheath blight disease of rice caused by Rhizoctonia solani Kuhn. (Thanatephorus Cucumerlis (Frank) Donk). Populations of total fungi, bacteria and actinomycetes in soil were enumerated at different stages of growth of the crop in order to assess their role in reducing the intensity and incidence of sheath blight disease. Results of the investigation revealed that the pathogen can be managed by deeper ploughing followed by submergence of the soil for a minimum period of two months. Amendments like glyricidia leaves, rice husk, lime and non edible oil cakes of marotti, neem and punna were useful in reducing the severity and spread of the disease. Fungicides like carbendazim and ediphenphos and the herbicide benthiocarb were also efficient in managing the disease. Rotation of rice with crops like tapioca, banana and brinjal was also useful. It was seen that amendments stimulated the population of saprophytes like Trichoderma viride in soil which are antagonistic to R. solani resulting in a subsequent reduction in population of the pathogen. In spite of the reduction in severity and spread of the disease, grain and straw yields were not enhanced by the treatments. More elaborate field trials are to be undertaken before landing on any ultimate conclusion regarding the use of these practices as tools for disease management.
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    ThesisItemOpen Access
    Transmission, properties and hostrance of snake gourd mosaic virus
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1989) Raghunadhan, D; KAU; Rajagopalan B
    Snake gourd mosaic virus disease commonly occurring in Kerala was investigated. The symptoms appeared as typical mosaic mottling with dark green and light green patches. The leaves were much reduced in size, variously crinkled and deformed. Diseased plants were severely stunted and produced only a few flowers and fruits. Transmission studies showed that the virus could be transmitted through mechanical means, grafting and by aphid vectors. The virus was found to be transmitted by the aphids, Aphis gossypii Glov., Aphis malvae Koch. and Aphis craccivora Koch. Among the three species of aphids, A. gossypii was found to be the most efficient vector. Investigations on the physical properties of the virus revealed that the virus had a thermal inactivation point between 70-750C, Dilution end point between 1:10000-1:50000, longevity in vitro between 96-120 h at room temperature (28-300C), and between 144-168 h at 100C. The minimum acquisition feeding and inoculation feeding period were found to be 30 s and 1 min respectively. But the percentage of transmission was maximum when an acquisition feeding of 20 min and inoculation feeding of 1 h were given. Influence of starvation before acquisition and inoculation feeding proved that pre-acquisition starvation for 1 h produced maximum infection but post-acquisition starvation decreased the per cent infection. The vector was found to retain the virus for 1 ½ h. A single aphid could transmit the virus to healthy test plants, but maximum percentage of transmission was obtained with 10 aphids. Host range studies showed that the virus was restricted to the members of the family chenopodiaceae, compositae, cucurbitaceae and solanaceae. Serological studies showed that snake gourd mosaic virus is related to cucumber mosaic virus and pumpkin mosaic virus. Varietal screening trial with seven varieties of snake gourd revealed that all the varieties were susceptible to snake gourd mosaic virus infection. Studies on estimation of loss revealed that early infection reduced significantly the length of vine, number of leaves, number of flowers, number of fruits and yield, whereas late infection did not produce significant effect, on both the varieties tested. There was significant reduction on leaf area and internodal length on plants inoculated at all stages of growth.
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    ThesisItemOpen Access
    Varietal variation for nodulation by Rhizobium in Greengram
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1989) Padmanabhan Nair, V; KAU; Sasikumar Nair
    The present investigation on varietal variation for nodulation by Rhizobium in greengram was conducted at College of Agriculture, Vellayani, Trivandrum. The field trial for host varietal specificity was done at C. S. R. C Karamana of Kerala Agricultural University and in a farmer's plot at Pattom, Trivandrum. Rhizobium capable of nodulation in greengram was isolated from 10 out of 14 different soil samples collected from Alleppey, Quilon and Trivandrum Districts. The ten isolates of Rhizobium obtained initially were screened for their nodulation efficiency under aseptic pot culture conditions by using the greengram variety C0-3. In this screening it was found that the increase was maximum in R5 treatment where 188.30 nodules were formed per plant when compared to only 8.33 in the uninoculated control. The fresh and dry weights of nodules formed 455 and 119.33 mg respectively were also maximum in the R5 treatment. A significant increase in root length was obtained only in two of the above treatments R6 and R9. The Rhizobium culture R5 isolated from C.S.R.C. Karamana, Trivandrum was found superior to all other local isolates of rhizobia. Besides the required minimum increase in dry weight of plants from Rhizobium inoculation, 50 per cent increase in dry mass over uninoculated control (as per the Indian Standard Institution Specifications for Rhizobium inoculants is 0268-1976) was obtained only with this isolate of Rhizobium.
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    ThesisItemOpen Access
    Studies on the seed borne diseases of rice in Kuttanadu
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1986) Jose Joseph; KAU; Susamma Philip
    Several fungi were isolated from the seeds of twelve varieties/cultivars of rice collected from different localities of Kuttanadu, the major rice growing tract of Kerala. Fungi were encountered extrenally as well as internally. Among the externally seed borne fungi, Aspergillus flavus was the most common with its occurrence on nine varieties followed by Bipolaris oryzae and Curvuleria lunata. Other fungi included Rhizopus stolonifer, Chaetomium gracile, Syncephalestrum racemosum, Fusarium moniliforms, Trichoderma virde, Sarocladium oryzae. Aspergillus niger, Alternaria padwickii, Fusarium gramineazum, Piricularia oryzae, A. fumigatus, A. quadrilineatus, Nigrospora oryzae and Penicillium spp. Among the varieties Jaya was found to harbour the maximum fungal population. The germination percentage was not found to be affected due to seed inoculationwith the predominant fungi viz. A. flavus, A. padwickii, B. oryzae, C. lunata and S. oryzae.
