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Open Access
Standrdisation of spacing for tissue culture banana cv. nendran (AAB group)
(Department of Horticulture, College of Agriculture, Vellayani, 1994) Anil, B K; KAU; Jayachandran Nair, C S
The investigation on “Standardisation of spacing for tissue culture banana cv. Nendran (AAB group)” was conducted at the Department of Horticulture, College of Agriculture, Vellayani, Thiruvananthapuram during, 1992-’93 inorder to study the effect of different spacings on growth, yield and fruit quality of Nendran banana. The results obtained are presented below: In the early stages of growth, the vegetative characters were not influenced by the spacings tried. However, during later periods, the plant height, girth, number of leaves per plant, total and functional leaf area, interval of leaf production, LAI and LAD increased with decrease in spacing. The time taken for bunch emergence, maturity and duration of crop increased with decrease in spacing. The number of suckers per plant decreased with decrease in spacing, while total number of suckers per hectare increased with decrease in spacing. The biomass and drymatter production per plant, bunch yield and fruit size were higher in wider spacing, while on per hectare basis it was the reverse. Drymatter content, TSS, reducing, non-reducing and total sugars and sugar/acid ratio of fruits increased with increase in spacing, while acidity and ascorbic acid content decreased. The time taken for ripening of fruits decreased with increase in spacing, while shelf life remained unaffected. The benefit/cost ratio was most favourable in 1.75 x 1.75 m spacing. Incidence of pests and diseases were severe in closer spacings above 1.75 x 1.75 m. Soil nutrient depletion was tolerable upto 1.75 x 1.75 m spacing, while uptake and partitioning of major nutrients by individual plants decreased with decrease in spacing. Fruits showed the highest nitrogen and phosphorus content followed by leaf, leaf sheath, corn and pseudostem. Potassium content was the highest in leaf followed by fruits, corm, leaf sheath and pseudostem. In general spacing 1.75 x 1.75 m did not significantly affect the plant growth, yield and quality of fruits. However, the experiment has to be repeated to arrive at conclusive results.
Open Access
Evaluation of nendran (musa aab group) ecotypem
(Department of Horticulture, College of Agriculture, Vellayani, 1996) Bindu Viveka, Devi; KAU; Jayachandran Nair, C S
The investigation "Evaluation of 'Nendran' (Musa AAB group) ecotypes" was conducted at the Department of Horticulture, College of Agriculture, V e l l a y a n i , Thiruvananthapuram during 1994-'95 inorder to study the effect of ecotype variation on growth, yield and fruit quality of 'Nendran' banana. The results obtained are presented below : Vegetative characters like plant height, girth at later stages, number of leaves per plant, phylacron at early stages, leaf longevity, leaf area duration, leaf area index at later stages and monthly growth rate at early stages showed variation among the different ecotypes. The number of suckers per plant were almost uniform for all the ecotypes. The time taken for flowering and total crop duration varied with ecotypes but the maturity period of the bunches was uniform for all the ecotypes. Eventhough bunch yield was maximum in Kaliethan, the number of hands and fingers per bunch were the lowest in this type. Fruit characters like finger length, finger weight, peel weight and pulp weight were high in Kaliethan where as the pulp/peel ratio was high in Poovanchira. Chengazhikodan and Kothala were superior in fruit quality compared to Kaliethan. The leaf nutrient status at flowering stage varied only in the case of potassium where as both phosphorus and potassium varied at harvest stage. Poovanchira, Puthur and Kothala types had higher levels of NPK at flowering time though Chengazhikodan had the highest potassium content. During harvest stage, Kothala and Chengazhikodan had higher NPK content compared to others types. The dry matter production was higher in Kaliethan and Pandaloor in both vegetative part and fruits. The extent of sigatoka leaf spot did not differ significantly among the 'Nendran' ecotypes during the different stages of growth. There was no incidence of bunchy top in any of the treatment plants. Nematode infestation in root was low in Poovanchira, Kaliethan and Chengazhikodan and high in Muttathukonam and Kothala types where as the rhizome weevil incidence was low in Puthur, Kothala and Kaliethan an 1 high in Chengazhikodan and Pandaloor types. Correlation and path analysis studies in 'Nendran' ecotypes indicated that leaf area duration (LAD), girth • fingers, time taken for flowering, number of fingers re bunch, plant height at post floral initiation stage and g i r t h of plant at floral initiation stage had a p o s i t i v correlation with bunch weight. So these characters can 1 -? considered for selecting superior ecotypes. In general, Kaliethan can be considered as the most suitable ecotype of 'Nendran' for commercial cultivation in Thiruvananthapuram and nearby areas. However, the o'h'i types such as Kothala, Chengazhikodan and Poovanchira typos can also perform well in this tract once they become adapt '1’ to the agroclimatic conditions of the zone.
