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  • ThesisItemOpen Access
    Evaluation of selected underutilized flowers of Kerala for commercial exploitation
    (Department of Pomology and Floriculture, College of Horticulture, Vellanikkara, 2018) Sameera Sharief; KAU; Sudhadevi, P K
    Floriculture industry is unique among agricultural industries where novelty is an important attribute. World floriculture is expanding rapidly and new innovations and introductions are in great demand to feed the ever hungry market needs. It is in this interest that neglected or underutilized flower crop species (NUS) comes to the picture from which we could identify and develop diversified uses of floriculture. Even the present day top charactered crops of the industry are nothing but just derived and developed only from wild germplasm resourses, the most prominent among them being rose, chrysanthemum, carnation, gerbera and what not, the orchids and anthurium. Thirteen underutilized plants of Kerala were evaluated for use as cut flowers, dry flower, for essential oil extraction and pigment extraction and identification of components in their essential oils and pigments using GC-MS by conducting both field studies as well as postharvest studies. Out of the 10 plants selected for studying their use as cut flower, none were found suitable. Five plants were selected for their suitability for dry flower production. Among them, Cassia fistula got the highest cumulative score followed byAntigonon leptopus,Calicopteris floribunda and Barleria obtusa. Least cumulative score was obtained for Clerodendrum paniculatum. In Antigonon leptopus and Clerodendrum paniculatum best method of drying was microwave oven drying. Press drying was selected as the best method for Barleria obtusa and Calicopteris floribunda. Embedded drying was found the most suitable method of drying in Cassia fistula. The fragrant flowers selected for extraction of essential oils were Gardenia jasminoides, Plumeria spp and Quisqualis indica.Maximum essential oil yield was observed in Gardenia jasminoides (0.61%). The components in the essential oils were identified by gas chromatography and mass spectrometry. In Gardenia jasminoides the components identified were Ascalbin (0.7%), Nonadecane (0.80%), Dendaralasine (0.96%), Alpha famesene (1.12%), Linalool (1.38%), Beta famesene (2.75%), Famesene (3.04%), Henecosane (5.26 %), n-Tricosane (6.91%), n-octacosane (10.43%), Pentacosane (13.19%), Monoethylhexyl phthalate (44.74 %). The volatile components identified in Plumeria were α-Farnesene (1.08%), Benzoic acid, [(E,E)-3,7,11-trimethyl- 2,6,10-dodecatrien-1-yl] ester (1.08%), 1,6,10-Dodecatriene, 7, 11- dimethyl- 3- methylene-E(1.17%), Cyclohexasiloxane, dodeca methyl (1.92%). Phenyl ethyl alcohol (2.20%), 1,3,6,10-Dodecatetraene, 3,7,11-trimethyl-(Z,E)(2.93%), Oxalic acid, decyl 2-phenyl ethyl ester (4.3%), Triphenyl phosphare (4.3%), Heptacosane (6.32%), Nonacosane(6.40%) and Z-14-Nonacosane (6.40%) and Z-14- Nonacosane(11.65%). The components responsible for fragrance in Quisqualis indica were 2 H- Pyran-3-ol, 6-ethenyl tetra hydro-2,2,6-trimethyl(1.24%), Heptacosane (1.44%), Cyclotetra siloxane, octamethyl (1.95), Triphenyl phosphate (2.30%), Nonacosane(2.89%) and 5-Isoquinoline carbonitr (12.5%). The flowers selected for extraction of pigments were Caesalpinia pulcherrima, Cassia fistula, Clerodendrum paniculatum and Delonix regia. Two methods of extraction selected were solvent extraction after fermentation and solvent extraction after pretreatment with NaOH. In the entire species pigment yield was higher for solvent extraction after pretreatment. Highest oleoresin yield was observed in Clerodendrum paniculatum (0.60g), which was followed by Delonix regia (0.5g) and Cassia fistula(0.39). The lowest yield was observed in Caesalpinia pulcherrima (0.38g). After fermentation, Clerodendrum paniculatum gave highest oleoresin yield (0.43g). This was followed by Delonix regia(0.4g), Cassia fistula(0.3g) and Caesalpinia pulcherrima(0.28g). Highest carotenoid yield was observed in Cassia fistula (70.04mg/ 100 g) and highest anthocyanin yield was in Clerodendrum paniculatum (574.76mg/100g)). In Caesalpinia pulcherima carotenoid content was recorded as 15.35mg/100g and anthocyanin 488.75mg/100g. Anthocyanin yield of Cassia