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ThesisItem Open Access Utilization of prawn waste as pig feed(Department of Animal Nutrition, College of Veterinary and Animal Sciences, Mannuthy, 1991) Syam, Mohan K M; KAU; Sivaraman, EAn investigation was carried out to assess the feeding value of prawn waste as a partial or complete replacement of unsalted dried fish in the rations for growing – finishing pigs. Thirty – two Large White Yorkshire weanling pigs with an average body weight of 9.1 kg were distributed randomly and uniformly as far as possible to four groups (Groups 1, 11, 111 and IV) of eight animals each, with regard to age, sex and body weight and housed in pairs of the same sex. The four dietary treatments A, B, C and D were allotted to the pigs in the groups 1, 11, 111 and IV respectively. Of the total protein, 25 per cent in the diets A, B and C and 12.5 per cent in the diet D were provided as animal protein. Unsalted dried fish was used as animal protein source in the diets C and D. Diet C formed the control diet. In the diets A and B, 50 per cent and 100 per cent replacements respectively of animal protein from unsalted dried fish were made using dried prawn waste. All the animals were maintained on the respective diets with 18 per cent total protein upto an average live weight of 50 kg and with 14 per cent protein till they attained a body weight of 70 kg or 8 months of age, whichever was earlier, when they were slaughtered to study carcass characteristics.ThesisItem Open Access Development of Malaysian Type Fish Crackers From Nemipterus Japonicus (BLOCH)(Department of Processing Technology,College of Fisheries, Panangad, 2001) Venugopal, Dubakula; KAU; Sajan, GeorgeA study was undertaken for the preparation of fish crackers using a low cost fish, threadfm bream (Nemipterus japonicus, Bloch). A method was standardised for making crackers based on that generally followed in Malaysia. Fish meat was mixed with various food additives such as starch, salt, sugar, monosodium glutamate, colour and water, and stuffed into high-density polyethylene casings as rolls, cooked, cooled, sliced and dried.ThesisItem Open Access Studies on the electrophoretic pattern of Fish/ Shellfish proteins subjected to frozen storage(Department of Processing Technology,College of Fisheries, Panangad, 2000) Bomy, Chummar; KAU; Nambudiri, D DFreezing considered as an excellent process for preserving the quality of fish for longer periods. But freezing may affect various flesh components, especially the proteins. Sarcoplasmic proteins play a major role in species identification of fish and fishery products. While myofibrillar proteins play a major role in the jellying properties of surimi and surimi based products. The changes to these groups of proteins due to freezing and frozen storage have received much attention. Four species Megalaspis cordyla, Labeo rohita, Penaeus indicus and Parapenaeopsis styli/era were used for the frozen storage study. Samples were stored for 90 days at -18°C and samples were collected at 15 day. intervals and Salt Soluble Nitrogen (SSN), Water Soluble Nitrogen (WSN) and Non-Protein Nitrogen (NPN) content were determined. Electrophoretic pattern of both Salt Soluble Proteins (SSP) and Water Soluble Proteins (WSP) of frozen stored sample were compared with that of the fresh sample.ThesisItem Open Access High density rearing of Labeo Rohita (Hamilton) spown indoors using different feeds(Department of Aquaculture, College of Fisheries, Panangad, 1998) Dinesh, K; KAU; Mohankumaran, Nair CThree day old rohu spawn was reared in indoor tanks with eight different feeds The diets tried were newly hatched Artemia nauplii, size graded Moina micrura, formulated feed, Artemia nauplii + formulated feed, Moina micura+ formulated feed, first 3 days Artemia nauplii and formulated feed later on, first 3 days Moina micrura and formulated feed later on and mixed zooplankton + ricebran and GOC in I: I ratio (conventional method).ThesisItem Open Access Impact of training programme on hygienic measures followed in pelling sheds(Department of Management Studies, College of Fisheries, Panangad, 1989) Daisy, C Kappen; KAU; Pushkaran, P SThe study was conducted in Quilon district of Kerala with a view to measure the impact of training programme on hygienic measures followed in peeling sheds. The major objectives were: 1. To study the level of knowledge about hygienic measuroe of trained peeling shed workers in comparison with those who have not attended the training programme. 2. To study the perception of the trained peeling shed workers about the quality of the product. • 3. To compare the association, if any, existing between the socio-psychological and economic factors of trained and untrained peeling shed workers with the level of knowledge and extent of perception. 