Open Access
Standardisation of in vitro techniques for rapid multiplication of holostemma annulare k schum
(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1996) Sophia John, A; KAU; Kesavachandran, R
Studies were conducted on standardization of in vitro techniques for the rapid multiplication of Holostemma annulare K. Schum. At the Plant Tissue Culture Laboratory of the Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara during 1993-1995. Surface sterilization was standardized for explants from different sources. For two to three month old explants from the glasshouse, treatment with 0.1 per cent mercuric chloride for 5 min or 10 min was found to be better. A combination of sterilants was necessary for mature explants taken either from the glasshouse or field. Explants collected in the months of January and February gave the lowest contamination rate. Early release of buds and further growth of nodal segments and shoot tip explants was better in MS media supplemented with BA. Cultures in medium containing KIN were short, robust, darker and with lesser number of buds and shoots than those in medium containing BA. Extremely low concentrations of TDZ could stimulate axillary bud proliferation. Additives like silver nitrate and activated charcoal could drastically reduce callus production in culture, but the shoot growth was also reduced with these additives. Nodal segments were better in respect of early release of buds, more number of longer shoots, nodes and buds than shoot tips. Higher temperature proved better than lower temperature for the growth of cultures. Also exposure to light was favourable for healthy growth of shoots. Proliferation rate was higher at higher concentrations of BA but the shoots were very swollen, weak and had to be subcultured as a clump into media containing lower concentrations of BA for healthy growth of shoots. Shoots could be proliferated at extremely low concentrations of TDZ. MS basal with full concentration of salts was better for better growth of shoots. When the best treatment in each subculture was given in sequence approximately 2 crores 37 lakh nodes could potentially be obtained over a period of 225 days. Maximum rooting, early rooting and more number of longer roots could be obtained in solid. MS basal media when shoots were kept for in vitro rooting. Ex vitro rooting of shoots was successful when they were treated with IBA 1000 mg1-1 as quick dip followed by planting in plastic pots filled with sand in the initial stages for early rooting and then transplanted to plastic or mud pots filled with cocofibe for vigorous growth of root and shoot portions. TDZ produced the highest callus index at relatively lower concentrations. The callus produced was hard, green in colour and compact. 2, 4-D proved better than NAA for obtaining more regenerative callus among the auxins tried. Leaf segments (with or without petiole attached) oriented with the abaxial surface touching the solid medium supplemented with 2,4-D and exposed to light alone produced embryoids after one or two subcultures into MS medium with lower concentrations of 2,4-D. The embryoid production could be triggered if the calli were subcultured to liquid MS basal medium and when further transferred to solid media alone produced elongation of such embryoids. But the original explants had to be raised in MS medium supplemented with either TDZ or KIN as cytokinin for the embryoids to form subsequently. Encapsulated beads were successfully formed with nodal segments using 2.5 per cent sodium alginate and 75 mM calcium chloride with a complexation time of 30 min and the beads could be stored successfully for 15 days at room temperature and upto 40 days at 40 C. The peroxidase isozyme pattern of the leaves and roots from in vitro plantlets and in vivo plantlets were similar having the same number of bands
Open Access
Effect of planting density on the growth of rubber in the Vellanikkara estate
(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1993) Karthikeyan, K V; KAU; Nybe, E V
The present observations on the influence of different planting densities on the performance of two clones namely RRII 105 and RRII 118 are in conformity with the results of other field experiments reported earlier. In lower planting densities, trees produced good girth, high yield per tree, thick virgin bark and high rubber content. Whereas, high planting densities contributed to poor tree girth, high yield per tree, thick virgin bark and high rubber content. Whereas , high planting densities contributed to poor tree girth, thin virgin bark and lower yield per tree. However, planting density is dependent on various other factors viz. topography of the land, system of planting to be adopted, the planting materials, cost of labour and price of rubber. Anyway, a comprise between labour cost and rubber price is needed. As far as small holders are concerned, a higher stand per hectare is preferred, since the question of labour does not arise. Therefore, for small holding sector a higher stand is recommended in order to get high per hectare yield. But there should be an optimum stand. In Malaysia, for small holders having available family labour, a planting density of approximately 740 trees per hectare is recommended. However, indepth studies are required for a meaningful recommendation in our country. Salient results The trees were taller in high density planting The trees were more vigorous in low density planting. The highest mean girth of 64.7 cm was recorded by RRII 118 at a population density of 420 plants/ha.The percentage of weaklings increased with increase in population.The highest weakling percentage of 35.8 was recorded by the planting density of 824 plants per hectare. Poor bark growth in high density planting Per tree yield was more for low density planting whereas per hectare yield was less. With regard to high density planting , per tree yield was less and per hectare yield was more. Dry rubber content (drc) was more in low density planting. The highest percentage of 44.5 was recorded by RRII 118 at a planting density of 420 plants/ha. Light penetration was more in low density planting. The maximum light penetration recorded was 110.0 micromole/sec/m2 at a density of 420 plants per hectare.