fistula was 0.35mg. Clerodendrum paniculatum recorded 2.98mg of carotenoid. Delonix regia recorded carotenoid and anthocyanin yield of 60.2 mg and 510 mg respectively. In the present study none of flowers were found suitable for use as cut flower. Out of the 5 plants selected for studying for use as dry flower, Cassia fistulawas the most suitable one. All the species selected for essential oil extraction were suitable for the purpose .In pigment extraction, highest oleoresin yield was observed in Clerodendrum paniculatum (0.60g), which is followed by Delonix regia (0.5g) and Cassia fistula(0.39). Future line of work suggested in this aspect based on the light of results are evaluation of more underutilized ornamental flowers available in our locality with a view of their commercialisation for specific traits and further evaluation of extracted pigments for their use in food industry.
  • ThesisItemOpen Access
    Nutrient management in gerbera (Gerbera jamesonii Bolus.) grown under naturally ventilated polyhouse
    (Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2018) Anaswara, S J; KAU; Sheela, V L
    The thesis work entitled “Nutrient management in gerbera (Gerbera jamesonii Bolus.) grown under naturally ventilated poly house” was carried out at the Department of Pomology and Floriculture, College of Agriculture, Vellayani during 2016-2018. The study was to assess the performance of five gerbera varieties to different doses of fertilizers with a view to find out the best fertilizer dose for each variety. This study also aimed for the standardization of nutrient management schedule for Gerbera jamesoniiBolus. Five varieties were selected for the study (V1- Beaudine, V2- Double date, V3- Mammut, V4- Esmara, V5- Aquamelon). Different nutrient treatments (N1- 10:10:10 N, P2O5, K2O g m-2, N2- 15:15:15 N, P2O5, K2O g m-2, N3- 20:20:20 N, P2O5, K2O g m-2) were applied to the varieties at monthly and fortnightly interval. The experiment was laid out in completely randomised design consisting of thirty treatment combinations and three replications. The study revealed that variety Beaudine was significantly superior in terms of vegetative parameters viz., plant spread (156.19cm) at 4 MAP, number of leaves (13.31) at 8 MAP and leaf length (37.09cm) at 4MAP. Variety Mammut was significantly superior in leaf breadth (11.03cm) at 10 MAP and number of suckers plantˉ1(2.59) at 6 MAP. The nutrient treatment 10:10:10 N, P2O5, K2O g mˉ2 produced significantly higher plant spread (171.29cm) at 10 MAP, number of leaves (12.54) at 6 MAP, leaf length (34.04cm) at 4 MAP, leaf breadth (12.35cm) at 4 MAP and number of suckers plant-1 (2.97) at 6 MAP. The level of split application of nutrients s1 showed significantly superior values for plant spread (148.86cm) at 4 MAP, number of leaves (10.95) at 8MAP, leaf length (31.67cm) at 4 MAP, leaf breadth (10.79cm) at 4MAP and number of suckers plantˉ1 (2.42) at 6MAP. The variety Esmara when treated with 10:10:10 N, P2O5, K2O g mˉ2 at monthly interval of split application of nutrients recorded significantly highest value for plant spread (183.38cm) at 10 MAP. Variety Beaudine treated with 10:10:10 N, P2O5, K2O g mˉ2 (1.6: 1.6: 1.6 N, P2O5, K2O g plantˉ1)at monthly interval of split application of nutrients recorded significantly higher values for number of leaves (15.23) at 6MAP and leaf length (46cm) at 4 MAP. Variety Mammut treated with 10:10:10 N, P2O5, K2O g mˉ2 split application of nutrients at monthly intervals recorded significantly higher values for leaf breadth (15.74cm) at 4 MAP and number of suckers plantˉ1 (3.89) at 6 MAP. Regarding the flowering characters, Variety Esmara treated with 10:10:10 N, P2O5, K2O g mˉ2 of split application of nutrients at monthly intervals recorded the lowest value for number of days taken for flowering (17) and variety Double date treated with 15:15:15 N, P2O5, K2O g mˉ2 at fortnightly interval of split application of nutrients recorded lowest value for number of days taken from bud initiation to harvest (7.9) and there was no significant difference on flower longevity on the plant. In flower characters, Mammut variety supplied with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded highest flower diameter (11.24cm) and width of ray florets (1.00cm).There was no significant difference observed for