4. To study the motivational factors responsible for attending the training programme by the peeling shed workers. The sample consisted of randomly selected 60 trained and 60 untrained peeling shed workers. Data were collected using interview schedule and suitable statistical techniques were employed for the analysis of data. The study revealed that the trained peeling shed workers had significantly higher knowledge than untrained peeling shed workers. They also had significantly higher perception about the quality of the product than untrained peeling shed workers. The peeling shed workers perceived the factor ‘Quality of the raw material’ as the most important one in determining the final quality of the product. The perception on the factors contributing to the final quality of the product were same for both trained and untrained peeling shed workers. The selected independent variables together contributed significantly in the variation in knowledge and perception about the quality of the product of both trained and untrained peeling shed workers. The correlation analysis revealed that education was positively and significantly related with level of knowledge of trained peeling shed workers; while age, occupation and family indicated negative and significant association. In the case of untrained peeling shed workers extension orienta¬tion is the only variable Which Indicated positive and signi¬ficant relationship with level of knowledge. Education showed positive and significant relationship with perception about the quality of the product of trained peeling shed workers. Age and occupation revealed negative and significant association. In the case of untrained peeling shed workers only age Indicated negative and significant relationship with perception about the quality of the product. Innovativeness was the major motive which induced the peeling shed workers to attend the training programme.ThesisItem Open Access Studies on depuration of edible oyster Crassostrea madrasensis (Preston)(Department of processing technology, College of fisheries, Panangad, 1993) Usha, P T; KAU; Damodaran, NamboodiriOysters crassostrea madrasensis harvested from cochin back waters are commonly contaminated with low levels of food poisoning organisms such as E.coli, Salmonella, Shigella, Vibrio cholera, V-parahaemolyticus etc, heavy metals like cadmium, lead, copper, zinc, mercury etc and sand content. Depuration studies were conducted to find out any changes in the biochemical constituents such as total Nitrogen, salt soluble Nitrogen, Non protein Nitrogen, Ash and sand content. The studies revealed that there were no significant changes in the biochemical constituents during the 48th depuration period. Oysters were laboratory contaminated to levels in excess of 10000 cells /g with E.coli and it was cleansed from such oysters during purification in a laboratory depuration unit that used ultraviolet light and chlorination for sterilizing the depuration water. Depuration in sterilized water using ultra violet light treatment was found to be more efficient in cleansing the oyster of pathogen, E.Coli than using chlorination. Of the two salinity tested depuration of oyster in seawater at 35 ppt salinity gave better results compared t that in 30ppt salinity sea water both sterilised with uv light. Depuration of oyster in unsterile seawater and in seawater sterilised 10 ppm chlorination was found to be least effective in cleansing the oyster to the acceptable international standard of less than 2.3 E.Coli/g oyster meat within 48h depuration. There was no appreciated change in the sensory characteristics of oysters such as aroma, taste, and flavor. However there was significant change in the grittiness characteristics of oyster after 48h depuration. Depuration was not effective in removing heavy metals such as cadmium, lead, zinc, Tin and Mercury from the oyster within the depuration period of 48h.ThesisItem Open Access Breeding biology of Villorita cyprinoides (Gray) in relation to salinity gradients(Department of Fishery Biology, College of Fisheries, Panangad, 1991) Sudha, B Nair; KAU; Jose, T MThe present study was undertaken to investigate the influence of salinity variations on the breeding biology of populations of black clam Villorita cyprinoides (Gray), inhibiting two different cological zones of the Vembanad Lake. Two stations with perennial clam beds, but differing greatly in salinity conditions were selected for the study. Station I, in the northern side of Thanneermukkam barrier, has more influx of sea water and Station II, in the southern side has low saline influx. Monthly collections of black clams and bottom and surface waters were made from May 1989 to June 1990. Major environmental parameters such as salinity, temperature, dissolved oxygen and pH of bottom and surface waters were estimated with a view to understand the circannual variations within and between the stations. Among these, salinity was found to be the most important parameter showing prominent variations. At Station I, the bottom salinity values ranged from 0.0 to 18.12%0 and in Station II from 0.0 to 2.12%0. Histological