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ThesisItem Open Access Shelf life of irradiated rabbit meat underaerobic and vacuum packaging(Department of Livestock Products Technology, College of Veterinary and Animal Sciences, Mannuthy, 2009) Sonika, S; KAU; Kuttinarayanan, PPrevention of Food Adulteration Act (1954) as amended in 1998 has permitted irradiation at a dose rate of 2.5 to 4.5 kGy to control pathogenic microorganisms and to extend the shelf life of meat and meat products including poultry products. A study was conducted to evaluate the shelf life of irradiated rabbit meat under aerobic and vacuum packaging. The rabbit meat was prepared by slaughtering locally purchased rabbits under hygienic conditions and was packed in HDPE and PAPE packages at a rate of 120 g each. Half of the packets of aerobic and vacuum packaged samples were subjected to gamma radiation at 2.5 kGy at melting ice temperature and kept immediately at chiller temperature (1 to 4oC) and domestic refrigerator freezer (-6 to -8oC). Samples were analyzed for physical, physicochemical, microbiological, and organoleptic qualities on the day of preparation and on days 3, 5, 10, 15, 20, 25, 30, 40, 45, 50, 60, and 70 of storage or until spoilage, whichever was earlier. The samples were also analyzed for proximate composition on the day of preparation. The dressing percentage of the rabbits subjected to the study was 49.35 per cent. The keeping quality of the rabbit meat was 15 to 18, 17 to 19, 5 to 7 and 7 to 9 days in HDPE IR, PAPE IR, HDPE NR and PAPE NR respectively at chiller temperature. In freezer temperature it was significantly (P < 0.05) increased to 45 to 47, 47 to 49, 25 to 27 and 27 to 29 days in HDPE IR, PAPE IR, HDPE NR and PAPE NR respectively. Irradiation or packaging did not significantly (P < 0.05) affect fat, protein and ash composition but higher moisture percentage was observed in irradiated samples. Swelling of the collagen fibres was noticed in irradiated samples on histological examination. The physicochemical parameter, pH of irradiated and non-irradiated samples varied significantly (P < 0.05) on the day of preparation. On storage the pH values decreased uniformly. The WHC was significantly (P < 0.05) reduced in irradiated samples. The ability of the rabbit meat to retain its water decreased gradually on storage and a reduction of about 40 per cent could be noticed from the initial level. Drip loss was not significantly (P < 0.05) different on the day of preparation. Irradiation had a significant (P < 0.05) effect in enhancing the cooking loss whereas packaging had little effect. Both drip loss and cooking loss was increased significantly (P < 0.05) due to storage under chiller and freezer temperature. Irradiation had a significant (P < 0.05) role in increasing the TBARS value of rabbit meat. As the days of storage enhanced, the TBARS values were increased. Irradiation had a significant (P < 0.05) effect in reducing the TV in both type of packaging. As storage period enhanced, TV increased with significant (P < 0.05) changes among treatments. Irradiation had a beneficial effect on microbiological qualities of rabbit meat. There was a significant (P < 0.05) reduction of nearly three log in APC of irradiated meat from that of control. The irradiation of the samples both in HDPE and PAPE packaging significantly (P < 0.05) reduced the PC of meat on the day of preparation where as storage had significant (P < 0.05) effect in enhancing the microbial load of meat. The colour score was non-significantly (P < 0.05) higher in irradiated samples. The juiciness, tenderness and overall acceptability scores were improved significantly (P < 0.05) due to irradiation, where as flavour score was reduced. The sensory attributes were significantly (P < 0.05) reduced due to storage in all the treatment groups. The keeping quality of rabbit meat was significantly (P < 0.05) increased by irradiation both in chiller and freezer under different packaging. In addition irradiation could effectively control food borne illness by destroying the major pathogenic organism without affecting the sensory and nutritional quality of the product. Considering these advantages it can be recommended that packaging the meat in PAPE packages followed by low dose gamma irradiation and maintaining the cold-chain contribute to extended storage life of rabbit meat.ThesisItem Open Access Waste management system evaluation in commercial dairy farms(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2009) Sany Thomas; KAU; Joseph MathewThe present study on “Waste management system evaluation in commercial dairy farms” was conducted to assess the usefulness of the different waste management methods adopted in dairy farms of Kerala. Study area comprised of Thrissur, Malappuram and Ernakulam districts and adjoining area of central and northern Kerala. Forty five dairy farms were identified and visited in and their profile of the study area .Data regarding general outlay of the farms selected management practices in the farm, livestock details and existing waste management methods in the farms were collected and studied. The dairy farms under study were classified based on the animal holding capacity as those with less than six