Thumbnail Image

Theses

Browse

2010 - 20123
Reset filters
Subject: Others × End date: 2012 × Start date: 2010 ×
Reset filters

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    ThesisItemOpen Access
    Identification of paprika (Capsicum annuum L) genotype(s) for yield and quality characters
    (Department of Olericulture, College of Agriculture,Vellayani, 2012) Lekshmi, S L; KAU; Sreelathakumary, I
    The experiment entitled “Identification of paprika (Capsicum annuum L.) genotype(s) for yield and quality characters” was conducted at the Department of Olericultue, College of Agriculture, Vellayani, during the period 2011-2012. The study envisaged assessment of genetic variability in paprika and to study the influence of harvest maturity on quality parameters. Fifty three accessions of paprika were collected from different parts of country and grown in the field in RBD with three replications. The analysis of variance revealed highly significant differences among the 53 accessions of paprika for all the characters studied viz., plant height, primary branches, days to flowering, days to maturity, node to first flower, height of node to first flower, fruit length, fruit girth, fruit weight, fruits per plant, yield per plant, pedicel length, fruit: pedicel ratio, flesh thickness, seeds per fruit, flesh: seed ratio, driage, oleoresin, colour, ascorbic acid, capsaicin, bacterial wilt and leaf curl virus incidence. Among the accessions CA 6 recorded highest yield per plant (776.12 g) and CA 12 recorded maximum number of fruits (265.33). Fruit weight was highest in CA 47 (13.43 g). High phenotypic coefficient of variation and genotypic coefficient of variation were observed for yield per plant, fruits per plant, fruit weight and primary branches per plant. High heritability and high genetic advance also observed for these characters. The path analysis revealed that fruit weight, fruits per plant, plant height and primary branches had direct effect on yield per plant. Correlation and path analysis revealed that fruits per plant is the primary component as evident from the positive correlation as well as high direct and indirect effect on yield. Maximum oleoresin content was observed in CA 7 followed by CA 29 and CA 37. CA 2 recorded a high color value with pungency and CA 37 recorded a high colour with low pungency. CA 38 recorded maximum ascorbic acid content. CA 34 and CA 40 had the minimum pungency and CA 10 recorded the maximum pungency. Bacterial wilt and leaf curl virus incidence among the 53 accessions were studied. CA 33, CA 34, CA 35 and CA 47 recorded less incidence of both diseases. Based on Mahalanobis D2 analysis the current genotypes were grouped into four clusters. Cluster I was the largest with 34 accessions, followed by cluster IV with 15 accessions and cluster II with three accessions. Cluster III had only one accession. In the present study maximum divergence was observed between clusters II and IV as shown by their high intercluster distance. The minimum intercluster distance observed between the clusters I and II indicated a close relationship among the accessions. Based on selection index including both quantitative and qualitative characters CA 34 (Local, Dharwad) was ranked first followed by CA 7, CA 6, CA 33 and CA 35. The accessions were genetically catalogued and the result revealed distinct variations among the accessions for vegetative, inflorescence, fruit, seed and quality characters. Quality characters showed significant differences among the accessions and harvest maturity stages. Oleoresin, colour and capsaicin content in the paprika fruits were found to increase as the age of the fruits increased. Ascorbic acid content in the fruits increased from turning stage to red ripe and then declined. On the basis of the present study the high yielding accessions can be grouped into low pungent and pungent paprika. Among the low pungent, high yielding accessions CA 34, CA 33 and CA 35 recorded high colour value also. CA 6 and CA 7 are promising high yielding pungent paprika accessions. These accessions can be utilized for further crop improvement programmes.
