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ThesisItem Open Access Genetic divergence, prepotency and inbreeding depression in para rubber (Hevea brasiliensis Muell. Arg.)(Department of Plant Breeding, College of Agriculture, Vellayani, 1992) Kavitha Mydin, K; KAU; Gopinathan Nair, VA study on genetic divergence, prepotency and inbreeding depression in rubber was undertaken in an effort to identify clones for use as components of polyclonal seed gardens. Forty clones of Indian, Indonesian, Malaysian and Sri Lankan origin were evaluated in a replicated trial at the Rubber Research Institute of India. Genetic variability, correlations and the cause and effect relationships of dry rubber yield and its components were worked out. Genetic divergence was estimated employing the Mahalanobis' D2 techinique. The factors of divergence were identified through principal factor analysis. Twenty promising clones from genetically divergent clusters were subjected to seedling progeny analyses for the estimation of propotency based on performance of their open pollinated seedling progenies and inbreeding depression in the first generation of selfing. Significant clonal variation was revealed in respect of all the physiological, morphological and structural attributes studied as mean values for the fourth year of tapping for the stress period and for the peak yield period. High genetic variability for volume of latex under stress, plugging index under stress, annual mean dry rubber yield and dry rubber yield during the stress and peak periods was indicated by the high estimates of genotypic coefficient of variation. Additive gene effects offering scope for improvement through selection was indicated for dry rubber yield, latex flow rate and volume of latex during the three periods, girth increment rate, annual mean plugging index and plugging index under stress, by the moderate to high heritability estimates along with high genetic advance for these traits. Non- additive gene action was indicated by the high heritability and low genetic advance for dry rubber content during the three periods, girth and bark thickness. At both genotypic and phenotypic levels, annual mean dry rubber yield showed moderate to high positive correlations with dry rubber content and latex flow rate during the three periods, girth, girth increment rate, length of the tapping panel and bark thickness and negative correlations with yield depression under stress and plugging index during the three periods. Dry rubber yield under stress emerged as a more important component than peak dry rubber yield by its higher magnitude of positive direct effect on annual mean dry rubber yield. Latex flow rate during the stress and peak periods and annual mean volume of latex exhibited high positive direct effects on annual mean dry rubber yield while plugging index during the peak yield period, volume of latex under stress and girth increment rate had negative direct effects on annual mean dry rubber yield. The magnitude and direction of the effects of the components on dry rubber yield during the three periods varied indicating these relationships to be under different genetic control. Selection for a high dry rubber yield under stress, annual mean volume of latex and latex flow rate during the stress and peak periods and against a high plugging index during the peak period, volume of latex under stress and girth increment rate would help achieve improvement in annual mean dry rubber yield. Considerable genetic diversity was revealed by the wide range of D2 values and intra and inter cluster distances. The forty clones were grouped into eight genetically divergent clusters irrespective of their country of origin indicating the absence of any relationship between geographical diversity and genetic divergence. Volume of latex, plugging index, latex flow rate, dry rubber content and dry rubber yield contributed more towards divergence than the morphological and structural attributes. Supporting evidence was obtained from principal factor analysis which revealed the yield factor to be the main factor of divergence with respect to the clusters studied. Junveile rubber yield on test tapping, number of latex vessel rows and number of leaf flushes in seedling progenies exhibited high heritability and genetic advance indicating scope for their use as early selection parameters, while girth exhibited high heritability and low genetic advance. These three traits showed significant positive correlations with juvenile rubber yield, of which girth exhibited the strongest association. Juvenile rubber yield, number of latex vessel rows, girth and number of leaf flushes were identified as important traits for being accorded simultaneous emphasis in the computation of performance index and index scores for the determination of recovery of superior seedlings as estimates of prepotency. Nine clones were identified as likely preopotents on the basis of seedling progeny analysis at the age of two years. Selfing resulted in a lower fruit set than open pollination in the clones in general. No significant inbreeding depression was recorded for juvenile vegetative traits and rubber yield in seedlings. Clones PB 28/83, PB 215, RRII 105, AVT 73, PB 217, PB 252, Ch 26, PB 242 and PB 5/51 were identified as likely prepotents from three genetically divergent clusters. They recorded superiority for yield and various yield components. These clones exhibited synchrony in flowering and are suggested as components of a nine parent polyclonal seed garden. For a seven parent seed garden the clones suggested to be excluded are PB 5/51 and PB 242. A polyclonal seed garden comprising these nine or seven clones as components could generate good quality polycross seed material. Appropriate seed garden layouts have been suggested.ThesisItem Open Access Genetic analysis of biological nitrogen fixation traits and yield components in