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    ThesisItemOpen Access
    Epidemiology and control of sheath rot disease of rice
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1986) Krishnakumaran Nair, B; KAU; Chandrasekharan Nair, M
    Studies on symptomatology, epidemiology and control aspects of sheath rot disease of rice caused by Serocladium oryzae Cams & Hawksworth (= Acrocylindrium oryzae Saw.) were done in detail. The disease initiated as purplish-brown oblong lesions on the sheath of the flag leaf. At maturity lesions enlarged in size with grey -white centres and often covered the entire flag leaf sheath. Panicle often did not emerge but choked inside the affected sheaf itself. Artificial inoculation studies showed that under sufficient humid conditions the pathogen took an average five to eight days for initiation and 15 to 20 days for the complete development of the disease in rice plants. The pathogen was isolated from four naturally infected rice varieties, collected from three different culture (isolates viz., S1, S2, S3, S4, S5, S6 & S7) were selected based mainly on symptomatological variations and their comperative morphological characters were studied in detail. No appreciable differences were noticed in the morphological characters between isolates.
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    ThesisItemOpen Access
    Properties, host-range and control of cowpea mosaic virus
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1987) Sreelakha, L; KAU; Balakrishnan, S
    Studies were conducted on the cowpea mosaic virus disease commonly occurring in cowpea plants (Vigna unguiculata (L.) Walp) in Kerala The major symptoms caused by cowpea mosaic virus infection in cowpea plants were vein banding, inter- veinal chlorosis mosaic mottling and general stunting of the plants. Transmission studies showed that the virus could be transmitted through mechanical means, grafting, through seeds and by means of aphid vectors. The virus was found to be transmitted by the aphids, Aphis craccivora. Aphis gossvpii and Aphis malvae. Among the three species of aphids, Aphis craccivora was found to be the most efficient vector. The percentages of transmission obtained by A. craccivora, A. gossypii and A. malvae were 90,65 and 45 respectively. Studies on the physical properties of the virus revealed that the virus had a thermal inactivation pointbetween 50 and 55°C, dilution end point between 10“"* and —2 10 , longevity in vitro of 8 h at room temperature and24 h at 8°C. The minimum acquisition feeding and inoculation feeding period were found to be 30 s each. But the percentage of transmission was maximum when an acquisition feeding of 10 rain and inoculation feeding of 15 rain were given. Pre-acquisition fasting up to a period of 2 h increased the percentage of transmission, whereas the post-acquisition fasting decreased the efficiency of transmission. The vector was found to retain the virus for 1-2 h. Maximum percentage of transmission was obtained with 10 aphids and the symptoms appeared within 14 days after inoculation. Host-range studies revealed that the virus is restricted to the family Leguminosae and Chenopodiaceae. Varietal screening trial with ten different varieties of cowpea showed that all the ten varieties were susceptible to virus infection. C-152 was found to have the highest susceptibility of 100 per cent infection. C.G.104 showed some resistance with 13.33 per cent infection. In serological studies the cluster bean mosaic virus and sword bean mosaic virus were found to be related to cowpea mosaic virus. The antiserum titre and end point of virus in the present study were found to be between 1;1024 and 1:2048 and 1:512 and 1:1024 respectively. Studies on the effect of virus infection on growth of cowpea plants showed that there was significant reduction in height of the plant, number of pods produced and length of pods. Observations on natural incidence of cowpea mosaic virus indicated that cowpea mosaic virus is the major disease among the different virus diseases affecting t cowpea. Among the infected plants 57 per cent showed cowpea mosaic virus. The results of the experiments to find out the effect of leaf extract sprays on cowpea mosaic virus infection indicated that the disease could be effectively controlled by pre-inoculation sprayings with leaf extracts of Bougainvillea sp. and Eupatorium odoratum.
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    ThesisItemOpen Access
    Studies on the effect of age of plant and host nutrition on bacterial leaf blight of rice and its control
    (Department of Plant Pathology, College of Agriculture, Vellayani, 1985) Sreelatha, K; KAU; Sasikumar Nair
    A study on tho effect of age of plants and host nutrition on the incidence of bacterial leaf blight of rice caused by Xanthomonas camoestris pv oryzae was conducted at the College of Agriculture, Vellayanl, Trivandrum during 1933-84. The field experiment to evaluate the comparative efficacy of different control measures of bacterial leaf blight was laid out in a farmers field at Kainakary in Kuttanad. The effect of age of plants on the incidence of bacterial leaf blight was studied using three popular varieties of rice namely T (N)l Jay a and IR-20 at four critical stages of plant growth ouch as seedling maximum tillering panicle initiation and early flowering stages. At flowering stage the onset of disease symptoms was noticed only on the seventh day while it was observed on the fifth day in all the three varieties for the remaining three stages of plant growth* T(N)1 was found to be the most susceptible variety to bacterial leaf blight followed by Jaya and IR-2Q. Among the four stages maximum tillering stage was the most susceptible stage to bacterial leaf blight. The study on the effect of host nutrition on the incidence of bacterial leaf blight indicated that when higher doses of nitrogen were added, the onset of symptoms were earlier on the fifth day after inoculation with the pathogen while at lower levels# the symptom appeared only on the seventh day. The main effect of nitrogen and potassium on the disease intensity was significant while the effect of phosphorus and minor element was insignificant. The application of nitrogen in general increased the disease intensity while the application of potassium decreased toe same. But at higher levels of nitrogen a similar positive effect of potassium was not observed