flower disc diameter and length of the ray florets. Esmara variety with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded the highest number of ray florets (65.01).Beaudine variety with a combination of 10:10:10 N, P2O5, K2O g mˉ2 fertilizers at monthly interval of split application of nutrients recorded the highest stalk girth (2.39cm) and stalk length (79.34cm). In yield parameters, highest number of flowers produced plant-1year-1(25.75) and higher yield of flowers in relation to season (15.25) at rainy season was recorded for variety Esmara treated with a combination of 10:10:10 N, P2O5, and K2O g mˉ2 with a split application of nutrients at monthly intervals. The vase life of flowers and water uptake showed no significant difference among the treatment combinations All the five varieties while treated with 10:10:10 N, P2O5, K2O g mˉ2 at monthly intervals resulted high values for vegetative, flower and flowering characters. The best performance for Beaudine followed by Esmara and Aquamelon. BC ratio was highest for Beaudine. Future line of work may be standardisation of agrotechniques for gerbera under Kerala conditions.
  • ThesisItemOpen Access
    Organic nutrient management of papaya (Carica papaya L.)
    (Department of Pomology and Floriculture, College of Agriculture, Vellayani, 2018) Divya Hari; KAU; Bindu, B
    The present study entitled “Organic nutrient management in papaya (Carica papaya L.)” was carried out in the Department of Pomology and Floriculture, College of Agriculture, Vellayani during 2016-2018, with an objective to study the effect of organic nutrient management on growth, yield and quality of papaya. The experiment was conducted in RBD with 11 treatments and 3 replications using papaya variety Surya. The treatments included in the study are: T1-100% of recommended dose of N as organic, T2-75% of recommended dose of N as organic, T3-100% of recommended dose of N as organic + PGPR Mix-I, T4-75 % of recommended dose of N as organic + PGPR Mix-I, T5-100% of recommended dose of N as organic + AMF , T6-75% of recommended dose of N as organic + AMF, T7-100% of recommended dose of N as organic + PGPR Mix-I+ AMF , T8-75% of recommended dose of N as organic + PGPR Mix-I+ AMF, T9-Full NPK of KAU POP + Vermicompost (N equivalent to FYM of KAU POP), T10- 100% RDF (240:240:480 g NPK plant-1 year-1, FYM-10 kg plant-1 year-1 ) , T11- Absolute control. The present study revealed that, application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF increased the girth of the plant, number of leaves, fruit set percentage with lowest height for first flowering and reduced the number of days for flowering. The highest plant height was obtained by the application of 75% of recommended dose of N as organic along with AMF. With regard to the yield characters studied, highest fruit weight, fruit length, fruit girth, fruit volume, pulp percentage, flesh thickness, total yield plant-1 (39.91 kg plant-1) and minimum number of days for maturity were recorded by the application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF. Number of fruits plant-1 was found to be highest with application of 100% RDF (240:240:480 g NPK plant-1 year-1, FYM-10 kg plant-1 year-1). The study on quality parameters of fruit revealed that TSS, ascorbic acid, carotenoids, total sugar, reducing sugar and the organoleptic qualities were highest with the application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF. Analysis of soil nutrient status revealed that soil organic carbon, soil EC, nitrogen and potassium content were highest with the application of 75% recommended dose of N as organic along with AMF. Phosphorus, micronutrient content and microbial count were highest with the application of 100% recommended dose of N as organic along with AMF. The leaf petiole analysis of papaya indicated that the highest phosphorus, potassium and micronutrient content increased with the application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF. Regarding the B: C ratio and net income obtained, application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF recorded the highest. Overall assessment indicated that application of 100% of recommended dose of N as organic along with PGPR Mix-I and AMF in 6 splits at bimonthly interval improved the growth, yield, quality of papaya.