cowpea (Vigna unguiculata (Linn). walp)(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 1995) Sreekumar, K; KAU; Manikantan Nair, PA study on the parameters of variability, correlations, path-coefficients, combining ability, gene action and heterosis in cowpea was undertaken at the Department of Plant Breeding and Genetics, College of Agriculture, Vellayani during 1991 to 1993. Fifty three genotypes of cowpea collected from different sources were planted in a field experiment for the estimation of variability, correlation and path coefficient. Eleven biological nitrogen fixation characters, viz., number of days to flower, length of primary root, number of secondary roots, number of nodules in the primary root, number of nodules in the secondary roots, total number of nodules, weight of effective nodules in the primary root, weight of nodules in the secondary roots, total weight of nodules, nitrogen content in the plant at 50 per cent flowering and plant dry weight and six yield characters, viz., grain yield per plant, length of pods, number of pods per plant, number of seeds per pod, weight of 100 seeds and seed protein content were considered for this study. The ten selected varieties/types from the initial evaluation trial were crossed in a line x tester model, keeping the three high nitrogen fixing types and three high yielding types as lines (total six lines) and two low nitrogen fixing and two low yielding types as testers (total four testers). The F1’s along with their parents were compared in a field experiment and combining ability, gene action and heterosis were estimated. The study of combining ability and gene action were confined to six biological nitrogen fixation characters, viz., number of days to 50 per cent flowering, weight of nodules in the primary root, total weight of nodules, weight of effective nodules, dry weight of the plant and nitrogen content per plant and six yield characters, viz., length of pod, number of seeds per pod, number of pods per plant, hundred seed weight, seed protein content and grain yield per plant. The analysis of variance revealed that a considerable amount of variation among the varieties was present with respect to the characters under study. Characters like number of days to flower, total nodule weight, nitrogen content per plant, weight of 100 seeds and seed protein content had recorded narrow relative magnitude of difference of phenotypic and genotypic coefficient of variation along with high heritability estimate. Moderate magnitude of difference of PCV and GCV along with moderate heritability was recorded for the characters viz., number of nodules in the primary root, number of nodules in the secondary roots, total number of nodules, weight of effective nodules in the primary root, weight nodules in the secondary root, length of pods and number of pods per plant. Plant dry weight and grain yield registered a wider difference of PCV and GCV along with low heritability indication the greater influence of environment over these two characters. Genetic advance as percentage of mean was found to be high for the characters like number of nodules in the secondary roots, weight of effective nodules in the primary root, weight of nodules in the secondary roots, total nodule weight, number of pods per plant and 100 seed weight and moderate for number of nodules in the primary root, total number of nodules and grain yield. Low genetic advance was recorded by number of days to flower, plant dry weight, nitrogen content per plant, length of pod and seed protein content. Hence characters such as number and weight of nodules in the primary root, number and weight of nodules in the secondary roots, total number and weight of nodules, number of pods per plant and weight of 100 seeds may be controlled by additive genes whereas days to 50 per cent flowering, nitrogen content in plant, length of pod and seed protein content may be controlled by non-additive genes. Correlation coefficients were workedout at the genotypic and phenotypic levels. Based on the genetic correlation of characters studied, it was understood that high nitrogen fixing genotypes may not be high yielders because of the antagonistic relationship between grain yield and total nitrogen per plant. Weight of nodules in the primary root and total nodule weight were positively correlated with the nitrogen content in plant. Hence genotypes which was able to form effective large nodules on the primary root system seems to be a better nitrogen fixer. Number of days to 50 per cent flowering had negative genotypic correlation with grain yield. Hence an early flowering genotype may be better yielder than a late flowering type. Weight of hundred seeds and seed protein content exhibited very strong negative correlation indicating that small seeded genotypes may be better with respect to protein content. Grain yield recorded positive phenotypic and genotypic correlation with number of pods per plant. Path coefficient analysis at the genotypic level revealed that total number of nodules had the highest positive direct effect on nitrogen content per plant followed by weight of effective nodules in the primary root and weight of effective nodules in the secondary root. Highest positive direct effect was recorded for length of pod with grain yield. The combining ability analysis revealed that both additive and non-additive gene actions were important for all the characters under study. However GCA and SCA variance ratio which was less than unity for all the traits under study indicated the predominance of non-additive gene action in the inheritance of these traits. Considering the combining ability effects, VCP 4 was found to be the best general combiner for most of the biological nitrogen fixing characters and V 322 was the best general combiner for the grain yield. The cross combination of V 27 x C 152 and V 271 x Co Vu 85020 showed the best