animals (class 1), 6-20 animals (class 2), 21-50 animals (class 3)and 51-100 animals (class 4), and above 100 animals (class 5). Among the 45 farms under study, four per cent belonged to class 1, forty nine per cent belonged to class 2, thirty five belonged to class 3, six per cent farms came under the classes 4 and 5.There was no commercialization of waste management methods adopted by the farms. The majority farms had dung pit as waste disposal method and no regularity in dung removal from pit, it was upon demand. Regarding the soil quality, there was no significant difference in the soil nutrients quantity between different groups of farms. Regarding water quality, the water samples collected from the nearby water bodies were inferior in quality in terms of microbiology and BOD. Air quality stands below the permitted standards in all groups of farms. Intervention by the government, local bodies and scientific institutions must be made in this area supporting the farmers to develop awareness in this serious issue and also providing necessary technical and financial support for the farmers to construct proper waste management systems such as compost unit as well as biogas plants because they provide a support for the most efficient waste management system in a dairy farm proving that waste is wealthThesisItem Open Access Management protocol for averting drop of milk production in dairy cattle during summer(Department of Livestock Production Management, College of Veterinary and Animal Sciences, Mannuthy, 2009) Nisanth, P; KAU; Kannan, AA study was conducted to assess the effect of feeding management on milk production and metabolic profile of crossbred cows in mid lactation. Twelve healthy crossbred cows having a peak yield of minimum eight litres in the previous lactation, were selected as the experimental animals. The animals were divided into two groups of six each as uniformly as possible with regard to age, milk yield and parity and were randomly allotted to two dietary treatments, T1 consisting of basal concentrate mixture and green grass as roughages and T2 consisting basal concentrate mixture, green grass as roughage of which 1/3rd of the concentrate and roughage is fed during the day time and rest in the evening and early morning with water availability at all times. Daily maximum temperature, minimum temperature and relative humidity were recorded inside the shed using maximum and minimum thermometer to quantify the microenvironment prevalent around the animals in both the treatment groups. Physiological parameters like respiration rate and rectal temperature were measured to give an immediate response to the climatic stress and consequently the level of comfort to the animal. Individual records of daily intakes of concentrate and roughage, daily milk production and fortnightly body weight were maintained through out the experiment. Milk samples were collected at fortnightly intervals and were analysed for milk fat, total solids and solids not fat. Blood was collected at fortnight intervals for estimating haemoglobin, erythrocyte sedimentation rate, packed cell volume, plasma glucose and cortisol. The ambient temperature prevailed during the study period was higher than what was reported as comfortable or ideal temperature for better livestock production and the excess humidity and temperature levels recorded in the present study at Mannuthy could be classified as humid and hot. The average rectal temperature during morning and afternoon between T1 and T2 did not differ significantly. The mean values clearly indicated that high ambient temperature increased the respiratory rate in the afternoon. The respiration rate for morning and afternoon recording was not found to be differed between T1 and T2. Average body weight of animals revealed no significant difference for the both groups during all the fortnight studied. The average daily dry matter intake linearly increased as the lactation progressed in both the groups. Dry matter intake by the animals of T2 was comparatively better than the animals of T1 with a significant increase (P<0.05) during the fourth and seventh fortnight. Scrutiny of the data on milk production during first 120 days of lactation further revealed that comparatively higher milk yield and better persistency was observed in T2. There was no significant difference in any of the milk composition parameters between the treatments. The haematological parameters such as haemoglobin, packed cell volume, erythrocyte sedimentation rate and plasma glucose estimated at the fortnight intervals were not significantly affected by the two dietary treatments. On statistical analysis a significant difference (P< 0.05) in plasma cortisol was obtained between the two groups during the first and second fortnights. The overall average plasma cortisol level was significantly higher in the T1 in comparison to T2. From the overall results obtained in the present study it could be concluded that the night time feeding of the animals during summer season has improved total milk production and helped to maintain the higher milk yield as well as persistency of milk production in lactating crossbred cows and found that there was some long-term effect on performance of evening fed cows.