studies of the clams collected from the two stations revealed that animals inhabiting Station I, a predominantly brackish water zone, have a protracted, almost year round breeding season extending from September to June with peak spawning during March to June and coinciding with the peak salinity levels and temperature. At Station II, a predominantly freshwater zone, the clams have a short duration spawning season extending from March to June with peak spawning during Apirl and May, again coinciding with the peak salinity levels and temperature. It is also observed that there exists a positive correlation between the bottom salinity and gondal maturation and spawning. Size – frequency studies of the calms collected from the two stations revealed that at Station I, the clam fishery was dominated by small sized animals, when compared to Station II. This may be related to the differences observed in the extend of spawning activity between the population of the two stations, resulting in the divergent apportionment of energy resource for somatic growth versus reproduction.ThesisItem Open Access Investigations on head drooping In head-on processed prawns(Department of Processing Technology, College of Fisheries, Panangad, 1994) Jose, V T; KAU; Sherief, P MHead drooping is the major quality problem associated with processing of headon prawns. The head drooping in head on prawns refers to the condition of oozing out of hepatopancreatic juice resulting from the breakage of the arthrodial membrane, which connects cephalothoraz of prawn to abdomen dorsally. The susceptibility of prawns to head drooping depends on the stability of the arthrodial membrane which in turn is determined by its biochemical composition. In this connection various biochemical constituents of the arthrodial membrane such as protein, phospholipid, moisture, calcium and cholesterol were analysed for two specied of prawn, Penaeus indicus and Penaeus monodon under three size groups (30/40, 50/60, and 70/80 pieces /kg). The activities of various hydrolytic enzymes of the hepatopancreas were also studied. The various biochemical constituets showed significant difference between species. Within the same species a size wise variation in the constituents of the arthrodial membrane and enzyme activity of hepatopancreas was observed. The various unit process such as hauling time, ice storage durations, freezing methods, cold storage durations and thawing methods were studied individually for their influence on head drooping. The study shows that the major determinants of head drooping in prawns are the biochemical composition of the arthrodial membrane and the easiness with which the hydrolytic enzymes of the hepatopancreas are released on postmortem handling and processing.ThesisItem Open Access Quantification of ammonia excretion by selected five varieties of freshwater ornamental fishes(Department of Aquaculture, College of Fisheries, Panangad., 2006) Senthamilselvi, A; KAU; Mohan, M VAmmonia excretion of five species of freshwater ornamental fishes, platy (Xiphophorus maculatus), swordtail (Xiphophorus helleri), angelfish (Pterophyllum scalare), goldfish (Carassius auratus) and blue gourami (Trichogaster trichopterus). Fishes acclimated for two weeks were incubated for a specific period in closed containers and the ammonia excretion rates were determined. Ammonia was determined using phenol hypochlorite method. Regression analysis of ammonia excretion rate and weight showed that ammonia excretion rate increased with body weight. The regression coefficient of the relationship was 0.4320 in platy, 0.7534 in swordtail, 0.4851 in angelfish, 0.6309 in goldfish and 0.6518 in blue gourami. The weight specific ammonia excretion rate decreased with bodyweight and the slope of the weight specific ammonia excretion rate – weight relationship was - 0.5680 in platy, - 0.2466 in swordtail, - 0.5149 in angelfish, - 0.3691 in goldfish and - 0.3482 in blue gourami. The regression coefficient of total length- weight and standard length- weight relationship are 3.1031 and 3.0565 for platy, 3.1512 and 3.1454 in swordtail, 3.0331 and 2.9656 in angelfish, 2.6191 and 3.0328 in goldfish and 2.9721 and 3.0011 for blue gourami, respectively. The regression coefficients were significantly different from 3 in both the relationships. The weight specific ammonia excretion rate ranged from 0.0032 to 0.0640 g NH3-N/mg/h for platy, 0.0028 to 0.0140 g NH3-N/mg/h for swordtail, 0.0033 to 0.1309 g NH3-N/mg/h for angelfish, 0.0043 to 0.0525 g NH3-N/mg/h for goldfish and 0.0091 to 0.0394 g NH3-N/mg/h for blue gourami. Ammonia excretion rate and weight specific ammonia excretion rate were highest for blue gourami, followed by goldfish, angelfish, swordtail and platy but the weight specific ammonia excretion rate was highest in platy than swordtail for the smaller size group. Quantification of ammonia excretion for standard weight and length facilitates prediction of ammonia build up in a freshwater aquarium