performance with respect to sea for the character number of days to 50 per cent flowering while VCP 4 x C 152 for weight of nodules in the primary root and nitrogen content in plant. DPLC 210 x PTB 2 recorded high sea for total weight of nodules, weight of effective nodules, dry weight of the plant and number of pods per plant, on the other hand Co Vu 358 x C 190 recorded high sca for length of pod, number of seeds per pod and seed protein content. The cross combination V 322 x C 190 exhibited high sca for hundred seed weight and V 27 X C 152 for grain yield per plant. Marked heterosis was observed in many cross combinations for most of the characters studied and pronounced heterotic expression was obtained for weight of nodules in the primary root, total weight of nodules, weight of effective nodules, dry weight of the plant at 50 per cent flowering, number of pods per plant and grain yield. It was already established that these characters are predominantly governed by the non – additive gene action. Hence the heterotic vigour expressed by the hybrid combination with respect to these characters are justified. Since the biological feasibility for the exploitation of heterosis is not economical as a plant improvement programme in this crop, genetic improvement of these trait can be brought about more effectively through combination breeding involving genetically diverse and high combining parants.ThesisItem Open Access Induction of genetic recombinations in interspecific crosses of Abelmoschus(Department of Plant Breeding and Genetics, College of Agriculture, Vellayani, 1994) Sheela, M N; KAU; Manikantan, Nair PA study was undertaken at the College of Agriculture, Vellayani during 1990-91 aimed at inducing recombinations of the economic attributes of Bhindi (Abelmoschus esculentus (L.) Moench) and the yellow vein mosaic disease resistance of wild relatives. A preliminary evaluation of 56 accessions revealed good genetic diversity in Bhindi germplasm. The accessions were grouped into four clusters. The characterization of germplasm was done based on IBPGR descriptors. High genotypic coefficients of variation were exhibited by weight of fruits per plant, leaf area, height of plant, number of fruits per plant, single fruit weight and number of branches per plant indicating scope for selection. High heritability along with high genetic advance was recorded for weight of fruits per plant, height of plant, leaf area and number of seeds per fruit. Low heritability coupled with low genetic advance recorded for yellow vein mosaic disease incidence indicated the predominant role of environment in the inheritance of disease resistance.ThesisItem Open Access Fruit component and seedling progeny analysis of komadan coconut types(Department of Plant Breeding, College of Agriculture, Vellayani, 1992) Manju, P; KAU; Gopimony, RThe present study was undertaken with a view to unravel the genetic status of a coconut type, Komadan, popular in the Central Travancore region of Kerala, in relation to its origin by comparing its three generations with WCT and NCD through fruit component and seedling progeny analysis. The study revealed that the Komadan types showed significant superiority for majority of the mother palm characters especially number of bunches and spadices and number of nuts per palm per year. Number of nuts per palm per year and number of female flowers per bunch had high heritability combined with moderate to high genetic advance indicating the predominance of additive genes. Among the Komadan palms, 33 per cent were of self pollinating nature thereby occupying a position in between WCT and NCD regarding pollination system. All the Komadan palms had nuts of different shades of brown while 70 per cent of WCT palm had nuts of green shade and NCD palms had varying shades of green, olive and brown nuts indicating the distinction of Komadan as a separate group. Komadan types were superior to WCT in all seednut characters except polar diameter of nut, oil content, thickness of husk and husk/nut ratio. It behaved as a distinct type regarding thickness of meat. One or the other of the Komadan generations was found to be on par with NCD for majority of the seednut characters. Komadan types occupied an area midway between NCD and WCT based on Niu kafa-Niu vai Introgression hypothesis with a clear progression through generations towards better weight of unhusked nut. Inbreeding depression was noticed in WCT and NCD as indicated by significant reduction in embryo and kernel weight in selfed nuts compared to open pollinated nuts. In Komadan there was no inbreeding depression indicating a high degree of homozygosity. The seednuts of Komadan types showed early germination compared to WCT and NCD. Regarding seedling characters, Komadan behaved as a distinct type for total leaf area over both WCT and NCD. Number of seedlings with split leaves was also more in Komadan. This type was superior to WCT for seedling vigour index also. Estimation of prepotency based on recovery of quality seedlings showed that 40 to 60 per cent of Komadan palms were prepotent where as the same in WCT and NCD were only about 20 per cent. Analysis of petiole colour of seedlings showed that 71 to 82 per cent of seedlings among the three Komadan generations showed moderates brown colour (bronze) ; 97 per cent of WCT seedlings had different shades of green and wider variations in petiole colour were noticed in NCD seedlings. It can be concluded that the Komadan type maintained its genetic identity over generations with respect to economically important characters viz. Kernel, copra, and oil content and also in the recovery of quality seedlings which inturn reflects its prepotent nature. The collection and maintenance of typical komadan germplasm through inter se crossing and development of isolated seed gardens for large scale production of komadan seedlings are suggested as future line of work.