  • Thumbnail Image
    ThesisItemOpen Access
    Formulation of low fat beef burger with fat replacers
    (Department of Livestock Products Technology, College of Veterinary and Animal Sciences,Mannuthy, 2010) Govande Premanand Lxmanrao, KAU; George Oommen, T
    Health conscious meat consumers prefer low fat meat products due to increasing incidents of high fat induced diseases. Manufacturing meat products with fat replacers (FR) enable to reduce fat and to alleviate the problems with the reduction of fat in products. Therefore, the present study was undertaken with the objectives of developing a palatable and economic formulary for low fat beef burger (LFBB) with carrageenan (CG), tapioca starch (TS), pregelatinised pork skin collagen (PSC) and their blends as FR and to assess its pH, cooking characteristics, proximate composition, nutritional value, textural and organoleptic qualities and shelf life under aerobic (AP) and vacuum packaging (VP) at 0-4oC and -20oC and its cost of production. Beef burgers (BB) are formulated at two different fat levels, viz., full fat (FF) 20 per cent and low fat (LF) 5 per cent as controls. Seven formulations of LFBB with 5 per cent fat are prepared with 0.5 per cent CG, 1.5 per cent TS, 2 per cent PSC and their blends, viz., CG-TS - 0.5% CG & 1.5% TS; CG-PSC - 0.5% CG & 2.0% PSC; TS-PSC - 1.5% TS & 2.0% PSC; CG-TS-PSC - 0.5% CG, 1.5% TS & 2.0% PSC as FR. BB are prepared as per the formularies with minced lean beef trimmings, tallow, salt, spices and condiments, rusk, ice flakes and FR. They are packaged aerobically in HDPE and in vacuum in polyethylene-polyamide (PEPA) pouches. pH, cook yield (CY), cook loss (CL), fat retention percentage (FRP), moisture retention percentage (MRP), dimensional shrinkage (DS), water holding capacity (WHC), Warner-Bratzler Shear Force (WBSF), Hunter L*, a*, b* colour values, proximate and mineral composition and nutritional value, purge loss (PL), Thiobarbituric Acid Reactive Substances (TBARS) value and sensory qualities are assessed on d 0, 10, 20 and 30 of storage at 0-4oC and -20oC or till spoilage, whichever is earlier. Six trials of the experiment were conducted. Cooking reduced the acidity of all the burgers. By the addition of FR a significantly (P< 0.05) very low acid cooked LFBB could be prepared. CY of burgers with CG-TS-PSC was significantly (P< 0.05) the highest with 85.84 per cent. LFBB with blends of FR significantly (P< 0.05) increased CY and correspondingly reduced CL. The DS in LFBB with CG-TS-PSC was significantly (P< 0.05) the lowest with 13.21 per cent. Addition of blends of FR holds water and fat in LFBB and reduces DS during cooking. FRP and MRP in CG-TS-PSC formulation was significantly (P< 0.05) the highest with 97.66 and 74.36 per cent, respectively due to blends of CG, TS and PSC. The WHC of LFBB with CG-TS-PSC was 95.36 per cent and WBSF value 5.30 N comparable to FF and the burgers were significantly (P< 0.05) most succulent, juicy and tender with the addition of blends of FR compared to tougher BB without FR. According to Hunter L*, a*, b* values, LFBB with blends of FR, especially CG-TS-PSC was lighter, less reddish (more bluish) and less yellowish (more greenish) and comparable to FF burger. Fat content in the beef trimmings and PSC were < 1.76 per cent. Cooking significantly (P< 0.05) reduced moisture content with a corresponding increase in the protein, fat, carbohydrate and ash. The percentage total calorific value of LFBB ranged from 6.36 to 7.18 of the Recommended Dietary Allowance (RDA). The contribution of fat to RDA of calorific value was from 2.22 to 2.42 per cent only, which was below the recommended 30 per cent. More than one third of the daily requirement of protein is obtained from 100g of LFBB. LFBB with FR are good sources of Na, K and P but not of Ca. Blends of FR in LFBB, especially CG-TS-PSC, were more efficient in significantly (P< 0.05) reducing PL and TBARS value on storage at 0-4oC for 10 days and at -20oC for 30 days in AP and VP. TBARS values were lower than the acceptable range of 1mg malonaldehyde/kg for oxidative rancidity. The low fat content and the presence of onion containing antioxidants in the formulary would have synergistically acted with CG in reducing the TBARS. On sensory evaluation on zero day, the LFBB with CG-TS-PSC scored significantly higher (P< 0.05) values