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  • ThesisItemOpen Access
    Effect of sedatives on penaeus monodon fabricus seed under oxygen packing for transportation
    (Department of Aquaculture, College of Fisheries, Panangad, 1991) Joshi, K; KAU; Jayasree Vadhyar, K
    The study was performed with the objective of selecting a sedative at a suitable dose and testing its effect along with other factors viz. packing density, salinity and temperature on the P.mondon seed under oxygen – packed transport conditions. The selection of a sedative at an appropriate dose, from those tried viz. chloral hydrate, MS-222 and tertiary butyl alcohol, was made by conducting statistically designed experiments on the survival of the treated and untreated prawn seed for 72 hours in open containers and on the metabolic activities of the treated and untreated prawn seed for 2 hours. The experiment to find out the effect of the selected sedative was carried out in specially designed hard plastic containers fitted with facilities fpr packing oxygen under uniform pressure. The experiment was conducted as an asymmetrical factorial experiment with 4 levels of packing density (200/1, 400/1, 600/1 and 800/1) and 2 levels of sedation (without and with sedation), salinity (25 ppt and 20ppt) and temperature (29+ 10C and 23 + 20C). Chloral hydrate was selected at a dose of 400 ppm for application on P.monodon post –larvae under oxygen – packed conditions. The application of chloral hydrate on the prawn seed under oxygen packing at the selected dose evidently showed a negative effect. A lower dose (300 ppm) than the selected dose, studied separately, also showed similar results under oxygen packing . An increase in packing density decreased the time of initial mortality and percentage survival. At packing densities of 200/1, 400/1, 600/1 and 800/1, the safe durations of transport (duration of 100% survival) were 7.5 h,5 h, 3.5h and 2.5 h respectively at ambient temperature of 29+ 10 C. In P.monodon seed transport the number of seed has been found as more important than their weight in deciding the survival. A high salinity of 25 ppt was found better than a lower salinity for P.monodon seed transport. Lowering of temperature of the packing medium, rather than applying sedatives or lowering of salinity, has been found as a suitable method for increasing the survival during oxygen – packed transport of P.mondon seed. At the lowered temperature of 23+ 20C significantly longer duration of 100% survival (15h at 200/1 and 5 h at 800/1) and better percentage survival at 24 hours (92.5% at 200/1 and 83.13% at 800/1) than at the ambient temperature could be observed.