Open Access
Enhancing the in vitro response of explants from mature jack (artocarpus heterophyllus lam.) trees
(Department of Horticulture, College of Agriculture, Vellayani, 1993) Reena, Philip; KAU; Rajmohan, K
The objective of the present study was to improve the propagation efficiency of mature phase jack trees by various pretreatments. The treatments tried involved stock plant treatments, explants treatments and in vitro treatments. Surface sterilization with HgCl2 for 13 minutes was found to be the most effective in reducing microbial contamination during culture establishment. Among the stock plant treatments tried, grafting on to juvenile rootstock was found to be the most effective in improving the in vitro response of explants from mature jack trees. Stress treatments were found to be the most effective among the explants treatments. Cold shock for 5 minutes at – 200C was found to produce the best results followed by heat treatment at 420 C for 2 minutes. Among the in vitro treatments, incorporation of phloroglucinol at the rate of 10mg/l was found to be the most effective. The results also showed significant influence of season on the response of mature explants, the highest response being observed during March – April.
Open Access
Feasibility of pineapple as an intercrop in small holdings of rubber in Muvattupuzha taluk
(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1993) Jose, V C; KAU; Tharian George, K
Muvattupuzha is a taluk where majority of people earn their living through agriculture. The literacy level of people is high. Among the crops raised, rubber is having great importance and it covers larger area. Almost all the rubber growers raise some intercrop during the first three years of immaturity of rubber to get some income. As an intercrop in rubber, pineapple has received much attention in recent years. The present study is an attempt to find out the benefits of growing pineapple as intercrop, to work out the B. C. R and to study its feasibility when compared to other cropping systems. Irrespective of the size of holding pineapple is used for intercropping in many units. Pineapple as intercrop was found to slightly retard the growth of rubber in the immaturity period and at tappable stage. Considering the long gestation period of 5 to 7 years and the profitability of intercropping during this period when there is no other income, it can be ignored. Cost analysis has proved that pineapple is a profitable intercrop in young rubber plantations. Among the varieties of pineapple, Mauritius is preferred due to higher profitability (B. C. R 2.4), easy management practices and marketability. Cultural operations for pineapple is not difficult, requiring minimum digging compared with other crops. Though the profitability depends on market price during harvesting period, it is fetching comparatively higher returns on account of comparatively stable prices. In Muvattupuzha taluk there is good marketing facility for pineapple. Fruits are sold in open market and through cooperative societies. The study revealed that soil erosion and weed growth are low or medium in pineapple intercropped plots. The study highlighted the potential of pineapple as an intercrop in rubber plantations in the taluk. The growing popularity of the crop is evident from the steady expansion of area under pineapple in all the villages of the taluk which was initially confined to selected regions.
Open Access
Induction of orthotrops in vegetatively propagated nutmeg (myristica fragrans houtt.) plants.
(Department of Plantation Crops and Spices, College of Horticulture, Vellanikkara, 1994) Rani, T G; KAU; Nazeem, P A
Investigations were made to induce orthotrops in vegetatively propagated nutmeg (Myristica fragrans Houtt.) plants at the Department of plantation Crops and Spices, college of Horticulture , vellanikkara, Thrissur during the period 1993-94. Budding orthotropic scions on there year old nutmeg plants alone showed bud take. In situ budding on three year old field plants gave the maximum bud take and bud sprouting . Among the different methods tried , maximum initial success was obtained with patch method in M. fragrans and forkert method in M. beddomei. The month of August was found to be the best season for in situ budding and July for budding in polybag plants. Partial shade was found ideal for better bud take than the mist . chamber conditions in nutmeg. Stumping the root stock above the bud union retaining the whorl of lower leaves gave better sprouting and later survival. The anatomical studies of bud union revealed the sequence of bud union as the callus formation stages , callus bridge stage and cambial differentiation stage. Absence of callusing , damage to cambial layers of stock or scion, thick necrotic layer and a wide gap between stock and scion and phenolic exudation were attributed to the probable reasons for bud failure . On mature branches of nutmeg trees, maximum number of orthotrops were induced when an orthotrop was cut close to the tree trunk leaving 30 cm stump. Staking the immature brown shoots of one to two years age and young green shoots of less than six months of nutmeg grafts acquired a reduced angle with the vertical even one month after removal of stake. Among the physical and chemical treatments tried to induce orthotrops in nutmeg grafts of plagiotropic nature, the treatments stumping and application of 5 ppm kinetin was found superior with respect to the number of shoots produced per plant. However , none of the shoots were found to have orthotropic growth pattern. Anatomy of orthotropic and staked stems revealed the production of more wood towards the adaxial side than towards the adaxial side. Plagiotropic stem have more or less proportionate wood formation on both sides.