of 7.00 and above for very good appearance and colour, very intense flavour, very desirable texture, juiciness, practically nil mouth coating and very acceptable overall acceptability similar to FF burger. But saltiness was very desirable than in FF. The LFBB with CG-TS-PSC in AP and VP retained all the sensory attributes and proximate composition even on storage. The very acceptable nature of CG-TS-PSC formulation might be due to the synergistic effect of fat replacers. The LFBB with 5 per cent fat and CG (0.5%), TS (1.5%), PSC (2%) and their blends as FR are developed economically with very acceptable overall acceptability, CY, nutritional quality, reduced PL and oxidative rancidity and shelf life up to 10 days at 0-4oC and 30 days at -20oC under AP and VP. The best LFBB with overall acceptability was CG-TS-PSC followed by CG-TS, CG-PSC, TS-PSC, PSC, CG and TS. Blends of FR are better than single FR, particularly CG-TS-PSC, as they increased CY, FRP, MRP, WHC, sensory attributes and decreased pH, CL, DS, WBSF, PL and TBARS. Further investigations with production of large quantities are required for calculation of cost of production at commercial scale.
  • Thumbnail Image
    ThesisItemOpen Access
    Studies on the ameliorative effect of vitamin E on paraquat toxicity in rats
    (Centre for Excellence in Pathology, College of Veterinary and Animal Sciences,Mannuthy, 2010) Divya, V S; Vijayan, N
    The present study entitled ‘Studies on the ameliorative effect of vitamin E on paraquat toxicity in rats’ was undertaken by administering the animals with paraquat dichloride and vitamin E for a period of 28 days. The weekly body weights, clinical signs, haematology and biochemical parameters were analysed. Of the twelve animals in each group, six animals were sacrificed at the end of second week and remaining at the end of forth week. The gross pathology and histopathology of various organs were studied. The oxidative damage of the lungs and kidneys was assessed by the estimation of lipid peroxides and reduced glutathione. None of the animals in the experimental groups revealed any clinical signs of toxicity except for one rat each from paraquat alone treated group and paraquat plus vitamin E treated group which became weak towards the end of experiment. Although, all the groups showed a gradual increase in body weight, those animals treated with paraquat alone and those with paraquat and vitamin E showed a reduced weight gain when compared with the control group. The AST, ALT, ALP and creatinine values showed a significant increase in treatment groups compared to the control. In vitamin E treated group, a slight decrease was noticed in these biochemical parameters. Hb and PCV values showed a significant decrease and the total leukocyte count showed a significant increase in treatment groups when compared to control group. There was no variation in the erythrocyte sedimentation rate and differential leukocyte count. In vitamin E treated group, a slight increase in Hb and PCV values were noticed. There was an increase in the lipid peroxides and concurrent reduction in the glutathione in the lung and kidney homogenate which indicated oxidative damage. Gross lesions were almost similar in both treatment groups. The congestive changes were noticed in lungs, kidneys and liver. The histopathological changes noticed in paraquat alone treated group were congestion, haemorrhage, focal areas of necrosis with proliferation of fibroblasts in the lungs, thickening of the interalveolar septa. The bronchi revealed desquamation of proliferated epithelium. Degenerative changes in the renal tubules, focal necrotic changes in the liver and varying degrees of degenerative changes in the heart, stomach, intestine and brain were the other lesions noticed. These lesions became more intensified in animals treated for four weeks. In vitamin E treated group, a slight reduction in the intensity of lesion was noticed. The bronchial epithelium was intact. Mild proliferation of fibroblasts and congestive changes were also noticed in the lungs. Focal necrotic changes were noticed in the kidneys and liver. Mild degenerative changes were noticed in the heart and intestine. The study revealed that the vitamin E is not completely suitable for management of the renal and pulmonary disorders in subacute paraquat toxicity in rats.