  • ThesisItemOpen Access
    Standardization of grafting techniques in african marigold (tagetes erecta L.) for combating bacterial wilt
    (Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara, 2018) Athira Baburaj; KAU; Sreelatha, U
    The present study entitled “Standardization of grafting techniques in African marigold (Tagetes erecta L.) for combating bacterial wilt” was taken up in the Department of Floriculture and Landscaping, College of Horticulture, during the year 2017-2018. The work was carried out under three experiments viz.,standardisation of grafting techniques, assay of enzyme activity and histological studies. The rootstock used in the study was the bacterial wilt resistant local collection M- and the wilt susceptible F1hybrid Maria 91 was used as scion. Grafting was done in the month of January. Three methods of grafting viz., cleft, splice and hole insertion were tried at different ages of rootstock (4, 5, 6 and 7 weeks after sowing) and different ages of scion (3, 4 and 5 weeks after sowing). Observation of graft survival was taken at 15 DAG (Days after grafting). It was observed that cleft grafting four week old scion on to six week old rootstock was found to be the best with a graft survival of 61 percent. It was also observed that there was no survival when grafting was done on four week old rootstock irrespective of age of scion and grafting method followed. Estimation of catalase and peroxidase was carried out to study the activity of these enzymes in graft union formation. Sampling was done at 4,8 and 15DAG. It was observed that, activities of both the enzymes increased from 4DAG to 15DAG. Highest catalase activity at 4th (0.85 EU/g), 8th (1.39 EU/g) and 15th (1.60 EU/g) DAG was observed in cleft grafting of four week old scion on to six week old rootstock. Similar results were also observed in peroxidase activity. Correlation of catalase and peroxidase activity with survival of grafts indicated that both the enzymes are positively correlated with graft survival. Histological studies of the grafts consisted of both anatomical studies and histochemical staining for lignin. It was observed that there was an intensification of the stain from 4DAG to 15DAG with advancement of lignification process in graft union. Histological studies also revealed the formation of necrotic layer in the graft union, as a result of wound repair and cell proliferation. Cleft grafting of four week old scion on six week old wilt resistant rootstock is the best method in African marigold for combating bacterial wilt.