Open Access
Standardisation of propagation techniques in schefflera
(Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 1997) Sunitha Anne, Mathew; KAU; Murali, T P
The study was carried out at the Department of Pomology and Floriculture, Vellanikkara and Agricultural Research Station, Mannuthy from March 1994 to February 1996 to standardise the propagation techniques in schefflera arboricola). Schefflera, a member of the botanical family, Araliaceae is valued for its ornamental foliage. Not much information is available in the literature on agro techniques for the commercialization of this important foliage plant. Hence, the present study “Standardisation of propagation techniques in schefflera” has great relevance. In both ‘green’ and ‘variegated’ type of schefflera, double noded cuttings performed better than single noded cuttings. The number and quality of roots produced were improved with growth regulator treatments and the prolonged dip method was found to be the best in both ‘green’ and ‘variegated’ type of schefflera. The best growth regulator and its optimum concentration for rooting of cuttings in ‘variegated’ type was IBA at 200 mg 1-1 whereas in ‘green’ type, NAA at 50 mg 1-1 was found to be an effective treatment. Percentage success in rooting of cuttings depended on the growth regulator employed. In ‘variegated’ type of schefflera the percentage success obtained in rooting of double noded and single noded cuttings could be improved with IBA treatment and in ‘green’ type, NAA treatment was found to be beneficial. In layering also growth regulator treatment was found to be beneficial. In ‘variegated’ type NAA at 50 mg 1-1 produced maximum rooting whereas in ‘green’ type NAA at 200 mg 1-1 produced longer and stouter roots. The media used and the method of wounding adopted in layering were found to have significant influence on rooting behaviour. Girdling was found to be more effective compared to slanting slit method. The best media were sphagnum moss and sawdust in ‘variegated’ type whereas in ‘green’ type, sawdust was the best medium. Percentage success in rooting of layers depended on the growth regulator, media and type of wounding method employed. The percentage success obtained in rooting of layers (‘variegated’ and ‘green’) could be improved with an NAA treatment, using sawdust as the medium and girdling as the wounding method. A comparison of the methods of propagation revealed that in schefflera cuttings could be adopted as reliable and successful propagation method to produce large number of plants in a short time from limited amount of planting materials. In micropropagation, callus was formed from immature and young leaves and the callus production was good with 2, 4-D at 1-2 mg 1-1 and NAA at 10-12 mg 1-1 but the calli did not respond to caulogenesis. In direct organogenesis, axillary bud break from nodal explants was noticed in MS medium with BAP at 0.5 mg 1-1 and the shoot growth was the best with BAP at 5 mg 1-1. The in vitro developed shoots were rooted in the medium supplemented with NAA at 3 mg 1-1 IBA at 0.3 mg 1-1. Further studies are needed to standardise a complete protocol for micropropagation of S. arboricola.
Open Access
Environmental effects on the growth of philodendron wendlandii
(Department of Pomology & Floriculture, College of Horticulture, Vellanikkara, 1996) Swapna, S; KAU; Geetha, C K
An experiment was carried out at the Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, to evaluate the environmental influence on the growth of Philodendron 'Wendlandii'. The effects of media, containers and fertilizer forms and doses were assessed at three levels of shade, namely, 25, 50 and 75 per cent. Results revealed that treatments could significantly influence all the vegetative parameters, viz., plant height, number of leaves, total leaf area and number of side shoots, at different stages of growth. The superiority of the combination of peat, mud pot and soluble fertilizer at its higher concentration was clearly evident with respect to plant height at 25 and 50 per cent shade levels. Controlled release fertilizer substituted in the above combination recorded plant height on par with this, at 25 and 50 per cent shade levels whereas the height was significantly superior at 75 per cent shade level. Number of leaves was higher in the case of peat + mud pot + controlled release fertilizer under all the three shade levels. The above treatment combination produced more leaf area under 50 per cent shade. This was comparable with that of the leaf area produced when soluble fertilizer was used at 75 per cent shade. Number of side shoots was also higher in a combination of peat + mud pot + controlled release fertilizer. Total biomass was a good indicator of the superiority of peat + mud pot -\- controlled release fertilizer at 25 and 50 per cent shade levels. The response in uptake was more in the case of peat supplied with controlled release fertilizer. Better plant quality was observed when grown in peat and mud pot. The shade level of 50 per cent was considerably better with respect to all the growth parameters, such as, height, number of leaves and total leaf area. Although chlorophyll content was maximum under 75 per cent shade, it was on par with that at 50 per cent shade. Overall plant quality too showed superiority of 50 per cent shade level.

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