  • ThesisItemOpen Access
    Evaluation of rose varieties for commercial cultivation under the warm humid tropics of Kerala
    (Department of Floriculture and Landscaping, College of Horticulture, Vellanikkara, 2018) Priya Philip; Mini Sankar
    An investigation entitled "Evaluation of rose varieties for commercial cultivation under the warm humid tropics of Kerala "was conducted in a naturally ventilated polyhouse of Department of Floriculture and Landscaping during August 2017 to March 2018. The objective of the study was evaluate the performance of cut flower and loose flower rose varieties under polyhouse and to select suitable varieties of both types for commercial cultivation in the plains of Kerala. Ten cut flower varieties viz. Arka Ivory, Arka Pride, Arka Swadesh, Gold Strike, Noblesse, Revival, Taj Mahal, Corvette, Emma, Peach Avalanche and ten loose flower varieties viz. Arka Parimala, Sherba Gold, Mirabel, Vernish, Charisma, Jadiov, Star Light, Spray Orange, Spray Yellow and Red Varnish were evaluated for vegetative as well as floral and genetic parameters. The variety Taj Mahal was superior in plant height (52.83 cm) at peak flowering stage. Highest number of sprouts (2.83) was observed in variety Gold Strike whereas varieties Revival (5.5) and Peach Avalanche (5) had highest number of branches. Variety Arka Ivory (38.67) was found to have highest number of leaves at peak flowering stage. Stalk length, bud size, number of petals per flower and keeping quality are the important quality criteria for cut flowers in National and International markets. The highest stalk length was observed in variety Emma (30.48 cm) and Arka Pride (31.35 cm) whereas variety Taj Mahal (79.1) was superior in terms of number of petals. Length and diameter of flower bud was superior in varieties Arka Ivory (2.77 cm and 1.09 cm) and Taj Mahal (2.53 cm and 1.21 cm). Longest vase life was observed in varieties Revival (4.83 days), Noblesse (4.75 days) and Taj Mahal (4.75 days). Highest number of marketable flowers was observed in varieties Arka Ivory (8.45), Taj Mahal (8.28) and Revival (7.23). Among the loose flower varieties the variety Arka Parimala was superior with respect to plant height (59.82 cm) and number of branches (6.67). Variety Charisma had highest number of sprouts (4) whereas variety Star Light was superior with respect to number of leaves per branch (44.47). Varieties varied significantly with respect to floral characters. Highest number of petals was observed in variety Star Light (101) and variety Arka Parimala was found to have longest flower bud (2.6 cm), highest flower diameter (6.7 cm) and strong fragrance whereas this variety was inferior with respect to number of petals (15.4). Yield of marketable flowers was highest for the varieties Arka Parimala (34.33 g), Mirabel (20.83 g), Vernish (28.39 g), Charisma (28.7 g), Spray Orange (26.93 g) and Red Varnish (29.28 g). Variety Star Light was found to be superior in terms of overall performance. The marketable flower yield over the months of observation indicated that November – December is the best season for rose production after the October pruning. High temperature and low humidity along with severe incidence of thrips reduced marketable yield during Feruary – March. Dendrogram based on D2 statistics indicated that there exists much diversity among the cut flower and loose flower varieties for both quantitative and qualitative characters. The genetic estimates, GCV and PCV were found to be high for the characters viz. number of flowers per sprout (24.11 , 32.30), number of petals per flower (40.08, 43.56), yield of marketable flowers (27.71, 48.86) and fresh weight of the flower (21.19, 23.61) indicating wide variability for these characters among the ten cut flower varieties. The characters number of petals per flower (84.63,75.95), fresh weight of the flower (80.56, 39.18) and vase life (69.53, 26.96) exhibited high heritability coupled with high genetic advance indicating additive gene action in the expression of these characters and are highly inheritable while selection done based on these characters for cut flowers. Among loose flowers, the genetic estimates GCV and PCV were found to be the highest for the characters number of flowers per sprout (42.25, 52.45), number of flowers per plant (26.93,34.43), number of petals per flower (55.42,55.58), fresh weight of flower (61.69, 93.84), weight of petals per flower (83.81, 120.33) and flower diameter at fully opened stage (25.89, 28.71). High heritability coupled with high genetic advance were observed for the characters number of flowers per sprout (64.89,70.11), number of flowers per plant (61.19, 43.40), number of petals per flower (99.43, 50.12) and flower diameter at fully opened stage (81.32,48.10) indicating the existence of additive gene action and selection based on this characters will be effective. Correlation and path analysis revealed that the characters viz. length of flower bud (1.041), number of flowers per plant (0.156) and number of petals per flower (1.045) were significantly and positively correlated with marketable yield per plant along with direct effect (1.131, 0.251, 0.285 respectively). Since, the characters number of flowers per plant and number of petals per flower had high GCV (%), PCV (%), high heritability (%) coupled with genetic gain (%) along with positive significant correlation and direct positive effect on marketable yield, these characters can be fixed as the selection criteria for loose flowers.